Restriction map for
pFN21ASDA0414
(Circular) (Six-base) MAPSORT of: pFN21ASDA0414.seq Check: 7931 from: 1 to: 6130
pFN21ASDA0414 6130 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2953 2953
Size: 6130
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2127 2299 3940 278
Size: 53 83 186 936 591 172 1641 2468
Fragments arranged by size:
2468 1641 936 591 186 172 83 53
AccI GT'mk_AC
Cuts at: 1937 2370 3473 1937
Size: 433 1103 4594
Fragments arranged by size:
4594 1103 433
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 4193 5577 5845 1632
Size: 771 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 771 268
AfeI AGC'GCT
Cuts at: 2771 2771
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 2443 2575 4859 2443
Size: 132 2284 3714
Fragments arranged by size:
3714 2284 132
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2816 1184
Size: 1632 4498
AlwNI CAG_nnn'CTG
Cuts at: 2089 3128 3873 5445 2089
Size: 1039 745 1572 2774
Fragments arranged by size:
2774 1572 1039 745
ApaLI G'TGCA_C
Cuts at: 2801 4187 5343 2801
Size: 1386 1156 3588
Fragments arranged by size:
3588 1386 1156
ApoI r'AATT_y
Cuts at: 1493 2054 3245 3282 3439 3665 3817 5926 1493
Size: 561 1191 37 157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1191 561 226 157 152 37
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 3455 1870
Size: 73 822 690 4545
Fragments arranged by size:
4545 822 690 73
BamHI G'GATC_C
Cuts at: 2751 3460 3827 2751
Size: 709 367 5054
Fragments arranged by size:
5054 709 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 3451 4906 618
Size: 325 217 627 888 776 1455 1842
Fragments arranged by size:
1842 1455 888 776 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2498 3449 1391
Size: 1107 951 4072
Fragments arranged by size:
4072 1107 951
BbeI G_GCGC'C
Cuts at: 2679 2679
Size: 6130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3203 928
Size: 971 1304 3855
Fragments arranged by size:
3855 1304 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3187 3221 4321 4355 1903
Size: 34 1250 34 1100 34 3678
Fragments arranged by size:
3678 1250 1100 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3150 4022 5232 3150
Size: 872 1210 4048
Fragments arranged by size:
4048 1210 872
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3399 3547 3399
Size: 148 5982
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2805 3033 4191 5347 1790
Size: 371 644 228 1158 1156 2573
Fragments arranged by size:
2573 1158 1156 644 371 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 2336 4790 1239
Size: 1059 38 2454 2579
Fragments arranged by size:
2579 2454 1059 38
Bpu10I CC'TnA_GC
Cuts at: 2639 2639
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4255 5120 5418 4255
Size: 865 298 4967
Fragments arranged by size:
4967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2758 3427 3921 493
Size: 711 1554 669 494 2702
Fragments arranged by size:
2702 1554 711 669 494
BsaBI GATnn'nnATC
Cuts at: 3018 6124 3018
Size: 3106 3024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2296 2676 3937
Size: 53 83 186 936 591 172 380 1261
Cuts at: 3937 4319 4954 275
Size: 382 635 1451
Fragments arranged by size:
1451 1261 936 635 591 382 380 186
172 83 53
BsaWI w'CCGG_w
Cuts at: 2999 4563 4961 5235 5382 5793 2999
Size: 1564 398 274 147 411 3336
Fragments arranged by size:
3336 1564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3331 3361 1178
Size: 30 165 30 1928 30 3947
Fragments arranged by size:
3947 1928 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 2684 2900 1186
Size: 1384 114 216 4416
Fragments arranged by size:
4416 1384 216 114
BseYI C'CCAG_C
Cuts at: 2491 2583 2609 2726 5333 5829 2491
Size: 92 26 117 2607 496 2792
Fragments arranged by size:
2792 2607 496 117 92 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2870 2888 1163
Size: 573 1134 18 4405
Fragments arranged by size:
4405 1134 573 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2564 2598 2645 2805 2823 2862 4191 4276 5347
Size: 34 47 160 18 39 1329 85 1071
Cuts at: 5347 2564
Size: 3347
Fragments arranged by size:
3347 1329 1071 160 85 47 39 34
18
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
BsmBI CGTCTCn'nnnn_
Cuts at: 2131 2131
Size: 6130
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2498 2564 2598 2645 2805 2823
Size: 399 371 337 66 34 47 160 18
Cuts at: 2823 2862 3033 3449 4191 4276 5347 1391
Size: 39 171 416 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 416 399 371 337 171
160 85 66 47 39 34 18
BspEI T'CCGG_A
Cuts at: 2999 5793 2999
Size: 2794 3336
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2613 2953 3047 3485 844
Size: 1769 340 94 438 3489
Fragments arranged by size:
3489 1769 438 340 94
BsrBI CCG'CTC
Cuts at: 1394 1823 2312 4017 1394
Size: 429 489 1705 3507
Fragments arranged by size:
3507 1705 489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3354 4774 4961 1700
Size: 110 1544 1420 187 2869
Fragments arranged by size:
2869 1544 1420 187 110
BsrGI T'GTAC_A
Cuts at: 96 2715 96
Size: 2619 3511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 3266 4190 5202 3266
Size: 924 1012 4194
Fragments arranged by size:
4194 1012 924
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2866 794
Size: 2072 4058
BstBI TT'CG_AA
Cuts at: 2007 4934 2007
Size: 2927 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2734
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2751 3460 3827 4212 4229 5681 5767 5779
Size: 779 709 367 385 17 1452 86 12
Cuts at: 5779 6125 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 779 709 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2985 513
Size: 552 957 963 3658
Fragments arranged by size:
3658 963 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3646 4440 4460 2440
Size: 1206 794 20 4110
Fragments arranged by size:
4110 1206 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 2866 2866
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095
Size: 54 1425 50 275 18 1747 889 1629
Cuts at: 6095 8
Size: 43
Fragments arranged by size:
1747 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 2985 2986 4060 1353
Size: 1194 438 1 1074 3423
Fragments arranged by size:
3423 1194 1074 438 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2336 2403 4193 4790 5577 5845
Size: 393 666 38 67 1790 597 787 268
Cuts at: 5845 1239
Size: 1524
Fragments arranged by size:
1790 1524 787 666 597 393 268 67
38
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2189 2189
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2104 2679 2691 2773 5277 1678
Size: 426 575 12 82 2504 2531
Fragments arranged by size:
2531 2504 575 426 82 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 2561 2564 2593
Size: 32 135 32 857 32 100 3 29
Cuts at: 2593 2596 4771 4803 4845 4877 1373
Size: 3 2175 32 42 32 2626
Fragments arranged by size:
2626 2175 857 135 100 42 32 32
32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2344 3102 748
Size: 1596 758 3776
Fragments arranged by size:
3776 1596 758
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KasI G'GCGC_C
Cuts at: 2675 2675
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 2648 2798 3197 3357 4088 4447
Size: 648 1267 215 150 399 160 731 359
Cuts at: 4447 4606 518
Size: 159 2042
Fragments arranged by size:
2042 1267 731 648 399 359 215 160
159 150
MspA1I CmG'CkG
Cuts at: 1606 2339 3546 4223 5371 5616 1606
Size: 733 1207 677 1148 245 2120
Fragments arranged by size:
2120 1207 1148 733 677 245
NarI GG'CG_CC
Cuts at: 2676 2676
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 2022 2985 513
Size: 552 957 963 3658
Fragments arranged by size:
3658 963 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2366 3202 3362 3488 5033 1132
Size: 97 1137 836 160 126 1545 2229
Fragments arranged by size:
2229 1545 1137 836 160 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3268 1108
Size: 2160 3970
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PmlI CAC'GTG
Cuts at: 2758 2758
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 2466 3700 2466
Size: 1234 4896
PstI C_TGCA'G
Cuts at: 830 3482 830
Size: 2652 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 2986 2986
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SexAI A'CCwGG_T
Cuts at: 2202 2823 2202
Size: 621 5509
SfcI C'TryA_G
Cuts at: 826 1045 2081 2403 3478 4613 5294 5485 826
Size: 219 1036 322 1075 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 1036 681 322 219 191
SfoI GGC'GCC
Cuts at: 2677 2677
Size: 6130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4234 5135 5397 820
Size: 197 926 2291 901 262 1553
Fragments arranged by size:
2291 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3427 3921 493
Size: 2934 494 2702
Fragments arranged by size:
2934 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3202 3488 1132
Size: 2070 286 3774
Fragments arranged by size:
3774 2070 286
SspI AAT'ATT
Cuts at: 5 52 3073 4054 5
Size: 47 3021 981 2081
Fragments arranged by size:
3021 2081 981 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2022 2140 2985 3569 513
Size: 552 957 118 845 584 3074
Fragments arranged by size:
3074 957 845 584 552 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3197 4157 4174 4327 4512 4957 325
Size: 1255 1617 960 17 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 960 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 3371 4376
Size: 274 80 33 51 1443 738 656 1005
Cuts at: 4376 6088 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1005 738 656 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 3161 4259 3161
Size: 1098 5032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2125 2297 3938 276
Size: 53 83 186 936 591 172 1641 2468
Fragments arranged by size:
2468 1641 936 591 186 172 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI