Restriction map for
pFN21ASDA0355
(Circular) (Six-base) MAPSORT of: pFN21ASDA0355.seq Check: 9763 from: 1 to: 7939
pFN21ASDA0355 7939 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5749 278
Size: 53 83 186 936 4213 2468
Fragments arranged by size:
4213 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5282 1937
Size: 3345 4594
Acc65I G'GTAC_C
Cuts at: 5260 5260
Size: 7939
AclI AA'CG_TT
Cuts at: 1563 6066 6439 1563
Size: 4503 373 3063
Fragments arranged by size:
4503 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2308 2706 3095 3222 3591 6002 7386 7654
Size: 676 398 389 127 369 2411 1384 268
Cuts at: 7654 1632
Size: 1917
Fragments arranged by size:
2411 1917 1384 676 398 389 369 268
127
AflII C'TTAA_G
Cuts at: 820 1017 3119 820
Size: 197 2102 5640
Fragments arranged by size:
5640 2102 197
AflIII A'CryG_T
Cuts at: 1203 6838 7815 1203
Size: 5635 977 1327
Fragments arranged by size:
5635 1327 977
AgeI A'CCGG_T
Cuts at: 6770 6770
Size: 7939
AhdI GACnn_n'nnGTC
Cuts at: 3450 6668 3450
Size: 3218 4721
AleI CACnn'nnGTG
Cuts at: 1166 3476 4857 4907 1166
Size: 2310 1381 50 4198
Fragments arranged by size:
4198 2310 1381 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2224 4056 1184
Size: 1040 1832 5067
Fragments arranged by size:
5067 1832 1040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 4781 4813 2766
Size: 32 1983 32 5892
Fragments arranged by size:
5892 1983 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3265 4817 5065 5682 7254 3265
Size: 1552 248 617 1572 3950
Fragments arranged by size:
3950 1572 1552 617 248
ApaLI G'TGCA_C
Cuts at: 5996 7152 5996
Size: 1156 6783
ApoI r'AATT_y
Cuts at: 1493 2476 2495 3847 5248 5474 5626 7735 1493
Size: 983 19 1352 1401 226 152 2109 1697
Fragments arranged by size:
2109 1697 1401 1352 983 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 6493 160
Size: 872 5461 1606
Fragments arranged by size:
5461 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7939
AvaI C'yCGr_G
Cuts at: 1870 1943 3982 4272 4641 4691 4759 5264 1870
Size: 73 2039 290 369 50 68 505 4545
Fragments arranged by size:
4545 2039 505 369 290 73 68 50
BamHI G'GATC_C
Cuts at: 5269 5636 5269
Size: 367 7572
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3239 3384 4126 4174 4276
Size: 325 217 627 1452 145 742 48 102
Cuts at: 4276 5260 6715 618
Size: 984 1455 1842
Fragments arranged by size:
1842 1455 1452 984 742 627 325 217
145 102 48
BanII G_rGCy'C
Cuts at: 1391 3039 4016 4550 5258 1391
Size: 1648 977 534 708 4072
Fragments arranged by size:
4072 1648 977 708 534
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2281 3402 3449 3629 928
Size: 971 382 1121 47 180 5238
Fragments arranged by size:
5238 1121 971 382 180 47
BbvCI CC'TCA_GC
Cuts at: 2616 2724 4179 4777 2616
Size: 108 1455 598 5778
Fragments arranged by size:
5778 1455 598 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3225 3259 5084 5118 6130 6164 1903
Size: 34 1288 34 1825 34 1012 34 3678
Fragments arranged by size:
3678 1825 1288 1012 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5831 7041 5831
Size: 1210 6729
BclI T'GATC_A
Cuts at: 1548 3949 1548
Size: 2401 5538
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4632 6550 136
Size: 107 122 71 631 605 2960 1918 1525
Fragments arranged by size:
2960 1918 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2317 3552 7934 2317
Size: 1235 4382 2322
Fragments arranged by size:
4382 2322 1235
BlpI GC'TnA_GC
Cuts at: 5356 5356
Size: 7939
Bme1580I G_kGCm'C
Cuts at: 1790 2041 4113 4129 4144 4279 6000 7156 1790
Size: 251 2072 16 15 135 1721 1156 2573
Fragments arranged by size:
2573 2072 1721 1156 251 135 16 15
BmgBI CAC'GTC
Cuts at: 1318 3463 1318
Size: 2145 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5069 5221 5670
Size: 416 53 83 399 24 3652 152 449
Cuts at: 5670 6628 442
Size: 958 1753
Fragments arranged by size:
3652 1753 958 449 416 399 152 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4792 4792
Size: 7939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 3517 3661 4220 6599 1239
Size: 1250 1028 144 559 2379 2579
Fragments arranged by size:
2579 2379 1250 1028 559 144
Bpu10I CC'TnA_GC
Cuts at: 2127 2616 2724 2820 2893 4179 4740 4777 2127
Size: 489 108 96 73 1286 561 37 5289
Fragments arranged by size:
5289 1286 561 489 108 96 73 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4194 6064 6929 7227 4194
Size: 1870 865 298 4906
Fragments arranged by size:
4906 1870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6602 882
Size: 617 5103 2219
Fragments arranged by size:
5103 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5236 5730 493
Size: 711 4032 494 2702
Fragments arranged by size:
4032 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7933 7933
Size: 7939
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3081 5746 6128 6763
Size: 53 83 186 936 1548 2665 382 635
Cuts at: 6763 275
Size: 1451
Fragments arranged by size:
2665 1548 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6372 6770 7044 7191 7602 6372
Size: 398 274 147 411 6709
Fragments arranged by size:
6709 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4218 4248 4881 4911 1178
Size: 30 165 30 2815 30 633 30 4206
Fragments arranged by size:
4206 2815 633 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4332 4889 1186
Size: 3146 557 4236
Fragments arranged by size:
4236 3146 557
BseYI C'CCAG_C
Cuts at: 2131 2465 2988 4136 4161 4266 4305 4513 4614
Size: 334 523 1148 25 105 39 208 101
Cuts at: 4614 4985 4997 5003 5136 7142 7638 2131
Size: 371 12 6 133 2006 496 2432
Fragments arranged by size:
2432 2006 1148 523 496 371 334 208
133 105 101 39 25 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 2402 2829 3074 3278 4703 5144
Size: 573 384 282 427 245 204 1425 441
Cuts at: 5144 1163
Size: 3958
Fragments arranged by size:
3958 1425 573 441 427 384 282 245
204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3102 4624 5389 6150 7178 1540
Size: 275 186 1101 1522 765 761 1028 2301
Fragments arranged by size:
2301 1522 1101 1028 765 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2143 2152 2826 3039 3468 4741 6000 6085 7156
Size: 9 674 213 429 1273 1259 85 1071
Cuts at: 7156 2143
Size: 2926
Fragments arranged by size:
2926 1273 1259 1071 674 429 213 85
9
BsmI GAATG_Cn'
Cuts at: 4515 5450 5543 4515
Size: 935 93 6911
Fragments arranged by size:
6911 935 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3088 3088
Size: 7939
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2143 2152 2826 3039 3468 4016
Size: 399 251 102 9 674 213 429 548
Cuts at: 4016 4113 4129 4144 4279 4550 4741 5258 6000
Size: 97 16 15 135 271 191 517 742
Cuts at: 6000 6085 7156 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 548 517 429 399
271 251 213 191 135 102 97 85
16 15 9
BspEI T'CCGG_A
Cuts at: 7602 7602
Size: 7939
BspHI T'CATG_A
Cuts at: 5828 7558 5828
Size: 1730 6209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3588 4322 5294 844
Size: 2744 734 972 3489
Fragments arranged by size:
3489 2744 972 734
BsrBI CCG'CTC
Cuts at: 1394 1823 5826 1394
Size: 429 4003 3507
Fragments arranged by size:
4003 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2919 6434 6608 65
Size: 1183 1671 3515 174 1396
Fragments arranged by size:
3515 1671 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2593 2921 4626 6583 6770 1700
Size: 110 783 328 1705 1957 187 2869
Fragments arranged by size:
2869 1957 1705 783 328 187 110
BsrGI T'GTAC_A
Cuts at: 96 4386 96
Size: 4290 3649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7939
BssSI C'ACGA_G
Cuts at: 5999 7011 5999
Size: 1012 6927
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 2147 3025 5065 794
Size: 1304 49 878 2040 3668
Fragments arranged by size:
3668 2040 1304 878 49
BstBI TT'CG_AA
Cuts at: 2007 6743 2007
Size: 4736 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7939
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 3381 4054 4244 4496 2272
Size: 1109 673 190 252 5715
Fragments arranged by size:
5715 1109 673 252 190
BstYI r'GATC_y
Cuts at: 1972 2317 3534 3552 5269 5636 6021 6038 7490
Size: 345 1217 18 1717 367 385 17 1452
Cuts at: 7490 7576 7588 7934 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1717 1452 1217 385 367 346 345
86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 4053 4121 4905 513
Size: 552 1098 1890 68 784 3547
Fragments arranged by size:
3547 1890 1098 784 552 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2542 4607 487
Size: 683 56 1316 2065 3819
Fragments arranged by size:
3819 2065 1316 683 56
BtsI GCAGTG_nn'
Cuts at: 2041 2172 2656 3035 4100 4548 4864 5107 5455
Size: 131 484 379 1065 448 316 243 348
Cuts at: 5455 6249 6269 2041
Size: 794 20 3711
Fragments arranged by size:
3711 1065 794 484 448 379 348 316
243 131 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7904
DraI TTT'AAA
Cuts at: 5243 5243
Size: 7939
DraIII CAC_nnn'GTG
Cuts at: 2147 3384 2147
Size: 1237 6702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6946 809
Size: 507 5630 1802
Fragments arranged by size:
5630 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2421 3099
Size: 54 1425 50 275 18 336 255 678
Cuts at: 3099 3271 3864 4628 4652 4946 5386 6275 7904
Size: 172 593 764 24 294 440 889 1629
Cuts at: 7904 8
Size: 43
Fragments arranged by size:
1629 1425 889 764 678 593 440 336
294 275 255 172 54 50 43 24
18
EagI C'GGCC_G
Cuts at: 1537 1812 3099 5386 1537
Size: 275 1287 2287 4090
Fragments arranged by size:
4090 2287 1287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 3741 5869 1353
Size: 974 1414 2128 3423
Fragments arranged by size:
3423 2128 1414 974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2996 3016 6526 6900 7046 387
Size: 1064 138 1407 20 3510 374 146 1280
Fragments arranged by size:
3510 1407 1280 1064 374 146 138 20
EcoICRI GAG'CTC
Cuts at: 3037 3037
Size: 7939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 2489 2706 3095 3222 3517 3591
Size: 393 676 181 217 389 127 295 74
Cuts at: 3591 3661 4220 6002 6599 7386 7654 1239
Size: 70 559 1782 597 787 268 1524
Fragments arranged by size:
1782 1524 787 676 597 559 393 389
295 268 217 181 127 74 70
EcoO109I rG'GnC_Cy
Cuts at: 4457 4814 4457
Size: 357 7582
EcoRI G'AATT_C
Cuts at: 2495 3847 5248 5626 2495
Size: 1352 1401 378 4808
Fragments arranged by size:
4808 1401 1352 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 7939
FspI TGC'GCA
Cuts at: 6445 6445
Size: 7939
HaeII r_GCGC'y
Cuts at: 1678 2071 7086 1678
Size: 393 5015 2531
Fragments arranged by size:
5015 2531 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2717 2749 3436 3468 3965
Size: 32 135 32 1145 32 687 32 497
Cuts at: 3965 3997 4757 4789 6580 6612 6654 6686 1373
Size: 32 760 32 1791 32 42 32 2626
Fragments arranged by size:
2626 1791 1145 760 687 497 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3295 5283 5529 669
Size: 988 281 1357 1988 246 3079
Fragments arranged by size:
3079 1988 1357 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2890 3516 3660 748
Size: 2142 626 144 5027
Fragments arranged by size:
5027 2142 626 144
HpaI GTT'AAC
Cuts at: 5529 5529
Size: 7939
KpnI G_GTAC'C
Cuts at: 5264 5264
Size: 7939
MfeI C'AATT_G
Cuts at: 5538 5538
Size: 7939
MluI A'CGCG_T
Cuts at: 7815 7815
Size: 7939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4306 7053 7237 1305
Size: 3001 2747 184 2007
Fragments arranged by size:
3001 2747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3273 3866 4948 10
Size: 54 1425 343 1441 593 1082 3001
Fragments arranged by size:
3001 1441 1425 1082 593 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 3379 3476 3909 4052 4857 4907
Size: 648 1350 863 97 433 143 805 50
Cuts at: 4907 5060 5897 6256 6415 518
Size: 153 837 359 159 2042
Fragments arranged by size:
2042 1350 863 837 805 648 433 359
159 153 143 97 50
MspA1I CmG'CkG
Cuts at: 1606 2198 2208 2646 3142 3262 3476 3495 3541
Size: 592 10 438 496 120 214 19 46
Cuts at: 3541 5108 5355 6032 7180 7425 1606
Size: 1567 247 677 1148 245 2120
Fragments arranged by size:
2120 1567 1148 677 592 496 438 247
245 214 120 46 19 10
NaeI GCC'GGC
Cuts at: 2923 4628 2923
Size: 1705 6234
NcoI C'CATG_G
Cuts at: 513 1065 4053 513
Size: 552 2988 4399
Fragments arranged by size:
4399 2988 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7939
NgoMIV G'CCGG_C
Cuts at: 2921 4626 2921
Size: 1705 6234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7939
NotI GC'GGCC_GC
Cuts at: 5386 5386
Size: 7939
NspI r_CATG'y
Cuts at: 1132 1229 2393 5297 6842 1132
Size: 97 1164 2904 1545 2229
Fragments arranged by size:
2904 2229 1545 1164 97
PasI CC'CwG_GG
Cuts at: 1348 2182 3448 5192 1348
Size: 834 1266 1744 4095
Fragments arranged by size:
4095 1744 1266 834
PciI A'CATG_T
Cuts at: 6838 6838
Size: 7939
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 4944 1108
Size: 1488 2348 4103
Fragments arranged by size:
4103 2348 1488
PfoI T'CCnGG_A
Cuts at: 2505 2505
Size: 7939
PmeI GTTT'AAAC
Cuts at: 5243 5243
Size: 7939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6008 6040 7521 7553 6008
Size: 32 1481 32 6394
Fragments arranged by size:
6394 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4457 4457
Size: 7939
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7939
PsiI TTA'TAA
Cuts at: 5509 5509
Size: 7939
PspXI vC'TCGA_Gb
Cuts at: 4759 4759
Size: 7939
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2333 2365 2333
Size: 32 7907
PstI C_TGCA'G
Cuts at: 830 2036 2203 2975 3423 3621 4543 5070 5102
Size: 1206 167 772 448 198 922 527 32
Cuts at: 5102 5291 830
Size: 189 3478
Fragments arranged by size:
3478 1206 922 772 527 448 198 189
167 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7939
PvuII CAG'CTG
Cuts at: 2208 3142 3262 3476 3541 2208
Size: 934 120 214 65 6606
Fragments arranged by size:
6606 934 214 120 65
SacI G_AGCT'C
Cuts at: 3039 3039
Size: 7939
SalI G'TCGA_C
Cuts at: 1936 5281 1936
Size: 3345 4594
SbfI CC_TGCA'GG
Cuts at: 5291 5291
Size: 7939
ScaI AGT'ACT
Cuts at: 2305 5231 6187 7899 2305
Size: 2926 956 1712 2345
Fragments arranged by size:
2926 2345 1712 956
SexAI A'CCwGG_T
Cuts at: 3213 3213
Size: 7939
SfcI C'TryA_G
Cuts at: 826 1045 2032 2199 2971 3419 3617 3822 4539
Size: 219 987 167 772 448 198 205 717
Cuts at: 4539 5066 5098 5287 6422 7103 7294 826
Size: 527 32 189 1135 681 191 1471
Fragments arranged by size:
1471 1135 987 772 717 681 527 448
219 205 198 191 189 167 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7939
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7939
SmaI CCC'GGG
Cuts at: 1872 4643 5266 1872
Size: 2771 623 4545
Fragments arranged by size:
4545 2771 623
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3119 4209 4759 6043 6944 7206
Size: 197 926 1176 1090 550 1284 901 262
Cuts at: 7206 820
Size: 1553
Fragments arranged by size:
1553 1284 1176 1090 926 901 550 262
197
SnaBI TAC'GTA
Cuts at: 493 5236 5730 493
Size: 4743 494 2702
Fragments arranged by size:
4743 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7939
SphI G_CATG'C
Cuts at: 1132 5297 1132
Size: 4165 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5863 5
Size: 47 3720 2091 2081
Fragments arranged by size:
3720 2091 2081 47
StuI AGG'CCT
Cuts at: 1517 5186 1517
Size: 3669 4270
StyI C'CwwG_G
Cuts at: 513 1065 4053 5172 5378 513
Size: 552 2988 1119 206 3074
Fragments arranged by size:
3074 2988 1119 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4966 5185 5966 5983 6136 6321 6766
Size: 1255 3386 219 781 17 153 185 445
Cuts at: 6766 325
Size: 1498
Fragments arranged by size:
3386 1498 1255 781 445 219 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2303 4386 5229
Size: 274 80 33 51 1443 326 2083 843
Cuts at: 5229 6185 7897 96
Size: 956 1712 138
Fragments arranged by size:
2083 1712 1443 956 843 326 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3273 3305 4881 4913 7899 7931 3273
Size: 32 1576 32 2986 32 3281
Fragments arranged by size:
3281 2986 1576 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7939
XbaI T'CTAG_A
Cuts at: 2735 3353 5275 2735
Size: 618 1922 5399
Fragments arranged by size:
5399 1922 618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2449 3859 2179
Size: 270 1410 6259
Fragments arranged by size:
6259 1410 270
XhoI C'TCGA_G
Cuts at: 1943 4759 1943
Size: 2816 5123
XmaI C'CCGG_G
Cuts at: 1870 4641 5264 1870
Size: 2771 623 4545
Fragments arranged by size:
4545 2771 623
XmnI GAAnn'nnTTC
Cuts at: 2288 2495 3626 6068 2288
Size: 207 1131 2442 4159
Fragments arranged by size:
4159 2442 1131 207
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5747 276
Size: 53 83 186 936 4213 2468
Fragments arranged by size:
4213 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI