Restriction map for
pFN21ASDA0427
(Circular) (Six-base) MAPSORT of: pFN21ASDA0427.seq Check: 4298 from: 1 to: 6523
pFN21ASDA0427 6523 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3545 3545
Size: 6523
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3866 1937
Size: 1929 4594
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 4586 5970 6238 1632
Size: 697 2257 1384 268 1917
Fragments arranged by size:
2257 1917 1384 697 268
AfeI AGC'GCT
Cuts at: 2099 3440 2099
Size: 1341 5182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 5422 6399 1203
Size: 4219 977 1327
Fragments arranged by size:
4219 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 2140 2238 2431 3808 4266 5838 2140
Size: 98 193 1377 458 1572 2825
Fragments arranged by size:
2825 1572 1377 458 193 98
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 3832 4058 4210 6319 1493
Size: 2339 226 152 2109 1697
Fragments arranged by size:
2339 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2903 3014 3848 1870
Size: 73 307 653 111 834 4545
Fragments arranged by size:
4545 834 653 307 111 73
BamHI G'GATC_C
Cuts at: 3853 4220 3853
Size: 367 6156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 2772 3479 3844 5299
Size: 325 217 627 564 421 707 365 1455
Cuts at: 5299 618
Size: 1842
Fragments arranged by size:
1842 1455 707 627 564 421 365 325
217
BanII G_rGCy'C
Cuts at: 1391 2263 2714 3507 3552 3660 3741 3842 1391
Size: 872 451 793 45 108 81 101 4072
Fragments arranged by size:
4072 872 793 451 108 101 81 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3048 3466 928
Size: 971 1149 418 3985
Fragments arranged by size:
3985 1149 971 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4714 4748 1903
Size: 34 2777 34 3678
Fragments arranged by size:
3678 2777 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2939 5134 136
Size: 107 122 71 631 605 1267 2195 1525
Fragments arranged by size:
2195 1525 1267 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6518 6518
Size: 6523
BlpI GC'TnA_GC
Cuts at: 2713 3940 2713
Size: 1227 5296
Bme1580I G_kGCm'C
Cuts at: 1790 2312 2895 3527 3692 4584 5740 1790
Size: 522 583 632 165 892 1156 2573
Fragments arranged by size:
2573 1156 892 632 583 522 165
BmgBI CAC'GTC
Cuts at: 1318 3187 3433 1318
Size: 1869 246 4408
Fragments arranged by size:
4408 1869 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 2194 2386
Size: 416 53 83 399 24 762 15 192
Cuts at: 2386 4254 5212 442
Size: 1868 958 1753
Fragments arranged by size:
1868 1753 958 762 416 399 192 83
53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2757
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2141 2888 3035 3647 5183 1239
Size: 875 27 747 147 612 1536 2579
Fragments arranged by size:
2579 1536 875 747 612 147 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4648 5513 5811 4648
Size: 865 298 5360
Fragments arranged by size:
5360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2611 2834 5186 882
Size: 617 1112 223 2352 2219
Fragments arranged by size:
2352 2219 1112 617 223
BsaAI yAC'GTr
Cuts at: 493 1204 2116 3820 4314 493
Size: 711 912 1704 494 2702
Fragments arranged by size:
2702 1704 912 711 494
BsaBI GATnn'nnATC
Cuts at: 3152 6517 3152
Size: 3365 3158
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3767 4330 4712 5347
Size: 53 83 186 936 2234 563 382 635
Cuts at: 5347 275
Size: 1451
Fragments arranged by size:
2234 1451 936 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3366 4956 5354 5628 5775 6186 3366
Size: 1590 398 274 147 411 3703
Fragments arranged by size:
3703 1590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3103 3133 1178
Size: 30 165 30 1700 30 4568
Fragments arranged by size:
4568 1700 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2041 2102 2605 3230 3257 3368 3428
Size: 837 18 61 503 625 27 111 60
Cuts at: 3428 1186
Size: 4281
Fragments arranged by size:
4281 837 625 503 111 61 60 27
18
BseYI C'CCAG_C
Cuts at: 2305 3123 3250 3445 5726 6222 2305
Size: 818 127 195 2281 496 2606
Fragments arranged by size:
2606 2281 818 496 195 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 5533
Fragments arranged by size:
5533 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2446 3295 3716 3973 4734 5762
Size: 275 186 445 849 421 257 761 1028
Cuts at: 5762 1540
Size: 2301
Fragments arranged by size:
2301 1028 849 761 445 421 275 257
186
BsiHKAI G_wGCw'C
Cuts at: 3519 3552 3660 3741 4584 4669 5740 3519
Size: 33 108 81 843 85 1071 4302
Fragments arranged by size:
4302 1071 843 108 85 81 33
BsmI GAATG_Cn'
Cuts at: 4034 4127 4034
Size: 93 6430
BsmBI CGTCTCn'nnnn_
Cuts at: 3104 3104
Size: 6523
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2312 2714 2895 3507 3519 3527
Size: 399 473 49 402 181 612 12 8
Cuts at: 3527 3552 3660 3692 3741 3842 4584 4669 5740
Size: 25 108 32 49 101 742 85 1071
Cuts at: 5740 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 612 473 402 399 181
108 101 85 49 49 32 25 12
8
BspEI T'CCGG_A
Cuts at: 3366 6186 3366
Size: 2820 3703
BspHI T'CATG_A
Cuts at: 4412 6142 4412
Size: 1730 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2373 3545 3878 844
Size: 1529 1172 333 3489
Fragments arranged by size:
3489 1529 1172 333
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 2950 3031 4410 1394
Size: 429 250 877 81 1379 3507
Fragments arranged by size:
3507 1379 877 429 250 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 2836 5018 5192 65
Size: 1183 1588 2182 174 1396
Fragments arranged by size:
2182 1588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2763 3618 5167 5354 1700
Size: 110 953 855 1549 187 2869
Fragments arranged by size:
2869 1549 953 855 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 3518 4583 5595 3518
Size: 1065 1012 4446
Fragments arranged by size:
4446 1065 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 2431 794
Size: 1346 291 4886
Fragments arranged by size:
4886 1346 291
BstBI TT'CG_AA
Cuts at: 2007 5327 2007
Size: 3320 3203
BstEII G'GTnAC_C
Cuts at: 1184 2651 1184
Size: 1467 5056
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 3211 2648
Size: 563 5960
BstYI r'GATC_y
Cuts at: 1972 3153 3853 4220 4605 4622 6074 6160 6172
Size: 1181 700 367 385 17 1452 86 12
Cuts at: 6172 6518 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1181 700 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2728 3542 2728
Size: 814 5709
BtgI C'CryG_G
Cuts at: 513 1065 2022 2746 2776 3105 513
Size: 552 957 724 30 329 3931
Fragments arranged by size:
3931 957 724 552 329 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5784
Fragments arranged by size:
5784 683 56
BtsI GCAGTG_nn'
Cuts at: 2674 2874 4039 4833 4853 2674
Size: 200 1165 794 20 4344
Fragments arranged by size:
4344 1165 794 200 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 3102 3663
Size: 54 1425 50 275 18 495 777 561
Cuts at: 3663 3970 4859 6488 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 889 777 561 495 307 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4453 1353
Size: 3100 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3550 3658 3739 3550
Size: 108 81 6334
Fragments arranged by size:
6334 108 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2141 2329 2888 3035 3647 4586
Size: 393 482 27 188 559 147 612 939
Cuts at: 4586 5183 5970 6238 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 939 787 612 597 559 482 393
268 188 147 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2731 3467 3541 2731
Size: 736 74 5713
Fragments arranged by size:
5713 736 74
EcoO109I rG'GnC_Cy
Cuts at: 2181 2362 2512 2908 2181
Size: 181 150 396 5796
Fragments arranged by size:
5796 396 181 150
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 6523
FspI TGC'GCA
Cuts at: 3266 3488 5029 3266
Size: 222 1541 4760
Fragments arranged by size:
4760 1541 222
FspAI rTGC'GCAy
Cuts at: 3266 3266
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 2101 2770 3341 3442 5670 1678
Size: 423 669 571 101 2228 2531
Fragments arranged by size:
2531 2228 669 571 423 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2584 2616 3103 3135 5164
Size: 32 135 32 1012 32 487 32 2029
Cuts at: 5164 5196 5238 5270 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2029 1012 487 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3867 4113 669
Size: 988 281 1929 246 3079
Fragments arranged by size:
3079 1929 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6523
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 4122 4122
Size: 6523
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2434 3315 5637 5821 1305
Size: 1129 881 2322 184 2007
Fragments arranged by size:
2322 2007 1129 881 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3665 10
Size: 54 1425 343 1833 2868
Fragments arranged by size:
2868 1833 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2829 3209 4481 4840 4999 518
Size: 648 1663 380 1272 359 159 2042
Fragments arranged by size:
2042 1663 1272 648 380 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 2227 2238 3314 3637 3753 3939 4616
Size: 604 17 11 1076 323 116 186 677
Cuts at: 4616 5764 6009 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1076 677 604 323 245 186
116 17 11
NaeI GCC'GGC
Cuts at: 3620 3620
Size: 6523
NcoI C'CATG_G
Cuts at: 513 1065 2022 2746 2776 513
Size: 552 957 724 30 4260
Fragments arranged by size:
4260 957 724 552 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NgoMIV G'CCGG_C
Cuts at: 3618 3618
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2305 3169 3388 3881 5426 1132
Size: 97 1076 864 219 493 1545 2229
Fragments arranged by size:
2229 1545 1076 864 493 219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6523
PciI A'CATG_T
Cuts at: 5422 5422
Size: 6523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 1108
Size: 1446 5077
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2181 2362 2512 2181
Size: 181 150 6192
Fragments arranged by size:
6192 181 150
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6523
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PspXI vC'TCGA_Gb
Cuts at: 2903 2903
Size: 6523
PstI C_TGCA'G
Cuts at: 830 2041 2219 2370 2881 3393 3423 3609 3875
Size: 1211 178 151 511 512 30 186 266
Cuts at: 3875 830
Size: 3478
Fragments arranged by size:
3478 1211 512 511 266 186 178 151
30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2238 3637 3753 2238
Size: 1399 116 5008
Fragments arranged by size:
5008 1399 116
SacI G_AGCT'C
Cuts at: 3552 3660 3741 3552
Size: 108 81 6334
Fragments arranged by size:
6334 108 81
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 3785 4771 6483 3785
Size: 986 1712 3825
Fragments arranged by size:
3825 1712 986
SexAI A'CCwGG_T
Cuts at: 2127 2127
Size: 6523
SfcI C'TryA_G
Cuts at: 826 1045 2037 2215 2366 2752 2877 3389 3419
Size: 219 992 178 151 386 125 512 30
Cuts at: 3419 3605 3871 5006 5687 5878 826
Size: 186 266 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 681 512 386 266 219
191 186 178 151 125 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3850 1872
Size: 1978 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 4627 5528 5790 820
Size: 197 926 960 1724 901 262 1553
Fragments arranged by size:
1724 1553 960 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3820 4314 493
Size: 3327 494 2702
Fragments arranged by size:
3327 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 3169 3881 1132
Size: 2037 712 3774
Fragments arranged by size:
3774 2037 712
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6523
StyI C'CwwG_G
Cuts at: 513 1065 2022 2746 2776 3962 513
Size: 552 957 724 30 1186 3074
Fragments arranged by size:
3074 1186 957 724 552 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2405 2808 4550 4567 4720 4905 5350
Size: 1255 825 403 1742 17 153 185 445
Cuts at: 5350 325
Size: 1498
Fragments arranged by size:
1742 1498 1255 825 445 403 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 3783 4769
Size: 274 80 33 51 1443 1634 172 986
Cuts at: 4769 6481 96
Size: 1712 138
Fragments arranged by size:
1712 1634 1443 986 274 172 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 6483 6515 2681
Size: 32 3770 32 2689
Fragments arranged by size:
3770 2689 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2185 3712 1316
Size: 869 1527 4127
Fragments arranged by size:
4127 1527 869
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 2903 1943
Size: 960 5563
XmaI C'CCGG_G
Cuts at: 1870 3848 1870
Size: 1978 4545
XmnI GAAnn'nnTTC
Cuts at: 2579 3398 4652 2579
Size: 819 1254 4450
Fragments arranged by size:
4450 1254 819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoRV FseI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI