Restriction map for
pFN21ASDA0360
(Circular) (Six-base) MAPSORT of: pFN21ASDA0360.seq Check: 7243 from: 1 to: 7750
pFN21ASDA0360 7750 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2629 2629
Size: 7750
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5560 278
Size: 53 83 186 936 4024 2468
Fragments arranged by size:
4024 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5093 1937
Size: 3156 4594
Acc65I G'GTAC_C
Cuts at: 5071 5071
Size: 7750
AclI AA'CG_TT
Cuts at: 1563 5877 6250 1563
Size: 4314 373 3063
Fragments arranged by size:
4314 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 2731 2799 3086 4221 5813 7197 7465
Size: 738 361 68 287 1135 1592 1384 268
Cuts at: 7465 1632
Size: 1917
Fragments arranged by size:
1917 1592 1384 1135 738 361 287 268
68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7553
AflIII A'CryG_T
Cuts at: 1203 3307 6649 7626 1203
Size: 2104 3342 977 1327
Fragments arranged by size:
3342 2104 1327 977
AgeI A'CCGG_T
Cuts at: 6581 6581
Size: 7750
AhdI GACnn_n'nnGTC
Cuts at: 6479 6479
Size: 7750
AleI CACnn'nnGTG
Cuts at: 1166 3210 4682 1166
Size: 2044 1472 4234
Fragments arranged by size:
4234 2044 1472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2657 3386 1184
Size: 1473 729 5548
Fragments arranged by size:
5548 1473 729
AlwNI CAG_nnn'CTG
Cuts at: 2327 2423 2644 2714 3805 5493 7065 2327
Size: 96 221 70 1091 1688 1572 3012
Fragments arranged by size:
3012 1688 1572 1091 221 96 70
ApaI G_GGCC'C
Cuts at: 2363 3958 2363
Size: 1595 6155
ApaLI G'TGCA_C
Cuts at: 4047 5807 6963 4047
Size: 1760 1156 4834
Fragments arranged by size:
4834 1760 1156
ApoI r'AATT_y
Cuts at: 1493 2165 4093 5059 5285 5437 7546 1493
Size: 672 1928 966 226 152 2109 1697
Fragments arranged by size:
2109 1928 1697 966 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6304 160
Size: 872 5272 1606
Fragments arranged by size:
5272 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7750
AvaI C'yCGr_G
Cuts at: 1870 1943 3030 3951 4083 5075 1870
Size: 73 1087 921 132 992 4545
Fragments arranged by size:
4545 1087 992 921 132 73
BamHI G'GATC_C
Cuts at: 2347 5080 5447 2347
Size: 2733 367 4650
Fragments arranged by size:
4650 2733 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 5071 6526 618
Size: 325 217 627 888 2396 1455 1842
Fragments arranged by size:
2396 1842 1455 888 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2363 3063 3623 3765 3865 3958 5007 5069
Size: 972 700 560 142 100 93 1049 62
Cuts at: 5069 1391
Size: 4072
Fragments arranged by size:
4072 1049 972 700 560 142 100 93
62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2376 4518 4820 928
Size: 971 477 2142 302 3858
Fragments arranged by size:
3858 2142 971 477 302
BbvCI CC'TCA_GC
Cuts at: 3847 3895 4569 3847
Size: 48 674 7028
Fragments arranged by size:
7028 674 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5941 5975 1903
Size: 34 4004 34 3678
Fragments arranged by size:
4004 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5642 6852 5642
Size: 1210 6540
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6361 136
Size: 107 122 71 631 605 4689 1525
Fragments arranged by size:
4689 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7745 7745
Size: 7750
BlpI GC'TnA_GC
Cuts at: 3092 3622 3941 4703 5167 3092
Size: 530 319 762 464 5675
Fragments arranged by size:
5675 762 530 464 319
Bme1580I G_kGCm'C
Cuts at: 1790 2343 2363 2680 3184 3706 3958 4051 4090
Size: 553 20 317 504 522 252 93 39
Cuts at: 4090 5811 6967 1790
Size: 1721 1156 2573
Fragments arranged by size:
2573 1721 1156 553 522 504 317 252
93 39 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2873 3323 5481
Size: 416 53 83 399 24 1456 450 2158
Cuts at: 5481 6439 442
Size: 958 1753
Fragments arranged by size:
2158 1753 1456 958 450 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2106 1056
Size: 1050 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3510 4246 4654 3510
Size: 736 408 6606
Fragments arranged by size:
6606 736 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2466 2910 3408 3513 4820 6410 1239
Size: 1227 444 498 105 1307 1590 2579
Fragments arranged by size:
2579 1590 1307 1227 498 444 105
Bpu10I CC'TnA_GC
Cuts at: 3847 3895 4218 4569 3847
Size: 48 323 351 7028
Fragments arranged by size:
7028 351 323 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3059 4624 4871 4892 5875 6740 7038 3059
Size: 1565 247 21 983 865 298 3771
Fragments arranged by size:
3771 1565 983 865 298 247 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6413 882
Size: 617 4914 2219
Fragments arranged by size:
4914 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5047 5541 493
Size: 711 3843 494 2702
Fragments arranged by size:
3843 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7744 7744
Size: 7750
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5557 5939 6574 275
Size: 53 83 186 936 4024 382 635 1451
Fragments arranged by size:
4024 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4152 4294 4627 6183 6581 6855 7002 7413 4152
Size: 142 333 1556 398 274 147 411 4489
Fragments arranged by size:
4489 1556 411 398 333 274 147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 1178
Size: 30 165 30 782 30 6713
Fragments arranged by size:
6713 782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2245 2383 2388 2393 2496 2499 2785
Size: 937 122 138 5 5 103 3 286
Cuts at: 2785 2788 2791 2794 3422 3425 4224 4316 4328
Size: 3 3 3 628 3 799 92 12
Cuts at: 4328 4331 4340 4463 4466 4712 4817 1186
Size: 3 9 123 3 246 105 4119
Fragments arranged by size:
4119 937 799 628 286 246 138 123
122 105 103 92 12 9 5 5
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2078 2423 2461 2932 3699 4269 4281 4484 6953
Size: 345 38 471 767 570 12 203 2469
Cuts at: 6953 7449 2078
Size: 496 2379
Fragments arranged by size:
2469 2379 767 570 496 471 345 203
38 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2481 3332 3531 3867 4176 1163
Size: 573 745 851 199 336 309 4737
Fragments arranged by size:
4737 851 745 573 336 309 199
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5200 5961 6989 1540
Size: 275 186 3199 761 1028 2301
Fragments arranged by size:
3199 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3342 3561 4051 5811 5896 6967 3342
Size: 219 490 1760 85 1071 4125
Fragments arranged by size:
4125 1760 1071 490 219 85
BsmI GAATG_Cn'
Cuts at: 2981 5261 5354 2981
Size: 2280 93 5377
Fragments arranged by size:
5377 2280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 2363 2680 3063 3184 3342 3561
Size: 399 553 20 317 383 121 158 219
Cuts at: 3561 3623 3706 3765 3865 3958 4051 4090 5007
Size: 62 83 59 100 93 93 39 917
Cuts at: 5007 5069 5811 5896 6967 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 917 742 553 399 383 317
219 158 121 100 93 93 85 83
62 62 59 39 20
BspEI T'CCGG_A
Cuts at: 4152 7413 4152
Size: 3261 4489
BspHI T'CATG_A
Cuts at: 3561 5639 7369 3561
Size: 2078 1730 3942
Fragments arranged by size:
3942 2078 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2629 5105 844
Size: 1785 2476 3489
Fragments arranged by size:
3489 2476 1785
BsrBI CCG'CTC
Cuts at: 1394 1823 4837 4876 5637 1394
Size: 429 3014 39 761 3507
Fragments arranged by size:
3507 3014 761 429 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 3238 4725 6245 6419 65
Size: 1183 1990 1487 1520 174 1396
Fragments arranged by size:
1990 1520 1487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3285 3822 6394 6581 1700
Size: 110 1475 537 2572 187 2869
Fragments arranged by size:
2869 2572 1475 537 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7750
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7750
BssSI C'ACGA_G
Cuts at: 5810 6822 5810
Size: 1012 6738
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3476 794
Size: 2682 5068
BstBI TT'CG_AA
Cuts at: 2007 6554 2007
Size: 4547 3203
BstEII G'GTnAC_C
Cuts at: 1184 3841 1184
Size: 2657 5093
BstYI r'GATC_y
Cuts at: 1972 2347 2601 3189 4173 5080 5447 5832 5849
Size: 375 254 588 984 907 367 385 17
Cuts at: 5849 7301 7387 7399 7745 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 984 907 588 385 375 367
346 254 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2271 2289 2659 3856 2271
Size: 18 370 1197 6165
Fragments arranged by size:
6165 1197 370 18
BtgI C'CryG_G
Cuts at: 513 1065 2343 2994 3259 3522 4936 513
Size: 552 1278 651 265 263 1414 3327
Fragments arranged by size:
3327 1414 1278 651 552 265 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7011
Fragments arranged by size:
7011 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 3045 3178 3370 3576 3704 4597 5266 6060
Size: 497 133 192 206 128 893 669 794
Cuts at: 6060 6080 2548
Size: 20 4218
Fragments arranged by size:
4218 893 794 669 497 206 192 133
128 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7750
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3220 3255 3994 4029 529
Size: 35 2656 35 739 35 4250
Fragments arranged by size:
4250 2656 739 35 35 35
DraI TTT'AAA
Cuts at: 5054 5054
Size: 7750
DraIII CAC_nnn'GTG
Cuts at: 3476 3476
Size: 7750
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6757 809
Size: 507 5441 1802
Fragments arranged by size:
5441 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 2545 3973
Size: 54 1425 50 275 18 618 97 1428
Cuts at: 3973 4771 5197 6086 7715 8
Size: 798 426 889 1629 43
Fragments arranged by size:
1629 1428 1425 889 798 618 426 275
97 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5197 1537
Size: 275 3385 4090
Fragments arranged by size:
4090 3385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2251 2568 2725 2750 2812 2818 3907 4293
Size: 898 317 157 25 62 6 1089 386
Cuts at: 4293 4317 4335 4425 4608 4844 5680 1353
Size: 24 18 90 183 236 836 3423
Fragments arranged by size:
3423 1089 898 836 386 317 236 183
157 90 62 25 24 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2514 6337 6711 6857 387
Size: 1064 138 925 3823 374 146 1280
Fragments arranged by size:
3823 1280 1064 925 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2466 2731 2799 2910 3086 3408
Size: 393 738 96 265 68 111 176 322
Cuts at: 3408 3513 4221 4820 5813 6410 7197 7465 1239
Size: 105 708 599 993 597 787 268 1524
Fragments arranged by size:
1524 993 787 738 708 599 597 393
322 268 265 176 111 105 96 68
EcoNI CCTnn'n_nnAGG
Cuts at: 2706 4712 2706
Size: 2006 5744
EcoO109I rG'GnC_Cy
Cuts at: 2075 2331 2359 2437 3113 3219 3771 3955 3993
Size: 256 28 78 676 106 552 184 38
Cuts at: 3993 2075
Size: 5832
Fragments arranged by size:
5832 676 552 256 184 106 78 38
28
EcoRI G'AATT_C
Cuts at: 2165 4093 5059 5437 2165
Size: 1928 966 378 4478
Fragments arranged by size:
4478 1928 966 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 7750
FspI TGC'GCA
Cuts at: 2143 6256 2143
Size: 4113 3637
HaeII r_GCGC'y
Cuts at: 1678 6897 1678
Size: 5219 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 4292 4324 6391
Size: 32 135 32 743 32 1945 32 2067
Cuts at: 6391 6423 6465 6497 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2067 1945 743 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5094 5340 669
Size: 988 281 3156 246 3079
Fragments arranged by size:
3156 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7750
HpaI GTT'AAC
Cuts at: 5340 5340
Size: 7750
KpnI G_GTAC'C
Cuts at: 5075 5075
Size: 7750
MfeI C'AATT_G
Cuts at: 5349 5349
Size: 7750
MluI A'CGCG_T
Cuts at: 7626 7626
Size: 7750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6864 7048 1305
Size: 5559 184 2007
Fragments arranged by size:
5559 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2450 2547 3975 4773 10
Size: 54 1425 343 618 97 1428 798 2987
Fragments arranged by size:
2987 1428 1425 798 618 343 97 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3210 3984 4682 5708 6067 6226 518
Size: 648 2044 774 698 1026 359 159 2042
Fragments arranged by size:
2044 2042 1026 774 698 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2420 2596 2959 3851 4079 4484 5166
Size: 435 379 176 363 892 228 405 682
Cuts at: 5166 5843 6991 7236 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 892 682 677 435 405 379
363 245 228 176
NcoI C'CATG_G
Cuts at: 513 1065 4936 513
Size: 552 3871 3327
Fragments arranged by size:
3871 3327 552
NdeI CA'TA_TG
Cuts at: 387 4896 387
Size: 4509 3241
NheI G'CTAG_C
Cuts at: 1052 2102 1052
Size: 1050 6700
NotI GC'GGCC_GC
Cuts at: 5197 5197
Size: 7750
NspI r_CATG'y
Cuts at: 1132 1229 2190 3311 3667 4151 5108 6653 1132
Size: 97 961 1121 356 484 957 1545 2229
Fragments arranged by size:
2229 1545 1121 961 957 484 356 97
PasI CC'CwG_GG
Cuts at: 1348 2531 3056 3608 1348
Size: 1183 525 552 5490
Fragments arranged by size:
5490 1183 552 525
PciI A'CATG_T
Cuts at: 3307 6649 3307
Size: 3342 4408
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2327 2671 2863 3077 1108
Size: 1219 344 192 214 5781
Fragments arranged by size:
5781 1219 344 214 192
PfoI T'CCnGG_A
Cuts at: 3529 3529
Size: 7750
PmeI GTTT'AAAC
Cuts at: 5054 5054
Size: 7750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 5819 5851 7332 7364 2011
Size: 32 3776 32 1481 32 2397
Fragments arranged by size:
3776 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 2331 3113 2075
Size: 256 782 6712
Fragments arranged by size:
6712 782 256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7750
PsiI TTA'TAA
Cuts at: 5320 5320
Size: 7750
PspOMI G'GGCC_C
Cuts at: 2359 3954 2359
Size: 1595 6155
PstI C_TGCA'G
Cuts at: 830 2592 2636 3189 3801 4143 4713 4859 5102
Size: 1762 44 553 612 342 570 146 243
Cuts at: 5102 830
Size: 3478
Fragments arranged by size:
3478 1762 612 570 553 342 243 146
44
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7750
PvuII CAG'CTG
Cuts at: 2959 3851 4079 4484 2959
Size: 892 228 405 6225
Fragments arranged by size:
6225 892 405 228
SalI G'TCGA_C
Cuts at: 1936 5092 1936
Size: 3156 4594
SanDI GG'GwC_CC
Cuts at: 3113 3113
Size: 7750
SapI GCTCTTCn'nnn_
Cuts at: 2568 4844 2568
Size: 2276 5474
SbfI CC_TGCA'GG
Cuts at: 4713 5102 4713
Size: 389 7361
ScaI AGT'ACT
Cuts at: 5998 7710 5998
Size: 1712 6038
SexAI A'CCwGG_T
Cuts at: 4914 4914
Size: 7750
SfcI C'TryA_G
Cuts at: 826 1045 2588 2632 3185 3797 4139 4242 4709
Size: 219 1543 44 553 612 342 103 467
Cuts at: 4709 4855 5098 6233 6914 7105 826
Size: 146 243 1135 681 191 1471
Fragments arranged by size:
1543 1471 1135 681 612 553 467 342
243 219 191 146 103 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7750
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7750
SmaI CCC'GGG
Cuts at: 1872 4085 5077 1872
Size: 2213 992 4545
Fragments arranged by size:
4545 2213 992
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3038 4639 4871 4886 5854 6755
Size: 197 926 1095 1601 232 15 968 901
Cuts at: 6755 7017 820
Size: 262 1553
Fragments arranged by size:
1601 1553 1095 968 926 901 262 232
197 15
SnaBI TAC'GTA
Cuts at: 493 5047 5541 493
Size: 4554 494 2702
Fragments arranged by size:
4554 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7750
SphI G_CATG'C
Cuts at: 1132 3667 5108 1132
Size: 2535 1441 3774
Fragments arranged by size:
3774 2535 1441
SrfI GCCC'GGGC
Cuts at: 4085 4085
Size: 7750
SspI AAT'ATT
Cuts at: 5 52 4951 5674 5
Size: 47 4899 723 2081
Fragments arranged by size:
4899 2081 723 47
StuI AGG'CCT
Cuts at: 1517 2840 3036 4757 4778 1517
Size: 1323 196 1721 21 4489
Fragments arranged by size:
4489 1721 1323 196 21
StyI C'CwwG_G
Cuts at: 513 1065 3117 3765 4689 4824 4936 5189 513
Size: 552 2052 648 924 135 112 253 3074
Fragments arranged by size:
3074 2052 924 648 552 253 135 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2663 2874 3342 5777 5794 5947 6132
Size: 1255 1083 211 468 2435 17 153 185
Cuts at: 6132 6577 325
Size: 445 1498
Fragments arranged by size:
2435 1498 1255 1083 468 445 211 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5996 7708 96
Size: 274 80 33 51 1443 4019 1712 138
Fragments arranged by size:
4019 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3710 3742 7710 7742 3185
Size: 32 493 32 3968 32 3193
Fragments arranged by size:
3968 3193 493 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3350 1316
Size: 2034 5716
XbaI T'CTAG_A
Cuts at: 5086 5086
Size: 7750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 2779 3001 3634 4687 2458
Size: 321 222 633 1053 5521
Fragments arranged by size:
5521 1053 633 321 222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7750
XmaI C'CCGG_G
Cuts at: 1870 4083 5075 1870
Size: 2213 992 4545
Fragments arranged by size:
4545 2213 992
XmnI GAAnn'nnTTC
Cuts at: 4118 5879 4118
Size: 1761 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5558 276
Size: 53 83 186 936 4024 2468
Fragments arranged by size:
4024 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SfiI SfoI SwaI