Restriction map for
pFN21ASDA0457
(Circular) (Six-base) MAPSORT of: pFN21ASDA0457.seq Check: 37 from: 1 to: 7225
pFN21ASDA0457 7225 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 7225
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5035 278
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4568 1937
Size: 2631 4594
Acc65I G'GTAC_C
Cuts at: 4546 4546
Size: 7225
AclI AA'CG_TT
Cuts at: 1563 5352 5725 1563
Size: 3789 373 3063
Fragments arranged by size:
3789 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2803 3077 3086 3383 5288 6672 6940 1632
Size: 1171 274 9 297 1905 1384 268 1917
Fragments arranged by size:
1917 1905 1384 1171 297 274 268 9
AflII C'TTAA_G
Cuts at: 820 1017 3079 4046 820
Size: 197 2062 967 3999
Fragments arranged by size:
3999 2062 967 197
AflIII A'CryG_T
Cuts at: 1203 3369 6124 7101 1203
Size: 2166 2755 977 1327
Fragments arranged by size:
2755 2166 1327 977
AgeI A'CCGG_T
Cuts at: 6056 6056
Size: 7225
AhdI GACnn_n'nnGTC
Cuts at: 5954 5954
Size: 7225
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2420 2478 2722 3605 4501 1184
Size: 1236 58 244 883 896 3908
Fragments arranged by size:
3908 1236 896 883 244 58
AlwNI CAG_nnn'CTG
Cuts at: 2177 4968 6540 2177
Size: 2791 1572 2862
Fragments arranged by size:
2862 2791 1572
ApaLI G'TGCA_C
Cuts at: 5282 6438 5282
Size: 1156 6069
ApoI r'AATT_y
Cuts at: 1493 3728 4534 4760 4912 7021 1493
Size: 2235 806 226 152 2109 1697
Fragments arranged by size:
2235 2109 1697 806 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5779 160
Size: 872 4747 1606
Fragments arranged by size:
4747 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 4550 1870
Size: 73 347 2260 4545
Fragments arranged by size:
4545 2260 347 73
BamHI G'GATC_C
Cuts at: 2938 4555 4922 2938
Size: 1617 367 5241
Fragments arranged by size:
5241 1617 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2396 4546 6001 618
Size: 325 217 627 258 351 2150 1455 1842
Fragments arranged by size:
2150 1842 1455 627 351 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2315 2747 4401 4544 1391
Size: 924 432 1654 143 4072
Fragments arranged by size:
4072 1654 924 432 143
BbeI G_GCGC'C
Cuts at: 2049 2049
Size: 7225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3171 4256 4311 928
Size: 971 1272 1085 55 3842
Fragments arranged by size:
3842 1272 1085 971 55
BbvCI CC'TCA_GC
Cuts at: 3607 3896 3607
Size: 289 6936
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5416 5450 1903
Size: 34 3479 34 3678
Fragments arranged by size:
3678 3479 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5117 6327 5117
Size: 1210 6015
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7225
BfrBI ATG'CAT
Cuts at: 2896 3802 2896
Size: 906 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2413 5836 136
Size: 107 122 71 631 605 741 3423 1525
Fragments arranged by size:
3423 1525 741 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4372 7220 4372
Size: 2848 4377
BlpI GC'TnA_GC
Cuts at: 2417 4642 2417
Size: 2225 5000
Bme1580I G_kGCm'C
Cuts at: 1790 2210 5286 6442 1790
Size: 420 3076 1156 2573
Fragments arranged by size:
3076 2573 1156 420
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4956 5914 442
Size: 416 53 83 399 24 3539 958 1753
Fragments arranged by size:
3539 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2860 2911 3497 3644 3905 4127 4385 4427
Size: 1621 51 586 147 261 222 258 42
Cuts at: 4427 5885 1239
Size: 1458 2579
Fragments arranged by size:
2579 1621 1458 586 261 258 222 147
51 42
Bpu10I CC'TnA_GC
Cuts at: 2980 3011 3354 3607 3896 2980
Size: 31 343 253 289 6309
Fragments arranged by size:
6309 343 289 253 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2629 5350 6215 6513 2629
Size: 2721 865 298 3341
Fragments arranged by size:
3341 2721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 2845 5888 882
Size: 617 978 368 3043 2219
Fragments arranged by size:
3043 2219 978 617 368
BsaAI yAC'GTr
Cuts at: 493 1204 3370 4522 5016 493
Size: 711 2166 1152 494 2702
Fragments arranged by size:
2702 2166 1152 711 494
BsaBI GATnn'nnATC
Cuts at: 4371 7219 4371
Size: 2848 4377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2493 5032 5414
Size: 53 83 186 936 513 447 2539 382
Cuts at: 5414 6049 275
Size: 635 1451
Fragments arranged by size:
2539 1451 936 635 513 447 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2217 2307 3054 3640 5658 6056 6330 6477 6888
Size: 90 747 586 2018 398 274 147 411
Cuts at: 6888 2217
Size: 2554
Fragments arranged by size:
2554 2018 747 586 411 398 274 147
90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2906 2936 1178
Size: 30 165 30 1503 30 5467
Fragments arranged by size:
5467 1503 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2914 3509 3863 3884 4200 1186
Size: 1072 656 595 354 21 316 4211
Fragments arranged by size:
4211 1072 656 595 354 316 21
BseYI C'CCAG_C
Cuts at: 2050 2197 2444 2499 3937 6428 6924 2050
Size: 147 247 55 1438 2491 496 2351
Fragments arranged by size:
2491 2351 1438 496 247 147 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 3176 4081 1163
Size: 573 613 827 905 4307
Fragments arranged by size:
4307 905 827 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4675 5436 6464 1540
Size: 275 186 2674 761 1028 2301
Fragments arranged by size:
2674 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3987 4401 5286 5371 6442 3987
Size: 414 885 85 1071 4770
Fragments arranged by size:
4770 1071 885 414 85
BsmI GAATG_Cn'
Cuts at: 2896 4736 4829 2896
Size: 1840 93 5292
Fragments arranged by size:
5292 1840 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3137 3376 3137
Size: 239 6986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2315 2747 3987 4401 4544 5286
Size: 399 420 105 432 1240 414 143 742
Cuts at: 5286 5371 6442 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1240 1071 742 432 420 414 399
143 105 85
BspEI T'CCGG_A
Cuts at: 2307 6888 2307
Size: 4581 2644
BspHI T'CATG_A
Cuts at: 4363 5114 6844 4363
Size: 751 1730 4744
Fragments arranged by size:
4744 1730 751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 4580 844
Size: 1275 2461 3489
Fragments arranged by size:
3489 2461 1275
BsrBI CCG'CTC
Cuts at: 1394 1823 5112 1394
Size: 429 3289 3507
Fragments arranged by size:
3507 3289 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2734 5720 5894 65
Size: 1183 1486 2986 174 1396
Fragments arranged by size:
2986 1486 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 3611 5869 6056 1700
Size: 110 250 1551 2258 187 2869
Fragments arranged by size:
2869 2258 1551 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7225
BssSI C'ACGA_G
Cuts at: 2789 3379 5285 6297 2789
Size: 590 1906 1012 3717
Fragments arranged by size:
3717 1906 1012 590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2580 794
Size: 1786 5439
BstBI TT'CG_AA
Cuts at: 2007 6029 2007
Size: 4022 3203
BstEII G'GTnAC_C
Cuts at: 1184 3239 1184
Size: 2055 5170
BstXI CCAn_nnnn'nTGG
Cuts at: 2935 2935
Size: 7225
BstYI r'GATC_y
Cuts at: 1972 2938 4372 4555 4922 5307 5324 6776 6862
Size: 966 1434 183 367 385 17 1452 86
Cuts at: 6862 6874 7220 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1434 966 385 367 346 183
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2601 3743 2040
Size: 561 1142 5522
Fragments arranged by size:
5522 1142 561
BtgI C'CryG_G
Cuts at: 513 1065 2469 3274 513
Size: 552 1404 805 4464
Fragments arranged by size:
4464 1404 805 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2532 2815 487
Size: 683 56 1306 283 4897
Fragments arranged by size:
4897 1306 683 283 56
BtsI GCAGTG_nn'
Cuts at: 2208 3348 4076 4741 5535 5555 2208
Size: 1140 728 665 794 20 3878
Fragments arranged by size:
3878 1140 794 728 665 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3497 3532 529
Size: 35 2933 35 4222
Fragments arranged by size:
4222 2933 35 35
DraI TTT'AAA
Cuts at: 4529 4529
Size: 7225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6232 809
Size: 507 4916 1802
Fragments arranged by size:
4916 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2423 4672 5561
Size: 54 1425 50 275 18 593 2249 889
Cuts at: 5561 7190 8
Size: 1629 43
Fragments arranged by size:
2249 1629 1425 889 593 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4672 1537
Size: 275 2860 4090
Fragments arranged by size:
4090 2860 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3967 5155 1353
Size: 2614 1188 3423
Fragments arranged by size:
3423 2614 1188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5812 6186 6332 387
Size: 1064 138 4223 374 146 1280
Fragments arranged by size:
4223 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4399 4399
Size: 7225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2803 2860 2911 3077 3086 3383 3497
Size: 393 1171 57 51 166 9 297 114
Cuts at: 3497 3644 3905 4127 4385 4427 5288 5885 6672
Size: 147 261 222 258 42 861 597 787
Cuts at: 6672 6940 1239
Size: 268 1524
Fragments arranged by size:
1524 1171 861 787 597 393 297 268
261 258 222 166 147 114 57 51
42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3845 3845
Size: 7225
EcoO109I rG'GnC_Cy
Cuts at: 2036 2174 2605 2794 2036
Size: 138 431 189 6467
Fragments arranged by size:
6467 431 189 138
EcoRI G'AATT_C
Cuts at: 4534 4912 4534
Size: 378 6847
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4358 4358
Size: 7225
FspI TGC'GCA
Cuts at: 5731 5731
Size: 7225
HaeII r_GCGC'y
Cuts at: 1678 2049 6372 1678
Size: 371 4323 2531
Fragments arranged by size:
4323 2531 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 2906 2938 2978
Size: 32 135 32 1142 32 160 32 40
Cuts at: 2978 3010 3059 3091 3842 3874 4110 4142 5866
Size: 32 49 32 751 32 236 32 1724
Cuts at: 5866 5898 5940 5972 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1724 1142 751 236 160 135 49
42 40 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3757 4569 4815 669
Size: 988 281 1819 812 246 3079
Fragments arranged by size:
3079 1819 988 812 281 246
HindIII A'AGCT_T
Cuts at: 748 2647 4110 748
Size: 1899 1463 3863
Fragments arranged by size:
3863 1899 1463
HpaI GTT'AAC
Cuts at: 3757 4815 3757
Size: 1058 6167
KasI G'GCGC_C
Cuts at: 2045 2045
Size: 7225
KpnI G_GTAC'C
Cuts at: 4550 4550
Size: 7225
MfeI C'AATT_G
Cuts at: 4824 4824
Size: 7225
MluI A'CGCG_T
Cuts at: 7101 7101
Size: 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3321 6339 6523 1305
Size: 2016 3018 184 2007
Fragments arranged by size:
3018 2016 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5403
Fragments arranged by size:
5403 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4362 5183 5542 5701 518
Size: 648 3196 821 359 159 2042
Fragments arranged by size:
3196 2042 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2499 2580 2976 3297 3478 3553 3568 4065
Size: 893 81 396 321 181 75 15 497
Cuts at: 4065 4496 4641 5318 6466 6711 1606
Size: 431 145 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 677 497 431 396 321
245 181 145 81 75 15
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 7225
NarI GG'CG_CC
Cuts at: 2046 2046
Size: 7225
NcoI C'CATG_G
Cuts at: 513 1065 2469 513
Size: 552 1404 5269
Fragments arranged by size:
5269 1404 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7225
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 7225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7225
NotI GC'GGCC_GC
Cuts at: 4672 4672
Size: 7225
NsiI A_TGCA'T
Cuts at: 2898 3804 2898
Size: 906 6319
NspI r_CATG'y
Cuts at: 1132 1229 2896 3806 4583 6128 1132
Size: 97 1667 910 777 1545 2229
Fragments arranged by size:
2229 1667 1545 910 777 97
PasI CC'CwG_GG
Cuts at: 1348 2435 3559 1348
Size: 1087 1124 5014
Fragments arranged by size:
5014 1124 1087
PciI A'CATG_T
Cuts at: 6124 6124
Size: 7225
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2755 4082 1108
Size: 1647 1327 4251
Fragments arranged by size:
4251 1647 1327
PfoI T'CCnGG_A
Cuts at: 3660 3920 4143 3660
Size: 260 223 6742
Fragments arranged by size:
6742 260 223
PmeI GTTT'AAAC
Cuts at: 4529 4529
Size: 7225
PmlI CAC'GTG
Cuts at: 3370 3370
Size: 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5294 5326 6807 6839 5294
Size: 32 1481 32 5680
Fragments arranged by size:
5680 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2605 2794 2036
Size: 569 189 6467
Fragments arranged by size:
6467 569 189
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7225
PsiI TTA'TAA
Cuts at: 4795 4795
Size: 7225
PstI C_TGCA'G
Cuts at: 830 2116 2516 2585 2848 3573 3737 4285 4425
Size: 1286 400 69 263 725 164 548 140
Cuts at: 4425 4440 4480 4577 830
Size: 15 40 97 3478
Fragments arranged by size:
3478 1286 725 548 400 263 164 140
97 69 40 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7225
PvuII CAG'CTG
Cuts at: 2499 2580 3478 3553 3568 4065 4496 2499
Size: 81 898 75 15 497 431 5228
Fragments arranged by size:
5228 898 497 431 81 75 15
SacI G_AGCT'C
Cuts at: 4401 4401
Size: 7225
SalI G'TCGA_C
Cuts at: 1936 4567 1936
Size: 2631 4594
SbfI CC_TGCA'GG
Cuts at: 4577 4577
Size: 7225
ScaI AGT'ACT
Cuts at: 5473 7185 5473
Size: 1712 5513
SfcI C'TryA_G
Cuts at: 826 1045 2112 2512 2581 2844 3416 3569 3733
Size: 219 1067 400 69 263 572 153 164
Cuts at: 3733 4136 4167 4281 4421 4436 4476 4573 5708
Size: 403 31 114 140 15 40 97 1135
Cuts at: 5708 6389 6580 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1067 681 572 403 400 263
219 191 164 153 140 114 97 69
40 31 15
SfoI GGC'GCC
Cuts at: 2047 2047
Size: 7225
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
SgrAI Cr'CCGG_yG
Cuts at: 1700 2060 1700
Size: 360 6865
SmaI CCC'GGG
Cuts at: 1872 4552 1872
Size: 2680 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2290 2644 3079 4046 5329 6230
Size: 197 926 347 354 435 967 1283 901
Cuts at: 6230 6492 820
Size: 262 1553
Fragments arranged by size:
1553 1283 967 926 901 435 354 347
262 197
SnaBI TAC'GTA
Cuts at: 493 4522 5016 493
Size: 4029 494 2702
Fragments arranged by size:
4029 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7225
SphI G_CATG'C
Cuts at: 1132 3806 4583 1132
Size: 2674 777 3774
Fragments arranged by size:
3774 2674 777
SspI AAT'ATT
Cuts at: 5 52 5149 5
Size: 47 5097 2081
Fragments arranged by size:
5097 2081 47
StuI AGG'CCT
Cuts at: 1517 2298 3587 1517
Size: 781 1289 5155
Fragments arranged by size:
5155 1289 781
StyI C'CwwG_G
Cuts at: 513 1065 2469 4664 513
Size: 552 1404 2195 3074
Fragments arranged by size:
3074 2195 1404 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 3323 5252 5269 5422 5607 6052
Size: 1255 856 887 1929 17 153 185 445
Cuts at: 6052 325
Size: 1498
Fragments arranged by size:
1929 1498 1255 887 856 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4032 4326 5471
Size: 274 80 33 51 1443 2055 294 1145
Cuts at: 5471 7183 96
Size: 1712 138
Fragments arranged by size:
2055 1712 1443 1145 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 7185 7217 2246
Size: 32 4907 32 2254
Fragments arranged by size:
4907 2254 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7225
XbaI T'CTAG_A
Cuts at: 4561 4561
Size: 7225
XhoI C'TCGA_G
Cuts at: 1943 2290 1943
Size: 347 6878
XmaI C'CCGG_G
Cuts at: 1870 4550 1870
Size: 2680 4545
XmnI GAAnn'nnTTC
Cuts at: 5354 5354
Size: 7225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5033 276
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI NruI PacI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI