Restriction map for
pFN21ASDA0398
(Circular) (Six-base) MAPSORT of: pFN21ASDA0398.seq Check: 7498 from: 1 to: 6157
pFN21ASDA0398 6157 bp
With 182 enzymes: SgfI *
February 10, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3967 278
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2181 3192 3500 1937
Size: 244 1011 308 4594
Fragments arranged by size:
4594 1011 308 244
Acc65I G'GTAC_C
Cuts at: 3478 3478
Size: 6157
AclI AA'CG_TT
Cuts at: 1563 4284 4657 1563
Size: 2721 373 3063
Fragments arranged by size:
3063 2721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 2474 2997 4220 5604 5872 1632
Size: 705 137 523 1223 1384 268 1917
Fragments arranged by size:
1917 1384 1223 705 523 268 137
AflII C'TTAA_G
Cuts at: 820 1017 3388 820
Size: 197 2371 3589
Fragments arranged by size:
3589 2371 197
AflIII A'CryG_T
Cuts at: 1203 5056 6033 1203
Size: 3853 977 1327
Fragments arranged by size:
3853 1327 977
AgeI A'CCGG_T
Cuts at: 4988 4988
Size: 6157
AhdI GACnn_n'nnGTC
Cuts at: 4886 4886
Size: 6157
AleI CACnn'nnGTG
Cuts at: 1166 2848 1166
Size: 1682 4475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6157
AlwNI CAG_nnn'CTG
Cuts at: 2138 2471 3900 5472 2138
Size: 333 1429 1572 2823
Fragments arranged by size:
2823 1572 1429 333
ApaLI G'TGCA_C
Cuts at: 4214 5370 4214
Size: 1156 5001
ApoI r'AATT_y
Cuts at: 1493 2029 2089 2579 2789 2831 3053 3131 3212
Size: 536 60 490 210 42 222 78 81
Cuts at: 3212 3466 3692 3844 5953 1493
Size: 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 536 490 254 226 222 210
152 81 78 60 42
AseI AT'TA_AT
Cuts at: 160 1032 2118 4711 160
Size: 872 1086 2593 1606
Fragments arranged by size:
2593 1606 1086 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
AvaI C'yCGr_G
Cuts at: 1870 1943 3482 1870
Size: 73 1539 4545
Fragments arranged by size:
4545 1539 73
BamHI G'GATC_C
Cuts at: 3487 3854 3487
Size: 367 5790
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3478 4933 618
Size: 325 217 627 1691 1455 1842
Fragments arranged by size:
1842 1691 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3476 1391
Size: 2085 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 928
Size: 971 198 4988
Fragments arranged by size:
4988 971 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4348 4382 1903
Size: 34 2411 34 3678
Fragments arranged by size:
3678 2411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4049 5259 4049
Size: 1210 4947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2955 4768 136
Size: 107 122 71 631 605 1283 1813 1525
Fragments arranged by size:
1813 1525 1283 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6152 6152
Size: 6157
BlpI GC'TnA_GC
Cuts at: 3574 3574
Size: 6157
Bme1580I G_kGCm'C
Cuts at: 1790 4218 5374 1790
Size: 2428 1156 2573
Fragments arranged by size:
2573 2428 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6157
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2154 3888 4846
Size: 416 53 83 399 24 737 1734 958
Cuts at: 4846 442
Size: 1753
Fragments arranged by size:
1753 1734 958 737 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 4817 1239
Size: 1161 2417 2579
Fragments arranged by size:
2579 2417 1161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 4282 5147 5445 2435
Size: 1847 865 298 3147
Fragments arranged by size:
3147 1847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4820 882
Size: 617 3321 2219
Fragments arranged by size:
3321 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3454 3948 493
Size: 711 2250 494 2702
Fragments arranged by size:
2702 2250 711 494
BsaBI GATnn'nnATC
Cuts at: 6151 6151
Size: 6157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3001 3964 4346 4981
Size: 53 83 186 936 1468 963 382 635
Cuts at: 4981 275
Size: 1451
Fragments arranged by size:
1468 1451 963 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4590 4988 5262 5409 5820 4590
Size: 398 274 147 411 4927
Fragments arranged by size:
4927 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5932
Fragments arranged by size:
5932 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6157
BseYI C'CCAG_C
Cuts at: 5360 5856 5360
Size: 496 5661
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2805 1163
Size: 573 1069 4515
Fragments arranged by size:
4515 1069 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3607 4368 5396 1540
Size: 275 186 1606 761 1028 2301
Fragments arranged by size:
2301 1606 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4218 4303 5374 4218
Size: 85 1071 5001
Fragments arranged by size:
5001 1071 85
BsmI GAATG_Cn'
Cuts at: 3270 3668 3761 3270
Size: 398 93 5666
Fragments arranged by size:
5666 398 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3476 4218 4303 5374 1391
Size: 399 1686 742 85 1071 2174
Fragments arranged by size:
2174 1686 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5820 5820
Size: 6157
BspHI T'CATG_A
Cuts at: 4046 5776 4046
Size: 1730 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3512 844
Size: 2668 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4044 1394
Size: 429 2221 3507
Fragments arranged by size:
3507 2221 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3149 4652 4826 65
Size: 1183 1901 1503 174 1396
Fragments arranged by size:
1901 1503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4801 4988 1700
Size: 110 2991 187 2869
Fragments arranged by size:
2991 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6157
BssSI C'ACGA_G
Cuts at: 4217 5229 4217
Size: 1012 5145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2033 794
Size: 1239 4918
BstBI TT'CG_AA
Cuts at: 2007 4961 2007
Size: 2954 3203
BstEII G'GTnAC_C
Cuts at: 1184 3372 1184
Size: 2188 3969
BstYI r'GATC_y
Cuts at: 1972 3487 3854 4239 4256 5708 5794 5806 6152
Size: 1515 367 385 17 1452 86 12 346
Cuts at: 6152 1972
Size: 1977
Fragments arranged by size:
1977 1515 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4648
Fragments arranged by size:
4648 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5418
Fragments arranged by size:
5418 683 56
BtsI GCAGTG_nn'
Cuts at: 3673 4467 4487 3673
Size: 794 20 5343
Fragments arranged by size:
5343 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6157
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6122
DraI TTT'AAA
Cuts at: 3461 3461
Size: 6157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5164 809
Size: 507 3848 1802
Fragments arranged by size:
3848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3604 4493 6122
Size: 54 1425 50 275 18 1774 889 1629
Cuts at: 6122 8
Size: 43
Fragments arranged by size:
1774 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3604 1537
Size: 275 1792 4090
Fragments arranged by size:
4090 1792 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 4087 1353
Size: 905 1829 3423
Fragments arranged by size:
3423 1829 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4744 5118 5264 387
Size: 1064 138 3155 374 146 1280
Fragments arranged by size:
3155 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2400 2474 2997 4220 4817 5604
Size: 393 705 63 74 523 1223 597 787
Cuts at: 5604 5872 1239
Size: 268 1524
Fragments arranged by size:
1524 1223 787 705 597 523 393 268
74 63
EcoRI G'AATT_C
Cuts at: 2089 3131 3212 3466 3844 2089
Size: 1042 81 254 378 4402
Fragments arranged by size:
4402 1042 378 254 81
EcoRV GAT'ATC
Cuts at: 2617 2617
Size: 6157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2234 3393 2234
Size: 1159 4998
FspI TGC'GCA
Cuts at: 4663 4663
Size: 6157
HaeII r_GCGC'y
Cuts at: 1678 5304 1678
Size: 3626 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 4798 4830 4872
Size: 32 135 32 668 32 2526 32 42
Cuts at: 4872 4904 1373
Size: 32 2626
Fragments arranged by size:
2626 2526 668 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3501 3747 669
Size: 988 281 1563 246 3079
Fragments arranged by size:
3079 1563 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3009 748
Size: 2261 3896
HpaI GTT'AAC
Cuts at: 3747 3747
Size: 6157
KpnI G_GTAC'C
Cuts at: 3482 3482
Size: 6157
MfeI C'AATT_G
Cuts at: 3756 3756
Size: 6157
MluI A'CGCG_T
Cuts at: 6033 6033
Size: 6157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5271 5455 1305
Size: 3966 184 2007
Fragments arranged by size:
3966 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4335
Fragments arranged by size:
4335 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 2848 4115 4474 4633 518
Size: 648 1315 367 1267 359 159 2042
Fragments arranged by size:
2042 1315 1267 648 367 359 159
MspA1I CmG'CkG
Cuts at: 1606 2737 3573 4250 5398 5643 1606
Size: 1131 836 677 1148 245 2120
Fragments arranged by size:
2120 1148 1131 836 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4648
Fragments arranged by size:
4648 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6157
NotI GC'GGCC_GC
Cuts at: 3604 3604
Size: 6157
NspI r_CATG'y
Cuts at: 1132 1229 3341 3515 5060 1132
Size: 97 2112 174 1545 2229
Fragments arranged by size:
2229 2112 1545 174 97
PasI CC'CwG_GG
Cuts at: 1348 2951 1348
Size: 1603 4554
PciI A'CATG_T
Cuts at: 5056 5056
Size: 6157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6157
PmeI GTTT'AAAC
Cuts at: 3461 3461
Size: 6157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 5739 5771 4226
Size: 32 1481 32 4612
Fragments arranged by size:
4612 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2165 1613
Size: 552 5605
PsiI TTA'TAA
Cuts at: 2795 3727 2795
Size: 932 5225
PstI C_TGCA'G
Cuts at: 830 2867 3509 830
Size: 2037 642 3478
Fragments arranged by size:
3478 2037 642
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6157
SalI G'TCGA_C
Cuts at: 1936 3499 1936
Size: 1563 4594
SbfI CC_TGCA'GG
Cuts at: 3509 3509
Size: 6157
ScaI AGT'ACT
Cuts at: 4405 6117 4405
Size: 1712 4445
SfcI C'TryA_G
Cuts at: 826 1045 2170 2863 3505 4640 5321 5512 826
Size: 219 1125 693 642 1135 681 191 1471
Fragments arranged by size:
1471 1135 1125 693 681 642 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6157
SmaI CCC'GGG
Cuts at: 1872 3484 1872
Size: 1612 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 3388 4261 5162 5424 820
Size: 197 926 471 974 873 901 262 1553
Fragments arranged by size:
1553 974 926 901 873 471 262 197
SnaBI TAC'GTA
Cuts at: 493 3454 3948 493
Size: 2961 494 2702
Fragments arranged by size:
2961 2702 494
SpeI A'CTAG_T
Cuts at: 152 3187 152
Size: 3035 3122
SphI G_CATG'C
Cuts at: 1132 3515 1132
Size: 2383 3774
SspI AAT'ATT
Cuts at: 5 52 2116 4081 5
Size: 47 2064 1965 2081
Fragments arranged by size:
2081 2064 1965 47
StuI AGG'CCT
Cuts at: 1517 3272 1517
Size: 1755 4402
StyI C'CwwG_G
Cuts at: 513 1065 2022 3273 3596 513
Size: 552 957 1251 323 3074
Fragments arranged by size:
3074 1251 957 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4184 4201 4354 4539 4984 325
Size: 1255 2604 17 153 185 445 1498
Fragments arranged by size:
2604 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2696 3174 3348
Size: 274 80 33 51 1443 719 478 174
Cuts at: 3348 4403 6115 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 719 478 274 174 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2548 2580 6117 6149 2548
Size: 32 3537 32 2556
Fragments arranged by size:
3537 2556 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6157
XbaI T'CTAG_A
Cuts at: 3493 3493
Size: 6157
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6157
XmaI C'CCGG_G
Cuts at: 1870 3482 1870
Size: 1612 4545
XmnI GAAnn'nnTTC
Cuts at: 4286 4286
Size: 6157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3965 276
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI