Restriction map for
pFN21ASDA0372
(Circular) (Six-base) MAPSORT of: pFN21ASDA0372.seq Check: 5374 from: 1 to: 9421
pFN21ASDA0372 9421 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7231 278
Size: 53 83 186 936 5695 2468
Fragments arranged by size:
5695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4101 4343 6301 6764 1937
Size: 2164 242 1958 463 4594
Fragments arranged by size:
4594 2164 1958 463 242
Acc65I G'GTAC_C
Cuts at: 6594 6742 6594
Size: 148 9273
AclI AA'CG_TT
Cuts at: 1563 7548 7921 1563
Size: 5985 373 3063
Fragments arranged by size:
5985 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3041 3111 3303 3491 4756 4824 5489 6072
Size: 1409 70 192 188 1265 68 665 583
Cuts at: 6072 7484 8868 9136 1632
Size: 1412 1384 268 1917
Fragments arranged by size:
1917 1412 1409 1384 1265 665 583 268
192 188 70 68
AflII C'TTAA_G
Cuts at: 820 1017 3847 4430 820
Size: 197 2830 583 5811
Fragments arranged by size:
5811 2830 583 197
AflIII A'CryG_T
Cuts at: 1203 6244 8320 9297 1203
Size: 5041 2076 977 1327
Fragments arranged by size:
5041 2076 1327 977
AgeI A'CCGG_T
Cuts at: 8252 8252
Size: 9421
AhdI GACnn_n'nnGTC
Cuts at: 6528 8150 6528
Size: 1622 7799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4859 1184
Size: 3675 5746
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3174 3205 3206 3237 3896 3928 3174
Size: 31 1 31 659 32 8667
Fragments arranged by size:
8667 659 32 31 31 1
AlwNI CAG_nnn'CTG
Cuts at: 2521 3776 6173 6238 7164 8736 2521
Size: 1255 2397 65 926 1572 3206
Fragments arranged by size:
3206 2397 1572 1255 926 65
ApaLI G'TGCA_C
Cuts at: 7478 8634 7478
Size: 1156 8265
ApoI r'AATT_y
Cuts at: 1493 2663 4947 5966 6076 6730 6956 7108 9217
Size: 1170 2284 1019 110 654 226 152 2109
Cuts at: 9217 1493
Size: 1697
Fragments arranged by size:
2284 2109 1697 1170 1019 654 226 152
110
AseI AT'TA_AT
Cuts at: 160 1032 4374 4599 7975 160
Size: 872 3342 225 3376 1606
Fragments arranged by size:
3376 3342 1606 872 225
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9421
AvaI C'yCGr_G
Cuts at: 1870 1943 5622 6746 1870
Size: 73 3679 1124 4545
Fragments arranged by size:
4545 3679 1124 73
AvrII C'CTAG_G
Cuts at: 3955 3955
Size: 9421
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3363 3396 6233 6266 6578 6611 3363
Size: 33 2837 33 312 33 6173
Fragments arranged by size:
6173 2837 312 33 33 33
BamHI G'GATC_C
Cuts at: 6751 7118 6751
Size: 367 9054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5505 6594 6742 8197 618
Size: 325 217 627 3718 1089 148 1455 1842
Fragments arranged by size:
3718 1842 1455 1089 627 325 217 148
BanII G_rGCy'C
Cuts at: 1391 3361 3562 4296 6740 1391
Size: 1970 201 734 2444 4072
Fragments arranged by size:
4072 2444 1970 734 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 3071 3258 5070 5184 928
Size: 971 149 1023 187 1812 114 5165
Fragments arranged by size:
5165 1812 1023 971 187 149 114
BbvCI CC'TCA_GC
Cuts at: 4737 4737
Size: 9421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7612 7646 1903
Size: 34 5675 34 3678
Fragments arranged by size:
5675 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2745 3481 6573 7313 8523 2745
Size: 736 3092 740 1210 3643
Fragments arranged by size:
3643 3092 1210 740 736
BclI T'GATC_A
Cuts at: 1548 2191 3151 3993 6094 6403 1548
Size: 643 960 842 2101 309 4566
Fragments arranged by size:
4566 2101 960 842 643 309
BfrBI ATG'CAT
Cuts at: 2980 4798 5027 2980
Size: 1818 229 7374
Fragments arranged by size:
7374 1818 229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2850 3299 8032
Size: 107 122 71 631 605 1178 449 4733
Cuts at: 8032 136
Size: 1525
Fragments arranged by size:
4733 1525 1178 631 605 449 122 107
71
BglII A'GATC_T
Cuts at: 9416 9416
Size: 9421
BlpI GC'TnA_GC
Cuts at: 4560 6838 4560
Size: 2278 7143
Bme1580I G_kGCm'C
Cuts at: 1790 7482 8638 1790
Size: 5692 1156 2573
Fragments arranged by size:
5692 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 6643 1318
Size: 5325 4096
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2600 7152 8110
Size: 416 53 83 399 24 1183 4552 958
Cuts at: 8110 442
Size: 1753
Fragments arranged by size:
4552 1753 1183 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4181 1056
Size: 3125 6296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3633 4463 4877 5805 6167 8081 1239
Size: 2394 830 414 928 362 1914 2579
Fragments arranged by size:
2579 2394 1914 928 830 414 362
Bpu10I CC'TnA_GC
Cuts at: 2298 2854 3725 4737 2298
Size: 556 871 1012 6982
Fragments arranged by size:
6982 1012 871 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3317 3383 3750 6328 7546 8411 8709 3317
Size: 66 367 2578 1218 865 298 4029
Fragments arranged by size:
4029 2578 1218 865 367 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5616 6009 8084 882
Size: 617 4117 393 2075 2219
Fragments arranged by size:
4117 2219 2075 617 393
BsaAI yAC'GTr
Cuts at: 493 1204 6718 7212 493
Size: 711 5514 494 2702
Fragments arranged by size:
5514 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2714 9415 2714
Size: 6701 2720
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6529 7228 7610 8245
Size: 53 83 186 936 4996 699 382 635
Cuts at: 8245 275
Size: 1451
Fragments arranged by size:
4996 1451 936 699 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7854 8252 8526 8673 9084 7854
Size: 398 274 147 411 8191
Fragments arranged by size:
8191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5652 5682 1178
Size: 30 165 30 4249 30 4917
Fragments arranged by size:
4917 4249 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3870 4286 1186
Size: 2684 416 6321
Fragments arranged by size:
6321 2684 416
BseYI C'CCAG_C
Cuts at: 4181 4963 5843 8624 9120 4181
Size: 782 880 2781 496 4482
Fragments arranged by size:
4482 2781 880 782 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2493 2920 4819 5769 6052 6570 1163
Size: 573 757 427 1899 950 283 518 4014
Fragments arranged by size:
4014 1899 950 757 573 518 427 283
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6871 7632 8660 1540
Size: 275 186 4870 761 1028 2301
Fragments arranged by size:
4870 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3361 3562 4141 4296 5413 7482 7567 8638 3361
Size: 201 579 155 1117 2069 85 1071 4144
Fragments arranged by size:
4144 2069 1117 1071 579 201 155 85
BsiWI C'GTAC_G
Cuts at: 3143 3143
Size: 9421
BsmI GAATG_Cn'
Cuts at: 2590 3760 4049 4513 4906 6932 7025 2590
Size: 1170 289 464 393 2026 93 4986
Fragments arranged by size:
4986 2026 1170 464 393 289 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3528 3528
Size: 9421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3361 3562 4141 4296 5413 6740 7482
Size: 399 1571 201 579 155 1117 1327 742
Cuts at: 7482 7567 8638 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1571 1327 1117 1071 742 579 399
201 155 85
BspEI T'CCGG_A
Cuts at: 9084 9084
Size: 9421
BspHI T'CATG_A
Cuts at: 2671 4663 5208 7310 9040 2671
Size: 1992 545 2102 1730 3052
Fragments arranged by size:
3052 2102 1992 1730 545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6776 844
Size: 5932 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 7308 1394
Size: 429 5485 3507
Fragments arranged by size:
5485 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4841 5348 5430 7916 8090 65
Size: 1183 3593 507 82 2486 174 1396
Fragments arranged by size:
3593 2486 1396 1183 507 174 82
BsrFI r'CCGG_y
Cuts at: 1700 1810 8065 8252 1700
Size: 110 6255 187 2869
Fragments arranged by size:
6255 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2728 5724 96
Size: 2632 2996 3793
Fragments arranged by size:
3793 2996 2632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9421
BssSI C'ACGA_G
Cuts at: 3561 7481 8493 3561
Size: 3920 1012 4489
Fragments arranged by size:
4489 3920 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5024 5639 6368 6584 794
Size: 4230 615 729 216 3631
Fragments arranged by size:
4230 3631 729 615 216
BstBI TT'CG_AA
Cuts at: 2007 8225 2007
Size: 6218 3203
BstEII G'GTnAC_C
Cuts at: 1184 5594 1184
Size: 4410 5011
BstYI r'GATC_y
Cuts at: 1972 2335 2392 4765 6751 7118 7503 7520 8972
Size: 363 57 2373 1986 367 385 17 1452
Cuts at: 8972 9058 9070 9416 1972
Size: 86 12 346 1977
Fragments arranged by size:
2373 1986 1977 1452 385 367 363 346
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4761 513
Size: 552 3696 5173
Fragments arranged by size:
5173 3696 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8682
Fragments arranged by size:
8682 683 56
BtsI GCAGTG_nn'
Cuts at: 3777 4479 5551 5727 6239 6937 7731 7751 3777
Size: 702 1072 176 512 698 794 20 5447
Fragments arranged by size:
5447 1072 794 702 698 512 176 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9386
DraI TTT'AAA
Cuts at: 4029 5359 6081 6725 4029
Size: 1330 722 644 6725
Fragments arranged by size:
6725 1330 722 644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8428 809
Size: 507 7112 1802
Fragments arranged by size:
7112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6410 6868 7757
Size: 54 1425 50 275 18 4580 458 889
Cuts at: 7757 9386 8
Size: 1629 43
Fragments arranged by size:
4580 1629 1425 889 458 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6868 1537
Size: 275 5056 4090
Fragments arranged by size:
5056 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 3130 3268 3423 4775 7351 1353
Size: 1125 652 138 155 1352 2576 3423
Fragments arranged by size:
3423 2576 1352 1125 652 155 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8008 8382 8528 387
Size: 1064 138 6419 374 146 1280
Fragments arranged by size:
6419 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3359 3560 4294 3359
Size: 201 734 8486
Fragments arranged by size:
8486 734 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3041 3111 3303 3491 3633 4463 4756
Size: 393 1409 70 192 188 142 830 293
Cuts at: 4756 4824 4877 5489 5805 6072 6167 7484 8081
Size: 68 53 612 316 267 95 1317 597
Cuts at: 8081 8868 9136 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1409 1317 830 787 612 597 393
316 293 268 267 192 188 142 95
70 68 53
EcoNI CCTnn'n_nnAGG
Cuts at: 3724 3724
Size: 9421
EcoRI G'AATT_C
Cuts at: 4947 6730 7108 4947
Size: 1783 378 7260
Fragments arranged by size:
7260 1783 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2323 3017 5471 6472 2323
Size: 694 2454 1001 5272
Fragments arranged by size:
5272 2454 1001 694
FspI TGC'GCA
Cuts at: 7927 7927
Size: 9421
HaeII r_GCGC'y
Cuts at: 1678 8568 1678
Size: 6890 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2055 2087 3107 3139 3245
Size: 32 135 32 483 32 1020 32 106
Cuts at: 3245 3277 3593 3625 6514 6546 8062 8094 8136
Size: 32 316 32 2889 32 1516 32 42
Cuts at: 8136 8168 1373
Size: 32 2626
Fragments arranged by size:
2889 2626 1516 1020 483 316 135 106
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 3628 5157 6765 7011 669
Size: 988 281 415 1275 1529 1608 246 3079
Fragments arranged by size:
3079 1608 1529 1275 988 415 281 246
HindIII A'AGCT_T
Cuts at: 748 2094 3199 6449 6483 748
Size: 1346 1105 3250 34 3686
Fragments arranged by size:
3686 3250 1346 1105 34
HpaI GTT'AAC
Cuts at: 7011 7011
Size: 9421
KpnI G_GTAC'C
Cuts at: 6598 6746 6598
Size: 148 9273
MfeI C'AATT_G
Cuts at: 2561 3817 6305 7020 2561
Size: 1256 2488 715 4962
Fragments arranged by size:
4962 2488 1256 715
MluI A'CGCG_T
Cuts at: 9297 9297
Size: 9421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4726 6231 6308 8535 8719 1305
Size: 3421 1505 77 2227 184 2007
Fragments arranged by size:
3421 2227 2007 1505 184 77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6412 10
Size: 54 1425 343 4580 3019
Fragments arranged by size:
4580 3019 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 5868 6612 7379 7738 7897 518
Size: 648 1126 3576 744 767 359 159 2042
Fragments arranged by size:
3576 2042 1126 767 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2174 6837 7514 8662 8907 1606
Size: 568 4663 677 1148 245 2120
Fragments arranged by size:
4663 2120 1148 677 568 245
NcoI C'CATG_G
Cuts at: 513 1065 4761 513
Size: 552 3696 5173
Fragments arranged by size:
5173 3696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9421
NheI G'CTAG_C
Cuts at: 1052 4177 1052
Size: 3125 6296
NotI GC'GGCC_GC
Cuts at: 6868 6868
Size: 9421
NsiI A_TGCA'T
Cuts at: 2982 4800 5029 2982
Size: 1818 229 7374
Fragments arranged by size:
7374 1818 229
NspI r_CATG'y
Cuts at: 1132 1229 4530 6248 6779 8324 1132
Size: 97 3301 1718 531 1545 2229
Fragments arranged by size:
3301 2229 1718 1545 531 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9421
PciI A'CATG_T
Cuts at: 6244 8320 6244
Size: 2076 7345
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9421
PfoI T'CCnGG_A
Cuts at: 2331 6144 2331
Size: 3813 5608
PmeI GTTT'AAAC
Cuts at: 6725 6725
Size: 9421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7490 7522 9003 9035 7490
Size: 32 1481 32 7876
Fragments arranged by size:
7876 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9421
PsiI TTA'TAA
Cuts at: 5223 6991 5223
Size: 1768 7653
PstI C_TGCA'G
Cuts at: 830 2173 3622 5062 6280 6773 830
Size: 1343 1449 1440 1218 493 3478
Fragments arranged by size:
3478 1449 1440 1343 1218 493
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9421
PvuII CAG'CTG
Cuts at: 2174 2174
Size: 9421
SacI G_AGCT'C
Cuts at: 3361 3562 4296 3361
Size: 201 734 8486
Fragments arranged by size:
8486 734 201
SalI G'TCGA_C
Cuts at: 1936 6763 1936
Size: 4827 4594
SapI GCTCTTCn'nnn_
Cuts at: 2478 2478
Size: 9421
SbfI CC_TGCA'GG
Cuts at: 6773 6773
Size: 9421
ScaI AGT'ACT
Cuts at: 2631 7669 9381 2631
Size: 5038 1712 2671
Fragments arranged by size:
5038 2671 1712
SfcI C'TryA_G
Cuts at: 826 1045 2169 3618 3975 4142 4782 5058 6276
Size: 219 1124 1449 357 167 640 276 1218
Cuts at: 6276 6769 7904 8585 8776 826
Size: 493 1135 681 191 1471
Fragments arranged by size:
1471 1449 1218 1135 1124 681 640 493
357 276 219 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9421
SmaI CCC'GGG
Cuts at: 1872 6748 1872
Size: 4876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3296 3362 3765 3847 4430 5622
Size: 197 926 1353 66 403 82 583 1192
Cuts at: 5622 6343 7525 8426 8688 820
Size: 721 1182 901 262 1553
Fragments arranged by size:
1553 1353 1192 1182 926 901 721 583
403 262 197 82 66
SnaBI TAC'GTA
Cuts at: 493 6718 7212 493
Size: 6225 494 2702
Fragments arranged by size:
6225 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9421
SphI G_CATG'C
Cuts at: 1132 6779 1132
Size: 5647 3774
SspI AAT'ATT
Cuts at: 5 52 4127 7345 5
Size: 47 4075 3218 2081
Fragments arranged by size:
4075 3218 2081 47
StuI AGG'CCT
Cuts at: 1517 2696 2844 6443 1517
Size: 1179 148 3599 4495
Fragments arranged by size:
4495 3599 1179 148
StyI C'CwwG_G
Cuts at: 513 1065 3955 4303 4366 4761 5536 6259 6860
Size: 552 2890 348 63 395 775 723 601
Cuts at: 6860 513
Size: 3074
Fragments arranged by size:
3074 2890 775 723 601 552 395 348
63
SwaI ATTT'AAAT
Cuts at: 5359 5359
Size: 9421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3413 4028 7448 7465 7618 7803 8248
Size: 1255 1833 615 3420 17 153 185 445
Cuts at: 8248 325
Size: 1498
Fragments arranged by size:
3420 1833 1498 1255 615 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2531 2629 2728
Size: 274 80 33 51 1443 554 98 99
Cuts at: 2728 4881 5724 6175 7667 9379 96
Size: 2153 843 451 1492 1712 138
Fragments arranged by size:
2153 1712 1492 1443 843 554 451 274
138 99 98 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9381 9413 9381
Size: 32 9389
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9421
XbaI T'CTAG_A
Cuts at: 6757 6757
Size: 9421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6231 6231
Size: 9421
XhoI C'TCGA_G
Cuts at: 1943 5622 1943
Size: 3679 5742
XmaI C'CCGG_G
Cuts at: 1870 6746 1870
Size: 4876 4545
XmnI GAAnn'nnTTC
Cuts at: 2487 2997 3138 3255 3881 4947 5394 5758 7550
Size: 510 141 117 626 1066 447 364 1792
Cuts at: 7550 2487
Size: 4358
Fragments arranged by size:
4358 1792 1066 626 510 447 364 141
117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7229 276
Size: 53 83 186 936 5695 2468
Fragments arranged by size:
5695 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BplI BstXI BstZ17I
Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI