Restriction map for
pFN21ASDA0380
(Circular) (Six-base) MAPSORT of: pFN21ASDA0380.seq Check: 7014 from: 1 to: 9295
pFN21ASDA0380 9295 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4278 4278
Size: 9295
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5213 7105 278
Size: 53 83 186 936 3677 1892 2468
Fragments arranged by size:
3677 2468 1892 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6638 1937
Size: 4701 4594
Acc65I G'GTAC_C
Cuts at: 6616 6616
Size: 9295
AclI AA'CG_TT
Cuts at: 1563 7422 7795 1563
Size: 5859 373 3063
Fragments arranged by size:
5859 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2993 4278 5057 5071 5967 6087 7358 8742
Size: 1361 1285 779 14 896 120 1271 1384
Cuts at: 8742 9010 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1361 1285 1271 896 779 268
120 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9098
AflIII A'CryG_T
Cuts at: 1203 5376 8194 9171 1203
Size: 4173 2818 977 1327
Fragments arranged by size:
4173 2818 1327 977
AgeI A'CCGG_T
Cuts at: 2855 8126 2855
Size: 5271 4024
AhdI GACnn_n'nnGTC
Cuts at: 2145 8024 2145
Size: 5879 3416
AleI CACnn'nnGTG
Cuts at: 1166 2309 2459 6410 1166
Size: 1143 150 3951 4051
Fragments arranged by size:
4051 3951 1143 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3402 3774 4524 1184
Size: 2218 372 750 5955
Fragments arranged by size:
5955 2218 750 372
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 9263
AlwNI CAG_nnn'CTG
Cuts at: 2762 2962 3770 5458 6056 7038 8610 2762
Size: 200 808 1688 598 982 1572 3447
Fragments arranged by size:
3447 1688 1572 982 808 598 200
ApaI G_GGCC'C
Cuts at: 4750 5180 5342 6519 4750
Size: 430 162 1177 7526
Fragments arranged by size:
7526 1177 430 162
ApaLI G'TGCA_C
Cuts at: 7352 8508 7352
Size: 1156 8139
ApoI r'AATT_y
Cuts at: 1493 5902 6604 6830 6982 9091 1493
Size: 4409 702 226 152 2109 1697
Fragments arranged by size:
4409 2109 1697 702 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7849 160
Size: 872 6817 1606
Fragments arranged by size:
6817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9295
AvaI C'yCGr_G
Cuts at: 1870 1943 2920 4335 4485 4749 5319 5711 6620
Size: 73 977 1415 150 264 570 392 909
Cuts at: 6620 1870
Size: 4545
Fragments arranged by size:
4545 1415 977 909 570 392 264 150
73
BamHI G'GATC_C
Cuts at: 2533 3474 6625 6992 2533
Size: 941 3151 367 4836
Fragments arranged by size:
4836 3151 941 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2357 4718 5271 6157
Size: 325 217 627 516 54 2361 553 886
Cuts at: 6157 6401 6616 8071 618
Size: 244 215 1455 1842
Fragments arranged by size:
2361 1842 1455 886 627 553 516 325
244 217 215 54
BanII G_rGCy'C
Cuts at: 1391 2387 2418 2446 2834 4750 5066 5180 5241
Size: 996 31 28 388 1916 316 114 61
Cuts at: 5241 5342 5613 5712 6170 6480 6519 6614 1391
Size: 101 271 99 458 310 39 95 4072
Fragments arranged by size:
4072 1916 996 458 388 316 310 271
114 101 99 95 61 39 31 28
BbeI G_GCGC'C
Cuts at: 2361 2361
Size: 9295
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2916 3426 3473 5862 6558 928
Size: 971 162 855 510 47 2389 696 3665
Fragments arranged by size:
3665 2389 971 855 696 510 162 47
BbvCI CC'TCA_GC
Cuts at: 2579 6000 6174 6210 6216 2579
Size: 3421 174 36 6 5658
Fragments arranged by size:
5658 3421 174 36 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7486 7520 1903
Size: 34 5549 34 3678
Fragments arranged by size:
5549 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7187 8397 7187
Size: 1210 8085
BclI T'GATC_A
Cuts at: 1548 2346 2793 1548
Size: 798 447 8050
Fragments arranged by size:
8050 798 447
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 2807 3870
Size: 107 122 71 631 605 589 546 1063
Cuts at: 3870 7906 136
Size: 4036 1525
Fragments arranged by size:
4036 1525 1063 631 605 589 546 122
107 71
BglII A'GATC_T
Cuts at: 5187 9290 5187
Size: 4103 5192
BlpI GC'TnA_GC
Cuts at: 6712 6712
Size: 9295
Bme1580I G_kGCm'C
Cuts at: 1790 3915 4750 5180 5342 6519 7356 8512 1790
Size: 2125 835 430 162 1177 837 1156 2573
Fragments arranged by size:
2573 2125 1177 1156 837 835 430 162
BmgBI CAC'GTC
Cuts at: 1318 2457 5377 6286 1318
Size: 1139 2920 909 4327
Fragments arranged by size:
4327 2920 1139 909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3027 3305 3514
Size: 416 53 83 399 24 1610 278 209
Cuts at: 3514 4065 5430 5513 6521 7026 7984 442
Size: 551 1365 83 1008 505 958 1753
Fragments arranged by size:
1753 1610 1365 1008 958 551 505 416
399 278 209 83 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4629 6189 6386 6550 4511
Size: 118 1560 197 164 7256
Fragments arranged by size:
7256 1560 197 164 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2265 2402 2984 3149 3836 3976 4163 4481
Size: 1026 137 582 165 687 140 187 318
Cuts at: 4481 4709 4877 4963 5009 5433 5852 5907 6478
Size: 228 168 86 46 424 419 55 571
Cuts at: 6478 7955 1239
Size: 1477 2579
Fragments arranged by size:
2579 1477 1026 687 582 571 424 419
318 228 187 168 165 140 137 86
55 46
Bpu10I CC'TnA_GC
Cuts at: 2579 5472 6000 6174 6210 6216 6471 2579
Size: 2893 528 174 36 6 255 5403
Fragments arranged by size:
5403 2893 528 255 174 36 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3219 3851 4034 4757 5084 6121 6308 6323 6356
Size: 632 183 723 327 1037 187 15 33
Cuts at: 6356 7420 8285 8583 3219
Size: 1064 865 298 3931
Fragments arranged by size:
3931 1064 1037 865 723 632 327 298
187 183 33 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2153 2632 3897 4014 4632 5870 7958
Size: 617 654 479 1265 117 618 1238 2088
Cuts at: 7958 882
Size: 2219
Fragments arranged by size:
2219 2088 1265 1238 654 618 617 479
117
BsaAI yAC'GTr
Cuts at: 493 1204 4978 6592 7086 493
Size: 711 3774 1614 494 2702
Fragments arranged by size:
3774 2702 1614 711 494
BsaBI GATnn'nnATC
Cuts at: 9289 9289
Size: 9295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 5210 6247 6540
Size: 53 83 186 936 825 2852 1037 293
Cuts at: 6540 7102 7484 8119 275
Size: 562 382 635 1451
Fragments arranged by size:
2852 1451 1037 936 825 635 562 382
293 186 83 53
BsaWI w'CCGG_w
Cuts at: 2283 2855 3791 7728 8126 8400 8547 8958 2283
Size: 572 936 3937 398 274 147 411 2620
Fragments arranged by size:
3937 2620 936 572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2361 2391 5759 5789 1178
Size: 30 165 30 958 30 3368 30 4684
Fragments arranged by size:
4684 3368 958 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 4355 5056 5417 6252 1186
Size: 1286 1883 701 361 835 4229
Fragments arranged by size:
4229 1883 1286 835 701 361
BseYI C'CCAG_C
Cuts at: 2434 2811 3301 3470 4199 4209 5173 5331 5348
Size: 377 490 169 729 10 964 158 17
Cuts at: 5348 5427 5817 6251 8498 8994 2434
Size: 79 390 434 2247 496 2735
Fragments arranged by size:
2735 2247 964 729 496 490 434 390
377 169 158 79 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2070 2249 2271 3078 3950 4069 4494
Size: 573 334 179 22 807 872 119 425
Cuts at: 4494 5146 5508 6012 1163
Size: 652 362 504 4446
Fragments arranged by size:
4446 872 807 652 573 504 425 362
334 179 119 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2284 3792 6745 7506 8534 1540
Size: 275 186 283 1508 2953 761 1028 2301
Fragments arranged by size:
2953 2301 1508 1028 761 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 2446 5100 5118 5241 7356 7441 8512 2446
Size: 2654 18 123 2115 85 1071 3229
Fragments arranged by size:
3229 2654 2115 1071 123 85 18
BsmI GAATG_Cn'
Cuts at: 5266 6806 6899 5266
Size: 1540 93 7662
Fragments arranged by size:
7662 1540 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2387 2418 2446 2834 3915 4750 5066
Size: 399 597 31 28 388 1081 835 316
Cuts at: 5066 5100 5118 5180 5241 5342 5613 5712 6170
Size: 34 18 62 61 101 271 99 458
Cuts at: 6170 6480 6519 6614 7356 7441 8512 1391
Size: 310 39 95 742 85 1071 2174
Fragments arranged by size:
2174 1081 1071 835 742 597 458 399
388 316 310 271 101 99 95 85
62 61 39 34 31 28 18
BspEI T'CCGG_A
Cuts at: 8958 8958
Size: 9295
BspHI T'CATG_A
Cuts at: 7184 8914 7184
Size: 1730 7565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3336 3819 4278 4374 5652 6650 844
Size: 2492 483 459 96 1278 998 3489
Fragments arranged by size:
3489 2492 1278 998 483 459 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 3918 5124 5624 6258 7182 1394
Size: 429 2032 63 1206 500 634 924 3507
Fragments arranged by size:
3507 2032 1206 924 634 500 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 3718 7790 7964 65
Size: 1183 2470 4072 174 1396
Fragments arranged by size:
4072 2470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2264 2652 2855 4224 7939 8126 1700
Size: 110 454 388 203 1369 3715 187 2869
Fragments arranged by size:
3715 2869 1369 454 388 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9295
BssSI C'ACGA_G
Cuts at: 7355 8367 7355
Size: 1012 8283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2236 794
Size: 1442 7853
BstBI TT'CG_AA
Cuts at: 2007 8099 2007
Size: 6092 3203
BstEII G'GTnAC_C
Cuts at: 1184 2034 2311 2858 1184
Size: 850 277 547 7621
Fragments arranged by size:
7621 850 547 277
BstXI CCAn_nnnn'nTGG
Cuts at: 4855 5533 5578 6412 4855
Size: 678 45 834 7738
Fragments arranged by size:
7738 834 678 45
BstYI r'GATC_y
Cuts at: 1972 2533 2562 2959 3156 3255 3267 3474 4900
Size: 561 29 397 197 99 12 207 1426
Cuts at: 4900 5187 6625 6992 7377 7394 8846 8932 8944
Size: 287 1438 367 385 17 1452 86 12
Cuts at: 8944 9290 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1438 1426 561 397 385 367
346 287 207 197 99 86 29 17
12 12
Bsu36I CC'TnA_GG
Cuts at: 2567 3804 5400 6278 6525 2567
Size: 1237 1596 878 247 5337
Fragments arranged by size:
5337 1596 1237 878 247
BtgI C'CryG_G
Cuts at: 513 1065 2307 2400 3214 3435 5383 5685 513
Size: 552 1242 93 814 221 1948 302 4123
Fragments arranged by size:
4123 1948 1242 814 552 302 221 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2099 487
Size: 683 56 873 7683
Fragments arranged by size:
7683 873 683 56
BtsI GCAGTG_nn'
Cuts at: 3038 3931 5491 6811 7605 7625 3038
Size: 893 1560 1320 794 20 4708
Fragments arranged by size:
4708 1560 1320 893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9260
DraI TTT'AAA
Cuts at: 6599 6599
Size: 9295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8302 809
Size: 507 6986 1802
Fragments arranged by size:
6986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2799 2981 5130
Size: 54 1425 50 275 18 969 182 2149
Cuts at: 5130 5843 6043 6742 7631 9260 8
Size: 713 200 699 889 1629 43
Fragments arranged by size:
2149 1629 1425 969 889 713 699 275
200 182 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6742 1537
Size: 275 4930 4090
Fragments arranged by size:
4930 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2392 3566 3664 3873 4361 4592 5241 5490
Size: 1039 1174 98 209 488 231 649 249
Cuts at: 5490 7225 1353
Size: 1735 3423
Fragments arranged by size:
3423 1735 1174 1039 649 488 249 231
209 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3041 6093 7882 8256 8402 387
Size: 1064 138 1452 3052 1789 374 146 1280
Fragments arranged by size:
3052 1789 1452 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2444 5239 2444
Size: 2795 6500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2402 2984 2993 3149 3836 3976
Size: 393 633 137 582 9 156 687 140
Cuts at: 3976 4163 4278 4481 4709 4877 4963 5009 5057
Size: 187 115 203 228 168 86 46 48
Cuts at: 5057 5071 5433 5852 5907 5967 6087 6478 7358
Size: 14 362 419 55 60 120 391 880
Cuts at: 7358 7955 8742 9010 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 880 787 687 633 597 582 419
393 391 362 268 228 203 187 168
156 140 137 120 115 86 60 55
48 46 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4112 5462 4112
Size: 1350 7945
EcoO109I rG'GnC_Cy
Cuts at: 2425 2516 3346 3808 4285 4430 4931 4993 5315
Size: 91 830 462 477 145 501 62 322
Cuts at: 5315 5338 5339 5424 5455 5467 5591 5767 6125
Size: 23 1 85 31 12 124 176 358
Cuts at: 6125 6515 6516 2425
Size: 390 1 5204
Fragments arranged by size:
5204 830 501 477 462 390 358 322
176 145 124 91 85 62 31 23
12 1 1
EcoRI G'AATT_C
Cuts at: 6604 6982 6604
Size: 378 8917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3619 5101 3619
Size: 1482 7813
FspI TGC'GCA
Cuts at: 4274 7801 4274
Size: 3527 5768
HaeII r_GCGC'y
Cuts at: 1678 2361 3364 3432 8442 1678
Size: 683 1003 68 5010 2531
Fragments arranged by size:
5010 2531 1003 683 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2131 2163 3141
Size: 32 135 32 512 32 15 32 978
Cuts at: 3141 3173 3389 3421 4569 4601 4629 4661 4885
Size: 32 216 32 1148 32 28 32 224
Cuts at: 4885 4917 5640 5672 5759 5791 6193 6225 7936
Size: 32 723 32 87 32 402 32 1711
Cuts at: 7936 7968 8010 8042 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1711 1148 978 723 512 402 224
216 135 87 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 28 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2299 3934 6639 6885 669
Size: 988 281 361 1635 2705 246 3079
Fragments arranged by size:
3079 2705 1635 988 361 281 246
HindIII A'AGCT_T
Cuts at: 748 3097 3796 4260 748
Size: 2349 699 464 5783
Fragments arranged by size:
5783 2349 699 464
HpaI GTT'AAC
Cuts at: 6885 6885
Size: 9295
KasI G'GCGC_C
Cuts at: 2357 2357
Size: 9295
KpnI G_GTAC'C
Cuts at: 6620 6620
Size: 9295
MfeI C'AATT_G
Cuts at: 6894 6894
Size: 9295
MluI A'CGCG_T
Cuts at: 9171 9171
Size: 9295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3525 8409 8593 1305
Size: 2220 4884 184 2007
Fragments arranged by size:
4884 2220 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2801 5132 6045 10
Size: 54 1425 343 969 2331 913 3260
Fragments arranged by size:
3260 2331 1425 969 913 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 2459 5231 6410 7253 7612 7771
Size: 648 1143 150 2772 1179 843 359 159
Cuts at: 7771 518
Size: 2042
Fragments arranged by size:
2772 2042 1179 1143 843 648 359 159
150
MspA1I CmG'CkG
Cuts at: 1606 3382 3509 4132 4213 4633 4663 4681 6220
Size: 1776 127 623 81 420 30 18 1539
Cuts at: 6220 6711 7388 8536 8781 1606
Size: 491 677 1148 245 2120
Fragments arranged by size:
2120 1776 1539 1148 677 623 491 420
245 127 81 30 18
NaeI GCC'GGC
Cuts at: 2654 2654
Size: 9295
NarI GG'CG_CC
Cuts at: 2358 2358
Size: 9295
NcoI C'CATG_G
Cuts at: 513 1065 2307 2400 3435 5383 5685 513
Size: 552 1242 93 1035 1948 302 4123
Fragments arranged by size:
4123 1948 1242 1035 552 302 93
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9295
NgoMIV G'CCGG_C
Cuts at: 2652 2652
Size: 9295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9295
NotI GC'GGCC_GC
Cuts at: 6742 6742
Size: 9295
NspI r_CATG'y
Cuts at: 1132 1229 2555 6118 6653 8198 1132
Size: 97 1326 3563 535 1545 2229
Fragments arranged by size:
3563 2229 1545 1326 535 97
PasI CC'CwG_GG
Cuts at: 1348 2762 5763 5780 5795 1348
Size: 1414 3001 17 15 4848
Fragments arranged by size:
4848 3001 1414 17 15
PciI A'CATG_T
Cuts at: 8194 8194
Size: 9295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2621 2962 3770 4504 6041 6511 1108
Size: 1513 341 808 734 1537 470 3892
Fragments arranged by size:
3892 1537 1513 808 734 470 341
PfoI T'CCnGG_A
Cuts at: 2955 3126 4287 4419 6494 2955
Size: 171 1161 132 2075 5756
Fragments arranged by size:
5756 2075 1161 171 132
PmeI GTTT'AAAC
Cuts at: 6599 6599
Size: 9295
PmlI CAC'GTG
Cuts at: 4978 4978
Size: 9295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3811 3843 7364 7396 8877 8909 3811
Size: 32 3521 32 1481 32 4197
Fragments arranged by size:
4197 3521 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 2516 3346 3808 4285 4931 4993 5315 5424
Size: 91 830 462 477 646 62 322 109
Cuts at: 5424 5455 5467 5767 6125 2425
Size: 31 12 300 358 5595
Fragments arranged by size:
5595 830 646 477 462 358 322 300
109 91 62 31 12
PshAI GACnn'nnGTC
Cuts at: 1613 3033 5862 1613
Size: 1420 2829 5046
Fragments arranged by size:
5046 2829 1420
PsiI TTA'TAA
Cuts at: 6865 6865
Size: 9295
PspOMI G'GGCC_C
Cuts at: 4746 5176 5338 6515 4746
Size: 430 162 1177 7526
Fragments arranged by size:
7526 1177 430 162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6073 6105 6073
Size: 32 9263
PstI C_TGCA'G
Cuts at: 830 2532 4632 6196 6225 6647 830
Size: 1702 2100 1564 29 422 3478
Fragments arranged by size:
3478 2100 1702 1564 422 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9295
PvuII CAG'CTG
Cuts at: 3382 3509 4132 4633 6220 3382
Size: 127 623 501 1587 6457
Fragments arranged by size:
6457 1587 623 501 127
SacI G_AGCT'C
Cuts at: 2446 5241 2446
Size: 2795 6500
SalI G'TCGA_C
Cuts at: 1936 6637 1936
Size: 4701 4594
SanDI GG'GwC_CC
Cuts at: 3346 4931 5424 5767 3346
Size: 1585 493 343 6874
Fragments arranged by size:
6874 1585 493 343
SapI GCTCTTCn'nnn_
Cuts at: 2392 3566 3664 4361 4592 2392
Size: 1174 98 697 231 7095
Fragments arranged by size:
7095 1174 697 231 98
SbfI CC_TGCA'GG
Cuts at: 6196 6647 6196
Size: 451 8844
ScaI AGT'ACT
Cuts at: 7543 9255 7543
Size: 1712 7583
SexAI A'CCwGG_T
Cuts at: 4004 5451 4004
Size: 1447 7848
SfcI C'TryA_G
Cuts at: 826 1045 2528 3170 3277 4628 5009 6192 6221
Size: 219 1483 642 107 1351 381 1183 29
Cuts at: 6221 6643 7778 8459 8650 826
Size: 422 1135 681 191 1471
Fragments arranged by size:
1483 1471 1351 1183 1135 681 642 422
381 219 191 107 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2807 2807
Size: 9295
SfoI GGC'GCC
Cuts at: 2359 2359
Size: 9295
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9295
SmaI CCC'GGG
Cuts at: 1872 2922 4337 4751 5321 6622 1872
Size: 1050 1415 414 570 1301 4545
Fragments arranged by size:
4545 1415 1301 1050 570 414
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3234 3866 4013 4772 5099 6136
Size: 197 926 1291 632 147 759 327 1037
Cuts at: 6136 6323 6338 6371 7399 8300 8562 820
Size: 187 15 33 1028 901 262 1553
Fragments arranged by size:
1553 1291 1037 1028 926 901 759 632
327 262 197 187 147 33 15
SnaBI TAC'GTA
Cuts at: 493 6592 7086 493
Size: 6099 494 2702
Fragments arranged by size:
6099 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9295
SphI G_CATG'C
Cuts at: 1132 6118 6653 1132
Size: 4986 535 3774
Fragments arranged by size:
4986 3774 535
SspI AAT'ATT
Cuts at: 5 52 3121 7219 5
Size: 47 3069 4098 2081
Fragments arranged by size:
4098 3069 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9295
StyI C'CwwG_G
Cuts at: 513 1065 2307 2400 3084 3391 3435 3603 4965
Size: 552 1242 93 684 307 44 168 1362
Cuts at: 4965 5383 5440 5685 5891 6379 6734 513
Size: 418 57 245 206 488 355 3074
Fragments arranged by size:
3074 1362 1242 684 552 488 418 355
307 245 206 168 93 57 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 5362 5495 5743 5831 6175 7322
Size: 1255 993 2789 133 248 88 344 1147
Cuts at: 7322 7339 7492 7677 8122 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2789 1498 1255 1147 993 445 344 248
185 153 133 88 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7541 9253 96
Size: 274 80 33 51 1443 5564 1712 138
Fragments arranged by size:
5564 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2361 2393 4269 4301 9255 9287 2361
Size: 32 1876 32 4954 32 2369
Fragments arranged by size:
4954 2369 1876 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9295
XbaI T'CTAG_A
Cuts at: 6631 6631
Size: 9295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4873 6053 4873
Size: 1180 8115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9295
XmaI C'CCGG_G
Cuts at: 1870 2920 4335 4749 5319 6620 1870
Size: 1050 1415 414 570 1301 4545
Fragments arranged by size:
4545 1415 1301 1050 570 414
XmnI GAAnn'nnTTC
Cuts at: 2709 3122 7424 2709
Size: 413 4302 4580
Fragments arranged by size:
4580 4302 413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5211 7103 276
Size: 53 83 186 936 3677 1892 2468
Fragments arranged by size:
3677 2468 1892 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI
RsrII SacII SrfI SwaI