Restriction map for
pFN21ASDA0410
(Circular) (Six-base) MAPSORT of: pFN21ASDA0410.seq Check: 3714 from: 1 to: 6184
pFN21ASDA0410 6184 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 2768 2912 3043 3077 3215 4247 5631
Size: 697 439 144 131 34 138 1032 1384
Cuts at: 5631 5899 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1032 697 439 268 144 138
131 34
AflII C'TTAA_G
Cuts at: 820 1017 2864 820
Size: 197 1847 4140
Fragments arranged by size:
4140 1847 197
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 2069 1166
Size: 903 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3465 1184
Size: 2281 3903
AlwNI CAG_nnn'CTG
Cuts at: 2375 2902 3047 3927 5499 2375
Size: 527 145 880 1572 3060
Fragments arranged by size:
3060 1572 880 527 145
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 2801 3195 3493 3719 3871 5980 1493
Size: 1308 394 298 226 152 2109 1697
Fragments arranged by size:
2109 1697 1308 394 298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 3509 1870
Size: 73 1566 4545
Fragments arranged by size:
4545 1566 73
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3505 4960 618
Size: 325 217 627 1718 1455 1842
Fragments arranged by size:
1842 1718 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 2148 2223 3503 1391
Size: 672 85 75 1280 4072
Fragments arranged by size:
4072 1280 672 85 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2841 3017 928
Size: 971 942 176 4095
Fragments arranged by size:
4095 971 942 176
BbvCI CC'TCA_GC
Cuts at: 2410 2410
Size: 6184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 3601 3601
Size: 6184
Bme1580I G_kGCm'C
Cuts at: 1790 4245 5401 1790
Size: 2455 1156 2573
Fragments arranged by size:
2573 2455 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 3915 4873
Size: 416 53 83 399 24 836 1662 958
Cuts at: 4873 442
Size: 1753
Fragments arranged by size:
1753 1662 958 836 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2418 1056
Size: 1362 4822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2952 2952
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2163 2436 2780 4844 1239
Size: 924 273 344 2064 2579
Fragments arranged by size:
2579 2064 924 344 273
Bpu10I CC'TnA_GC
Cuts at: 2410 2545 2970 2410
Size: 135 425 5624
Fragments arranged by size:
5624 425 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3126 3153 4309 5174 5472 3126
Size: 27 1156 865 298 3838
Fragments arranged by size:
3838 1156 865 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3481 3975 493
Size: 711 2277 494 2702
Fragments arranged by size:
2702 2277 711 494
BsaBI GATnn'nnATC
Cuts at: 2757 6178 2757
Size: 3421 2763
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2179 2191 2209 2434
Size: 30 165 30 758 18 12 18 225
Cuts at: 2434 2464 3291 3321 1178
Size: 30 827 30 4041
Fragments arranged by size:
4041 827 758 225 165 30 30 30
30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2683 1186
Size: 1497 4687
BseYI C'CCAG_C
Cuts at: 5387 5883 5387
Size: 496 5688
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5611
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4245 4330 5401 4245
Size: 85 1071 5028
Fragments arranged by size:
5028 1071 85
BsmI GAATG_Cn'
Cuts at: 2614 2989 3467 3695 3788 2614
Size: 375 478 228 93 5010
Fragments arranged by size:
5010 478 375 228 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2148 2223 3503 4245 4330 5401
Size: 399 273 85 75 1280 742 85 1071
Cuts at: 5401 1391
Size: 2174
Fragments arranged by size:
2174 1280 1071 742 399 273 85 85
75
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 3241 4073 5803 3241
Size: 832 1730 3622
Fragments arranged by size:
3622 1730 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2245 2374 3539 844
Size: 1401 129 1165 3489
Fragments arranged by size:
3489 1401 1165 129
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2665 2865 4679 4853 65
Size: 1183 1417 200 1814 174 1396
Fragments arranged by size:
1814 1417 1396 1183 200 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 4828 5015 1700
Size: 110 268 2750 187 2869
Fragments arranged by size:
2869 2750 268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2375 3047 3413 794
Size: 1581 672 366 3565
Fragments arranged by size:
3565 1581 672 366
BstBI TT'CG_AA
Cuts at: 2007 4988 2007
Size: 2981 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2071 2071
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 2228 3514 3881 4266 4283 5735 5821 5833
Size: 256 1286 367 385 17 1452 86 12
Cuts at: 5833 6179 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1286 385 367 346 256 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2070 3417 513
Size: 552 1005 1347 3280
Fragments arranged by size:
3280 1347 1005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 3700 4494 4514 3700
Size: 794 20 5370
Fragments arranged by size:
5370 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2368 2403 529
Size: 35 1804 35 4310
Fragments arranged by size:
4310 1804 35 35
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2431 5191 809
Size: 507 1115 2760 1802
Fragments arranged by size:
2760 1802 1115 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 3631 4520
Size: 54 1425 50 275 18 243 1558 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1558 1425 889 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4114 1353
Size: 2761 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2329 2436 2768 2780 2912 3043
Size: 393 531 166 107 332 12 132 131
Cuts at: 3043 3077 3215 4247 4844 5631 5899 1239
Size: 34 138 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 787 597 531 393 332 268
166 138 132 131 107 34 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3453 3453
Size: 6184
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2879 2879
Size: 6184
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 5331 1678
Size: 3653 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2434 2466 4825 4857 4899
Size: 32 135 32 862 32 2359 32 42
Cuts at: 4899 4931 1373
Size: 32 2626
Fragments arranged by size:
2626 2359 862 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2622 3528 3774 669
Size: 988 281 684 906 246 3079
Fragments arranged by size:
3079 988 906 684 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5298 5482 1305
Size: 3993 184 2007
Fragments arranged by size:
3993 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4362
Fragments arranged by size:
4362 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 3036 3246 4142 4501 4660 518
Size: 648 903 967 210 896 359 159 2042
Fragments arranged by size:
2042 967 903 896 648 359 210 159
MspA1I CmG'CkG
Cuts at: 1606 3600 4277 5425 5670 1606
Size: 1994 677 1148 245 2120
Fragments arranged by size:
2120 1994 1148 677 245
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 6184
NcoI C'CATG_G
Cuts at: 513 1065 3417 513
Size: 552 2352 3280
Fragments arranged by size:
3280 2352 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 2414 1052
Size: 1362 4822
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3345 1108
Size: 2237 3947
PfoI T'CCnGG_A
Cuts at: 2048 2048
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PstI C_TGCA'G
Cuts at: 830 2332 2371 2656 3536 830
Size: 1502 39 285 880 3478
Fragments arranged by size:
3478 1502 880 285 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 2170 4432 6144 2170
Size: 2262 1712 2210
Fragments arranged by size:
2262 2210 1712
SfcI C'TryA_G
Cuts at: 826 1045 2292 2328 2367 2634 2652 3280 3453
Size: 219 1247 36 39 267 18 628 173
Cuts at: 3453 3532 4667 5348 5539 826
Size: 79 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 681 628 267 219 191
173 79 39 36 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 2078 1700
Size: 378 5806
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 3141 3168 4288 5189 5451
Size: 197 926 921 277 27 1120 901 262
Cuts at: 5451 820
Size: 1553
Fragments arranged by size:
1553 1120 926 921 901 277 262 197
27
SnaBI TAC'GTA
Cuts at: 493 3481 3975 493
Size: 2988 494 2702
Fragments arranged by size:
2988 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 2526 2667 1517
Size: 1009 141 5034
Fragments arranged by size:
5034 1009 141
StyI C'CwwG_G
Cuts at: 513 1065 2668 3274 3417 3623 513
Size: 552 1603 606 143 206 3074
Fragments arranged by size:
3074 1603 606 552 206 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 3373 4211 4228 4381 4566 5011
Size: 1255 897 896 838 17 153 185 445
Cuts at: 5011 325
Size: 1498
Fragments arranged by size:
1498 1255 897 896 838 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2168 2576 3099
Size: 274 80 33 51 1443 191 408 523
Cuts at: 3099 4430 6142 96
Size: 1331 1712 138
Fragments arranged by size:
1712 1443 1331 523 408 274 191 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6144 6176 6144
Size: 32 6152
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 2170 2848 3290 3467 4313 2170
Size: 678 442 177 846 4041
Fragments arranged by size:
4041 846 678 442 177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI