Restriction map for
pFN21ASDA0526
(Circular) (Six-base) MAPSORT of: pFN21ASDA0526.seq Check: 238 from: 1 to: 6415
pFN21ASDA0526 6415 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2507 3758 1937
Size: 570 1251 4594
Fragments arranged by size:
4594 1251 570
Acc65I G'GTAC_C
Cuts at: 3655 3736 3655
Size: 81 6334
AclI AA'CG_TT
Cuts at: 1563 3149 4542 4915 1563
Size: 1586 1393 373 3063
Fragments arranged by size:
3063 1586 1393 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3027 3503 3653 4478 5862 6130 1632
Size: 1395 476 150 825 1384 268 1917
Fragments arranged by size:
1917 1395 1384 825 476 268 150
AfeI AGC'GCT
Cuts at: 2122 2122
Size: 6415
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 5314 6291 1203
Size: 4111 977 1327
Fragments arranged by size:
4111 1327 977
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 2356 5144 2356
Size: 2788 3627
AleI CACnn'nnGTG
Cuts at: 1166 2246 1166
Size: 1080 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6415
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2979 3011 2979
Size: 32 6383
AlwNI CAG_nnn'CTG
Cuts at: 4158 5730 4158
Size: 1572 4843
ApaI G_GGCC'C
Cuts at: 3083 3083
Size: 6415
ApaLI G'TGCA_C
Cuts at: 4472 5628 4472
Size: 1156 5259
ApoI r'AATT_y
Cuts at: 1493 2746 2949 3724 3950 4102 6211 1493
Size: 1253 203 775 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 775 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 3740 1870
Size: 73 1179 618 4545
Fragments arranged by size:
4545 1179 618 73
BamHI G'GATC_C
Cuts at: 3118 3745 4112 3118
Size: 627 367 5421
Fragments arranged by size:
5421 627 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3071 3314 3461 3655 3736
Size: 325 217 627 1284 243 147 194 81
Cuts at: 3736 5191 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1284 627 325 243 217 194
147 81
BanII G_rGCy'C
Cuts at: 1391 2037 3083 3734 1391
Size: 646 1046 651 4072
Fragments arranged by size:
4072 1046 651 646
BbeI G_GCGC'C
Cuts at: 3075 3465 3075
Size: 390 6025
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2993 3420 3711 928
Size: 971 1094 427 291 3632
Fragments arranged by size:
3632 1094 971 427 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4307 5517 4307
Size: 1210 5205
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6415
BfrBI ATG'CAT
Cuts at: 2813 2813
Size: 6415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3087 5026 136
Size: 107 122 71 631 605 1415 1939 1525
Fragments arranged by size:
1939 1525 1415 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 3083 4476 5632 1790
Size: 1293 1393 1156 2573
Fragments arranged by size:
2573 1393 1293 1156
BmgBI CAC'GTC
Cuts at: 1318 3249 1318
Size: 1931 4484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4146 5104 442
Size: 416 53 83 399 24 2729 958 1753
Fragments arranged by size:
2729 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2496 2496
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 2646 3195 3405 5075 1239
Size: 1230 177 549 210 1670 2579
Fragments arranged by size:
2579 1670 1230 549 210 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2314 2630 3005 4540 5405 5703 2314
Size: 316 375 1535 865 298 3026
Fragments arranged by size:
3026 1535 865 375 316 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5078 882
Size: 617 3579 2219
Fragments arranged by size:
3579 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 2244 3712 4206 493
Size: 711 906 134 1468 494 2702
Fragments arranged by size:
2702 1468 906 711 494 134
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3072 3376 3462 4222
Size: 53 83 186 936 1539 304 86 760
Cuts at: 4222 4604 5239 275
Size: 382 635 1451
Fragments arranged by size:
1539 1451 936 760 635 382 304 186
86 83 53
BsaWI w'CCGG_w
Cuts at: 4848 5246 5520 5667 6078 4848
Size: 398 274 147 411 5185
Fragments arranged by size:
5185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2462 2472 2492 2613
Size: 30 165 30 1039 20 10 20 121
Cuts at: 2613 2643 1178
Size: 30 4950
Fragments arranged by size:
4950 1039 165 121 30 30 30 20
20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2627 2696 1186
Size: 942 499 69 4905
Fragments arranged by size:
4905 942 499 69
BseYI C'CCAG_C
Cuts at: 5618 6114 5618
Size: 496 5919
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2653 3255 1163
Size: 573 917 602 4323
Fragments arranged by size:
4323 917 602 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 3865 4626 5654 1540
Size: 275 186 29 1835 761 1028 2301
Fragments arranged by size:
2301 1835 1028 761 275 186 29
BsiHKAI G_wGCw'C
Cuts at: 2241 4476 4561 5632 2241
Size: 2235 85 1071 3024
Fragments arranged by size:
3024 2235 1071 85
BsmI GAATG_Cn'
Cuts at: 3926 4019 3926
Size: 93 6322
BsmBI CGTCTCn'nnnn_
Cuts at: 3367 3677 3367
Size: 310 6105
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2241 3083 3734 4476 4561 5632
Size: 399 247 204 842 651 742 85 1071
Cuts at: 5632 1391
Size: 2174
Fragments arranged by size:
2174 1071 842 742 651 399 247 204
85
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 2482 4304 6034 2482
Size: 1822 1730 2863
Fragments arranged by size:
2863 1822 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3222 3770 844
Size: 2378 548 3489
Fragments arranged by size:
3489 2378 548
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4910 5084 65
Size: 1183 3662 174 1396
Fragments arranged by size:
3662 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 5059 5246 1700
Size: 110 337 2912 187 2869
Fragments arranged by size:
2912 2869 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 2400 3337 96
Size: 2304 937 3174
Fragments arranged by size:
3174 2304 937
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2781 3242 794
Size: 1987 461 3967
Fragments arranged by size:
3967 1987 461
BstBI TT'CG_AA
Cuts at: 2007 5219 2007
Size: 3212 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 2154 2987 3118 3745 4112 4497 4514 5966
Size: 182 833 131 627 367 385 17 1452
Cuts at: 5966 6052 6064 6410 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 833 627 385 367 346 182
131 86 17 12
BstZ17I GTA'TAC
Cuts at: 2508 2508
Size: 6415
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5863
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5676
Fragments arranged by size:
5676 683 56
BtsI GCAGTG_nn'
Cuts at: 3243 3931 4725 4745 3243
Size: 688 794 20 4913
Fragments arranged by size:
4913 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 3597 3719 3597
Size: 122 6293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2149 2429
Size: 54 1425 50 275 18 197 122 280
Cuts at: 2429 3862 4751 6380 8
Size: 1433 889 1629 43
Fragments arranged by size:
1629 1433 1425 889 280 275 197 122
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 3862 1537
Size: 275 215 1835 4090
Fragments arranged by size:
4090 1835 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 4345 1353
Size: 2992 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5002 5376 5522 387
Size: 1064 138 3413 374 146 1280
Fragments arranged by size:
3413 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2646 3027 3195 3405 3503 3653
Size: 393 837 177 381 168 210 98 150
Cuts at: 3653 4478 5075 5862 6130 1239
Size: 825 597 787 268 1524
Fragments arranged by size:
1524 837 825 787 597 393 381 268
210 177 168 150 98
EcoO109I rG'GnC_Cy
Cuts at: 2937 3080 3619 3672 2937
Size: 143 539 53 5680
Fragments arranged by size:
5680 539 143 53
EcoRI G'AATT_C
Cuts at: 2746 3724 4102 2746
Size: 978 378 5059
Fragments arranged by size:
5059 978 378
EcoRV GAT'ATC
Cuts at: 2279 2279
Size: 6415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3599 3058
Size: 541 5874
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 2124 3075 3465 5562 1678
Size: 446 951 390 2097 2531
Fragments arranged by size:
2531 2097 951 446 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2462 2494 5056
Size: 32 135 32 770 32 88 32 2562
Cuts at: 5056 5088 5130 5162 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2562 770 135 88 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2205 748
Size: 1457 4958
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KasI G'GCGC_C
Cuts at: 3071 3461 3071
Size: 390 6025
KpnI G_GTAC'C
Cuts at: 3659 3740 3659
Size: 81 6334
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3490 5529 5713 1305
Size: 2185 2039 184 2007
Fragments arranged by size:
2185 2039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4593
Fragments arranged by size:
4593 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2960 3287 4373 4732 4891 518
Size: 648 1080 714 327 1086 359 159 2042
Fragments arranged by size:
2042 1086 1080 714 648 359 327 159
MspA1I CmG'CkG
Cuts at: 1606 3631 3831 4508 5656 5901 1606
Size: 2025 200 677 1148 245 2120
Fragments arranged by size:
2120 2025 1148 677 245 200
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 6415
NarI GG'CG_CC
Cuts at: 3072 3462 3072
Size: 390 6025
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5863
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NsiI A_TGCA'T
Cuts at: 2815 2815
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 3448 3773 5318 1132
Size: 97 2219 325 1545 2229
Fragments arranged by size:
2229 2219 1545 325 97
PasI CC'CwG_GG
Cuts at: 1348 3076 1348
Size: 1728 4687
PciI A'CATG_T
Cuts at: 5314 5314
Size: 6415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6415
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3243 4484 4516 5997 6029 3211
Size: 32 1241 32 1481 32 3597
Fragments arranged by size:
3597 1481 1241 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3619 3619
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 2490 2534 3985 2490
Size: 44 1451 4920
Fragments arranged by size:
4920 1451 44
PspOMI G'GGCC_C
Cuts at: 3079 3079
Size: 6415
PstI C_TGCA'G
Cuts at: 830 2128 3767 830
Size: 1298 1639 3478
Fragments arranged by size:
3478 1639 1298
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
PvuII CAG'CTG
Cuts at: 3631 3631
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 2638 3107 4663 6375 2638
Size: 469 1556 1712 2678
Fragments arranged by size:
2678 1712 1556 469
SexAI A'CCwGG_T
Cuts at: 3359 3359
Size: 6415
SfcI C'TryA_G
Cuts at: 826 1045 2124 2437 3264 3763 4898 5579 5770
Size: 219 1079 313 827 499 1135 681 191
Cuts at: 5770 826
Size: 1471
Fragments arranged by size:
1471 1135 1079 827 681 499 313 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3087 3087
Size: 6415
SfoI GGC'GCC
Cuts at: 3073 3463 3073
Size: 390 6025
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2293 2609 3020 4519 5420 5682
Size: 197 926 350 316 411 1499 901 262
Cuts at: 5682 820
Size: 1553
Fragments arranged by size:
1553 1499 926 901 411 350 316 262
197
SnaBI TAC'GTA
Cuts at: 493 3712 4206 493
Size: 3219 494 2702
Fragments arranged by size:
3219 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3773 1132
Size: 2641 3774
SspI AAT'ATT
Cuts at: 5 52 4339 5
Size: 47 4287 2081
Fragments arranged by size:
4287 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 3322 3854 513
Size: 552 2257 532 3074
Fragments arranged by size:
3074 2257 552 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4442 4459 4612 4797 5242 325
Size: 1255 2862 17 153 185 445 1498
Fragments arranged by size:
2862 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2636 3105
Size: 274 80 33 51 1443 423 236 469
Cuts at: 3105 3337 4661 6373 96
Size: 232 1324 1712 138
Fragments arranged by size:
1712 1443 1324 469 423 274 236 232
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 2613 2645 3481 3513 6375 6407 2442
Size: 32 139 32 836 32 2862 32 2450
Fragments arranged by size:
2862 2450 836 139 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 3223 4544 3223
Size: 1321 5094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI
Bsu36I DraIII EcoICRI EcoNI FseI FspAI NruI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SrfI SwaI XcmI