Restriction map for
pFN21ASDA0390
(Circular) (Six-base) MAPSORT of: pFN21ASDA0390.seq Check: 6516 from: 1 to: 8629
pFN21ASDA0390 8629 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2559 2559
Size: 8629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6439 278
Size: 53 83 186 936 4903 2468
Fragments arranged by size:
4903 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5972 1937
Size: 4035 4594
Acc65I G'GTAC_C
Cuts at: 3309 5950 3309
Size: 2641 5988
AclI AA'CG_TT
Cuts at: 1563 6756 7129 1563
Size: 5193 373 3063
Fragments arranged by size:
5193 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2714 4555 5466 6692 8076 8344 1632
Size: 1082 1841 911 1226 1384 268 1917
Fragments arranged by size:
1917 1841 1384 1226 1082 911 268
AfeI AGC'GCT
Cuts at: 2435 3417 4239 2435
Size: 982 822 6825
Fragments arranged by size:
6825 982 822
AflII C'TTAA_G
Cuts at: 820 1017 4319 820
Size: 197 3302 5130
Fragments arranged by size:
5130 3302 197
AflIII A'CryG_T
Cuts at: 1203 3720 7528 8505 1203
Size: 2517 3808 977 1327
Fragments arranged by size:
3808 2517 1327 977
AgeI A'CCGG_T
Cuts at: 7460 7460
Size: 8629
AhdI GACnn_n'nnGTC
Cuts at: 4057 7358 4057
Size: 3301 5328
AleI CACnn'nnGTG
Cuts at: 1166 2085 3782 3972 4344 1166
Size: 919 1697 190 372 5451
Fragments arranged by size:
5451 1697 919 372 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2690 3564 1184
Size: 1506 874 6249
Fragments arranged by size:
6249 1506 874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 8597
AlwNI CAG_nnn'CTG
Cuts at: 2128 2353 5409 5683 5794 6372 7944 2128
Size: 225 3056 274 111 578 1572 2813
Fragments arranged by size:
3056 2813 1572 578 274 225 111
ApaI G_GGCC'C
Cuts at: 3023 5787 3023
Size: 2764 5865
ApaLI G'TGCA_C
Cuts at: 2926 3965 4325 6686 7842 2926
Size: 1039 360 2361 1156 3713
Fragments arranged by size:
3713 2361 1156 1039 360
ApoI r'AATT_y
Cuts at: 1493 4759 5884 5938 6164 6316 8425 1493
Size: 3266 1125 54 226 152 2109 1697
Fragments arranged by size:
3266 2109 1697 1125 226 152 54
AseI AT'TA_AT
Cuts at: 160 1032 7183 160
Size: 872 6151 1606
Fragments arranged by size:
6151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8629
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2189 3248 3869 4042 4630 4984
Size: 73 222 24 1059 621 173 588 354
Cuts at: 4984 5157 5954 1870
Size: 173 797 4545
Fragments arranged by size:
4545 1059 797 621 588 354 222 173
173 73 24
BamHI G'GATC_C
Cuts at: 5959 6326 5959
Size: 367 8262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 3253 3309 5660 5950
Size: 325 217 627 998 468 56 2351 290
Cuts at: 5950 7405 618
Size: 1455 1842
Fragments arranged by size:
2351 1842 1455 998 627 468 325 290
217 56
BanII G_rGCy'C
Cuts at: 1391 2079 2172 2967 3023 3177 3369 4094 4265
Size: 688 93 795 56 154 192 725 171
Cuts at: 4265 4991 5067 5787 5948 1391
Size: 726 76 720 161 4072
Fragments arranged by size:
4072 795 726 725 720 688 192 171
161 154 93 76 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 3675 4644 5726 928
Size: 971 147 1629 969 1082 3831
Fragments arranged by size:
3831 1629 1082 971 969 147
BbvCI CC'TCA_GC
Cuts at: 5612 5612
Size: 8629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5794 5828 6820 6854 1903
Size: 34 3857 34 992 34 3678
Fragments arranged by size:
3857 3678 992 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4329 6521 7731 4329
Size: 2192 1210 5227
Fragments arranged by size:
5227 2192 1210
BclI T'GATC_A
Cuts at: 1548 5226 1548
Size: 3678 4951
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2717 2726 2750
Size: 107 122 71 631 605 1045 9 24
Cuts at: 2750 2834 3005 3027 5162 7240 136
Size: 84 171 22 2135 2078 1525
Fragments arranged by size:
2135 2078 1525 1045 631 605 171 122
107 84 71 24 22 9
BglII A'GATC_T
Cuts at: 3150 4214 8624 3150
Size: 1064 4410 3155
Fragments arranged by size:
4410 3155 1064
BlpI GC'TnA_GC
Cuts at: 2073 2150 5848 6046 2073
Size: 77 3698 198 4656
Fragments arranged by size:
4656 3698 198 77
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2420 2504 2790 2930 3023 3264 3923
Size: 445 185 84 286 140 93 241 659
Cuts at: 3923 3969 4153 4329 5787 6690 7846 1790
Size: 46 184 176 1458 903 1156 2573
Fragments arranged by size:
2573 1458 1156 903 659 445 286 241
185 184 176 140 93 84 46
BmgBI CAC'GTC
Cuts at: 1318 2773 1318
Size: 1455 7174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4057 4893 6360
Size: 416 53 83 399 24 2640 836 1467
Cuts at: 6360 7318 442
Size: 958 1753
Fragments arranged by size:
2640 1753 1467 958 836 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8629
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4681 4148
Size: 533 8096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2648 3289 3427 4641 5066 5386 7289
Size: 995 414 641 138 1214 425 320 1903
Cuts at: 7289 1239
Size: 2579
Fragments arranged by size:
2579 1903 1214 995 641 425 414 320
138
Bpu10I CC'TnA_GC
Cuts at: 3359 5392 5612 3359
Size: 2033 220 6376
Fragments arranged by size:
6376 2033 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 3086 3170 5258 6754 7619 7917 2525
Size: 561 84 2088 1496 865 298 3237
Fragments arranged by size:
3237 2088 1496 865 561 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3792 4559 5692 7292 882
Size: 617 2293 767 1133 1600 2219
Fragments arranged by size:
2293 2219 1600 1133 767 617
BsaAI yAC'GTr
Cuts at: 493 1204 2230 2977 3721 4018 5320 5926 6420
Size: 711 1026 747 744 297 1302 606 494
Cuts at: 6420 493
Size: 2702
Fragments arranged by size:
2702 1302 1026 747 744 711 606 494
297
BsaBI GATnn'nnATC
Cuts at: 8623 8623
Size: 8629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2391 3459 4224 6436
Size: 53 83 186 936 858 1068 765 2212
Cuts at: 6436 6818 7453 275
Size: 382 635 1451
Fragments arranged by size:
2212 1451 1068 936 858 765 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4302 5581 5707 7062 7460 7734 7881 8292 4302
Size: 1279 126 1355 398 274 147 411 4639
Fragments arranged by size:
4639 1355 1279 411 398 274 147 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3113 3143 3164 3194 3287
Size: 30 165 30 1710 30 21 30 93
Cuts at: 3287 3317 3464 3494 3707 3737 4049 4079 5501
Size: 30 147 30 213 30 312 30 1422
Cuts at: 5501 5519 5531 5549 5702 5732 1178
Size: 18 12 18 153 30 4075
Fragments arranged by size:
4075 1710 1422 312 213 165 153 147
93 30 30 30 30 30 30 30
30 30 21 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2231 2618 2645 2897 2972 2975 4016
Size: 1021 24 387 27 252 75 3 1041
Cuts at: 4016 4084 4124 4165 4177 4217 4590 4606 4904
Size: 68 40 41 12 40 373 16 298
Cuts at: 4904 4981 5509 5527 1186
Size: 77 528 18 4288
Fragments arranged by size:
4288 1041 1021 528 387 373 298 252
77 75 68 41 40 40 27 24
18 16 12 3
BseYI C'CCAG_C
Cuts at: 2145 2284 2566 2712 3239 3368 3418 4208 4448
Size: 139 282 146 527 129 50 790 240
Cuts at: 4448 5667 7832 8328 2145
Size: 1219 2165 496 2446
Fragments arranged by size:
2446 2165 1219 790 527 496 282 240
146 139 129 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2447 2603 2719 2831 3998 5024
Size: 573 378 333 156 116 112 1167 1026
Cuts at: 5024 1163
Size: 4768
Fragments arranged by size:
4768 1167 1026 573 378 333 156 116
112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2687 3000 3160 3934 6079 6840
Size: 275 186 686 313 160 774 2145 761
Cuts at: 6840 7868 1540
Size: 1028 2301
Fragments arranged by size:
2301 2145 1028 774 761 686 313 275
186 160
BsiHKAI G_wGCw'C
Cuts at: 2172 2930 2967 3141 3969 4329 5067 6690 6775
Size: 758 37 174 828 360 738 1623 85
Cuts at: 6775 7846 2172
Size: 1071 2955
Fragments arranged by size:
2955 1623 1071 828 758 738 360 174
85 37
BsmI GAATG_Cn'
Cuts at: 6140 6233 6140
Size: 93 8536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2172 2235 2420 2504 2790 2930
Size: 399 289 93 63 185 84 286 140
Cuts at: 2930 2967 3023 3141 3177 3264 3369 3923 3969
Size: 37 56 118 36 87 105 554 46
Cuts at: 3969 4094 4153 4265 4329 4991 5067 5787 5948
Size: 125 59 112 64 662 76 720 161
Cuts at: 5948 6690 6775 7846 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 662 554 399 289
286 185 161 140 125 118 112 105
93 87 85 84 76 64 63 59
56 46 37 36
BspEI T'CCGG_A
Cuts at: 4302 8292 4302
Size: 3990 4639
BspHI T'CATG_A
Cuts at: 3336 6518 8248 3336
Size: 3182 1730 3717
Fragments arranged by size:
3717 3182 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 3810 3888 5984 844
Size: 1715 1251 78 2096 3489
Fragments arranged by size:
3489 2096 1715 1251 78
BsrBI CCG'CTC
Cuts at: 1394 1823 2241 3535 4033 4077 4116 4435 5301
Size: 429 418 1294 498 44 39 319 866
Cuts at: 5301 5779 6516 1394
Size: 478 737 3507
Fragments arranged by size:
3507 1294 866 737 498 478 429 418
319 44 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 5013 5511 7124 7298 65
Size: 1183 3765 498 1613 174 1396
Fragments arranged by size:
3765 1613 1396 1183 498 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3159 3353 4220 4382 5312 7273 7460
Size: 110 1349 194 867 162 930 1961 187
Cuts at: 7460 1700
Size: 2869
Fragments arranged by size:
2869 1961 1349 930 867 194 187 162
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8629
BssHII G'CGCG_C
Cuts at: 1925 2635 1925
Size: 710 7919
BssSI C'ACGA_G
Cuts at: 2621 3503 5479 6689 7701 2621
Size: 882 1976 1210 1012 3549
Fragments arranged by size:
3549 1976 1210 1012 882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2934 3652 3754 794
Size: 2140 718 102 5669
Fragments arranged by size:
5669 2140 718 102
BstBI TT'CG_AA
Cuts at: 2007 7433 2007
Size: 5426 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8629
BstXI CCAn_nnnn'nTGG
Cuts at: 2087 2284 3380 3613 5095 2087
Size: 197 1096 233 1482 5621
Fragments arranged by size:
5621 1482 1096 233 197
BstYI r'GATC_y
Cuts at: 1972 3150 4214 5959 6326 6711 6728 8180 8266
Size: 1178 1064 1745 367 385 17 1452 86
Cuts at: 8266 8278 8624 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1745 1452 1178 1064 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4331 4535 5825 4331
Size: 204 1290 7135
Fragments arranged by size:
7135 1290 204
BtgI C'CryG_G
Cuts at: 513 1065 2022 2250 3264 3454 3618 4641 4870
Size: 552 957 228 1014 190 164 1023 229
Cuts at: 4870 5058 513
Size: 188 4084
Fragments arranged by size:
4084 1023 1014 957 552 229 228 190
188 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5646 487
Size: 683 56 4420 3470
Fragments arranged by size:
4420 3470 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 4054 4601 5723 6145 6939 6959 3139
Size: 915 547 1122 422 794 20 4809
Fragments arranged by size:
4809 1122 915 794 547 422 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2569 2604 3013 3048 529
Size: 35 2005 35 409 35 6110
Fragments arranged by size:
6110 2005 409 35 35 35
DraI TTT'AAA
Cuts at: 5933 5933
Size: 8629
DraIII CAC_nnn'GTG
Cuts at: 2785 2934 3918 3958 4876 2785
Size: 149 984 40 918 6538
Fragments arranged by size:
6538 984 918 149 40
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4796 7636 809
Size: 507 3480 2840 1802
Fragments arranged by size:
3480 2840 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2242 2274
Size: 54 1425 50 275 18 274 138 32
Cuts at: 2274 2684 2727 2997 3045 3157 3603 3615 3931
Size: 410 43 270 48 112 446 12 316
Cuts at: 3931 4266 4964 5314 6076 6965 8594 8
Size: 335 698 350 762 889 1629 43
Fragments arranged by size:
1629 1425 889 762 698 446 410 350
335 316 275 274 270 138 112 54
50 48 43 43 32 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2684 2997 3157 3931 6076 1537
Size: 275 872 313 160 774 2145 4090
Fragments arranged by size:
4090 2145 872 774 313 275 160
EarI CTCTTCn'nnn_
Cuts at: 1353 4720 5291 5442 5556 6559 1353
Size: 3367 571 151 114 1003 3423
Fragments arranged by size:
3423 3367 1003 571 151 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 7216 7590 7736 387
Size: 1064 138 486 5141 374 146 1280
Fragments arranged by size:
5141 1280 1064 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 2170 2965 5065 2170
Size: 795 2100 5734
Fragments arranged by size:
5734 2100 795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2648 2714 3289 3427 4555 4641
Size: 393 602 414 66 575 138 1128 86
Cuts at: 4641 5066 5386 5466 6692 7289 8076 8344 1239
Size: 425 320 80 1226 597 787 268 1524
Fragments arranged by size:
1524 1226 1128 787 602 597 575 425
414 393 320 268 138 86 80 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2579 4915 2579
Size: 2336 6293
EcoO109I rG'GnC_Cy
Cuts at: 2719 3019 3020 3887 4542 4580 4660 4770 5397
Size: 300 1 867 655 38 80 110 627
Cuts at: 5397 5749 5783 5784 5829 2719
Size: 352 34 1 45 5519
Fragments arranged by size:
5519 867 655 627 352 300 110 80
45 38 34 1 1
EcoRI G'AATT_C
Cuts at: 5938 6316 5938
Size: 378 8251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4524 4524
Size: 8629
FspI TGC'GCA
Cuts at: 2471 3116 7135 2471
Size: 645 4019 3965
Fragments arranged by size:
4019 3965 645
FspAI rTGC'GCAy
Cuts at: 2471 2471
Size: 8629
HaeII r_GCGC'y
Cuts at: 1678 2270 2437 3166 3419 4241 7776 1678
Size: 592 167 729 253 822 3535 2531
Fragments arranged by size:
3535 2531 822 729 592 253 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3464 3496 3830 3862 4043
Size: 32 135 32 1892 32 334 32 181
Cuts at: 4043 4049 4075 4081 4297 4329 4481 4513 4961
Size: 6 26 6 216 32 152 32 448
Cuts at: 4961 4993 5218 5250 5489 5501 5519 5521 5533
Size: 32 225 32 239 12 18 2 12
Cuts at: 5533 5551 7270 7302 7344 7376 1373
Size: 18 1719 32 42 32 2626
Fragments arranged by size:
2626 1892 1719 448 334 239 225 216
181 152 135 42 32 32 32 32
32 32 32 32 32 32 26 18
18 12 12 6 6 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5140 5973 6219 669
Size: 988 281 3202 833 246 3079
Fragments arranged by size:
3202 3079 988 833 281 246
HindIII A'AGCT_T
Cuts at: 748 4506 4779 748
Size: 3758 273 4598
Fragments arranged by size:
4598 3758 273
HpaI GTT'AAC
Cuts at: 6219 6219
Size: 8629
KpnI G_GTAC'C
Cuts at: 3313 5954 3313
Size: 2641 5988
MfeI C'AATT_G
Cuts at: 6228 6228
Size: 8629
MluI A'CGCG_T
Cuts at: 8505 8505
Size: 8629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4102 7743 7927 1305
Size: 2797 3641 184 2007
Fragments arranged by size:
3641 2797 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 3605 3617 10
Size: 54 1425 343 444 1329 12 5022
Fragments arranged by size:
5022 1425 1329 444 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 3617 3782 3972 4344 4488 4646
Size: 648 919 1532 165 190 372 144 158
Cuts at: 4646 6587 6946 7105 518
Size: 1941 359 159 2042
Fragments arranged by size:
2042 1941 1532 919 648 372 359 190
165 159 158 144
MspA1I CmG'CkG
Cuts at: 1606 2284 2659 2725 2847 3060 3514 3525 3652
Size: 678 375 66 122 213 454 11 127
Cuts at: 3652 4456 6045 6722 7870 8115 1606
Size: 804 1589 677 1148 245 2120
Fragments arranged by size:
2120 1589 1148 804 678 677 454 375
245 213 127 122 66 11
NaeI GCC'GGC
Cuts at: 3161 3355 4222 4384 3161
Size: 194 867 162 7406
Fragments arranged by size:
7406 867 194 162
NcoI C'CATG_G
Cuts at: 513 1065 2022 2250 3264 3618 5058 513
Size: 552 957 228 1014 354 1440 4084
Fragments arranged by size:
4084 1440 1014 957 552 354 228
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8629
NgoMIV G'CCGG_C
Cuts at: 3159 3353 4220 4382 3159
Size: 194 867 162 7406
Fragments arranged by size:
7406 867 194 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8629
NotI GC'GGCC_GC
Cuts at: 6076 6076
Size: 8629
NruI TCG'CGA
Cuts at: 4248 4248
Size: 8629
NspI r_CATG'y
Cuts at: 1132 1229 2470 3115 3277 4651 5815 5987 7532
Size: 97 1241 645 162 1374 1164 172 1545
Cuts at: 7532 1132
Size: 2229
Fragments arranged by size:
2229 1545 1374 1241 1164 645 172 162
97
PasI CC'CwG_GG
Cuts at: 1348 3010 1348
Size: 1662 6967
PciI A'CATG_T
Cuts at: 7528 7528
Size: 8629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 2566 3958 4876 5683 5794 1108
Size: 1143 315 1392 918 807 111 3943
Fragments arranged by size:
3943 1392 1143 918 807 315 111
PfoI T'CCnGG_A
Cuts at: 3462 3462
Size: 8629
PmeI GTTT'AAAC
Cuts at: 5933 5933
Size: 8629
PmlI CAC'GTG
Cuts at: 2230 2977 4018 5320 2230
Size: 747 1041 1302 5539
Fragments arranged by size:
5539 1302 1041 747
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 6698 6730 8211 8243 3164
Size: 32 3502 32 1481 32 3550
Fragments arranged by size:
3550 3502 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3887 4660 4770 5749 3887
Size: 773 110 979 6767
Fragments arranged by size:
6767 979 773 110
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8629
PsiI TTA'TAA
Cuts at: 6199 6199
Size: 8629
PspOMI G'GGCC_C
Cuts at: 3019 5783 3019
Size: 2764 5865
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2393 2425 2393
Size: 32 8597
PstI C_TGCA'G
Cuts at: 830 2679 2751 3327 3543 3657 3885 5652 5981
Size: 1849 72 576 216 114 228 1767 329
Cuts at: 5981 830
Size: 3478
Fragments arranged by size:
3478 1849 1767 576 329 228 216 114
72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8629
PvuII CAG'CTG
Cuts at: 2284 2659 3525 2284
Size: 375 866 7388
Fragments arranged by size:
7388 866 375
SacI G_AGCT'C
Cuts at: 2172 2967 5067 2172
Size: 795 2100 5734
Fragments arranged by size:
5734 2100 795
SalI G'TCGA_C
Cuts at: 1936 5971 1936
Size: 4035 4594
SapI GCTCTTCn'nnn_
Cuts at: 4720 5291 4720
Size: 571 8058
SbfI CC_TGCA'GG
Cuts at: 2751 3885 5981 2751
Size: 1134 2096 5399
Fragments arranged by size:
5399 2096 1134
ScaI AGT'ACT
Cuts at: 6877 8589 6877
Size: 1712 6917
SexAI A'CCwGG_T
Cuts at: 3954 3954
Size: 8629
SfcI C'TryA_G
Cuts at: 826 1045 2675 2747 3323 3539 3653 3881 5648
Size: 219 1630 72 576 216 114 228 1767
Cuts at: 5648 5833 5977 7112 7793 7984 826
Size: 185 144 1135 681 191 1471
Fragments arranged by size:
1767 1630 1471 1135 681 576 228 219
216 191 185 144 114 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2726 3005 2726
Size: 279 8350
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8629
SmaI CCC'GGG
Cuts at: 1872 4986 5159 5956 1872
Size: 3114 173 797 4545
Fragments arranged by size:
4545 3114 797 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3101 3185 3869 4319 5273
Size: 197 926 597 561 84 684 450 954
Cuts at: 5273 6733 7634 7896 820
Size: 1460 901 262 1553
Fragments arranged by size:
1553 1460 954 926 901 684 597 561
450 262 197 84
SnaBI TAC'GTA
Cuts at: 493 5926 6420 493
Size: 5433 494 2702
Fragments arranged by size:
5433 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8629
SphI G_CATG'C
Cuts at: 1132 5987 1132
Size: 4855 3774
SspI AAT'ATT
Cuts at: 5 52 6553 5
Size: 47 6501 2081
Fragments arranged by size:
6501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8629
StyI C'CwwG_G
Cuts at: 513 1065 2022 2044 2250 3264 3552 3618 4765
Size: 552 957 22 206 1014 288 66 1147
Cuts at: 4765 5058 5263 5541 5754 6068 513
Size: 293 205 278 213 314 3074
Fragments arranged by size:
3074 1147 1014 957 552 314 293 288
278 213 206 205 66 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4721 5809 6656 6673 6826 7011 7456
Size: 1255 3141 1088 847 17 153 185 445
Cuts at: 7456 325
Size: 1498
Fragments arranged by size:
3141 1498 1255 1088 847 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3300 6875 8587
Size: 274 80 33 51 1443 1323 3575 1712
Cuts at: 8587 96
Size: 138
Fragments arranged by size:
3575 1712 1443 1323 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 4675 4707 5702 5734 8589 8621 3113
Size: 32 1530 32 995 32 2855 32 3121
Fragments arranged by size:
3121 2855 1530 995 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8629
XbaI T'CTAG_A
Cuts at: 5236 5965 5236
Size: 729 7900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3445 4877 5065 3445
Size: 1432 188 7009
Fragments arranged by size:
7009 1432 188
XhoI C'TCGA_G
Cuts at: 1943 3869 1943
Size: 1926 6703
XmaI C'CCGG_G
Cuts at: 1870 4984 5157 5954 1870
Size: 3114 173 797 4545
Fragments arranged by size:
4545 3114 797 173
XmnI GAAnn'nnTTC
Cuts at: 2492 4298 6758 2492
Size: 1806 2460 4363
Fragments arranged by size:
4363 2460 1806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6437 276
Size: 53 83 186 936 4903 2468
Fragments arranged by size:
4903 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI KasI NarI NsiI PacI PspXI RsrII
SacII SanDI SfoI SrfI SwaI