Restriction map for
pFN21ASDA0513
(Circular) (Six-base) MAPSORT of: pFN21ASDA0513.seq Check: 9475 from: 1 to: 5962
pFN21ASDA0513 5962 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2279 3305 1937
Size: 342 1026 4594
Fragments arranged by size:
4594 1026 342
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2735 2798 2927 3194 4025 5409 5677 1632
Size: 1103 63 129 267 831 1384 268 1917
Fragments arranged by size:
1917 1384 1103 831 268 267 129 63
AfeI AGC'GCT
Cuts at: 2453 2453
Size: 5962
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2523 1184
Size: 1339 4623
AlwNI CAG_nnn'CTG
Cuts at: 2611 3705 5277 2611
Size: 1094 1572 3296
Fragments arranged by size:
3296 1572 1094
ApaI G_GGCC'C
Cuts at: 2787 2787
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2625 2861 3287 1870
Size: 73 682 236 426 4545
Fragments arranged by size:
4545 682 426 236 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2428 2461 2428
Size: 33 5929
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2098 3042 3283 4738
Size: 325 217 627 287 24 944 241 1455
Cuts at: 4738 618
Size: 1842
Fragments arranged by size:
1842 1455 944 627 325 287 241 217
24
BanII G_rGCy'C
Cuts at: 1391 2051 2787 3281 1391
Size: 660 736 494 4072
Fragments arranged by size:
4072 736 660 494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2814 928
Size: 971 915 4076
Fragments arranged by size:
4076 971 915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2923 4573 136
Size: 107 122 71 631 605 1251 1650 1525
Fragments arranged by size:
1650 1525 1251 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2337 2886 5957 2337
Size: 549 3071 2342
Fragments arranged by size:
3071 2342 549
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2449 2787 3116 4023 5179 1790
Size: 659 338 329 907 1156 2573
Fragments arranged by size:
2573 1156 907 659 338 329
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2272 2221
Size: 51 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2367 2786 3033 4622 1239
Size: 875 253 419 247 1589 2579
Fragments arranged by size:
2579 1589 875 419 253 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 2153 2288 2762 4625 882
Size: 617 522 132 135 474 1863 2219
Fragments arranged by size:
2219 1863 617 522 474 135 132
BsaAI yAC'GTr
Cuts at: 493 1204 2329 2440 3259 3753 493
Size: 711 1125 111 819 494 2702
Fragments arranged by size:
2702 1125 819 711 494 111
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3078 4395 4793 5067 5214 5625 3078
Size: 1317 398 274 147 411 3415
Fragments arranged by size:
3415 1317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2071 2101 3026 3056 1178
Size: 30 165 30 668 30 925 30 4084
Fragments arranged by size:
4084 925 668 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 2248 2384 3032 3035 3038 3074 1186
Size: 1044 18 136 648 3 3 36 4074
Fragments arranged by size:
4074 1044 648 136 36 18 3 3
BseYI C'CCAG_C
Cuts at: 2448 3109 5165 5661 2448
Size: 661 2056 496 2749
Fragments arranged by size:
2749 2056 661 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 1163
Size: 573 783 4606
Fragments arranged by size:
4606 783 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2273 4023 4108 5179 2273
Size: 1750 85 1071 3056
Fragments arranged by size:
3056 1750 1071 85
BsmI GAATG_Cn'
Cuts at: 2950 3473 3566 2950
Size: 523 93 5346
Fragments arranged by size:
5346 523 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2273 2449 2787 3116 3281 4023
Size: 399 261 222 176 338 329 165 742
Cuts at: 4023 4108 5179 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 329 261 222
176 165 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2565 3851 5581 2565
Size: 1286 1730 2946
Fragments arranged by size:
2946 1730 1286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3317 844
Size: 2473 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2707 3849 1394
Size: 429 884 1142 3507
Fragments arranged by size:
3507 1142 884 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3169 4457 4631 65
Size: 1183 1921 1288 174 1396
Fragments arranged by size:
1921 1396 1288 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 2895 96
Size: 2799 3163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3130 794
Size: 2336 3626
BstBI TT'CG_AA
Cuts at: 2007 4766 2007
Size: 2759 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2337 2886 3292 3659 4044 4061 5513 5599
Size: 365 549 406 367 385 17 1452 86
Cuts at: 5599 5611 5957 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 549 406 385 367 365 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2070 2070
Size: 5962
BtgI C'CryG_G
Cuts at: 513 1065 2022 2043 2637 513
Size: 552 957 21 594 3838
Fragments arranged by size:
3838 957 594 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2139 2683 487
Size: 683 56 913 544 3766
Fragments arranged by size:
3766 913 683 544 56
BtsI GCAGTG_nn'
Cuts at: 2407 2517 3478 4272 4292 2407
Size: 110 961 794 20 4077
Fragments arranged by size:
4077 961 794 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2167 4969 809
Size: 507 851 2802 1802
Fragments arranged by size:
2802 1802 851 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 2727 2790
Size: 54 1425 50 275 18 852 45 63
Cuts at: 2790 3246 3409 4298 5927 8
Size: 456 163 889 1629 43
Fragments arranged by size:
1629 1425 889 852 456 275 163 63
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2089 2286 3023 3201 3892 1353
Size: 736 197 737 178 691 3423
Fragments arranged by size:
3423 737 736 691 197 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2367 2735 2786 2798 2927 3033
Size: 393 482 253 368 51 12 129 106
Cuts at: 3033 3194 4025 4622 5409 5677 1239
Size: 161 831 597 787 268 1524
Fragments arranged by size:
1524 831 787 597 482 393 368 268
253 161 129 106 51 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2728 2728
Size: 5962
EcoO109I rG'GnC_Cy
Cuts at: 2038 2783 2784 2038
Size: 745 1 5216
Fragments arranged by size:
5216 745 1
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2455 5109 1678
Size: 777 2654 2531
Fragments arranged by size:
2654 2531 777
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 4603 4635 4677
Size: 32 135 32 560 32 2439 32 42
Cuts at: 4677 4709 1373
Size: 32 2626
Fragments arranged by size:
2626 2439 560 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 2286 5076 5260 1305
Size: 963 18 2790 184 2007
Fragments arranged by size:
2790 2007 963 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2792 3248 10
Size: 54 1425 343 960 456 2724
Fragments arranged by size:
2724 1425 960 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 3920 4279 4438 518
Size: 648 1404 1350 359 159 2042
Fragments arranged by size:
2042 1404 1350 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2608 2639 2676 3109 3378 4055 5203 5448
Size: 1002 31 37 433 269 677 1148 245
Cuts at: 5448 1606
Size: 2120
Fragments arranged by size:
2120 1148 1002 677 433 269 245 37
31
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4453
Fragments arranged by size:
4453 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 3133 3320 4865 1132
Size: 97 1904 187 1545 2229
Fragments arranged by size:
2229 1904 1545 187 97
PasI CC'CwG_GG
Cuts at: 1348 2134 2838 1348
Size: 786 704 4472
Fragments arranged by size:
4472 786 704
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2038
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2783 2783
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2118 2399 3314 830
Size: 1288 281 915 3478
Fragments arranged by size:
3478 1288 915 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2608 2676 3109 2608
Size: 68 433 5461
Fragments arranged by size:
5461 433 68
SacII CC_GC'GG
Cuts at: 2640 2640
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SapI GCTCTTCn'nnn_
Cuts at: 3201 3201
Size: 5962
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 2393 4210 5922 2393
Size: 1817 1712 2433
Fragments arranged by size:
2433 1817 1712
SfcI C'TryA_G
Cuts at: 826 1045 2114 2395 3310 4445 5126 5317 826
Size: 219 1069 281 915 1135 681 191 1471
Fragments arranged by size:
1471 1135 1069 915 681 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2627 3289 1872
Size: 755 662 4545
Fragments arranged by size:
4545 755 662
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3259 3753 493
Size: 2766 494 2702
Fragments arranged by size:
2766 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2022 3401 513
Size: 552 957 1379 3074
Fragments arranged by size:
3074 1379 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3989 4006 4159 4344 4789 325
Size: 1255 512 1897 17 153 185 445 1498
Fragments arranged by size:
1897 1498 1255 512 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 2895 4208
Size: 274 80 33 51 1443 414 504 1313
Cuts at: 4208 5920 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1313 504 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 3026 3058 5922 5954 2071
Size: 32 923 32 2864 32 2079
Fragments arranged by size:
2864 2079 923 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2963 1316
Size: 1647 4315
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2625 3287 1870
Size: 755 662 4545
Fragments arranged by size:
4545 755 662
XmnI GAAnn'nnTTC
Cuts at: 2339 4091 2339
Size: 1752 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI XcmI