Restriction map for
pFN21ASDA0375
(Circular) (Six-base) MAPSORT of: pFN21ASDA0375.seq Check: 3962 from: 1 to: 9277
pFN21ASDA0375 9277 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 2427 2111
Size: 316 8961
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 5199 7087 278
Size: 53 83 186 936 1966 1697 1888 2468
Fragments arranged by size:
2468 1966 1888 1697 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3462 5862 6620 1937
Size: 1525 2400 758 4594
Fragments arranged by size:
4594 2400 1525 758
Acc65I G'GTAC_C
Cuts at: 5835 6598 5835
Size: 763 8514
AclI AA'CG_TT
Cuts at: 1563 7404 7777 1563
Size: 5841 373 3063
Fragments arranged by size:
5841 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2857 4979 5153 5509 7340 8724 8992 1632
Size: 1225 2122 174 356 1831 1384 268 1917
Fragments arranged by size:
2122 1917 1831 1384 1225 356 268 174
AfeI AGC'GCT
Cuts at: 3506 3506
Size: 9277
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9080
AflIII A'CryG_T
Cuts at: 1203 8176 9153 1203
Size: 6973 977 1327
Fragments arranged by size:
6973 1327 977
AgeI A'CCGG_T
Cuts at: 8108 8108
Size: 9277
AhdI GACnn_n'nnGTC
Cuts at: 8006 8006
Size: 9277
AleI CACnn'nnGTG
Cuts at: 1166 3903 1166
Size: 2737 6540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 2701
Size: 32 9245
AlwNI CAG_nnn'CTG
Cuts at: 2589 2972 3152 3320 3428 3431 3974 5509 5614
Size: 383 180 168 108 3 543 1535 105
Cuts at: 5614 6385 7020 8592 2589
Size: 771 635 1572 3274
Fragments arranged by size:
3274 1572 1535 771 635 543 383 180
168 108 105 3
ApaI G_GGCC'C
Cuts at: 3440 3605 3778 4159 4395 5656 5672 5812 6117
Size: 165 173 381 236 1261 16 140 305
Cuts at: 6117 3440
Size: 6600
Fragments arranged by size:
6600 1261 381 305 236 173 165 140
16
ApaLI G'TGCA_C
Cuts at: 2081 7334 8490 2081
Size: 5253 1156 2868
Fragments arranged by size:
5253 2868 1156
ApoI r'AATT_y
Cuts at: 1493 6586 6812 6964 9073 1493
Size: 5093 226 152 2109 1697
Fragments arranged by size:
5093 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7831 160
Size: 872 6799 1606
Fragments arranged by size:
6799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9277
AvaI C'yCGr_G
Cuts at: 1870 1943 4152 4287 4635 5072 5649 5655 5811
Size: 73 2209 135 348 437 577 6 156
Cuts at: 5811 5973 6602 1870
Size: 162 629 4545
Fragments arranged by size:
4545 2209 629 577 437 348 162 156
135 73 6
AvrII C'CTAG_G
Cuts at: 2197 2197
Size: 9277
BamHI G'GATC_C
Cuts at: 3964 5068 6607 6974 3964
Size: 1104 1539 367 6267
Fragments arranged by size:
6267 1539 1104 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 2900 2975 4043 4304
Size: 325 217 627 792 321 75 1068 261
Cuts at: 4304 5720 5835 6508 6598 8053 618
Size: 1416 115 673 90 1455 1842
Fragments arranged by size:
1842 1455 1416 1068 792 673 627 325
321 261 217 115 90 75
BanII G_rGCy'C
Cuts at: 1391 2311 3440 3560 3605 3778 3992 4159 4395
Size: 920 1129 120 45 173 214 167 236
Cuts at: 4395 4598 5111 5178 5316 5656 5672 5792 5812
Size: 203 513 67 138 340 16 120 20
Cuts at: 5812 5855 6065 6117 6318 6596 1391
Size: 43 210 52 201 278 4072
Fragments arranged by size:
4072 1129 920 513 340 278 236 214
210 203 201 173 167 138 120 120
67 52 45 43 20 16
BbeI G_GCGC'C
Cuts at: 2979 2979
Size: 9277
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5415 928
Size: 971 3516 4790
Fragments arranged by size:
4790 3516 971
BbvCI CC'TCA_GC
Cuts at: 3488 4613 5997 6089 3488
Size: 1125 1384 92 6676
Fragments arranged by size:
6676 1384 1125 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7468 7502 1903
Size: 34 5531 34 3678
Fragments arranged by size:
5531 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2557 7169 8379 2557
Size: 4612 1210 3455
Fragments arranged by size:
4612 3455 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9277
BfrBI ATG'CAT
Cuts at: 2896 2896
Size: 9277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3261 3662 3829
Size: 107 122 71 631 605 1589 401 167
Cuts at: 3829 4191 4223 5667 5807 7888 136
Size: 362 32 1444 140 2081 1525
Fragments arranged by size:
2081 1589 1525 1444 631 605 401 362
167 140 122 107 71 32
BglII A'GATC_T
Cuts at: 9272 9272
Size: 9277
BlpI GC'TnA_GC
Cuts at: 3954 6251 6419 6694 3954
Size: 2297 168 275 6537
Fragments arranged by size:
6537 2297 275 168
Bme1580I G_kGCm'C
Cuts at: 1790 2085 3143 3440 3605 3778 3794 4159 4395
Size: 295 1058 297 165 173 16 365 236
Cuts at: 4395 5147 5656 5672 5812 6117 6513 7338 8494
Size: 752 509 16 140 305 396 825 1156
Cuts at: 8494 1790
Size: 2573
Fragments arranged by size:
2573 1156 1058 825 752 509 396 365
305 297 295 236 173 165 140 16
16
BmgBI CAC'GTC
Cuts at: 1318 5680 1318
Size: 4362 4915
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3578 3945 4665
Size: 416 53 83 399 24 2161 367 720
Cuts at: 4665 7008 7966 442
Size: 2343 958 1753
Fragments arranged by size:
2343 2161 1753 958 720 416 399 367
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2072 5472 6358 2072
Size: 3400 886 4991
Fragments arranged by size:
4991 3400 886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 2226 2325 2541 3705 3886 4920 5186
Size: 828 159 99 216 1164 181 1034 266
Cuts at: 5186 5396 5694 6186 6343 6483 7937 1239
Size: 210 298 492 157 140 1454 2579
Fragments arranged by size:
2579 1454 1164 1034 828 492 298 266
216 210 181 159 157 140 99
Bpu10I CC'TnA_GC
Cuts at: 2166 2755 3093 3488 4613 5791 5997 6089 2166
Size: 589 338 395 1125 1178 206 92 5354
Fragments arranged by size:
5354 1178 1125 589 395 338 206 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3470 3613 4224 4680 4688 5162 7402 8267 8565
Size: 143 611 456 8 474 2240 865 298
Cuts at: 8565 3470
Size: 4182
Fragments arranged by size:
4182 2240 865 611 474 456 298 143
8
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 4325 7940 882
Size: 617 546 2280 3615 2219
Fragments arranged by size:
3615 2280 2219 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 6523 6574 7068 493
Size: 711 5319 51 494 2702
Fragments arranged by size:
5319 2702 711 494 51
BsaBI GATnn'nnATC
Cuts at: 9271 9271
Size: 9277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2976 3499 5196 7084
Size: 53 83 186 936 1443 523 1697 1888
Cuts at: 7084 7466 8101 275
Size: 382 635 1451
Fragments arranged by size:
1888 1697 1451 1443 936 635 523 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4371 5367 7710 8108 8382 8529 8940 4371
Size: 996 2343 398 274 147 411 4708
Fragments arranged by size:
4708 2343 996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2644 2674 3098 3128 5369
Size: 30 165 30 1241 30 424 30 2241
Cuts at: 5369 5399 6523 6553 1178
Size: 30 1124 30 3902
Fragments arranged by size:
3902 2241 1241 1124 424 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2827 3718 5771 6032 6480 1186
Size: 1641 891 2053 261 448 3983
Fragments arranged by size:
3983 2053 1641 891 448 261
BseYI C'CCAG_C
Cuts at: 2170 2181 2403 3142 3338 3759 3991 4173 4252
Size: 11 222 739 196 421 232 182 79
Cuts at: 4252 4316 4394 4512 5089 6207 6346 6451 8480
Size: 64 78 118 577 1118 139 105 2029
Cuts at: 8480 8976 2170
Size: 496 2471
Fragments arranged by size:
2471 2029 1118 739 577 496 421 232
222 196 182 139 118 105 79 78
64 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 2238 5472 6398 1163
Size: 573 480 22 3234 926 4042
Fragments arranged by size:
4042 3234 926 573 480 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6727 7488 8516 1540
Size: 275 186 4726 761 1028 2301
Fragments arranged by size:
4726 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2141 4427 5316 5855 7338 7423 8494 2085
Size: 56 2286 889 539 1483 85 1071 2868
Fragments arranged by size:
2868 2286 1483 1071 889 539 85 56
BsmI GAATG_Cn'
Cuts at: 6788 6881 6788
Size: 93 9184
BsmBI CGTCTCn'nnnn_
Cuts at: 5670 5670
Size: 9277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2141 2311 3143 3440 3560 3605
Size: 399 295 56 170 832 297 120 45
Cuts at: 3605 3778 3794 3992 4159 4395 4427 4598 5111
Size: 173 16 198 167 236 32 171 513
Cuts at: 5111 5147 5178 5316 5656 5672 5792 5812 5855
Size: 36 31 138 340 16 120 20 43
Cuts at: 5855 6065 6117 6318 6513 6596 7338 7423 8494
Size: 210 52 201 195 83 742 85 1071
Cuts at: 8494 1391
Size: 2174
Fragments arranged by size:
2174 1071 832 742 513 399 340 297
295 236 210 201 198 195 173 171
170 167 138 120 120 85 83 56
52 45 43 36 32 31 20 16
16
BspEI T'CCGG_A
Cuts at: 4371 8940 4371
Size: 4569 4708
BspHI T'CATG_A
Cuts at: 7166 8896 7166
Size: 1730 7547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 2427 4901 4998 5622 6632 844
Size: 1267 316 2474 97 624 1010 3489
Fragments arranged by size:
3489 2474 1267 1010 624 316 97
BsrBI CCG'CTC
Cuts at: 1394 1823 5704 7164 1394
Size: 429 3881 1460 3507
Fragments arranged by size:
3881 3507 1460 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7772 7946 65
Size: 1183 6524 174 1396
Fragments arranged by size:
6524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2595 4560 4854 5550 5825 6404 7921
Size: 110 785 1965 294 696 275 579 1517
Cuts at: 7921 8108 1700
Size: 187 2869
Fragments arranged by size:
2869 1965 1517 785 696 579 294 275
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9277
BssSI C'ACGA_G
Cuts at: 5898 7337 8349 5898
Size: 1439 1012 6826
Fragments arranged by size:
6826 1439 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3428 5554 794
Size: 2634 2126 4517
Fragments arranged by size:
4517 2634 2126
BstBI TT'CG_AA
Cuts at: 2007 8081 2007
Size: 6074 3203
BstEII G'GTnAC_C
Cuts at: 1184 2366 2866 3643 1184
Size: 1182 500 777 6818
Fragments arranged by size:
6818 1182 777 500
BstXI CCAn_nnnn'nTGG
Cuts at: 2423 3826 4141 2423
Size: 1403 315 7559
Fragments arranged by size:
7559 1403 315
BstYI r'GATC_y
Cuts at: 1972 2989 3964 5068 6607 6974 7359 7376 8828
Size: 1017 975 1104 1539 367 385 17 1452
Cuts at: 8828 8914 8926 9272 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1539 1452 1104 1017 975 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3463 3463
Size: 9277
Bsu36I CC'TnA_GG
Cuts at: 4261 4261
Size: 9277
BtgI C'CryG_G
Cuts at: 513 1065 2022 2495 2817 3612 4469 4567 5225
Size: 552 957 473 322 795 857 98 658
Cuts at: 5225 6487 513
Size: 1262 3303
Fragments arranged by size:
3303 1262 957 857 795 658 552 473
322 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4329 5011 487
Size: 683 56 3103 682 4753
Fragments arranged by size:
4753 3103 683 682 56
BtsI GCAGTG_nn'
Cuts at: 2083 2111 2579 2587 2665 2767 3153 3408 4094
Size: 28 468 8 78 102 386 255 686
Cuts at: 4094 4878 6793 7587 7607 2083
Size: 784 1915 794 20 3753
Fragments arranged by size:
3753 1915 794 784 686 468 386 255
102 78 28 20 8
ClaI AT'CG_AT
Cuts at: 1554 5868 1554
Size: 4314 4963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3430 3465 4731 4766 529
Size: 35 2866 35 1266 35 5040
Fragments arranged by size:
5040 2866 1266 35 35 35
DraI TTT'AAA
Cuts at: 6581 6581
Size: 9277
DraIII CAC_nnn'GTG
Cuts at: 3131 3710 4422 3131
Size: 579 712 7986
Fragments arranged by size:
7986 712 579
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8284 809
Size: 507 6968 1802
Fragments arranged by size:
6968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2431 2597 3195
Size: 54 1425 50 275 18 601 166 598
Cuts at: 3195 3615 3663 4143 4340 4659 4864 6081 6399
Size: 420 48 480 197 319 205 1217 318
Cuts at: 6399 6724 7613 9242 8
Size: 325 889 1629 43
Fragments arranged by size:
1629 1425 1217 889 601 598 480 420
325 319 318 275 205 197 166 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6724 1537
Size: 275 4912 4090
Fragments arranged by size:
4912 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2290 2953 3091 3271 4411 5478 5733 5739
Size: 937 663 138 180 1140 1067 255 6
Cuts at: 5739 5748 5760 7207 1353
Size: 9 12 1447 3423
Fragments arranged by size:
3423 1447 1140 1067 937 663 255 180
138 12 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3539 7864 8238 8384 387
Size: 1064 138 1950 4325 374 146 1280
Fragments arranged by size:
4325 1950 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5314 5853 5314
Size: 539 8738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2226 2325 2541 2857 3705 3886
Size: 393 435 159 99 216 316 848 181
Cuts at: 3886 4920 4979 5153 5186 5396 5509 5694 6186
Size: 1034 59 174 33 210 113 185 492
Cuts at: 6186 6343 6483 7340 7937 8724 8992 1239
Size: 157 140 857 597 787 268 1524
Fragments arranged by size:
1524 1034 857 848 787 597 492 435
393 316 268 216 210 185 181 174
159 157 140 113 99 59 33
EcoO109I rG'GnC_Cy
Cuts at: 2300 2642 3317 3335 3437 3601 3602 3774 3775
Size: 342 675 18 102 164 1 172 1
Cuts at: 3775 3893 3944 3971 4156 4909 5272 5611 5668
Size: 118 51 27 185 753 363 339 57
Cuts at: 5668 5669 5951 6022 6113 6147 2300
Size: 1 282 71 91 34 5430
Fragments arranged by size:
5430 753 675 363 342 339 282 185
172 164 118 102 91 71 57 51
34 27 18 1 1 1
EcoRI G'AATT_C
Cuts at: 6586 6964 6586
Size: 378 8899
FspI TGC'GCA
Cuts at: 7783 7783
Size: 9277
HaeII r_GCGC'y
Cuts at: 1678 2979 3508 8424 1678
Size: 1301 529 4916 2531
Fragments arranged by size:
4916 2531 1301 529
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 4596 4628 4757
Size: 32 135 32 697 32 2295 32 129
Cuts at: 4757 4789 4952 4984 6082 6114 6523 6555 7918
Size: 32 163 32 1098 32 409 32 1363
Cuts at: 7918 7950 7992 8024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2295 1363 1098 697 409 163 135
129 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5023 6621 6867 669
Size: 988 281 3085 1598 246 3079
Fragments arranged by size:
3085 3079 1598 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4579 748
Size: 3831 5446
HpaI GTT'AAC
Cuts at: 5023 6867 5023
Size: 1844 7433
KasI G'GCGC_C
Cuts at: 2975 2975
Size: 9277
KpnI G_GTAC'C
Cuts at: 5839 6602 5839
Size: 763 8514
MfeI C'AATT_G
Cuts at: 6876 6876
Size: 9277
MluI A'CGCG_T
Cuts at: 9153 9153
Size: 9277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6298 6572 8391 8575 1305
Size: 4993 274 1819 184 2007
Fragments arranged by size:
4993 2007 1819 274 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2433 3197 3665 4145 4661
Size: 54 1425 343 601 764 468 480 516
Cuts at: 4661 4866 6401 10
Size: 205 1535 2886
Fragments arranged by size:
2886 1535 1425 764 601 516 480 468
343 205 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 3903 5030 7235 7594 7753 518
Size: 648 1255 1482 1127 2205 359 159 2042
Fragments arranged by size:
2205 2042 1482 1255 1127 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2464 2775 2807 3428 3958 4141 4940 5578
Size: 858 311 32 621 530 183 799 638
Cuts at: 5578 5644 6119 6211 6418 6693 7370 8518 8763
Size: 66 475 92 207 275 677 1148 245
Cuts at: 8763 1606
Size: 2120
Fragments arranged by size:
2120 1148 858 799 677 638 621 530
475 311 275 245 207 183 92 66
32
NaeI GCC'GGC
Cuts at: 2597 4856 5827 2597
Size: 2259 971 6047
Fragments arranged by size:
6047 2259 971
NarI GG'CG_CC
Cuts at: 2976 2976
Size: 9277
NcoI C'CATG_G
Cuts at: 513 1065 2022 2495 2817 3612 4469 4567 5225
Size: 552 957 473 322 795 857 98 658
Cuts at: 5225 513
Size: 4565
Fragments arranged by size:
4565 957 857 795 658 552 473 322
98
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9277
NgoMIV G'CCGG_C
Cuts at: 2595 4854 5825 2595
Size: 2259 971 6047
Fragments arranged by size:
6047 2259 971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9277
NotI GC'GGCC_GC
Cuts at: 6724 6724
Size: 9277
NsiI A_TGCA'T
Cuts at: 2898 2898
Size: 9277
NspI r_CATG'y
Cuts at: 1132 1229 5224 6635 8180 1132
Size: 97 3995 1411 1545 2229
Fragments arranged by size:
3995 2229 1545 1411 97
PasI CC'CwG_GG
Cuts at: 1348 3983 4351 5436 6025 1348
Size: 2635 368 1085 589 4600
Fragments arranged by size:
4600 2635 1085 589 368
PciI A'CATG_T
Cuts at: 8176 8176
Size: 9277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2363 2474 2818 3320 6280 6397 6554 1108
Size: 1255 111 344 502 2960 117 157 3831
Fragments arranged by size:
3831 2960 1255 502 344 157 117 111
PfoI T'CCnGG_A
Cuts at: 2242 4905 2242
Size: 2663 6614
PmeI GTTT'AAAC
Cuts at: 6581 6581
Size: 9277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7346 7378 8859 8891 7346
Size: 32 1481 32 7732
Fragments arranged by size:
7732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2642 3893 4909 5272 5611 5951 2642
Size: 1251 1016 363 339 340 5968
Fragments arranged by size:
5968 1251 1016 363 340 339
PshAI GACnn'nnGTC
Cuts at: 1613 3861 1613
Size: 2248 7029
PsiI TTA'TAA
Cuts at: 6847 6847
Size: 9277
PspOMI G'GGCC_C
Cuts at: 3436 3601 3774 4155 4391 5652 5668 5808 6113
Size: 165 173 381 236 1261 16 140 305
Cuts at: 6113 3436
Size: 6600
Fragments arranged by size:
6600 1261 381 305 236 173 165 140
16
PstI C_TGCA'G
Cuts at: 830 2340 2780 2942 2960 2997 3534 3610 4199
Size: 1510 440 162 18 37 537 76 589
Cuts at: 4199 5643 6333 6485 6629 830
Size: 1444 690 152 144 3478
Fragments arranged by size:
3478 1510 1444 690 589 537 440 162
152 144 76 37 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9277
PvuII CAG'CTG
Cuts at: 2464 2807 3428 3958 4141 4940 5644 6418 2464
Size: 343 621 530 183 799 704 774 5323
Fragments arranged by size:
5323 799 774 704 621 530 343 183
SacI G_AGCT'C
Cuts at: 5316 5855 5316
Size: 539 8738
SalI G'TCGA_C
Cuts at: 1936 6619 1936
Size: 4683 4594
SanDI GG'GwC_CC
Cuts at: 3893 3893
Size: 9277
SapI GCTCTTCn'nnn_
Cuts at: 3271 5478 3271
Size: 2207 7070
SbfI CC_TGCA'GG
Cuts at: 6333 6629 6333
Size: 296 8981
ScaI AGT'ACT
Cuts at: 5138 7525 9237 5138
Size: 2387 1712 5178
Fragments arranged by size:
5178 2387 1712
SexAI A'CCwGG_T
Cuts at: 2644 4744 5124 2644
Size: 2100 380 6797
Fragments arranged by size:
6797 2100 380
SfcI C'TryA_G
Cuts at: 826 1045 2262 2336 2776 2938 2956 2993 3530
Size: 219 1217 74 440 162 18 37 537
Cuts at: 3530 3606 3799 4069 4180 4195 5501 5639 6329
Size: 76 193 270 111 15 1306 138 690
Cuts at: 6329 6481 6625 7760 8441 8632 826
Size: 152 144 1135 681 191 1471
Fragments arranged by size:
1471 1306 1217 1135 690 681 537 440
270 219 193 191 162 152 144 138
111 76 74 37 18 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 5667 5667
Size: 9277
SfoI GGC'GCC
Cuts at: 2977 2977
Size: 9277
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9277
SmaI CCC'GGG
Cuts at: 1872 4154 4637 5074 5651 5657 6604 1872
Size: 2282 483 437 577 6 947 4545
Fragments arranged by size:
4545 2282 947 577 483 437 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3449 3592 4239 4695 4703 5177
Size: 197 926 1506 143 647 456 8 474
Cuts at: 5177 7381 8282 8544 820
Size: 2204 901 262 1553
Fragments arranged by size:
2204 1553 1506 926 901 647 474 456
262 197 143 8
SnaBI TAC'GTA
Cuts at: 493 6574 7068 493
Size: 6081 494 2702
Fragments arranged by size:
6081 2702 494
SpeI A'CTAG_T
Cuts at: 152 6277 152
Size: 6125 3152
SphI G_CATG'C
Cuts at: 1132 6635 1132
Size: 5503 3774
SrfI GCCC'GGGC
Cuts at: 4154 5651 4154
Size: 1497 7780
SspI AAT'ATT
Cuts at: 5 52 7201 5
Size: 47 7149 2081
Fragments arranged by size:
7149 2081 47
StuI AGG'CCT
Cuts at: 1517 5183 6002 1517
Size: 3666 819 4792
Fragments arranged by size:
4792 3666 819
StyI C'CwwG_G
Cuts at: 513 1065 2022 2197 2495 2559 2732 2817 3612
Size: 552 957 175 298 64 173 85 795
Cuts at: 3612 3666 4146 4469 4567 5225 5268 5695 6318
Size: 54 480 323 98 658 43 427 623
Cuts at: 6318 6716 513
Size: 398 3074
Fragments arranged by size:
3074 957 795 658 623 552 480 427
398 323 298 175 173 98 85 64
54 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 2339 2417 3431 3649 3760 3832
Size: 1255 615 144 78 1014 218 111 72
Cuts at: 3832 3893 4782 5708 7304 7321 7474 7659 8104
Size: 61 889 926 1596 17 153 185 445
Cuts at: 8104 325
Size: 1498
Fragments arranged by size:
1596 1498 1255 1014 926 889 615 445
218 185 153 144 111 78 72 61
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5136 5188 7523
Size: 274 80 33 51 1443 3159 52 2335
Cuts at: 7523 9235 96
Size: 1712 138
Fragments arranged by size:
3159 2335 1712 1443 274 138 80 52
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2644 2676 4086 4118 4154 4186 9237 9269 2644
Size: 32 1410 32 36 32 5051 32 2652
Fragments arranged by size:
5051 2652 1410 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9277
XbaI T'CTAG_A
Cuts at: 6613 6613
Size: 9277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2969 3867 5641 5669 2969
Size: 898 1774 28 6577
Fragments arranged by size:
6577 1774 898 28
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9277
XmaI C'CCGG_G
Cuts at: 1870 4152 4635 5072 5649 5655 6602 1870
Size: 2282 483 437 577 6 947 4545
Fragments arranged by size:
4545 2282 947 577 483 437 6
XmnI GAAnn'nnTTC
Cuts at: 5098 7406 5098
Size: 2308 6969
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 5197 7085 276
Size: 53 83 186 936 1966 1697 1888 2468
Fragments arranged by size:
2468 1966 1888 1697 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BsiWI EcoNI EcoRV FalI FseI FspAI
NruI PacI PmlI PspXI PsrI RsrII SacII SwaI