Restriction map for
pFN21ASDA0385
(Circular) (Six-base) MAPSORT of: pFN21ASDA0385.seq Check: 3139 from: 1 to: 8839
pFN21ASDA0385 8839 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6649 278
Size: 53 83 186 936 5113 2468
Fragments arranged by size:
5113 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2100 3720 5055 5099 5628 6182 1937
Size: 163 1620 1335 44 529 554 4594
Fragments arranged by size:
4594 1620 1335 554 529 163 44
Acc65I G'GTAC_C
Cuts at: 2392 6160 2392
Size: 3768 5071
AclI AA'CG_TT
Cuts at: 1563 6966 7339 1563
Size: 5403 373 3063
Fragments arranged by size:
5403 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 3799 4799 6902 8286 8554 1632
Size: 954 1213 1000 2103 1384 268 1917
Fragments arranged by size:
2103 1917 1384 1213 1000 954 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8642
AflIII A'CryG_T
Cuts at: 1203 3500 3502 3559 7738 8715 1203
Size: 2297 2 57 4179 977 1327
Fragments arranged by size:
4179 2297 1327 977 57 2
AgeI A'CCGG_T
Cuts at: 7670 7670
Size: 8839
AhdI GACnn_n'nnGTC
Cuts at: 3411 7568 3411
Size: 4157 4682
AleI CACnn'nnGTG
Cuts at: 1166 3503 4227 5726 5774 1166
Size: 2337 724 1499 48 4231
Fragments arranged by size:
4231 2337 1499 724 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3026 4169 1184
Size: 1842 1143 5854
Fragments arranged by size:
5854 1842 1143
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 8807
AlwNI CAG_nnn'CTG
Cuts at: 2411 3666 3744 4116 4847 5348 6582 8154 2411
Size: 1255 78 372 731 501 1234 1572 3096
Fragments arranged by size:
3096 1572 1255 1234 731 501 372 78
ApaI G_GGCC'C
Cuts at: 2161 4320 2161
Size: 2159 6680
ApaLI G'TGCA_C
Cuts at: 2955 6896 8052 2955
Size: 3941 1156 3742
Fragments arranged by size:
3941 3742 1156
ApoI r'AATT_y
Cuts at: 1493 2108 2419 3338 4423 5924 6148 6374 6526
Size: 615 311 919 1085 1501 224 226 152
Cuts at: 6526 8635 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1501 1085 919 615 311 226
224 152
AseI AT'TA_AT
Cuts at: 160 1032 5043 7393 160
Size: 872 4011 2350 1606
Fragments arranged by size:
4011 2350 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8839
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2182 3064 4319 4944 5169 5583
Size: 73 205 34 882 1255 625 225 414
Cuts at: 5583 5760 6164 1870
Size: 177 404 4545
Fragments arranged by size:
4545 1255 882 625 414 404 225 205
177 73 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3491 3524 3491
Size: 33 8806
BamHI G'GATC_C
Cuts at: 3226 6169 6536 3226
Size: 2943 367 5529
Fragments arranged by size:
5529 2943 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2392 2939 4179 4684 4708
Size: 325 217 627 605 547 1240 505 24
Cuts at: 4708 5765 6160 7615 618
Size: 1057 395 1455 1842
Fragments arranged by size:
1842 1455 1240 1057 627 605 547 505
395 325 217 24
BanII G_rGCy'C
Cuts at: 1391 2161 2191 2537 2582 2660 3429 3639 4320
Size: 770 30 346 45 78 769 210 681
Cuts at: 4320 5208 5355 6158 1391
Size: 888 147 803 4072
Fragments arranged by size:
4072 888 803 770 769 681 346 210
147 78 45 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3269 4256 4451 4926 6129 928
Size: 971 1370 987 195 475 1203 3638
Fragments arranged by size:
3638 1370 1203 987 971 475 195
BbvCI CC'TCA_GC
Cuts at: 3015 4848 5697 3015
Size: 1833 849 6157
Fragments arranged by size:
6157 1833 849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7030 7064 1903
Size: 34 5093 34 3678
Fragments arranged by size:
5093 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4297 6731 7941 4297
Size: 2434 1210 5195
Fragments arranged by size:
5195 2434 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8839
BfrBI ATG'CAT
Cuts at: 4660 4660
Size: 8839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2869 7450 136
Size: 107 122 71 631 605 1197 4581 1525
Fragments arranged by size:
4581 1525 1197 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3099 8834 3099
Size: 5735 3104
BlpI GC'TnA_GC
Cuts at: 5341 5349 6256 5341
Size: 8 907 7924
Fragments arranged by size:
7924 907 8
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2942 2959 4320 4638 4719 5768 6900
Size: 371 781 17 1361 318 81 1049 1132
Cuts at: 6900 8056 1790
Size: 1156 2573
Fragments arranged by size:
2573 1361 1156 1132 1049 781 371 318
81 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 2037 3211
Size: 416 53 83 399 24 608 12 1174
Cuts at: 3211 3910 4625 5043 5365 5630 5795 6113 6570
Size: 699 715 418 322 265 165 318 457
Cuts at: 6570 7528 442
Size: 958 1753
Fragments arranged by size:
1753 1174 958 715 699 608 457 418
416 399 322 318 265 165 83 53
24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 8839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 2253 2442 3162 3288 4232 7499 1239
Size: 867 147 189 720 126 944 3267 2579
Fragments arranged by size:
3267 2579 944 867 720 189 147 126
Bpu10I CC'TnA_GC
Cuts at: 3015 3423 3788 4848 5697 3015
Size: 408 365 1060 849 6157
Fragments arranged by size:
6157 1060 849 408 365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 6964 7829 8127 2887
Size: 4077 865 298 3599
Fragments arranged by size:
4077 3599 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 2498 4208 4757 7502 882
Size: 617 583 416 1710 549 2745 2219
Fragments arranged by size:
2745 2219 1710 617 583 549 416
BsaAI yAC'GTr
Cuts at: 493 1204 3503 6136 6630 493
Size: 711 2299 2633 494 2702
Fragments arranged by size:
2702 2633 2299 711 494
BsaBI GATnn'nnATC
Cuts at: 4700 8833 4700
Size: 4133 4706
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4551 6646 7028 7663
Size: 53 83 186 936 3018 2095 382 635
Cuts at: 7663 275
Size: 1451
Fragments arranged by size:
3018 2095 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5472 5955 7272 7670 7944 8091 8502 5472
Size: 483 1317 398 274 147 411 5809
Fragments arranged by size:
5809 1317 483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3631 3661 4559 4589 1178
Size: 30 165 30 2228 30 898 30 5428
Fragments arranged by size:
5428 2228 898 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2516 2564 2567 2874 3419 4787 4865
Size: 1327 3 48 3 307 545 1368 78
Cuts at: 4865 5549 5606 5609 5636 1186
Size: 684 57 3 27 4389
Fragments arranged by size:
4389 1368 1327 684 545 307 78 57
48 27 3 3 3
BseYI C'CCAG_C
Cuts at: 2260 2266 2411 3195 3737 4036 4492 5241 8042
Size: 6 145 784 542 299 456 749 2801
Cuts at: 8042 8538 2260
Size: 496 2561
Fragments arranged by size:
2801 2561 784 749 542 496 456 299
145 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2655 3148 3263 4923 5267 1163
Size: 573 919 493 115 1660 344 4735
Fragments arranged by size:
4735 1660 919 573 493 344 115
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6289 7050 8078 1540
Size: 275 186 4288 761 1028 2301
Fragments arranged by size:
4288 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2959 3429 3927 4748 5355 5601 6900 6985 8056
Size: 470 498 821 607 246 1299 85 1071
Cuts at: 8056 2959
Size: 3742
Fragments arranged by size:
3742 1299 1071 821 607 498 470 246
85
BsmI GAATG_Cn'
Cuts at: 2799 3780 6350 6443 2799
Size: 981 2570 93 5195
Fragments arranged by size:
5195 2570 981 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4558 4558
Size: 8839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2191 2537 2582 2660 2942 2959
Size: 399 371 30 346 45 78 282 17
Cuts at: 2959 3429 3639 3927 4320 4638 4719 4748 5208
Size: 470 210 288 393 318 81 29 460
Cuts at: 5208 5355 5601 5768 6158 6900 6985 8056 1391
Size: 147 246 167 390 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 470 460 399 393 390
371 346 318 288 282 246 210 167
147 85 81 78 45 30 29 17
BspEI T'CCGG_A
Cuts at: 5955 8502 5955
Size: 2547 6292
BspHI T'CATG_A
Cuts at: 6728 8458 6728
Size: 1730 7109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 3479 4085 4161 5988 6194 844
Size: 1361 1274 606 76 1827 206 3489
Fragments arranged by size:
3489 1827 1361 1274 606 206 76
BsrBI CCG'CTC
Cuts at: 1394 1823 6726 1394
Size: 429 4903 3507
Fragments arranged by size:
4903 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2959 3298 7334 7508 65
Size: 1183 1711 339 4036 174 1396
Fragments arranged by size:
4036 1711 1396 1183 339 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3309 7483 7670 1700
Size: 110 1499 4174 187 2869
Fragments arranged by size:
4174 2869 1499 187 110
BsrGI T'GTAC_A
Cuts at: 96 4011 96
Size: 3915 4924
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8839
BssSI C'ACGA_G
Cuts at: 3281 3437 6899 7911 3281
Size: 156 3462 1012 4209
Fragments arranged by size:
4209 3462 1012 156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2339 3255 3666 5458 794
Size: 1545 916 411 1792 4175
Fragments arranged by size:
4175 1792 1545 916 411
BstBI TT'CG_AA
Cuts at: 2007 7643 2007
Size: 5636 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8839
BstXI CCAn_nnnn'nTGG
Cuts at: 3266 3519 4229 4738 4804 4987 5776 3266
Size: 253 710 509 66 183 789 6329
Fragments arranged by size:
6329 789 710 509 253 183 66
BstYI r'GATC_y
Cuts at: 1972 2119 3099 3226 4236 4434 4788 6169 6536
Size: 147 980 127 1010 198 354 1381 367
Cuts at: 6536 6921 6938 8390 8476 8488 8834 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1381 1010 980 385 367 354
346 198 147 127 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2319 2385 2433 2775 3771 3828 5088 2319
Size: 66 48 342 996 57 1260 6070
Fragments arranged by size:
6070 1260 996 342 66 57 48
BtgI C'CryG_G
Cuts at: 513 1065 2022 2311 2829 4962 6036 513
Size: 552 957 289 518 2133 1074 3316
Fragments arranged by size:
3316 2133 1074 957 552 518 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5986 487
Size: 683 56 4760 3340
Fragments arranged by size:
4760 3340 683 56
BtsI GCAGTG_nn'
Cuts at: 2474 2940 2972 4135 4265 4461 6355 7149 7169
Size: 466 32 1163 130 196 1894 794 20
Cuts at: 7169 2474
Size: 4144
Fragments arranged by size:
4144 1894 1163 794 466 196 130 32
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8804
DraI TTT'AAA
Cuts at: 6143 6143
Size: 8839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2234 3716 7846 809
Size: 507 918 1482 4130 1802
Fragments arranged by size:
4130 1802 1482 918 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4959 4977 6286
Size: 54 1425 50 275 18 3129 18 1309
Cuts at: 6286 7175 8804 8
Size: 889 1629 43
Fragments arranged by size:
3129 1629 1425 1309 889 275 54 50
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 6286 1537
Size: 275 4474 4090
Fragments arranged by size:
4474 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 2729 3026 3437 3818 4590 5840 6769
Size: 1137 239 297 411 381 772 1250 929
Cuts at: 6769 1353
Size: 3423
Fragments arranged by size:
3423 1250 1137 929 772 411 381 297
239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5795 7426 7800 7946 387
Size: 1064 138 4206 1631 374 146 1280
Fragments arranged by size:
4206 1631 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3427 5353 3427
Size: 1926 6913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2253 2442 2586 3162 3288 3799
Size: 393 474 147 189 144 576 126 511
Cuts at: 3799 4232 4799 6902 7499 8286 8554 1239
Size: 433 567 2103 597 787 268 1524
Fragments arranged by size:
2103 1524 787 597 576 567 511 474
433 393 268 189 147 144 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2367 2403 3188 3281 2367
Size: 36 785 93 7925
Fragments arranged by size:
7925 785 93 36
EcoO109I rG'GnC_Cy
Cuts at: 2158 3274 4404 5084 5831 2158
Size: 1116 1130 680 747 5166
Fragments arranged by size:
5166 1130 1116 747 680
EcoRI G'AATT_C
Cuts at: 5924 6148 6526 5924
Size: 224 378 8237
Fragments arranged by size:
8237 378 224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3601 4057 3601
Size: 456 8383
FspI TGC'GCA
Cuts at: 7345 7345
Size: 8839
HaeII r_GCGC'y
Cuts at: 1678 2881 2929 3208 4198 7986 1678
Size: 1203 48 279 990 3788 2531
Fragments arranged by size:
3788 2531 1203 990 279 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2980 3012 3397 3429 3539
Size: 32 135 32 1408 32 385 32 110
Cuts at: 3539 3571 4354 4386 4528 4559 4560 4591 4850
Size: 32 783 32 142 31 1 31 259
Cuts at: 4850 4882 4938 4970 5081 5113 5119 5151 7480
Size: 32 56 32 111 32 6 32 2329
Cuts at: 7480 7512 7554 7586 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2329 1408 783 385 259 142 135
111 110 56 42 32 32 32 32
32 32 32 32 32 32 32 32
31 31 6 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3709 6183 6429 669
Size: 988 281 1771 2474 246 3079
Fragments arranged by size:
3079 2474 1771 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3982 748
Size: 3234 5605
HpaI GTT'AAC
Cuts at: 6429 6429
Size: 8839
KpnI G_GTAC'C
Cuts at: 2396 6164 2396
Size: 3768 5071
MfeI C'AATT_G
Cuts at: 6438 6438
Size: 8839
MluI A'CGCG_T
Cuts at: 8715 8715
Size: 8839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 6033 7953 8137 1305
Size: 3516 1212 1920 184 2007
Fragments arranged by size:
3516 2007 1920 1212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4961 4979 10
Size: 54 1425 343 3129 18 3870
Fragments arranged by size:
3870 3129 1425 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 2945 3503 4227 4631 4643 5657
Size: 648 1638 141 558 724 404 12 1014
Cuts at: 5657 5726 5774 6797 7156 7315 518
Size: 69 48 1023 359 159 2042
Fragments arranged by size:
2042 1638 1023 1014 724 648 558 404
359 159 141 69 48 12
MspA1I CmG'CkG
Cuts at: 1606 3741 3969 5345 5696 5904 6255 6932 8080
Size: 2135 228 1376 351 208 351 677 1148
Cuts at: 8080 8325 1606
Size: 245 2120
Fragments arranged by size:
2135 2120 1376 1148 677 351 351 245
228 208
NcoI C'CATG_G
Cuts at: 513 1065 2022 2829 4962 6036 513
Size: 552 957 807 2133 1074 3316
Fragments arranged by size:
3316 2133 1074 957 807 552
NdeI CA'TA_TG
Cuts at: 387 3254 5218 387
Size: 2867 1964 4008
Fragments arranged by size:
4008 2867 1964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8839
NotI GC'GGCC_GC
Cuts at: 6286 6286
Size: 8839
NsiI A_TGCA'T
Cuts at: 4662 4662
Size: 8839
NspI r_CATG'y
Cuts at: 1132 1229 2893 3563 5710 6052 6064 6197 7742
Size: 97 1664 670 2147 342 12 133 1545
Cuts at: 7742 1132
Size: 2229
Fragments arranged by size:
2229 2147 1664 1545 670 342 133 97
12
PasI CC'CwG_GG
Cuts at: 1348 2540 1348
Size: 1192 7647
PciI A'CATG_T
Cuts at: 3559 7738 3559
Size: 4179 4660
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2411 2693 3744 4224 1108
Size: 1303 282 1051 480 5723
Fragments arranged by size:
5723 1303 1051 480 282
PfoI T'CCnGG_A
Cuts at: 2238 4914 5833 2238
Size: 2676 919 5244
Fragments arranged by size:
5244 2676 919
PmeI GTTT'AAAC
Cuts at: 6143 6143
Size: 8839
PmlI CAC'GTG
Cuts at: 3503 3503
Size: 8839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6908 6940 8421 8453 6908
Size: 32 1481 32 7294
Fragments arranged by size:
7294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3274 4404 5831 3274
Size: 1130 1427 6282
Fragments arranged by size:
6282 1427 1130
PshAI GACnn'nnGTC
Cuts at: 1613 2313 5829 1613
Size: 700 3516 4623
Fragments arranged by size:
4623 3516 700
PsiI TTA'TAA
Cuts at: 6409 6409
Size: 8839
PspOMI G'GGCC_C
Cuts at: 2157 4316 2157
Size: 2159 6680
PspXI vC'TCGA_Gb
Cuts at: 2148 2148
Size: 8839
PstI C_TGCA'G
Cuts at: 830 2481 2935 2979 3251 3320 3662 3734 3992
Size: 1651 454 44 272 69 342 72 258
Cuts at: 3992 4130 5691 6191 830
Size: 138 1561 500 3478
Fragments arranged by size:
3478 1651 1561 500 454 342 272 258
138 72 69 44
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8839
PvuII CAG'CTG
Cuts at: 3741 5345 5696 3741
Size: 1604 351 6884
Fragments arranged by size:
6884 1604 351
SacI G_AGCT'C
Cuts at: 3429 5355 3429
Size: 1926 6913
SalI G'TCGA_C
Cuts at: 1936 6181 1936
Size: 4245 4594
SapI GCTCTTCn'nnn_
Cuts at: 3026 3026
Size: 8839
SbfI CC_TGCA'GG
Cuts at: 6191 6191
Size: 8839
ScaI AGT'ACT
Cuts at: 5456 7087 8799 5456
Size: 1631 1712 5496
Fragments arranged by size:
5496 1712 1631
SfcI C'TryA_G
Cuts at: 826 1045 2477 2931 2975 3247 3316 3658 3730
Size: 219 1432 454 44 272 69 342 72
Cuts at: 3730 3988 4126 4276 5687 6187 7322 8003 8194
Size: 258 138 150 1411 500 1135 681 191
Cuts at: 8194 826
Size: 1471
Fragments arranged by size:
1471 1432 1411 1135 681 500 454 342
272 258 219 191 150 138 72 69
44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8839
SmaI CCC'GGG
Cuts at: 1872 5171 6166 1872
Size: 3299 995 4545
Fragments arranged by size:
4545 3299 995
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2148 2902 4944 5583 6943 7844
Size: 197 926 205 754 2042 639 1360 901
Cuts at: 7844 8106 820
Size: 262 1553
Fragments arranged by size:
2042 1553 1360 926 901 754 639 262
205 197
SnaBI TAC'GTA
Cuts at: 493 6136 6630 493
Size: 5643 494 2702
Fragments arranged by size:
5643 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8839
SphI G_CATG'C
Cuts at: 1132 6064 6197 1132
Size: 4932 133 3774
Fragments arranged by size:
4932 3774 133
SspI AAT'ATT
Cuts at: 5 52 6763 5
Size: 47 6711 2081
Fragments arranged by size:
6711 2081 47
StuI AGG'CCT
Cuts at: 1517 4865 1517
Size: 3348 5491
StyI C'CwwG_G
Cuts at: 513 1065 2022 2563 2829 3111 4737 4803 4962
Size: 552 957 541 266 282 1626 66 159
Cuts at: 4962 5772 6036 6278 513
Size: 810 264 242 3074
Fragments arranged by size:
3074 1626 957 810 552 541 282 266
264 242 159 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5233 6866 6883 7036 7221 7666 325
Size: 1255 3653 1633 17 153 185 445 1498
Fragments arranged by size:
3653 1633 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4011 4058 4665
Size: 274 80 33 51 1443 2034 47 607
Cuts at: 4665 5454 7085 8797 96
Size: 789 1631 1712 138
Fragments arranged by size:
2034 1712 1631 1443 789 607 274 138
80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3631 3663 8799 8831 3631
Size: 32 5136 32 3639
Fragments arranged by size:
5136 3639 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8839
XbaI T'CTAG_A
Cuts at: 6175 6175
Size: 8839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2151 2257 2151
Size: 106 8733
XhoI C'TCGA_G
Cuts at: 1943 2148 4944 5583 1943
Size: 205 2796 639 5199
Fragments arranged by size:
5199 2796 639 205
XmaI C'CCGG_G
Cuts at: 1870 5169 6164 1870
Size: 3299 995 4545
Fragments arranged by size:
4545 3299 995
XmnI GAAnn'nnTTC
Cuts at: 3983 5486 6968 3983
Size: 1503 1482 5854
Fragments arranged by size:
5854 1503 1482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6647 276
Size: 53 83 186 936 5113 2468
Fragments arranged by size:
5113 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BsiWI BstZ17I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI