Restriction map for
pFN21ASDA0426
(Circular) (Six-base) MAPSORT of: pFN21ASDA0426.seq Check: 1448 from: 1 to: 6541
pFN21ASDA0426 6541 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2476 3884 1937
Size: 539 1408 4594
Fragments arranged by size:
4594 1408 539
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2152 2643 2730 3358 3691 4604 5988 6256
Size: 520 491 87 628 333 913 1384 268
Cuts at: 6256 1632
Size: 1917
Fragments arranged by size:
1917 1384 913 628 520 491 333 268
87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 5440 6417 1203
Size: 4237 977 1327
Fragments arranged by size:
4237 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 2196 2472 5270 2196
Size: 276 2798 3467
Fragments arranged by size:
3467 2798 276
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 2245 2278 2305 4284 5856 2245
Size: 33 27 1979 1572 2930
Fragments arranged by size:
2930 1979 1572 33 27
ApaLI G'TGCA_C
Cuts at: 3184 4598 5754 3184
Size: 1414 1156 3971
Fragments arranged by size:
3971 1414 1156
ApoI r'AATT_y
Cuts at: 1493 2602 2657 3405 3489 3850 4076 4228 6337
Size: 1109 55 748 84 361 226 152 2109
Cuts at: 6337 1493
Size: 1697
Fragments arranged by size:
2109 1697 1109 748 361 226 152 84
55
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 3866 1870
Size: 73 1923 4545
Fragments arranged by size:
4545 1923 73
BamHI G'GATC_C
Cuts at: 2904 3871 4238 2904
Size: 967 367 5207
Fragments arranged by size:
5207 967 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3251 3862 5317 618
Size: 325 217 627 1464 611 1455 1842
Fragments arranged by size:
1842 1464 1455 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2328 2730 3794 3860 1391
Size: 937 402 1064 66 4072
Fragments arranged by size:
4072 1064 937 402 66
BbeI G_GCGC'C
Cuts at: 3255 3255
Size: 6541
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 2651 3728 928
Size: 971 247 505 1077 3741
Fragments arranged by size:
3741 1077 971 505 247
BbvCI CC'TCA_GC
Cuts at: 2246 2246
Size: 6541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2214 2248 4732 4766 1903
Size: 34 277 34 2484 34 3678
Fragments arranged by size:
3678 2484 277 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4433 5643 4433
Size: 1210 5331
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5152 136
Size: 107 122 71 631 605 3480 1525
Fragments arranged by size:
3480 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3829 6536 3829
Size: 2707 3834
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 3188 4602 5758 1790
Size: 1398 1414 1156 2573
Fragments arranged by size:
2573 1414 1398 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2448 2490 3423
Size: 416 53 83 399 24 1031 42 933
Cuts at: 3423 3675 4272 5230 442
Size: 252 597 958 1753
Fragments arranged by size:
1753 1031 958 933 597 416 399 252
83 53 42 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 6541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2312 2315 2405 2492 2526 2725 2934
Size: 1061 12 3 90 87 34 199 209
Cuts at: 2934 3018 3519 3603 3792 5201 1239
Size: 84 501 84 189 1409 2579
Fragments arranged by size:
2579 1409 1061 501 209 199 189 90
87 84 84 34 12 3
Bpu10I CC'TnA_GC
Cuts at: 2246 3788 2246
Size: 1542 4999
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4666 5531 5829 4666
Size: 865 298 5378
Fragments arranged by size:
5378 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2249 2431 5204 882
Size: 617 750 182 2773 2219
Fragments arranged by size:
2773 2219 750 617 182
BsaAI yAC'GTr
Cuts at: 493 1204 3838 4332 493
Size: 711 2634 494 2702
Fragments arranged by size:
2702 2634 711 494
BsaBI GATnn'nnATC
Cuts at: 6535 6535
Size: 6541
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3252 4348 4730 5365
Size: 53 83 186 936 1719 1096 382 635
Cuts at: 5365 275
Size: 1451
Fragments arranged by size:
1719 1451 1096 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4974 5372 5646 5793 6204 4974
Size: 398 274 147 411 5311
Fragments arranged by size:
5311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2409 2439 1178
Size: 30 165 30 1006 30 5280
Fragments arranged by size:
5280 1006 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2381 1186
Size: 1150 45 5346
Fragments arranged by size:
5346 1150 45
BseYI C'CCAG_C
Cuts at: 2523 5744 6240 2523
Size: 3221 496 2824
Fragments arranged by size:
3221 2824 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 1163
Size: 573 624 5344
Fragments arranged by size:
5344 624 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3991 4752 5780 1540
Size: 275 186 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 3188 3794 4602 4687 5758 2328
Size: 860 606 808 85 1071 3111
Fragments arranged by size:
3111 1071 860 808 606 85
BsmI GAATG_Cn'
Cuts at: 3622 4052 4145 3622
Size: 430 93 6018
Fragments arranged by size:
6018 430 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 2459
Size: 6541
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2730 3188 3794 3860 4602 4687
Size: 399 538 402 458 606 66 742 85
Cuts at: 4687 5758 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 606 538 458 402 399
85 66
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 4430 6160 4430
Size: 1730 4811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2413 3896 844
Size: 1286 283 1483 3489
Fragments arranged by size:
3489 1483 1286 283
BsrBI CCG'CTC
Cuts at: 1394 1823 4428 1394
Size: 429 2605 3507
Fragments arranged by size:
3507 2605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5036 5210 65
Size: 1183 3788 174 1396
Fragments arranged by size:
3788 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3042 3248 5185 5372 1700
Size: 110 1232 206 1937 187 2869
Fragments arranged by size:
2869 1937 1232 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 2700 4601 5613 2700
Size: 1901 1012 3628
Fragments arranged by size:
3628 1901 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6541
BstBI TT'CG_AA
Cuts at: 2007 5345 2007
Size: 3338 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 2904 3156 3829 3871 4238 4623 4640 6092
Size: 932 252 673 42 367 385 17 1452
Cuts at: 6092 6178 6190 6536 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 932 673 385 367 346 252
86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2319 2319
Size: 6541
BtgI C'CryG_G
Cuts at: 513 1065 2022 2956 513
Size: 552 957 934 4098
Fragments arranged by size:
4098 957 934 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 4057 4851 4871 4057
Size: 794 20 5727
Fragments arranged by size:
5727 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3259 3294 529
Size: 35 2695 35 3776
Fragments arranged by size:
3776 2695 35 35
DraI TTT'AAA
Cuts at: 2607 3845 2607
Size: 1238 5303
DraIII CAC_nnn'GTG
Cuts at: 3192 3192
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3040 3988 4877
Size: 54 1425 50 275 18 1210 948 889
Cuts at: 4877 6506 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1210 948 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 2718 4471 1353
Size: 732 633 1753 3423
Fragments arranged by size:
3423 1753 732 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5128 5502 5648 387
Size: 1064 138 3539 374 146 1280
Fragments arranged by size:
3539 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2326 3792 2326
Size: 1466 5075
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2300 2312 2315 2405 2492 2526
Size: 393 520 148 12 3 90 87 34
Cuts at: 2526 2643 2725 2730 2934 3018 3358 3519 3603
Size: 117 82 5 204 84 340 161 84
Cuts at: 3603 3691 3792 4604 5201 5988 6256 1239
Size: 88 101 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 597 520 393 340 268
204 161 148 117 101 90 88 87
84 84 82 34 12 5 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 2318
Size: 6541
EcoO109I rG'GnC_Cy
Cuts at: 2119 3591 2119
Size: 1472 5069
EcoRI G'AATT_C
Cuts at: 3405 3489 3850 4228 3405
Size: 84 361 378 5718
Fragments arranged by size:
5718 378 361 84
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 3255 5688 1678
Size: 1577 2433 2531
Fragments arranged by size:
2531 2433 1577
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2409 2441 2458
Size: 32 135 32 610 32 195 32 17
Cuts at: 2458 2490 2809 2841 3540 3572 5182 5214 5256
Size: 32 319 32 699 32 1610 32 42
Cuts at: 5256 5288 1373
Size: 32 2626
Fragments arranged by size:
2626 1610 699 610 319 195 135 42
32 32 32 32 32 32 32 32
32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 3885 4131 669
Size: 988 281 1947 246 3079
Fragments arranged by size:
3079 1947 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2865 2949 748
Size: 2117 84 4340
Fragments arranged by size:
4340 2117 84
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KasI G'GCGC_C
Cuts at: 3251 3251
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5655 5839 1305
Size: 4350 184 2007
Fragments arranged by size:
4350 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4719
Fragments arranged by size:
4719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3102 4499 4858 5017 518
Size: 648 1936 1397 359 159 2042
Fragments arranged by size:
2042 1936 1397 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3957 4634 5782 6027 1606
Size: 2351 677 1148 245 2120
Fragments arranged by size:
2351 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3252 3252
Size: 6541
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5032
Fragments arranged by size:
5032 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 3899 5444 1132
Size: 97 2670 1545 2229
Fragments arranged by size:
2670 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 5440 5440
Size: 6541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 5289
PfoI T'CCnGG_A
Cuts at: 2827 2827
Size: 6541
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2119
Size: 6541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 3297 4111 3297
Size: 814 5727
PstI C_TGCA'G
Cuts at: 830 3893 830
Size: 3063 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
SacI G_AGCT'C
Cuts at: 2328 3794 2328
Size: 1466 5075
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SapI GCTCTTCn'nnn_
Cuts at: 2718 2718
Size: 6541
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 2165 4789 6501 2165
Size: 2624 1712 2205
Fragments arranged by size:
2624 2205 1712
SfcI C'TryA_G
Cuts at: 826 1045 3889 5024 5705 5896 826
Size: 219 2844 1135 681 191 1471
Fragments arranged by size:
2844 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3253 3253
Size: 6541
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4645 5546 5808 820
Size: 197 926 2702 901 262 1553
Fragments arranged by size:
2702 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3838 4332 493
Size: 3345 494 2702
Fragments arranged by size:
3345 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 2333 3138 1517
Size: 816 805 4920
Fragments arranged by size:
4920 816 805
StyI C'CwwG_G
Cuts at: 513 1065 2022 3980 513
Size: 552 957 1958 3074
Fragments arranged by size:
3074 1958 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 2326 4568 4585 4738 4923 5368
Size: 1255 573 173 2242 17 153 185 445
Cuts at: 5368 325
Size: 1498
Fragments arranged by size:
2242 1498 1255 573 445 185 173 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 4787 6499
Size: 274 80 33 51 1443 186 2624 1712
Cuts at: 6499 96
Size: 138
Fragments arranged by size:
2624 1712 1443 274 186 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 3877 3877
Size: 6541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2192
Size: 6541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 2274 3371 3620 3741 4670 2274
Size: 1097 249 121 929 4145
Fragments arranged by size:
4145 1097 929 249 121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI