Restriction map for
pFN21ASDA0420
(Circular) (Six-base) MAPSORT of: pFN21ASDA0420.seq Check: 2960 from: 1 to: 6817
pFN21ASDA0420 6817 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2231 2231
Size: 6817
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4627 278
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2055 3852 4160 1937
Size: 118 1797 308 4594
Fragments arranged by size:
4594 1797 308 118
Acc65I G'GTAC_C
Cuts at: 2543 4138 2543
Size: 1595 5222
AclI AA'CG_TT
Cuts at: 1563 4944 5317 1563
Size: 3381 373 3063
Fragments arranged by size:
3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2909 3982 4880 6264 6532 1632
Size: 1277 1073 898 1384 268 1917
Fragments arranged by size:
1917 1384 1277 1073 898 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6620
AflIII A'CryG_T
Cuts at: 1203 5716 6693 1203
Size: 4513 977 1327
Fragments arranged by size:
4513 1327 977
AgeI A'CCGG_T
Cuts at: 5648 5648
Size: 6817
AhdI GACnn_n'nnGTC
Cuts at: 2425 3270 5546 2425
Size: 845 2276 3696
Fragments arranged by size:
3696 2276 845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2958 1184
Size: 1774 5043
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 6785
AlwNI CAG_nnn'CTG
Cuts at: 3156 4560 6132 3156
Size: 1404 1572 3841
Fragments arranged by size:
3841 1572 1404
ApaI G_GGCC'C
Cuts at: 2646 2664 3951 2646
Size: 18 1287 5512
Fragments arranged by size:
5512 1287 18
ApaLI G'TGCA_C
Cuts at: 2177 4874 6030 2177
Size: 2697 1156 2964
Fragments arranged by size:
2964 2697 1156
ApoI r'AATT_y
Cuts at: 1493 4126 4352 4504 6613 1493
Size: 2633 226 152 2109 1697
Fragments arranged by size:
2633 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5371 160
Size: 872 4339 1606
Fragments arranged by size:
4339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
AvaI C'yCGr_G
Cuts at: 1870 1943 2865 3297 3708 3907 3944 4142 1870
Size: 73 922 432 411 199 37 198 4545
Fragments arranged by size:
4545 922 432 411 199 198 73 37
BamHI G'GATC_C
Cuts at: 4147 4514 4147
Size: 367 6450
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2721 3195 3691 3704
Size: 325 217 627 756 178 474 496 13
Cuts at: 3704 4138 5593 618
Size: 434 1455 1842
Fragments arranged by size:
1842 1455 756 627 496 474 434 325
217 178 13
BanII G_rGCy'C
Cuts at: 1391 2532 2646 2664 2842 3295 3586 3827 3951
Size: 1141 114 18 178 453 291 241 124
Cuts at: 3951 4007 4136 1391
Size: 56 129 4072
Fragments arranged by size:
4072 1141 453 291 241 178 129 124
114 56 18
BbeI G_GCGC'C
Cuts at: 3695 3708 3695
Size: 13 6804
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 2251 2391 3295 928
Size: 971 220 132 140 904 4450
Fragments arranged by size:
4450 971 904 220 140 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2390 2424 2807 2841 5008 5042 1903
Size: 34 453 34 383 34 2167 34 3678
Fragments arranged by size:
3678 2167 453 383 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4709 5919 4709
Size: 1210 5607
BclI T'GATC_A
Cuts at: 1548 3324 1548
Size: 1776 5041
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5428 136
Size: 107 122 71 631 605 3756 1525
Fragments arranged by size:
3756 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6812 6812
Size: 6817
BlpI GC'TnA_GC
Cuts at: 4049 4234 4049
Size: 185 6632
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2181 2646 2664 3951 4878 6034 1790
Size: 324 67 465 18 1287 927 1156 2573
Fragments arranged by size:
2573 1287 1156 927 465 324 67 18
BmgBI CAC'GTC
Cuts at: 1318 3661 1318
Size: 2343 4474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 2570 4548
Size: 416 53 83 399 24 700 453 1978
Cuts at: 4548 5506 442
Size: 958 1753
Fragments arranged by size:
1978 1753 958 700 453 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6817
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 6817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 2690 2972 3197 3845 4090 5477 1239
Size: 1412 39 282 225 648 245 1387 2579
Fragments arranged by size:
2579 1412 1387 648 282 245 225 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2908 4942 5807 6105 2908
Size: 2034 865 298 3620
Fragments arranged by size:
3620 2034 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 2780 3257 3545 4108 5480 882
Size: 617 816 465 477 288 563 1372 2219
Fragments arranged by size:
2219 1372 816 617 563 477 465 288
BsaAI yAC'GTr
Cuts at: 493 1204 2109 2182 2254 4114 4608 493
Size: 711 905 73 72 1860 494 2702
Fragments arranged by size:
2702 1860 905 711 494 73 72
BsaBI GATnn'nnATC
Cuts at: 6811 6811
Size: 6817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 2567 3692 3705
Size: 53 83 186 936 681 353 1125 13
Cuts at: 3705 3849 4624 5006 5641 275
Size: 144 775 382 635 1451
Fragments arranged by size:
1451 1125 936 775 681 635 382 353
186 144 83 53 13
BsaWI w'CCGG_w
Cuts at: 5250 5648 5922 6069 6480 5250
Size: 398 274 147 411 5587
Fragments arranged by size:
5587 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 3390 3420 3441
Size: 30 165 30 1206 30 751 30 21
Cuts at: 3441 3468 3471 3498 3609 3639 1178
Size: 27 3 27 111 30 4356
Fragments arranged by size:
4356 1206 751 165 111 30 30 30
30 30 27 27 21 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2600 2909 2969 3077 3527 4060 4063
Size: 1105 309 309 60 108 450 533 3
Cuts at: 4063 1186
Size: 3940
Fragments arranged by size:
3940 1105 533 450 309 309 108 60
3
BseYI C'CCAG_C
Cuts at: 2621 3136 3700 3753 3884 4044 6020 6516 2621
Size: 515 564 53 131 160 1976 496 2922
Fragments arranged by size:
2922 1976 564 515 496 160 131 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 3920 4012 1163
Size: 573 549 1635 92 3968
Fragments arranged by size:
3968 1635 573 549 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3209 4267 5028 6056 1540
Size: 275 186 1208 1058 761 1028 2301
Fragments arranged by size:
2301 1208 1058 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2253 2355 2532 2820 3315 3525 3576 3984
Size: 72 102 177 288 495 210 51 408
Cuts at: 3984 4878 4963 6034 2181
Size: 894 85 1071 2964
Fragments arranged by size:
2964 1071 894 495 408 288 210 177
102 85 72 51
BsmI GAATG_Cn'
Cuts at: 4328 4421 4328
Size: 93 6724
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2181 2253 2355 2532 2646 2664
Size: 399 324 67 72 102 177 114 18
Cuts at: 2664 2820 2842 3295 3315 3525 3576 3586 3827
Size: 156 22 453 20 210 51 10 241
Cuts at: 3827 3951 3984 4007 4136 4878 4963 6034 1391
Size: 124 33 23 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 324 241 210
177 156 129 124 114 102 85 72
67 51 33 23 22 20 18 10
BspEI T'CCGG_A
Cuts at: 6480 6480
Size: 6817
BspHI T'CATG_A
Cuts at: 2842 4706 6436 2842
Size: 1864 1730 3223
Fragments arranged by size:
3223 1864 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 3170 3864 4172 844
Size: 1387 939 694 308 3489
Fragments arranged by size:
3489 1387 939 694 308
BsrBI CCG'CTC
Cuts at: 1394 1823 2191 2666 4704 1394
Size: 429 368 475 2038 3507
Fragments arranged by size:
3507 2038 475 429 368
BsrDI GCAATG_nn'
Cuts at: 65 1248 5312 5486 65
Size: 1183 4064 174 1396
Fragments arranged by size:
4064 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 5461 5648 1700
Size: 110 389 3262 187 2869
Fragments arranged by size:
3262 2869 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6817
BssSI C'ACGA_G
Cuts at: 3590 4877 5889 3590
Size: 1287 1012 4518
Fragments arranged by size:
4518 1287 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6817
BstBI TT'CG_AA
Cuts at: 2007 5621 2007
Size: 3614 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6817
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2629 3892 2338
Size: 291 1263 5263
Fragments arranged by size:
5263 1263 291
BstYI r'GATC_y
Cuts at: 1972 2118 2914 3606 4147 4514 4899 4916 6368
Size: 146 796 692 541 367 385 17 1452
Cuts at: 6368 6454 6466 6812 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 796 692 541 385 367 346
146 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2219 2638 3054 3577 3600 513
Size: 552 957 197 419 416 523 23 3730
Fragments arranged by size:
3730 957 552 523 419 416 197 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6078
Fragments arranged by size:
6078 683 56
BtsI GCAGTG_nn'
Cuts at: 2353 3548 4333 5127 5147 2353
Size: 1195 785 794 20 4023
Fragments arranged by size:
4023 1195 794 785 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6782
DraI TTT'AAA
Cuts at: 4121 4121
Size: 6817
DraIII CAC_nnn'GTG
Cuts at: 2185 2911 3595 2185
Size: 726 684 5407
Fragments arranged by size:
5407 726 684
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5824 809
Size: 507 4508 1802
Fragments arranged by size:
4508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2995 3206 3597
Size: 54 1425 50 275 18 1165 211 391
Cuts at: 3597 4078 4264 5153 6782 8
Size: 481 186 889 1629 43
Fragments arranged by size:
1629 1425 1165 889 481 391 275 211
186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3206 4264 1537
Size: 275 1394 1058 4090
Fragments arranged by size:
4090 1394 1058 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3504 4747 1353
Size: 2151 1243 3423
Fragments arranged by size:
3423 2151 1243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3005 3370 5404 5778 5924 387
Size: 1064 138 1416 365 2034 374 146 1280
Fragments arranged by size:
2034 1416 1280 1064 374 365 146 138
EcoICRI GAG'CTC
Cuts at: 2530 2530
Size: 6817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 2690 2909 2972 3197 3845 3982
Size: 393 1019 39 219 63 225 648 137
Cuts at: 3982 4090 4880 5477 6264 6532 1239
Size: 108 790 597 787 268 1524
Fragments arranged by size:
1524 1019 790 787 648 597 393 268
225 219 137 108 63 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2858 2951 2858
Size: 93 6724
EcoO109I rG'GnC_Cy
Cuts at: 2617 2643 2660 3220 3392 3757 2617
Size: 26 17 560 172 365 5677
Fragments arranged by size:
5677 560 365 172 26 17
EcoRI G'AATT_C
Cuts at: 4126 4504 4126
Size: 378 6439
FspI TGC'GCA
Cuts at: 5323 5323
Size: 6817
HaeII r_GCGC'y
Cuts at: 1678 3071 3695 3708 4056 5964 1678
Size: 1393 624 13 348 1908 2531
Fragments arranged by size:
2531 1908 1393 624 348 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2609 2641 3256
Size: 32 135 32 839 32 166 32 615
Cuts at: 3256 3288 3414 3446 3468 3500 3609 3641 5458
Size: 32 126 32 22 32 109 32 1817
Cuts at: 5458 5490 5532 5564 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1817 839 615 166 135 126 109
42 32 32 32 32 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3094 4161 4407 669
Size: 988 281 1156 1067 246 3079
Fragments arranged by size:
3079 1156 1067 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6817
HpaI GTT'AAC
Cuts at: 4407 4407
Size: 6817
KasI G'GCGC_C
Cuts at: 3691 3704 3691
Size: 13 6804
KpnI G_GTAC'C
Cuts at: 2547 4142 2547
Size: 1595 5222
MfeI C'AATT_G
Cuts at: 4416 4416
Size: 6817
MluI A'CGCG_T
Cuts at: 6693 6693
Size: 6817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5931 6115 1305
Size: 4626 184 2007
Fragments arranged by size:
4626 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4995
Fragments arranged by size:
4995 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2741 4775 5134 5293 518
Size: 648 1575 2034 359 159 2042
Fragments arranged by size:
2042 2034 1575 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 3136 3535 3579 3732 3739 4035 4233
Size: 615 915 399 44 153 7 296 198
Cuts at: 4233 4910 6058 6303 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 615 399 296 245
198 153 44 7
NaeI GCC'GGC
Cuts at: 2201 2201
Size: 6817
NarI GG'CG_CC
Cuts at: 3692 3705 3692
Size: 13 6804
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5308
Fragments arranged by size:
5308 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6817
NgoMIV G'CCGG_C
Cuts at: 2199 2199
Size: 6817
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6817
NotI GC'GGCC_GC
Cuts at: 4264 4264
Size: 6817
NspI r_CATG'y
Cuts at: 1132 1229 3083 3193 4175 5720 1132
Size: 97 1854 110 982 1545 2229
Fragments arranged by size:
2229 1854 1545 982 110 97
PasI CC'CwG_GG
Cuts at: 1348 2539 2656 3268 1348
Size: 1191 117 612 4897
Fragments arranged by size:
4897 1191 612 117
PciI A'CATG_T
Cuts at: 5716 5716
Size: 6817
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3595 1108
Size: 2487 4330
PfoI T'CCnGG_A
Cuts at: 3155 3609 3155
Size: 454 6363
PmeI GTTT'AAAC
Cuts at: 4121 4121
Size: 6817
PmlI CAC'GTG
Cuts at: 2109 2182 2254 2109
Size: 73 72 6672
Fragments arranged by size:
6672 73 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4886 4918 6399 6431 4886
Size: 32 1481 32 5272
Fragments arranged by size:
5272 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2617 3392 3757 2617
Size: 775 365 5677
Fragments arranged by size:
5677 775 365
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6817
PsiI TTA'TAA
Cuts at: 4387 4387
Size: 6817
PspOMI G'GGCC_C
Cuts at: 2642 2660 3947 2642
Size: 18 1287 5512
Fragments arranged by size:
5512 1287 18
PstI C_TGCA'G
Cuts at: 830 2360 3902 4169 830
Size: 1530 1542 267 3478
Fragments arranged by size:
3478 1542 1530 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6817
PvuII CAG'CTG
Cuts at: 3136 3535 3739 3136
Size: 399 204 6214
Fragments arranged by size:
6214 399 204
SacI G_AGCT'C
Cuts at: 2532 2532
Size: 6817
SacII CC_GC'GG
Cuts at: 2222 3580 2222
Size: 1358 5459
SalI G'TCGA_C
Cuts at: 1936 4159 1936
Size: 2223 4594
SanDI GG'GwC_CC
Cuts at: 2617 3392 2617
Size: 775 6042
SbfI CC_TGCA'GG
Cuts at: 4169 4169
Size: 6817
ScaI AGT'ACT
Cuts at: 5065 6777 5065
Size: 1712 5105
SexAI A'CCwGG_T
Cuts at: 2907 2907
Size: 6817
SfcI C'TryA_G
Cuts at: 826 1045 2356 3370 3898 4165 5300 5981 6172
Size: 219 1311 1014 528 267 1135 681 191
Cuts at: 6172 826
Size: 1471
Fragments arranged by size:
1471 1311 1135 1014 681 528 267 219
191
SfoI GGC'GCC
Cuts at: 3693 3706 3693
Size: 13 6804
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6817
SmaI CCC'GGG
Cuts at: 1872 2867 3710 3909 3946 4144 1872
Size: 995 843 199 37 198 4545
Fragments arranged by size:
4545 995 843 199 198 37
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2887 4921 5822 6084 820
Size: 197 926 944 2034 901 262 1553
Fragments arranged by size:
2034 1553 944 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4114 4608 493
Size: 3621 494 2702
Fragments arranged by size:
3621 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6817
SphI G_CATG'C
Cuts at: 1132 4175 1132
Size: 3043 3774
SspI AAT'ATT
Cuts at: 5 52 4741 5
Size: 47 4689 2081
Fragments arranged by size:
4689 2081 47
StuI AGG'CCT
Cuts at: 1517 3402 1517
Size: 1885 4932
StyI C'CwwG_G
Cuts at: 513 1065 2022 2451 3442 4256 513
Size: 552 957 429 991 814 3074
Fragments arranged by size:
3074 991 957 814 552 429
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2415 2626 3613 4844 4861 5014 5199
Size: 1255 835 211 987 1231 17 153 185
Cuts at: 5199 5644 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1231 987 835 445 211 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 5063 6775
Size: 274 80 33 51 1443 498 2588 1712
Cuts at: 6775 96
Size: 138
Fragments arranged by size:
2588 1712 1443 498 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3390 3422 3441 3473 3521 3553 6777 6809 3390
Size: 32 19 32 48 32 3224 32 3398
Fragments arranged by size:
3398 3224 48 32 32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 1316 3920 1316
Size: 2604 4213
XbaI T'CTAG_A
Cuts at: 4153 4153
Size: 6817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2990 4015 2338
Size: 652 1025 5140
Fragments arranged by size:
5140 1025 652
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6817
XmaI C'CCGG_G
Cuts at: 1870 2865 3708 3907 3944 4142 1870
Size: 995 843 199 37 198 4545
Fragments arranged by size:
4545 995 843 199 198 37
XmnI GAAnn'nnTTC
Cuts at: 2099 4946 2099
Size: 2847 3970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4625 276
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI
SwaI