Restriction map for
pFN21ASDA0482
(Circular) (Six-base) MAPSORT of: pFN21ASDA0482.seq Check: 2156 from: 1 to: 8599
pFN21ASDA0482 8599 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2533 2533
Size: 8599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6409 278
Size: 53 83 186 936 4873 2468
Fragments arranged by size:
4873 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 5942 1937
Size: 781 3224 4594
Fragments arranged by size:
4594 3224 781
Acc65I G'GTAC_C
Cuts at: 2774 2958 3930 5920 2774
Size: 184 972 1990 5453
Fragments arranged by size:
5453 1990 972 184
AclI AA'CG_TT
Cuts at: 1563 6726 7099 1563
Size: 5163 373 3063
Fragments arranged by size:
5163 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2648 4177 4593 4872 5510 6662 8046 8314
Size: 1016 1529 416 279 638 1152 1384 268
Cuts at: 8314 1632
Size: 1917
Fragments arranged by size:
1917 1529 1384 1152 1016 638 416 279
268
AfeI AGC'GCT
Cuts at: 4077 4544 4077
Size: 467 8132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8402
AflIII A'CryG_T
Cuts at: 1203 2610 2763 3429 7498 8475 1203
Size: 1407 153 666 4069 977 1327
Fragments arranged by size:
4069 1407 1327 977 666 153
AgeI A'CCGG_T
Cuts at: 7430 7430
Size: 8599
AhdI GACnn_n'nnGTC
Cuts at: 2377 7328 2377
Size: 4951 3648
AleI CACnn'nnGTG
Cuts at: 1166 3025 4733 1166
Size: 1859 1708 5032
Fragments arranged by size:
5032 1859 1708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3043 5899 1184
Size: 1859 2856 3884
Fragments arranged by size:
3884 2856 1859
AlwNI CAG_nnn'CTG
Cuts at: 2391 2690 3487 4010 4043 6342 7914 2391
Size: 299 797 523 33 2299 1572 3076
Fragments arranged by size:
3076 2299 1572 797 523 299 33
ApaI G_GGCC'C
Cuts at: 2112 3016 3196 3730 5052 2112
Size: 904 180 534 1322 5659
Fragments arranged by size:
5659 1322 904 534 180
ApaLI G'TGCA_C
Cuts at: 3356 5573 6656 7812 3356
Size: 2217 1083 1156 4143
Fragments arranged by size:
4143 2217 1156 1083
ApoI r'AATT_y
Cuts at: 1493 5908 6134 6286 8395 1493
Size: 4415 226 152 2109 1697
Fragments arranged by size:
4415 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7153 160
Size: 872 6121 1606
Fragments arranged by size:
6121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8599
AvaI C'yCGr_G
Cuts at: 1870 1943 2412 3723 4566 4695 4909 4948 5200
Size: 73 469 1311 843 129 214 39 252
Cuts at: 5200 5355 5604 5924 1870
Size: 155 249 320 4545
Fragments arranged by size:
4545 1311 843 469 320 252 249 214
155 129 73 39
BamHI G'GATC_C
Cuts at: 2944 5404 5929 6296 2944
Size: 2460 525 367 5247
Fragments arranged by size:
5247 2460 525 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2774 2958 3560 3930 4124
Size: 325 217 627 987 184 602 370 194
Cuts at: 4124 5461 5920 7375 618
Size: 1337 459 1455 1842
Fragments arranged by size:
1842 1455 1337 987 627 602 459 370
325 217 194 184
BanII G_rGCy'C
Cuts at: 1391 2112 2231 2315 2457 2793 3016 3196 3730
Size: 721 119 84 142 336 223 180 534
Cuts at: 3730 4136 4998 5052 5207 5216 5228 5611 5712
Size: 406 862 54 155 9 12 383 101
Cuts at: 5712 5852 5918 1391
Size: 140 66 4072
Fragments arranged by size:
4072 862 721 534 406 383 336 223
180 155 142 140 119 101 84 66
54 12 9
BbeI G_GCGC'C
Cuts at: 3564 3564
Size: 8599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2809 2905 928
Size: 971 910 96 6622
Fragments arranged by size:
6622 971 910 96
BbvCI CC'TCA_GC
Cuts at: 2869 4003 4406 4465 5007 5266 5340 2869
Size: 1134 403 59 542 259 74 6128
Fragments arranged by size:
6128 1134 542 403 259 74 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4506 4540 5516 5550 6790 6824 1903
Size: 34 2569 34 976 34 1240 34 3678
Fragments arranged by size:
3678 2569 1240 976 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2668 3072 6491 7701 2668
Size: 404 3419 1210 3566
Fragments arranged by size:
3566 3419 1210 404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4154 4494 5093
Size: 107 122 71 631 605 2482 340 599
Cuts at: 5093 7210 136
Size: 2117 1525
Fragments arranged by size:
2482 2117 1525 631 605 599 340 122
107 71
BglII A'GATC_T
Cuts at: 3666 8594 3666
Size: 4928 3671
BlpI GC'TnA_GC
Cuts at: 5076 5367 6016 5076
Size: 291 649 7659
Fragments arranged by size:
7659 649 291
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2861 3016 3196 3310 3360 3730 4740
Size: 322 749 155 180 114 50 370 1010
Cuts at: 4740 4837 5052 5466 5577 6660 7816 1790
Size: 97 215 414 111 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 1010 749 414 370 322
215 180 155 114 111 97 50
BmgBI CAC'GTC
Cuts at: 1318 2649 2764 3430 1318
Size: 1331 115 666 6487
Fragments arranged by size:
6487 1331 666 115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3191 4676 5055
Size: 416 53 83 399 24 1774 1485 379
Cuts at: 5055 5573 5708 6330 7288 442
Size: 518 135 622 958 1753
Fragments arranged by size:
1774 1753 1485 958 622 518 416 399
379 135 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 4935 2617
Size: 2318 6281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2603 2648 2657 3385 3586 3833 3868
Size: 1055 309 45 9 728 201 247 35
Cuts at: 3868 3956 4823 4952 5009 5122 5384 5452 5726
Size: 88 867 129 57 113 262 68 274
Cuts at: 5726 7259 1239
Size: 1533 2579
Fragments arranged by size:
2579 1533 1055 867 728 309 274 262
247 201 129 113 88 68 57 45
35 9
Bpu10I CC'TnA_GC
Cuts at: 2869 4003 4130 4406 4465 5007 5266 5340 2869
Size: 1134 127 276 59 542 259 74 6128
Fragments arranged by size:
6128 1134 542 276 259 127 74 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2441 6724 7589 7887 2441
Size: 4283 865 298 3153
Fragments arranged by size:
4283 3153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 3007 4413 7262 882
Size: 617 771 737 1406 2849 2219
Fragments arranged by size:
2849 2219 1406 771 737 617
BsaAI yAC'GTr
Cuts at: 493 1204 5395 5896 6390 493
Size: 711 4191 501 494 2702
Fragments arranged by size:
4191 2702 711 501 494
BsaBI GATnn'nnATC
Cuts at: 3681 8593 3681
Size: 4912 3687
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 3561 4335 6406
Size: 53 83 186 936 561 1467 774 2071
Cuts at: 6406 6788 7423 275
Size: 382 635 1451
Fragments arranged by size:
2071 1467 1451 936 774 635 561 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5586 7032 7430 7704 7851 8262 5586
Size: 1446 398 274 147 411 5923
Fragments arranged by size:
5923 1446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 5162 5192 1178
Size: 30 165 30 822 30 2907 30 4585
Fragments arranged by size:
4585 2907 822 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 2233 2600 2645 2943 3875 3970 3973
Size: 1044 3 367 45 298 932 95 3
Cuts at: 3973 3976 3991 4683 4713 4908 5006 5792 5843
Size: 3 15 692 30 195 98 786 51
Cuts at: 5843 5846 1186
Size: 3 3939
Fragments arranged by size:
3939 1044 932 786 692 367 298 195
98 95 51 45 30 15 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2138 2324 3119 3163 3342 3449 3454 3533 3953
Size: 186 795 44 179 107 5 79 420
Cuts at: 3953 4282 4289 4294 4301 4324 4654 4707 4741
Size: 329 7 5 7 23 330 53 34
Cuts at: 4741 5345 7802 8298 2138
Size: 604 2457 496 2439
Fragments arranged by size:
2457 2439 795 604 496 420 330 329
186 179 107 79 53 44 34 23
7 7 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 3003 4361 5869 1163
Size: 573 831 436 1358 1508 3893
Fragments arranged by size:
3893 1508 1358 831 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6049 6810 7838 1540
Size: 275 186 4048 761 1028 2301
Fragments arranged by size:
4048 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2231 2300 2457 2643 2793 3204 3360 4791 4998
Size: 69 157 186 150 411 156 1431 207
Cuts at: 4998 5400 5577 5852 6660 6745 7816 2231
Size: 402 177 275 808 85 1071 3014
Fragments arranged by size:
3014 1431 1071 808 411 402 275 207
186 177 157 156 150 85 69
BsmI GAATG_Cn'
Cuts at: 6110 6203 6110
Size: 93 8506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2231 2300 2315 2457 2643 2793
Size: 399 322 119 69 15 142 186 150
Cuts at: 2793 2861 3016 3196 3204 3310 3360 3730 4136
Size: 68 155 180 8 106 50 370 406
Cuts at: 4136 4740 4791 4837 4998 5052 5207 5216 5228
Size: 604 51 46 161 54 155 9 12
Cuts at: 5228 5400 5466 5577 5611 5712 5852 5918 6660
Size: 172 66 111 34 101 140 66 742
Cuts at: 6660 6745 7816 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 406 399 370 322
186 180 172 161 155 155 150 142
140 119 111 106 101 85 69 68
66 66 54 51 50 46 34 15
12 9 8
BspEI T'CCGG_A
Cuts at: 5586 8262 5586
Size: 2676 5923
BspHI T'CATG_A
Cuts at: 2436 4248 5445 6488 8218 2436
Size: 1812 1197 1043 1730 2817
Fragments arranged by size:
2817 1812 1730 1197 1043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2533 2864 3180 4269 5154 5954 844
Size: 1689 331 316 1089 885 800 3489
Fragments arranged by size:
3489 1689 1089 885 800 331 316
BsrBI CCG'CTC
Cuts at: 1394 1823 2496 4138 5506 6486 1394
Size: 429 673 1642 1368 980 3507
Fragments arranged by size:
3507 1642 1368 980 673 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2176 2191 2334 7094 7268 65
Size: 1183 928 15 143 4760 174 1396
Fragments arranged by size:
4760 1396 1183 928 174 143 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 3504 7243 7430 1700
Size: 110 311 1383 3739 187 2869
Fragments arranged by size:
3739 2869 1383 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8599
BssSI C'ACGA_G
Cuts at: 6659 7671 6659
Size: 1012 7587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8599
BstBI TT'CG_AA
Cuts at: 2007 7403 2007
Size: 5396 3203
BstEII G'GTnAC_C
Cuts at: 1184 4765 5660 1184
Size: 3581 895 4123
Fragments arranged by size:
4123 3581 895
BstXI CCAn_nnnn'nTGG
Cuts at: 3593 5657 3593
Size: 2064 6535
BstYI r'GATC_y
Cuts at: 1972 2944 2994 3498 3666 4060 5404 5653 5929
Size: 972 50 504 168 394 1344 249 276
Cuts at: 5929 6296 6681 6698 8150 8236 8248 8594 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1344 972 504 394 385 367
346 276 249 168 86 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2245 2885 3219 3858 3926 2245
Size: 640 334 639 68 6918
Fragments arranged by size:
6918 640 639 334 68
BtgI C'CryG_G
Cuts at: 513 1065 2604 3330 3540 4100 4492 5712 513
Size: 552 1539 726 210 560 392 1220 3400
Fragments arranged by size:
3400 1539 1220 726 560 552 392 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7860
Fragments arranged by size:
7860 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 2579 2829 4522 5047 5761 6115 6909 6929
Size: 175 250 1693 525 714 354 794 20
Cuts at: 6929 2404
Size: 4074
Fragments arranged by size:
4074 1693 794 714 525 354 250 175
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3831 3866 529
Size: 35 3267 35 5262
Fragments arranged by size:
5262 3267 35 35
DraI TTT'AAA
Cuts at: 5903 5903
Size: 8599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5441 7606 809
Size: 507 4125 2165 1802
Fragments arranged by size:
4125 2165 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2508 4146
Size: 54 1425 50 275 18 315 363 1638
Cuts at: 4146 5424 6046 6935 8564 8
Size: 1278 622 889 1629 43
Fragments arranged by size:
1638 1629 1425 1278 889 622 363 315
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6046 1537
Size: 275 4234 4090
Fragments arranged by size:
4234 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3134 4386 4456 4719 5335 5684 5712 6529
Size: 1781 1252 70 263 616 349 28 817
Cuts at: 6529 1353
Size: 3423
Fragments arranged by size:
3423 1781 1252 817 616 349 263 70
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2960 4053 4172 5111 7186 7560
Size: 1064 138 1371 1093 119 939 2075 374
Cuts at: 7560 7706 387
Size: 146 1280
Fragments arranged by size:
2075 1371 1280 1093 1064 939 374 146
138 119
EcoICRI GAG'CTC
Cuts at: 2229 2455 2791 4996 5850 2229
Size: 226 336 2205 854 4978
Fragments arranged by size:
4978 2205 854 336 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2603 2648 2657 3385 3586 3833
Size: 393 662 309 45 9 728 201 247
Cuts at: 3833 3868 3956 4177 4593 4823 4872 4952 5009
Size: 35 88 221 416 230 49 80 57
Cuts at: 5009 5122 5384 5452 5510 5726 6662 7259 8046
Size: 113 262 68 58 216 936 597 787
Cuts at: 8046 8314 1239
Size: 268 1524
Fragments arranged by size:
1524 936 787 728 662 597 416 393
309 268 262 247 230 221 216 201
113 88 80 68 58 57 49 45
35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2130 2585 3131 3176 3422 3759 4698 4851 5874
Size: 455 546 45 246 337 939 153 1023
Cuts at: 5874 2130
Size: 4855
Fragments arranged by size:
4855 1023 939 546 455 337 246 153
45
EcoO109I rG'GnC_Cy
Cuts at: 2059 2109 2135 2502 2865 2933 2951 3012 3013
Size: 50 26 367 363 68 18 61 1
Cuts at: 3013 3181 3192 3193 3578 3597 3632 3781 3838
Size: 168 11 1 385 19 35 149 57
Cuts at: 3838 4258 4703 4856 5030 5049 2059
Size: 420 445 153 174 19 5609
Fragments arranged by size:
5609 445 420 385 367 363 174 168
153 149 68 61 57 50 35 26
19 19 18 11 1 1
EcoRI G'AATT_C
Cuts at: 5908 6286 5908
Size: 378 8221
EcoRV GAT'ATC
Cuts at: 3478 3478
Size: 8599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 3397 2929
Size: 468 8131
FspI TGC'GCA
Cuts at: 7105 7105
Size: 8599
HaeII r_GCGC'y
Cuts at: 1678 2482 3564 4079 4546 4580 7746 1678
Size: 804 1082 515 467 34 3166 2531
Fragments arranged by size:
3166 2531 1082 804 515 467 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2666 2698 4018
Size: 32 135 32 791 32 271 32 1320
Cuts at: 4018 4050 4052 4084 5162 5194 5465 5497 7240
Size: 32 2 32 1078 32 271 32 1743
Cuts at: 7240 7272 7314 7346 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1743 1320 1078 791 271 271 135
42 32 32 32 32 32 32 32
32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5943 6189 669
Size: 988 281 4005 246 3079
Fragments arranged by size:
4005 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2459 748
Size: 1711 6888
HpaI GTT'AAC
Cuts at: 6189 6189
Size: 8599
KasI G'GCGC_C
Cuts at: 3560 3560
Size: 8599
KpnI G_GTAC'C
Cuts at: 2778 2962 3934 5924 2778
Size: 184 972 1990 5453
Fragments arranged by size:
5453 1990 972 184
MfeI C'AATT_G
Cuts at: 6198 6198
Size: 8599
MluI A'CGCG_T
Cuts at: 8475 8475
Size: 8599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2217 4218 7713 7897 1305
Size: 912 2001 3495 184 2007
Fragments arranged by size:
3495 2007 2001 912 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 4148 5426 10
Size: 54 1425 343 678 1638 1278 3183
Fragments arranged by size:
3183 1638 1425 1278 678 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 3025 4247 4250 4733 5667 6557
Size: 648 1443 416 1222 3 483 934 890
Cuts at: 6557 6916 7075 518
Size: 359 159 2042
Fragments arranged by size:
2042 1443 1222 934 890 648 483 416
359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2224 3123 3342 3888 4658 4745 4987 5554
Size: 618 899 219 546 770 87 242 567
Cuts at: 5554 5745 6015 6692 7840 8085 1606
Size: 191 270 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 770 677 618 567 546
270 245 242 219 191 87
NarI GG'CG_CC
Cuts at: 3561 3561
Size: 8599
NcoI C'CATG_G
Cuts at: 513 1065 2604 3330 5712 513
Size: 552 1539 726 2382 3400
Fragments arranged by size:
3400 2382 1539 726 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8599
NotI GC'GGCC_GC
Cuts at: 6046 6046
Size: 8599
NspI r_CATG'y
Cuts at: 1132 1229 2614 5957 7502 1132
Size: 97 1385 3343 1545 2229
Fragments arranged by size:
3343 2229 1545 1385 97
PasI CC'CwG_GG
Cuts at: 1348 2105 3593 5033 1348
Size: 757 1488 1440 4914
Fragments arranged by size:
4914 1488 1440 757
PciI A'CATG_T
Cuts at: 2610 7498 2610
Size: 4888 3711
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2105 2809 3139 4623 1108
Size: 997 704 330 1484 5084
Fragments arranged by size:
5084 1484 997 704 330
PfoI T'CCnGG_A
Cuts at: 3132 3480 5400 3132
Size: 348 1920 6331
Fragments arranged by size:
6331 1920 348
PmeI GTTT'AAAC
Cuts at: 5903 5903
Size: 8599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 6668 6700 8181 8213 3416
Size: 32 3220 32 1481 32 3802
Fragments arranged by size:
3802 3220 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2502 2933 3181 3597 4258 2059
Size: 443 431 248 416 661 6400
Fragments arranged by size:
6400 661 443 431 416 248
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8599
PsiI TTA'TAA
Cuts at: 6169 6169
Size: 8599
PspOMI G'GGCC_C
Cuts at: 2108 3012 3192 3726 5048 2108
Size: 904 180 534 1322 5659
Fragments arranged by size:
5659 1322 904 534 180
PspXI vC'TCGA_Gb
Cuts at: 4695 4695
Size: 8599
PstI C_TGCA'G
Cuts at: 830 2396 2520 3264 3519 4986 5951 830
Size: 1566 124 744 255 1467 965 3478
Fragments arranged by size:
3478 1566 1467 965 744 255 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8599
PvuII CAG'CTG
Cuts at: 3123 3342 3888 4745 4987 3123
Size: 219 546 857 242 6735
Fragments arranged by size:
6735 857 546 242 219
SacI G_AGCT'C
Cuts at: 2231 2457 2793 4998 5852 2231
Size: 226 336 2205 854 4978
Fragments arranged by size:
4978 2205 854 336 226
SalI G'TCGA_C
Cuts at: 1936 5941 1936
Size: 4005 4594
SanDI GG'GwC_CC
Cuts at: 2059 2502 2059
Size: 443 8156
SapI GCTCTTCn'nnn_
Cuts at: 4386 4456 4386
Size: 70 8529
SbfI CC_TGCA'GG
Cuts at: 5951 5951
Size: 8599
ScaI AGT'ACT
Cuts at: 6847 8559 6847
Size: 1712 6887
SexAI A'CCwGG_T
Cuts at: 5379 5457 5379
Size: 78 8521
SfcI C'TryA_G
Cuts at: 826 1045 2341 2392 2516 3260 3515 3759 4982
Size: 219 1296 51 124 744 255 244 1223
Cuts at: 4982 5871 5947 7082 7763 7954 826
Size: 889 76 1135 681 191 1471
Fragments arranged by size:
1471 1296 1223 1135 889 744 681 255
244 219 191 124 76 51
SfoI GGC'GCC
Cuts at: 3562 3562
Size: 8599
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8599
SmaI CCC'GGG
Cuts at: 1872 2414 5926 1872
Size: 542 3512 4545
Fragments arranged by size:
4545 3512 542
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2456 4695 4948 6703 7604 7866
Size: 197 926 513 2239 253 1755 901 262
Cuts at: 7866 820
Size: 1553
Fragments arranged by size:
2239 1755 1553 926 901 513 262 253
197
SnaBI TAC'GTA
Cuts at: 493 5896 6390 493
Size: 5403 494 2702
Fragments arranged by size:
5403 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8599
SphI G_CATG'C
Cuts at: 1132 5957 1132
Size: 4825 3774
SrfI GCCC'GGGC
Cuts at: 2414 2414
Size: 8599
SspI AAT'ATT
Cuts at: 5 52 6523 5
Size: 47 6471 2081
Fragments arranged by size:
6471 2081 47
StuI AGG'CCT
Cuts at: 1517 2068 2883 1517
Size: 551 815 7233
Fragments arranged by size:
7233 815 551
StyI C'CwwG_G
Cuts at: 513 1065 2604 3330 3777 3849 4359 4471 4782
Size: 552 1539 726 447 72 510 112 311
Cuts at: 4782 5208 5712 5785 5796 6038 513
Size: 426 504 73 11 242 3074
Fragments arranged by size:
3074 1539 726 552 510 504 447 426
311 242 112 73 72 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 3133 3240 5555 5663 6626 6643
Size: 1255 758 795 107 2315 108 963 17
Cuts at: 6643 6796 6981 7426 325
Size: 153 185 445 1498
Fragments arranged by size:
2315 1498 1255 963 795 758 445 185
153 108 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 6845 8557
Size: 274 80 33 51 1443 678 4190 1712
Cuts at: 8557 96
Size: 138
Fragments arranged by size:
4190 1712 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 4514 4546 5638 5670 8559 8591 2225
Size: 32 2257 32 1092 32 2889 32 2233
Fragments arranged by size:
2889 2257 2233 1092 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8599
XbaI T'CTAG_A
Cuts at: 2207 5935 2207
Size: 3728 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 4210 2623
Size: 1587 7012
XhoI C'TCGA_G
Cuts at: 1943 4695 4948 1943
Size: 2752 253 5594
Fragments arranged by size:
5594 2752 253
XmaI C'CCGG_G
Cuts at: 1870 2412 5924 1870
Size: 542 3512 4545
Fragments arranged by size:
4545 3512 542
XmnI GAAnn'nnTTC
Cuts at: 6728 6728
Size: 8599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6407 276
Size: 53 83 186 936 4873 2468
Fragments arranged by size:
4873 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PsrI RsrII SacII SfiI SwaI