Restriction map for
pFN21ASDA0359
(Circular) (Six-base) MAPSORT of: pFN21ASDA0359.seq Check: 4999 from: 1 to: 6970
pFN21ASDA0359 6970 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2131 2131
Size: 6970
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4780 278
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4313 1937
Size: 2376 4594
Acc65I G'GTAC_C
Cuts at: 4291 4291
Size: 6970
AclI AA'CG_TT
Cuts at: 1563 5097 5470 1563
Size: 3534 373 3063
Fragments arranged by size:
3534 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 3650 5033 6417 6685 1632
Size: 1739 279 1383 1384 268 1917
Fragments arranged by size:
1917 1739 1384 1383 279 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6773
AflIII A'CryG_T
Cuts at: 1203 5869 6846 1203
Size: 4666 977 1327
Fragments arranged by size:
4666 1327 977
AgeI A'CCGG_T
Cuts at: 5801 5801
Size: 6970
AhdI GACnn_n'nnGTC
Cuts at: 5699 5699
Size: 6970
AleI CACnn'nnGTG
Cuts at: 1166 2540 2730 1166
Size: 1374 190 5406
Fragments arranged by size:
5406 1374 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6970
AlwNI CAG_nnn'CTG
Cuts at: 4713 6285 4713
Size: 1572 5398
ApaLI G'TGCA_C
Cuts at: 5027 6183 5027
Size: 1156 5814
ApoI r'AATT_y
Cuts at: 1493 3092 4279 4505 4657 6766 1493
Size: 1599 1187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1599 1187 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5524 160
Size: 872 4492 1606
Fragments arranged by size:
4492 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
AvaI C'yCGr_G
Cuts at: 1870 1943 4245 4295 1870
Size: 73 2302 50 4545
Fragments arranged by size:
4545 2302 73 50
BamHI G'GATC_C
Cuts at: 4193 4300 4667 4193
Size: 107 367 6496
Fragments arranged by size:
6496 367 107
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3413 4291 5746 618
Size: 325 217 627 1626 878 1455 1842
Fragments arranged by size:
1842 1626 1455 878 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2520 3750 4289 1391
Size: 1129 1230 539 4072
Fragments arranged by size:
4072 1230 1129 539
BbeI G_GCGC'C
Cuts at: 3417 3417
Size: 6970
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 928
Size: 971 654 5345
Fragments arranged by size:
5345 971 654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3515 3549 5161 5195 1903
Size: 34 1578 34 1612 34 3678
Fragments arranged by size:
3678 1612 1578 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4862 6072 4862
Size: 1210 5760
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6970
BfrBI ATG'CAT
Cuts at: 4127 4127
Size: 6970
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5581 136
Size: 107 122 71 631 605 3909 1525
Fragments arranged by size:
3909 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2460 2755 6965 2460
Size: 295 4210 2465
Fragments arranged by size:
4210 2465 295
BlpI GC'TnA_GC
Cuts at: 4387 4387
Size: 6970
Bme1580I G_kGCm'C
Cuts at: 1790 5031 6187 1790
Size: 3241 1156 2573
Fragments arranged by size:
3241 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6970
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 4701 5659
Size: 416 53 83 399 24 908 2376 958
Cuts at: 5659 442
Size: 1753
Fragments arranged by size:
2376 1753 958 908 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2674 2504
Size: 170 6800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3305 3518 3902 5630 1239
Size: 2066 213 384 1728 2579
Fragments arranged by size:
2579 2066 1728 384 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 3101 3752 5095 5960 6258 2534
Size: 567 651 1343 865 298 3246
Fragments arranged by size:
3246 1343 865 651 567 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3572 3925 5633 882
Size: 617 2073 353 1708 2219
Fragments arranged by size:
2219 2073 1708 617 353
BsaAI yAC'GTr
Cuts at: 493 1204 4267 4761 493
Size: 711 3063 494 2702
Fragments arranged by size:
3063 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6964 6964
Size: 6970
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3414 4777 5159 5794
Size: 53 83 186 936 1881 1363 382 635
Cuts at: 5794 275
Size: 1451
Fragments arranged by size:
1881 1451 1363 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5403 5801 6075 6222 6633 5403
Size: 398 274 147 411 5740
Fragments arranged by size:
5740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2532 2562 3163 3193 3425
Size: 30 165 30 1129 30 601 30 232
Cuts at: 3425 3455 1178
Size: 30 4693
Fragments arranged by size:
4693 1129 601 232 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2385 2486 2912 3221 3224 3227 3230 3248
Size: 1199 101 426 309 3 3 3 18
Cuts at: 3248 3347 3590 4189 4192 1186
Size: 99 243 599 3 3964
Fragments arranged by size:
3964 1199 599 426 309 243 101 99
18 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2134 3123 3409 4025 6173 6669 2134
Size: 989 286 616 2148 496 2435
Fragments arranged by size:
2435 2148 989 616 496 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2716 1163
Size: 573 980 5417
Fragments arranged by size:
5417 980 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4420 5181 6209 1540
Size: 275 186 2419 761 1028 2301
Fragments arranged by size:
2419 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 2655 3750 5031 5116 6187 2520
Size: 135 1095 1281 85 1071 3303
Fragments arranged by size:
3303 1281 1095 1071 135 85
BsmI GAATG_Cn'
Cuts at: 2224 4481 4574 2224
Size: 2257 93 4620
Fragments arranged by size:
4620 2257 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2240 2240
Size: 6970
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 2655 3750 4289 5031 5116 6187
Size: 399 730 135 1095 539 742 85 1071
Cuts at: 6187 1391
Size: 2174
Fragments arranged by size:
2174 1095 1071 742 730 539 399 135
85
BspEI T'CCGG_A
Cuts at: 6633 6633
Size: 6970
BspHI T'CATG_A
Cuts at: 4859 6589 4859
Size: 1730 5240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 3387 4325 844
Size: 1287 1256 938 3489
Fragments arranged by size:
3489 1287 1256 938
BsrBI CCG'CTC
Cuts at: 1394 1823 4857 1394
Size: 429 3034 3507
Fragments arranged by size:
3507 3034 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2950 3608 4059 5465 5639 65
Size: 1183 1702 658 451 1406 174 1396
Fragments arranged by size:
1702 1406 1396 1183 658 451 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2786 5614 5801 1700
Size: 110 976 2828 187 2869
Fragments arranged by size:
2869 2828 976 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6970
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6970
BssSI C'ACGA_G
Cuts at: 5030 6042 5030
Size: 1012 5958
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4088 794
Size: 3294 3676
BstBI TT'CG_AA
Cuts at: 2007 5774 2007
Size: 3767 3203
BstEII G'GTnAC_C
Cuts at: 1184 2354 1184
Size: 1170 5800
BstYI r'GATC_y
Cuts at: 1972 2460 2475 2755 3684 4193 4300 4667 5052
Size: 488 15 280 929 509 107 367 385
Cuts at: 5052 5069 6521 6607 6619 6965 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 929 509 488 385 367 346
280 107 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 4210 4210
Size: 6970
BtgI C'CryG_G
Cuts at: 513 1065 2337 2350 3429 3918 513
Size: 552 1272 13 1079 489 3565
Fragments arranged by size:
3565 1272 1079 552 489 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6231
Fragments arranged by size:
6231 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3678 4143 4486 5280 5300 3193
Size: 485 465 343 794 20 4863
Fragments arranged by size:
4863 794 485 465 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6935
DraI TTT'AAA
Cuts at: 4274 4274
Size: 6970
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5977 809
Size: 507 4661 1802
Fragments arranged by size:
4661 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2964 3905 4417
Size: 54 1425 50 275 18 1134 941 512
Cuts at: 4417 5306 6935 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1134 941 889 512 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4417 1537
Size: 275 2605 4090
Fragments arranged by size:
4090 2605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2991 3096 3149 3198 3219 3659 3837 4210
Size: 1638 105 53 49 21 440 178 373
Cuts at: 4210 4900 1353
Size: 690 3423
Fragments arranged by size:
3423 1638 690 440 373 178 105 53
49 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5557 5931 6077 387
Size: 1064 138 3968 374 146 1280
Fragments arranged by size:
3968 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2518 3748 2518
Size: 1230 5740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3305 3371 3518 3650 3902 5033 5630
Size: 393 1673 66 147 132 252 1131 597
Cuts at: 5630 6417 6685 1239
Size: 787 268 1524
Fragments arranged by size:
1673 1524 1131 787 597 393 268 252
147 132 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2535 2535
Size: 6970
EcoO109I rG'GnC_Cy
Cuts at: 2975 3067 3376 2975
Size: 92 309 6569
Fragments arranged by size:
6569 309 92
EcoRI G'AATT_C
Cuts at: 3092 4279 4657 3092
Size: 1187 378 5405
Fragments arranged by size:
5405 1187 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2951 2951
Size: 6970
FspI TGC'GCA
Cuts at: 5476 5476
Size: 6970
HaeII r_GCGC'y
Cuts at: 1678 3417 3721 6117 1678
Size: 1739 304 2396 2531
Fragments arranged by size:
2531 2396 1739 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 3550 3582 3955
Size: 32 135 32 902 32 1044 32 373
Cuts at: 3955 3987 4115 4147 5611 5643 5685 5717 1373
Size: 32 128 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 1044 902 373 135 128 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 4314 4560 669
Size: 988 281 328 2048 246 3079
Fragments arranged by size:
3079 2048 988 328 281 246
HindIII A'AGCT_T
Cuts at: 748 2909 3662 748
Size: 2161 753 4056
Fragments arranged by size:
4056 2161 753
HpaI GTT'AAC
Cuts at: 4560 4560
Size: 6970
KasI G'GCGC_C
Cuts at: 3413 3413
Size: 6970
KpnI G_GTAC'C
Cuts at: 4295 4295
Size: 6970
MfeI C'AATT_G
Cuts at: 4569 4569
Size: 6970
MluI A'CGCG_T
Cuts at: 6846 6846
Size: 6970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 3430 6084 6268 1305
Size: 973 1152 2654 184 2007
Fragments arranged by size:
2654 2007 1152 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2966 10
Size: 54 1425 343 1134 4014
Fragments arranged by size:
4014 1425 1134 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 2730 3477 4928 5287 5446 518
Size: 648 1374 190 747 1451 359 159 2042
Fragments arranged by size:
2042 1451 1374 747 648 359 190 159
MspA1I CmG'CkG
Cuts at: 1606 3127 4386 5063 6211 6456 1606
Size: 1521 1259 677 1148 245 2120
Fragments arranged by size:
2120 1521 1259 1148 677 245
NarI GG'CG_CC
Cuts at: 3414 3414
Size: 6970
NcoI C'CATG_G
Cuts at: 513 1065 2337 3429 513
Size: 552 1272 1092 4054
Fragments arranged by size:
4054 1272 1092 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6970
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6970
NotI GC'GGCC_GC
Cuts at: 4417 4417
Size: 6970
NsiI A_TGCA'T
Cuts at: 4129 4129
Size: 6970
NspI r_CATG'y
Cuts at: 1132 1229 2600 4024 4328 5873 1132
Size: 97 1371 1424 304 1545 2229
Fragments arranged by size:
2229 1545 1424 1371 304 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6970
PciI A'CATG_T
Cuts at: 5869 5869
Size: 6970
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6970
PfoI T'CCnGG_A
Cuts at: 3096 3495 3096
Size: 399 6571
PmeI GTTT'AAAC
Cuts at: 4274 4274
Size: 6970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5039 5071 6552 6584 5039
Size: 32 1481 32 5425
Fragments arranged by size:
5425 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3067 3376 3067
Size: 309 6661
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6970
PsiI TTA'TAA
Cuts at: 4540 4540
Size: 6970
PstI C_TGCA'G
Cuts at: 830 4101 4322 830
Size: 3271 221 3478
Fragments arranged by size:
3478 3271 221
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6970
PvuII CAG'CTG
Cuts at: 3127 3127
Size: 6970
SacI G_AGCT'C
Cuts at: 2520 3750 2520
Size: 1230 5740
SalI G'TCGA_C
Cuts at: 1936 4312 1936
Size: 2376 4594
SapI GCTCTTCn'nnn_
Cuts at: 3659 3659
Size: 6970
SbfI CC_TGCA'GG
Cuts at: 4322 4322
Size: 6970
ScaI AGT'ACT
Cuts at: 5218 6930 5218
Size: 1712 5258
SexAI A'CCwGG_T
Cuts at: 2436 2436
Size: 6970
SfcI C'TryA_G
Cuts at: 826 1045 4097 4318 5453 6134 6325 826
Size: 219 3052 221 1135 681 191 1471
Fragments arranged by size:
3052 1471 1135 681 221 219 191
SfoI GGC'GCC
Cuts at: 3415 3415
Size: 6970
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6970
SmaI CCC'GGG
Cuts at: 1872 4297 1872
Size: 2425 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3116 3767 5074 5975 6237
Size: 197 926 570 603 651 1307 901 262
Cuts at: 6237 820
Size: 1553
Fragments arranged by size:
1553 1307 926 901 651 603 570 262
197
SnaBI TAC'GTA
Cuts at: 493 4267 4761 493
Size: 3774 494 2702
Fragments arranged by size:
3774 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6970
SphI G_CATG'C
Cuts at: 1132 4328 1132
Size: 3196 3774
SspI AAT'ATT
Cuts at: 5 52 4894 5
Size: 47 4842 2081
Fragments arranged by size:
4842 2081 47
StuI AGG'CCT
Cuts at: 1517 2530 4171 1517
Size: 1013 1641 4316
Fragments arranged by size:
4316 1641 1013
StyI C'CwwG_G
Cuts at: 513 1065 2337 2935 3053 3072 3429 3582 4068
Size: 552 1272 598 118 19 357 153 486
Cuts at: 4068 4161 4409 513
Size: 93 248 3074
Fragments arranged by size:
3074 1272 598 552 486 357 248 153
118 93 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 4997 5014 5167 5352 5797 325
Size: 1255 840 2577 17 153 185 445 1498
Fragments arranged by size:
2577 1498 1255 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5216 6928 96
Size: 274 80 33 51 1443 3239 1712 138
Fragments arranged by size:
3239 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2532 2564 2875 2907 3163 3195 6930 6962 2532
Size: 32 311 32 256 32 3735 32 2540
Fragments arranged by size:
3735 2540 311 256 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2270 1316
Size: 954 6016
XbaI T'CTAG_A
Cuts at: 4306 4306
Size: 6970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2933 2956 2933
Size: 23 6947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6970
XmaI C'CCGG_G
Cuts at: 1870 4295 1870
Size: 2425 4545
XmnI GAAnn'nnTTC
Cuts at: 5099 5099
Size: 6970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4778 276
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SrfI SwaI