Restriction map for
pFN21ASDA0463
(Circular) (Six-base) MAPSORT of: pFN21ASDA0463.seq Check: 209 from: 1 to: 10456
pFN21ASDA0463 10456 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3871 4136 4548 4741 5363 3871
Size: 265 412 193 622 8964
Fragments arranged by size:
8964 622 412 265 193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3240 6917 8266 278
Size: 53 83 186 936 1704 3677 1349 2468
Fragments arranged by size:
3677 2468 1704 1349 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 3608 5028 5139 6947 7799 1937
Size: 889 782 1420 111 1808 852 4594
Fragments arranged by size:
4594 1808 1420 889 852 782 111
Acc65I G'GTAC_C
Cuts at: 7777 7777
Size: 10456
AclI AA'CG_TT
Cuts at: 1563 8583 8956 1563
Size: 7020 373 3063
Fragments arranged by size:
7020 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 5579 6143 6925 8519 9903 10171 1632
Size: 1107 2840 564 782 1594 1384 268 1917
Fragments arranged by size:
2840 1917 1594 1384 1107 782 564 268
AfeI AGC'GCT
Cuts at: 3545 7189 3545
Size: 3644 6812
AflII C'TTAA_G
Cuts at: 820 1017 4232 820
Size: 197 3215 7044
Fragments arranged by size:
7044 3215 197
AflIII A'CryG_T
Cuts at: 1203 2946 3672 7212 9355 10332 1203
Size: 1743 726 3540 2143 977 1327
Fragments arranged by size:
3540 2143 1743 1327 977 726
AgeI A'CCGG_T
Cuts at: 9287 9287
Size: 10456
AhdI GACnn_n'nnGTC
Cuts at: 2341 2689 9185 2341
Size: 348 6496 3612
Fragments arranged by size:
6496 3612 348
AleI CACnn'nnGTG
Cuts at: 1166 2627 4919 4979 7265 1166
Size: 1461 2292 60 2286 4357
Fragments arranged by size:
4357 2292 2286 1461 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2399 2501 1184
Size: 1215 102 9139
Fragments arranged by size:
9139 1215 102
AlwNI CAG_nnn'CTG
Cuts at: 3304 3782 4456 4599 6362 7702 8199 9771 3304
Size: 478 674 143 1763 1340 497 1572 3989
Fragments arranged by size:
3989 1763 1572 1340 674 497 478 143
ApaI G_GGCC'C
Cuts at: 2098 3921 6485 6982 7669 2098
Size: 1823 2564 497 687 4885
Fragments arranged by size:
4885 2564 1823 687 497
ApaLI G'TGCA_C
Cuts at: 3244 4561 7175 8513 9669 3244
Size: 1317 2614 1338 1156 4031
Fragments arranged by size:
4031 2614 1338 1317 1156
ApoI r'AATT_y
Cuts at: 1493 5297 7765 7991 8143 10252 1493
Size: 3804 2468 226 152 2109 1697
Fragments arranged by size:
3804 2468 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7728 9010 160
Size: 872 6696 1282 1606
Fragments arranged by size:
6696 1606 1282 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10456
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 2679 2846 2970 4961 5190 5859
Size: 73 148 588 167 124 1991 229 669
Cuts at: 5859 6046 6750 6975 7781 1870
Size: 187 704 225 806 4545
Fragments arranged by size:
4545 1991 806 704 669 588 229 225
187 167 148 124 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6004 6037 6004
Size: 33 10423
BamHI G'GATC_C
Cuts at: 5286 7786 8153 5286
Size: 2500 367 7589
Fragments arranged by size:
7589 2500 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 3174 3253 4180 4762
Size: 325 217 627 777 610 79 927 582
Cuts at: 4762 5362 6732 7124 7777 9232 618
Size: 600 1370 392 653 1455 1842
Fragments arranged by size:
1842 1455 1370 927 777 653 627 610
600 582 392 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2098 3216 3635 3921 5149 5955 6485 6982
Size: 707 1118 419 286 1228 806 530 497
Cuts at: 6982 7659 7669 7775 1391
Size: 677 10 106 4072
Fragments arranged by size:
4072 1228 1118 806 707 677 530 497
419 286 106 10
BbeI G_GCGC'C
Cuts at: 3178 3257 6736 3178
Size: 79 3479 6898
Fragments arranged by size:
6898 3479 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 9485
BbvCI CC'TCA_GC
Cuts at: 2695 3977 2695
Size: 1282 9174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3107 3141 8647 8681 1903
Size: 34 1170 34 5506 34 3678
Fragments arranged by size:
5506 3678 1170 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8348 9558 8348
Size: 1210 9246
BclI T'GATC_A
Cuts at: 1548 3538 6987 1548
Size: 1990 3449 5017
Fragments arranged by size:
5017 3449 1990
BfrBI ATG'CAT
Cuts at: 6823 6823
Size: 10456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4272 6252 9067
Size: 107 122 71 631 605 2600 1980 2815
Cuts at: 9067 136
Size: 1525
Fragments arranged by size:
2815 2600 1980 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4194 7092 7623 10451 4194
Size: 2898 531 2828 4199
Fragments arranged by size:
4199 2898 2828 531
BlpI GC'TnA_GC
Cuts at: 3800 4800 5510 5949 7873 3800
Size: 1000 710 439 1924 6383
Fragments arranged by size:
6383 1924 1000 710 439
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2567 3248 3664 3921 4042 4185 4565
Size: 308 469 681 416 257 121 143 380
Cuts at: 4565 4767 6485 6690 6982 7127 7179 7615 7669
Size: 202 1718 205 292 145 52 436 54
Cuts at: 7669 8517 9673 1790
Size: 848 1156 2573
Fragments arranged by size:
2573 1718 1156 848 681 469 436 416
380 308 292 257 205 202 145 143
121 54 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6608 7327 8187
Size: 416 53 83 399 24 5191 719 860
Cuts at: 8187 9145 442
Size: 958 1753
Fragments arranged by size:
5191 1753 958 860 719 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10456
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3502 3837 7645 3502
Size: 335 3808 6313
Fragments arranged by size:
6313 3808 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2901 3230 3303 3986 4324 5541 5610
Size: 881 781 329 73 683 338 1217 69
Cuts at: 5610 5930 5939 6101 6203 6338 9116 1239
Size: 320 9 162 102 135 2778 2579
Fragments arranged by size:
2778 2579 1217 881 781 683 338 329
320 162 135 102 73 69 9
Bpu10I CC'TnA_GC
Cuts at: 2695 3629 3977 5995 6521 2695
Size: 934 348 2018 526 6630
Fragments arranged by size:
6630 2018 934 526 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3479 4496 5864 6266 8581 9446 9744 3479
Size: 1017 1368 402 2315 865 298 4191
Fragments arranged by size:
4191 2315 1368 1017 865 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2676 2779 2843 2877 3490 5621 5648
Size: 617 1177 103 64 34 613 2131 27
Cuts at: 5648 7151 9119 882
Size: 1503 1968 2219
Fragments arranged by size:
2219 2131 1968 1503 1177 617 613 103
64 34 27
BsaAI yAC'GTr
Cuts at: 493 1204 2947 5713 6109 7753 8247 493
Size: 711 1743 2766 396 1644 494 2702
Fragments arranged by size:
2766 2702 1743 1644 711 494 396
BsaBI GATnn'nnATC
Cuts at: 10450 10450
Size: 10456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3175 3237 3254 6733
Size: 53 83 186 936 1642 62 17 3479
Cuts at: 6733 6914 7371 8263 8645 9280 275
Size: 181 457 892 382 635 1451
Fragments arranged by size:
3479 1642 1451 936 892 635 457 382
186 181 83 62 53 17
BsaWI w'CCGG_w
Cuts at: 4521 6348 8889 9287 9561 9708 10119 4521
Size: 1827 2541 398 274 147 411 4858
Fragments arranged by size:
4858 2541 1827 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3662 3692 7433 7463 1178
Size: 30 165 30 2259 30 3741 30 4171
Fragments arranged by size:
4171 3741 2259 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2725 4205 5801 5804 6865 6925 7667
Size: 850 689 1480 1596 3 1061 60 742
Cuts at: 7667 1186
Size: 3975
Fragments arranged by size:
3975 1596 1480 1061 850 742 689 60
3
BseYI C'CCAG_C
Cuts at: 2158 3226 3764 4173 4251 4485 4757 5009 5658
Size: 1068 538 409 78 234 272 252 649
Cuts at: 5658 6135 6696 7432 7481 7494 7531 9659 10155
Size: 477 561 736 49 13 37 2128 496
Cuts at: 10155 2158
Size: 2459
Fragments arranged by size:
2459 2128 1068 736 649 561 538 496
477 409 272 252 234 78 49 37
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 2950 3650 4093 4500 4564 4753
Size: 573 651 563 700 443 407 64 189
Cuts at: 4753 4769 6389 7568 1163
Size: 16 1620 1179 4051
Fragments arranged by size:
4051 1620 1179 700 651 573 563 443
407 189 64 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4522 5143 5799 6214 6704 7906
Size: 275 186 2521 621 656 415 490 1202
Cuts at: 7906 8667 9695 1540
Size: 761 1028 2301
Fragments arranged by size:
2521 2301 1202 1028 761 656 621 490
415 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2388 2538 3216 3248 3495 3635 3680 3789
Size: 358 150 678 32 247 140 45 109
Cuts at: 3789 4565 4634 5619 5955 7179 7428 7659 8517
Size: 776 69 985 336 1224 249 231 858
Cuts at: 8517 8602 9673 2030
Size: 85 1071 2813
Fragments arranged by size:
2813 1224 1071 985 858 776 678 358
336 249 247 231 150 140 109 85
69 45 32
BsmI GAATG_Cn'
Cuts at: 7561 7967 8060 7561
Size: 406 93 9957
Fragments arranged by size:
9957 406 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2048 2048
Size: 10456
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2098 2388 2538 2567 3216 3248
Size: 399 240 68 290 150 29 649 32
Cuts at: 3248 3495 3635 3664 3680 3789 3921 4042 4185
Size: 247 140 29 16 109 132 121 143
Cuts at: 4185 4565 4634 4767 5149 5619 5955 6485 6690
Size: 380 69 133 382 470 336 530 205
Cuts at: 6690 6982 7127 7179 7428 7615 7659 7669 7775
Size: 292 145 52 249 187 44 10 106
Cuts at: 7775 8517 8602 9673 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 530 470 399 382
380 336 292 290 249 247 240 205
187 150 145 143 140 133 132 121
109 106 85 69 68 52 44 32
29 29 16 10
BspEI T'CCGG_A
Cuts at: 6348 10119 6348
Size: 3771 6685
BspHI T'CATG_A
Cuts at: 4114 4890 6237 8345 10075 4114
Size: 776 1347 2108 1730 4495
Fragments arranged by size:
4495 2108 1730 1347 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3871 4136 4548 4741 5054 5172 5363 7811
Size: 3027 265 412 193 313 118 191 2448
Cuts at: 7811 844
Size: 3489
Fragments arranged by size:
3489 3027 2448 412 313 265 193 191
118
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 4322 5994 8343 1394
Size: 429 292 2207 1672 2349 3507
Fragments arranged by size:
3507 2349 2207 1672 429 292
BsrDI GCAATG_nn'
Cuts at: 65 1248 5220 5838 8951 9125 65
Size: 1183 3972 618 3113 174 1396
Fragments arranged by size:
3972 3113 1396 1183 618 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 3792 4991 5798 6242 7428 9100
Size: 110 355 1627 1199 807 444 1186 1672
Cuts at: 9100 9287 1700
Size: 187 2869
Fragments arranged by size:
2869 1672 1627 1199 1186 807 444 355
187 110
BsrGI T'GTAC_A
Cuts at: 96 3339 96
Size: 3243 7213
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10456
BssSI C'ACGA_G
Cuts at: 4832 8516 9528 4832
Size: 3684 1012 5760
Fragments arranged by size:
5760 3684 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4107 794
Size: 3313 7143
BstBI TT'CG_AA
Cuts at: 2007 9260 2007
Size: 7253 3203
BstEII G'GTnAC_C
Cuts at: 1184 3574 4981 7049 7064 1184
Size: 2390 1407 2068 15 4576
Fragments arranged by size:
4576 2390 2068 1407 15
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 4376 5003 5986 2308
Size: 2068 627 983 6778
Fragments arranged by size:
6778 2068 983 627
BstYI r'GATC_y
Cuts at: 1972 2748 4194 5286 6609 7092 7623 7786 8153
Size: 776 1446 1092 1323 483 531 163 367
Cuts at: 8153 8538 8555 10007 10093 10105 10451 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1446 1323 1092 776 531 483
385 367 346 163 86 17 12
BstZ17I GTA'TAC
Cuts at: 3609 6948 3609
Size: 3339 7117
Bsu36I CC'TnA_GG
Cuts at: 2856 4449 2856
Size: 1593 8863
BtgI C'CryG_G
Cuts at: 513 1065 3460 3588 4401 4702 5376 7045 7590
Size: 552 2395 128 813 301 674 1669 545
Cuts at: 7590 513
Size: 3379
Fragments arranged by size:
3379 2395 1669 813 674 552 545 301
128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4780 5776 7504 487
Size: 683 56 3554 996 1728 3439
Fragments arranged by size:
3554 3439 1728 996 683 56
BtsI GCAGTG_nn'
Cuts at: 3762 4042 6717 7972 8766 8786 3762
Size: 280 2675 1255 794 20 5432
Fragments arranged by size:
5432 2675 1255 794 280 20
ClaI AT'CG_AT
Cuts at: 1554 3267 3718 6306 1554
Size: 1713 451 2588 5704
Fragments arranged by size:
5704 2588 1713 451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3303 3338 6769 6804 529
Size: 35 2739 35 3431 35 4181
Fragments arranged by size:
4181 3431 2739 35 35 35
DraI TTT'AAA
Cuts at: 7760 7760
Size: 10456
DraIII CAC_nnn'GTG
Cuts at: 4747 7591 4747
Size: 2844 7612
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4460 5192 6065 9463 809
Size: 507 3144 732 873 3398 1802
Fragments arranged by size:
3398 3144 1802 873 732 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2865 3012 4264
Size: 54 1425 50 275 18 1035 147 1252
Cuts at: 4264 4398 4993 5230 5796 6701 7113 7200 7903
Size: 134 595 237 566 905 412 87 703
Cuts at: 7903 8792 10421 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1252 1035 905 889 703 595
566 412 275 237 147 134 87 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5796 6701 7903 1537
Size: 275 3984 905 1202 4090
Fragments arranged by size:
4090 3984 1202 905 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2507 2618 2894 6446 8386 1353
Size: 972 182 111 276 3552 1940 3423
Fragments arranged by size:
3552 3423 1940 972 276 182 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9043 9417 9563 387
Size: 1064 138 7454 374 146 1280
Fragments arranged by size:
7454 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3214 3633 5953 7657 3214
Size: 419 2320 1704 6013
Fragments arranged by size:
6013 2320 1704 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2739 2901 3230 3303 3986 4324
Size: 393 488 619 162 329 73 683 338
Cuts at: 4324 5541 5579 5610 5930 5939 6101 6143 6203
Size: 1217 38 31 320 9 162 42 60
Cuts at: 6203 6338 6925 8519 9116 9903 10171 1239
Size: 135 587 1594 597 787 268 1524
Fragments arranged by size:
1594 1524 1217 787 683 619 597 587
488 393 338 329 320 268 162 162
135 73 60 42 38 31 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2996 3866 6524 6940 7673 2465
Size: 531 870 2658 416 733 5248
Fragments arranged by size:
5248 2658 870 733 531 416
EcoO109I rG'GnC_Cy
Cuts at: 2095 3328 3583 3640 4315 4453 4958 6118 6481
Size: 1233 255 57 675 138 505 1160 363
Cuts at: 6481 6482 6979 7665 7666 2095
Size: 1 497 686 1 4885
Fragments arranged by size:
4885 1233 1160 686 675 505 497 363
255 138 57 1 1
EcoRI G'AATT_C
Cuts at: 7765 8143 7765
Size: 378 10078
EcoRV GAT'ATC
Cuts at: 5965 5965
Size: 10456
FspI TGC'GCA
Cuts at: 6427 8962 6427
Size: 2535 7921
HaeII r_GCGC'y
Cuts at: 1678 3178 3257 3547 4074 6736 7191 9603 1678
Size: 1500 79 290 527 2662 455 2412 2531
Fragments arranged by size:
2662 2531 2412 1500 527 455 290 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2327 2359 2675
Size: 32 135 32 527 32 196 32 316
Cuts at: 2675 2707 2717 2749 3276 3308 4683 4715 5535
Size: 32 10 32 527 32 1375 32 820
Cuts at: 5535 5567 5890 5922 6811 6843 7433 7465 9097
Size: 32 323 32 889 32 590 32 1632
Cuts at: 9097 9129 9171 9203 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1632 1375 889 820 590 527 527
323 316 196 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2407 2554 4066 5140 5976 6583
Size: 988 281 469 147 1512 1074 836 607
Cuts at: 6583 7800 8046 669
Size: 1217 246 3079
Fragments arranged by size:
3079 1512 1217 1074 988 836 607 469
281 246 147
HindIII A'AGCT_T
Cuts at: 748 5940 748
Size: 5192 5264
HpaI GTT'AAC
Cuts at: 8046 8046
Size: 10456
KasI G'GCGC_C
Cuts at: 3174 3253 6732 3174
Size: 79 3479 6898
Fragments arranged by size:
6898 3479 79
KpnI G_GTAC'C
Cuts at: 7781 7781
Size: 10456
MfeI C'AATT_G
Cuts at: 6385 8055 6385
Size: 1670 8786
MluI A'CGCG_T
Cuts at: 10332 10332
Size: 10456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 7476 9570 9754 1305
Size: 3354 2817 2094 184 2007
Fragments arranged by size:
3354 2817 2094 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2867 3014 5232 7115 7202
Size: 54 1425 343 1035 147 2218 1883 87
Cuts at: 7202 10
Size: 3264
Fragments arranged by size:
3264 2218 1883 1425 1035 343 147 87
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2510 2627 4919 4979 6818 7265
Size: 648 1140 204 117 2292 60 1839 447
Cuts at: 7265 8414 8773 8932 518
Size: 1149 359 159 2042
Fragments arranged by size:
2292 2042 1839 1149 1140 648 447 359
204 159 117 60
MspA1I CmG'CkG
Cuts at: 1606 2879 3943 3976 4080 4177 4489 4548 6289
Size: 1273 1064 33 104 97 312 59 1741
Cuts at: 6289 6700 7699 7872 8549 9697 9942 1606
Size: 411 999 173 677 1148 245 2120
Fragments arranged by size:
2120 1741 1273 1148 1064 999 677 411
312 245 173 104 97 59 33
NaeI GCC'GGC
Cuts at: 2167 5800 2167
Size: 3633 6823
NarI GG'CG_CC
Cuts at: 3175 3254 6733 3175
Size: 79 3479 6898
Fragments arranged by size:
6898 3479 79
NcoI C'CATG_G
Cuts at: 513 1065 3460 4702 513
Size: 552 2395 1242 6267
Fragments arranged by size:
6267 2395 1242 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10456
NgoMIV G'CCGG_C
Cuts at: 2165 5798 2165
Size: 3633 6823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10456
NotI GC'GGCC_GC
Cuts at: 7903 7903
Size: 10456
NsiI A_TGCA'T
Cuts at: 6825 6825
Size: 10456
NspI r_CATG'y
Cuts at: 1132 1229 2173 3676 7216 7606 7814 9359 1132
Size: 97 944 1503 3540 390 208 1545 2229
Fragments arranged by size:
3540 2229 1545 1503 944 390 208 97
PasI CC'CwG_GG
Cuts at: 1348 3304 4781 1348
Size: 1956 1477 7023
Fragments arranged by size:
7023 1956 1477
PciI A'CATG_T
Cuts at: 3672 7212 9355 3672
Size: 3540 2143 4773
Fragments arranged by size:
4773 3540 2143
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3304 4599 1108
Size: 2196 1295 6965
Fragments arranged by size:
6965 2196 1295
PfoI T'CCnGG_A
Cuts at: 3516 3887 4137 6908 3516
Size: 371 250 2771 7064
Fragments arranged by size:
7064 2771 371 250
PmeI GTTT'AAAC
Cuts at: 7760 7760
Size: 10456
PmlI CAC'GTG
Cuts at: 5713 6109 5713
Size: 396 10060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7148 7180 8525 8557 10038 10070 7148
Size: 32 1345 32 1481 32 7534
Fragments arranged by size:
7534 1481 1345 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3583 3640 4315 4453 4958 3583
Size: 57 675 138 505 9081
Fragments arranged by size:
9081 675 505 138 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10456
PsiI TTA'TAA
Cuts at: 7710 8026 7710
Size: 316 10140
PspOMI G'GGCC_C
Cuts at: 2094 3917 6481 6978 7665 2094
Size: 1823 2564 497 687 4885
Fragments arranged by size:
4885 2564 1823 687 497
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4349 4381 4349
Size: 32 10424
PstI C_TGCA'G
Cuts at: 830 2378 3000 4037 5169 6192 6252 7134 7808
Size: 1548 622 1037 1132 1023 60 882 674
Cuts at: 7808 830
Size: 3478
Fragments arranged by size:
3478 1548 1132 1037 1023 882 674 622
60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10456
PvuII CAG'CTG
Cuts at: 3976 4177 6289 3976
Size: 201 2112 8143
Fragments arranged by size:
8143 2112 201
SacI G_AGCT'C
Cuts at: 3216 3635 5955 7659 3216
Size: 419 2320 1704 6013
Fragments arranged by size:
6013 2320 1704 419
SalI G'TCGA_C
Cuts at: 1936 5138 7798 1936
Size: 3202 2660 4594
Fragments arranged by size:
4594 3202 2660
SanDI GG'GwC_CC
Cuts at: 3583 3640 4315 3583
Size: 57 675 9724
Fragments arranged by size:
9724 675 57
SapI GCTCTTCn'nnn_
Cuts at: 2618 2618
Size: 10456
SbfI CC_TGCA'GG
Cuts at: 3000 6252 7808 3000
Size: 3252 1556 5648
Fragments arranged by size:
5648 3252 1556
ScaI AGT'ACT
Cuts at: 8704 10416 8704
Size: 1712 8744
SfcI C'TryA_G
Cuts at: 826 1045 2374 2996 3179 3394 3971 4033 5165
Size: 219 1329 622 183 215 577 62 1132
Cuts at: 5165 6188 6248 7130 7804 8939 9620 9811 826
Size: 1023 60 882 674 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 1132 1023 882 681 674
622 577 219 215 191 183 62 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 6252 6252
Size: 10456
SfoI GGC'GCC
Cuts at: 3176 3255 6734 3176
Size: 79 3479 6898
Fragments arranged by size:
6898 3479 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10456
SmaI CCC'GGG
Cuts at: 1872 2093 2972 6048 6752 6977 7783 1872
Size: 221 879 3076 704 225 806 4545
Fragments arranged by size:
4545 3076 879 806 704 225 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3494 4232 4511 5859 5879 6281
Size: 197 926 1551 738 279 1348 20 402
Cuts at: 6281 8560 9461 9723 820
Size: 2279 901 262 1553
Fragments arranged by size:
2279 1553 1551 1348 926 901 738 402
279 262 197 20
SnaBI TAC'GTA
Cuts at: 493 7753 8247 493
Size: 7260 494 2702
Fragments arranged by size:
7260 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10456
SphI G_CATG'C
Cuts at: 1132 2173 7814 1132
Size: 1041 5641 3774
Fragments arranged by size:
5641 3774 1041
SspI AAT'ATT
Cuts at: 5 52 4156 8380 5
Size: 47 4104 4224 2081
Fragments arranged by size:
4224 4104 2081 47
StuI AGG'CCT
Cuts at: 1517 3041 3704 1517
Size: 1524 663 8269
Fragments arranged by size:
8269 1524 663
StyI C'CwwG_G
Cuts at: 513 1065 2236 3249 3460 3921 3984 4702 5002
Size: 552 1171 1013 211 461 63 718 300
Cuts at: 5002 5110 5282 5925 6736 7119 7470 7895 513
Size: 108 172 643 811 383 351 425 3074
Fragments arranged by size:
3074 1171 1013 811 718 643 552 461
425 383 351 300 211 172 108 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2131 5157 7300 7486 8483 8500 8653
Size: 1255 551 3026 2143 186 997 17 153
Cuts at: 8653 8838 9283 325
Size: 185 445 1498
Fragments arranged by size:
3026 2143 1498 1255 997 551 445 186
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3067 3339 4911
Size: 274 80 33 51 1443 1090 272 1572
Cuts at: 4911 5175 6555 7209 8702 10414 96
Size: 264 1380 654 1493 1712 138
Fragments arranged by size:
1712 1572 1493 1443 1380 1090 654 274
272 264 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 3227 3259 3406 3438 6713 6745 10416
Size: 32 667 32 147 32 3275 32 3671
Cuts at: 10416 10448 2528
Size: 32 2536
Fragments arranged by size:
3671 3275 2536 667 147 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2659 4663 4897 6358 1316
Size: 1343 2004 234 1461 5414
Fragments arranged by size:
5414 2004 1461 1343 234
XbaI T'CTAG_A
Cuts at: 7792 7792
Size: 10456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3586 3586
Size: 10456
XhoI C'TCGA_G
Cuts at: 1943 5859 1943
Size: 3916 6540
XmaI C'CCGG_G
Cuts at: 1870 2091 2970 6046 6750 6975 7781 1870
Size: 221 879 3076 704 225 806 4545
Fragments arranged by size:
4545 3076 879 806 704 225 221
XmnI GAAnn'nnTTC
Cuts at: 8585 8585
Size: 10456
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3238 6915 8264 276
Size: 53 83 186 936 1704 3677 1349 2468
Fragments arranged by size:
3677 2468 1704 1349 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI FalI FseI FspAI NruI
PacI PspXI RsrII SacII SexAI SrfI SwaI