Restriction map for
pFN21ASDA0353
(Circular) (Six-base) MAPSORT of: pFN21ASDA0353.seq Check: 223 from: 1 to: 9424
pFN21ASDA0353 9424 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2908 3455 4475 2908
Size: 547 1020 7857
Fragments arranged by size:
7857 1020 547
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7234 278
Size: 53 83 186 936 5698 2468
Fragments arranged by size:
5698 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6106 6630 6767 1937
Size: 4169 524 137 4594
Fragments arranged by size:
4594 4169 524 137
Acc65I G'GTAC_C
Cuts at: 5792 6745 5792
Size: 953 8471
AclI AA'CG_TT
Cuts at: 1563 7551 7924 1563
Size: 5988 373 3063
Fragments arranged by size:
5988 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3983 5046 5200 5235 5439 7487 8871 9139
Size: 2351 1063 154 35 204 2048 1384 268
Cuts at: 9139 1632
Size: 1917
Fragments arranged by size:
2351 2048 1917 1384 1063 268 204 154
35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9227
AflIII A'CryG_T
Cuts at: 1203 2097 8323 9300 1203
Size: 894 6226 977 1327
Fragments arranged by size:
6226 1327 977 894
AgeI A'CCGG_T
Cuts at: 3842 8255 3842
Size: 4413 5011
AhdI GACnn_n'nnGTC
Cuts at: 6637 8153 6637
Size: 1516 7908
AleI CACnn'nnGTG
Cuts at: 1166 4031 1166
Size: 2865 6559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3292 3324 3292
Size: 32 9392
AlwNI CAG_nnn'CTG
Cuts at: 2233 2314 4867 5162 5371 5636 7167 8739 2233
Size: 81 2553 295 209 265 1531 1572 2918
Fragments arranged by size:
2918 2553 1572 1531 295 265 209 81
ApaI G_GGCC'C
Cuts at: 2051 5315 5738 6387 2051
Size: 3264 423 649 5088
Fragments arranged by size:
5088 3264 649 423
ApaLI G'TGCA_C
Cuts at: 2570 6050 6074 7481 8637 2570
Size: 3480 24 1407 1156 3357
Fragments arranged by size:
3480 3357 1407 1156 24
ApoI r'AATT_y
Cuts at: 1493 3029 4766 4817 6733 6959 7111 9220 1493
Size: 1536 1737 51 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1737 1697 1536 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 7978 160
Size: 872 6946 1606
Fragments arranged by size:
6946 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9424
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2411 2747 3223 4538 4904 5232
Size: 73 108 360 336 476 1315 366 328
Cuts at: 5232 5290 6100 6749 1870
Size: 58 810 649 4545
Fragments arranged by size:
4545 1315 810 649 476 366 360 336
328 108 73 58
AvrII C'CTAG_G
Cuts at: 5590 5590
Size: 9424
BamHI G'GATC_C
Cuts at: 6754 7121 6754
Size: 367 9057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3162 3751 3884 5792 6745
Size: 325 217 627 1375 589 133 1908 953
Cuts at: 6745 8200 618
Size: 1455 1842
Fragments arranged by size:
1908 1842 1455 1375 953 627 589 325
217 133
BanII G_rGCy'C
Cuts at: 1391 2051 2067 2076 3224 4278 4857 5315 5513
Size: 660 16 9 1148 1054 579 458 198
Cuts at: 5513 5627 5738 6065 6387 6466 6743 1391
Size: 114 111 327 322 79 277 4072
Fragments arranged by size:
4072 1148 1054 660 579 458 327 322
277 198 114 111 79 16 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2930 5643 928
Size: 971 1031 2713 4709
Fragments arranged by size:
4709 2713 1031 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2345 2379 3453 3487 3790 3824 4622
Size: 34 408 34 1074 34 303 34 798
Cuts at: 4622 4656 5607 5641 7615 7649 1903
Size: 34 951 34 1974 34 3678
Fragments arranged by size:
3678 1974 1074 951 798 408 303 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2805 3781 3968 7316 8526 2805
Size: 976 187 3348 1210 3703
Fragments arranged by size:
3703 3348 1210 976 187
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9424
BfrBI ATG'CAT
Cuts at: 2515 2515
Size: 9424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2414 5349
Size: 107 122 71 631 605 538 204 2935
Cuts at: 5349 5971 8035 136
Size: 622 2064 1525
Fragments arranged by size:
2935 2064 1525 631 622 605 538 204
122 107 71
BglII A'GATC_T
Cuts at: 9419 9419
Size: 9424
BlpI GC'TnA_GC
Cuts at: 2243 3003 5078 6064 6841 2243
Size: 760 2075 986 777 4826
Fragments arranged by size:
4826 2075 986 777 760
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2574 3165 3185 3887 4548 5315 5738
Size: 261 523 591 20 702 661 767 423
Cuts at: 5738 6054 6078 6387 7485 8641 1790
Size: 316 24 309 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 767 702 661 591 523
423 316 309 261 24 20
BmgBI CAC'GTC
Cuts at: 1318 2476 1318
Size: 1158 8266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5309 5353 6029
Size: 416 53 83 399 24 3892 44 676
Cuts at: 6029 7155 8113 442
Size: 1126 958 1753
Fragments arranged by size:
3892 1753 1126 958 676 416 399 83
53 44 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3733 3733
Size: 9424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2138 2159 2669 2795 2870 2960 3966
Size: 812 87 21 510 126 75 90 1006
Cuts at: 3966 4509 4736 4910 5090 5393 5446 5801 6511
Size: 543 227 174 180 303 53 355 710
Cuts at: 6511 6602 8084 1239
Size: 91 1482 2579
Fragments arranged by size:
2579 1482 1006 812 710 543 510 355
303 227 180 174 126 91 90 87
75 53 21
Bpu10I CC'TnA_GC
Cuts at: 3705 5138 6330 6365 3705
Size: 1433 1192 35 6764
Fragments arranged by size:
6764 1433 1192 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3327 7549 8414 8712 3327
Size: 4222 865 298 4039
Fragments arranged by size:
4222 4039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6552 8087 882
Size: 617 5053 1535 2219
Fragments arranged by size:
5053 2219 1535 617
BsaAI yAC'GTr
Cuts at: 493 1204 2098 4033 4465 6721 7215 493
Size: 711 894 1935 432 2256 494 2702
Fragments arranged by size:
2702 2256 1935 894 711 494 432
BsaBI GATnn'nnATC
Cuts at: 2993 4583 5055 9418 2993
Size: 1590 472 4363 2999
Fragments arranged by size:
4363 2999 1590 472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2352 3815 7231 7613
Size: 53 83 186 936 819 1463 3416 382
Cuts at: 7613 8248 275
Size: 635 1451
Fragments arranged by size:
3416 1463 1451 936 819 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3842 5516 7857 8255 8529 8676 9087 3842
Size: 1674 2341 398 274 147 411 4179
Fragments arranged by size:
4179 2341 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3292 3322 3964 3994 4910
Size: 30 165 30 1889 30 642 30 916
Cuts at: 4910 4940 5892 5922 1178
Size: 30 952 30 4680
Fragments arranged by size:
4680 1889 952 916 642 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2159 2222 2339 2666 2816 2837 3635
Size: 970 3 63 117 327 150 21 798
Cuts at: 3635 4286 4322 4349 4394 4604 4834 4934 5087
Size: 651 36 27 45 210 230 100 153
Cuts at: 5087 6662 6704 6707 6710 1186
Size: 1575 42 3 3 3900
Fragments arranged by size:
3900 1575 970 798 651 327 230 210
153 150 117 100 63 45 42 36
27 21 3 3 3
BseYI C'CCAG_C
Cuts at: 2247 2359 2392 2422 3249 4702 5155 5548 5641
Size: 112 33 30 827 1453 453 393 93
Cuts at: 5641 5985 6548 8627 9123 2247
Size: 344 563 2079 496 2548
Fragments arranged by size:
2548 2079 1453 827 563 496 453 393
344 112 93 33 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 4652 4686 6195 6632 1163
Size: 573 903 2013 34 1509 437 3955
Fragments arranged by size:
3955 2013 1509 903 573 437 34
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 2344 5059 5517 6874 7635
Size: 275 186 157 186 2715 458 1357 761
Cuts at: 7635 8663 1540
Size: 1028 2301
Fragments arranged by size:
2715 2301 1357 1028 761 458 275 186
186 157
BsiHKAI G_wGCw'C
Cuts at: 2067 2076 2574 3009 6047 6054 6078 6134 6466
Size: 9 498 435 3038 7 24 56 332
Cuts at: 6466 7485 7570 8641 2067
Size: 1019 85 1071 2850
Fragments arranged by size:
3038 2850 1071 1019 498 435 332 85
56 24 9 7
BsmI GAATG_Cn'
Cuts at: 5208 6935 7028 5208
Size: 1727 93 7604
Fragments arranged by size:
7604 1727 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2606 2693 5041 6489 2606
Size: 87 2348 1448 5541
Fragments arranged by size:
5541 2348 1448 87
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2067 2076 2574 3009 3165 3185
Size: 399 261 16 9 498 435 156 20
Cuts at: 3185 3224 3887 4278 4548 4857 5315 5513 5627
Size: 39 663 391 270 309 458 198 114
Cuts at: 5627 5738 6047 6054 6065 6078 6134 6387 6466
Size: 111 309 7 11 13 56 253 79
Cuts at: 6466 6743 7485 7570 8641 1391
Size: 277 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 498 458 435 399
391 309 309 277 270 261 253 198
156 114 111 85 79 56 39 20
16 13 11 9 7
BspEI T'CCGG_A
Cuts at: 9087 9087
Size: 9424
BspHI T'CATG_A
Cuts at: 7313 9043 7313
Size: 1730 7694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2908 3455 4475 6779 844
Size: 2064 547 1020 2304 3489
Fragments arranged by size:
3489 2304 2064 1020 547
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 2827 5189 7311 1394
Size: 429 452 552 2362 2122 3507
Fragments arranged by size:
3507 2362 2122 552 452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6270 6372 7919 8093 65
Size: 1183 5022 102 1547 174 1396
Fragments arranged by size:
5022 1547 1396 1183 174 102
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 2534 2927 3842 5526 8068 8255
Size: 110 347 377 393 915 1684 2542 187
Cuts at: 8255 1700
Size: 2869
Fragments arranged by size:
2869 2542 1684 915 393 377 347 187
110
BsrGI T'GTAC_A
Cuts at: 96 5241 5908 96
Size: 5145 667 3612
Fragments arranged by size:
5145 3612 667
BssHII G'CGCG_C
Cuts at: 1925 2213 2329 2397 2399 2401 2488 2490 2492
Size: 288 116 68 2 2 87 2 2
Cuts at: 2492 2494 2712 2764 2766 1925
Size: 2 218 52 2 8583
Fragments arranged by size:
8583 288 218 116 87 68 52 2
2 2 2 2 2
BssSI C'ACGA_G
Cuts at: 3535 7484 8496 3535
Size: 3949 1012 4463
Fragments arranged by size:
4463 3949 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3467 794
Size: 2673 6751
BstBI TT'CG_AA
Cuts at: 2007 8228 2007
Size: 6221 3203
BstEII G'GTnAC_C
Cuts at: 1184 5489 1184
Size: 4305 5119
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 4362 4997 5101 5619 6524 2260
Size: 2102 635 104 518 905 5160
Fragments arranged by size:
5160 2102 905 635 518 104
BstYI r'GATC_y
Cuts at: 1972 3170 4578 6218 6754 7121 7506 7523 8975
Size: 1198 1408 1640 536 367 385 17 1452
Cuts at: 8975 9061 9073 9419 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1640 1452 1408 1198 536 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2338 2880 3166 4971 6001 6280 6481
Size: 552 1273 542 286 1805 1030 279 201
Cuts at: 6481 513
Size: 3456
Fragments arranged by size:
3456 1805 1273 1030 552 542 286 279
201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4416 487
Size: 683 56 3190 5495
Fragments arranged by size:
5495 3190 683 56
BtsI GCAGTG_nn'
Cuts at: 2253 2585 4674 5457 6940 7734 7754 2253
Size: 332 2089 783 1483 794 20 3923
Fragments arranged by size:
3923 2089 1483 794 783 332 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4771 4806 5335 5370 529
Size: 35 4207 35 529 35 4583
Fragments arranged by size:
4583 4207 529 35 35 35
DraI TTT'AAA
Cuts at: 6728 6728
Size: 9424
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4022 8431 809
Size: 507 2706 4409 1802
Fragments arranged by size:
4409 2706 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2341 2595
Size: 54 1425 50 275 18 325 186 254
Cuts at: 2595 2668 2877 3240 5322 6871 7760 9389 8
Size: 73 209 363 2082 1549 889 1629 43
Fragments arranged by size:
2082 1629 1549 1425 889 363 325 275
254 209 186 73 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2155 2341 6871 1537
Size: 275 343 186 4530 4090
Fragments arranged by size:
4530 4090 343 275 186
EarI CTCTTCn'nnn_
Cuts at: 1353 2691 3202 3579 3725 3778 5775 7354 1353
Size: 1338 511 377 146 53 1997 1579 3423
Fragments arranged by size:
3423 1997 1579 1338 511 377 146 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 2705 4918 8011 8385 8531
Size: 1064 138 690 426 2213 3093 374 146
Cuts at: 8531 387
Size: 1280
Fragments arranged by size:
3093 2213 1280 1064 690 426 374 146
138
EcoICRI GAG'CTC
Cuts at: 2065 2074 6464 2065
Size: 9 4390 5025
Fragments arranged by size:
5025 4390 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2138 2159 2669 2795 2870 2960
Size: 393 419 87 21 510 126 75 90
Cuts at: 2960 3966 3983 4509 4736 4910 5046 5090 5200
Size: 1006 17 526 227 174 136 44 110
Cuts at: 5200 5235 5393 5439 5446 5801 6511 6602 7487
Size: 35 158 46 7 355 710 91 885
Cuts at: 7487 8084 8871 9139 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1006 885 787 710 597 526 510
419 393 355 268 227 174 158 136
126 110 91 90 87 75 46 44
35 21 17 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2564 2915 5498 5832 6333 2141
Size: 423 351 2583 334 501 5232
Fragments arranged by size:
5232 2583 501 423 351 334
EcoO109I rG'GnC_Cy
Cuts at: 2048 2173 2908 3391 4358 4723 5194 5277 5311
Size: 125 735 483 967 365 471 83 34
Cuts at: 5311 5453 5552 5594 5734 5735 5825 6242 6383
Size: 142 99 42 140 1 90 417 141
Cuts at: 6383 2048
Size: 5089
Fragments arranged by size:
5089 967 735 483 471 417 365 142
141 140 125 99 90 83 42 34
1
EcoRI G'AATT_C
Cuts at: 3029 4817 6733 7111 3029
Size: 1788 1916 378 5342
Fragments arranged by size:
5342 1916 1788 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6151 6151
Size: 9424
FspI TGC'GCA
Cuts at: 2367 7930 2367
Size: 5563 3861
HaeII r_GCGC'y
Cuts at: 1678 2314 2393 2420 2446 2660 2732 2747 4709
Size: 636 79 27 26 214 72 15 1962
Cuts at: 4709 5162 8571 1678
Size: 453 3409 2531
Fragments arranged by size:
3409 2531 1962 636 453 214 79 72
27 26 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4194 4226 4233 4265 4547
Size: 32 135 32 2622 32 7 32 282
Cuts at: 4547 4579 4580 4612 4910 4942 5426 5458 5568
Size: 32 1 32 298 32 484 32 110
Cuts at: 5568 5600 6623 6655 8065 8097 8139 8171 1373
Size: 32 1023 32 1410 32 42 32 2626
Fragments arranged by size:
2626 2622 1410 1023 484 298 282 135
110 42 32 32 32 32 32 32
32 32 32 32 32 32 7 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4255 5032 6768 7014 669
Size: 988 281 2317 777 1736 246 3079
Fragments arranged by size:
3079 2317 1736 988 777 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9424
HpaI GTT'AAC
Cuts at: 7014 7014
Size: 9424
KpnI G_GTAC'C
Cuts at: 5796 6749 5796
Size: 953 8471
MfeI C'AATT_G
Cuts at: 3420 7023 3420
Size: 3603 5821
MluI A'CGCG_T
Cuts at: 9300 9300
Size: 9424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2733 3335 8538 8722 1305
Size: 1428 602 5203 184 2007
Fragments arranged by size:
5203 2007 1428 602 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2879 10
Size: 54 1425 343 1047 6555
Fragments arranged by size:
6555 1425 1047 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4031 4829 7382 7741 7900 518
Size: 648 2865 798 2553 359 159 2042
Fragments arranged by size:
2865 2553 2042 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2233 2340 2623 2761 4293 4867 4888
Size: 593 34 107 283 138 1532 574 21
Cuts at: 4888 6320 6840 7517 8665 8910 1606
Size: 1432 520 677 1148 245 2120
Fragments arranged by size:
2120 1532 1432 1148 677 593 574 520
283 245 138 107 34 21
NaeI GCC'GGC
Cuts at: 2159 2159
Size: 9424
NcoI C'CATG_G
Cuts at: 513 1065 2880 6001 513
Size: 552 1815 3121 3936
Fragments arranged by size:
3936 3121 1815 552
NdeI CA'TA_TG
Cuts at: 387 5446 387
Size: 5059 4365
NgoMIV G'CCGG_C
Cuts at: 2157 2157
Size: 9424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9424
NotI GC'GGCC_GC
Cuts at: 2341 6871 2341
Size: 4530 4894
NsiI A_TGCA'T
Cuts at: 2517 2517
Size: 9424
NspI r_CATG'y
Cuts at: 1132 1229 3022 5543 6782 8327 1132
Size: 97 1793 2521 1239 1545 2229
Fragments arranged by size:
2521 2229 1793 1545 1239 97
PasI CC'CwG_GG
Cuts at: 1348 4362 5308 1348
Size: 3014 946 5464
Fragments arranged by size:
5464 3014 946
PciI A'CATG_T
Cuts at: 8323 8323
Size: 9424
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3317 4740 5162 5636 6007 6625 1108
Size: 2209 1423 422 474 371 618 3907
Fragments arranged by size:
3907 2209 1423 618 474 422 371
PfoI T'CCnGG_A
Cuts at: 2067 2136 6488 2067
Size: 69 4352 5003
Fragments arranged by size:
5003 4352 69
PmeI GTTT'AAAC
Cuts at: 6728 6728
Size: 9424
PmlI CAC'GTG
Cuts at: 4465 4465
Size: 9424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3324 3578 3610 3857 3889 7493 7525 9006
Size: 32 254 32 247 32 3604 32 1481
Cuts at: 9006 9038 3292
Size: 32 3678
Fragments arranged by size:
3678 3604 1481 254 247 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2908 3391 4723 5453 5552 6242 2908
Size: 483 1332 730 99 690 6090
Fragments arranged by size:
6090 1332 730 690 483 99
PshAI GACnn'nnGTC
Cuts at: 1613 5496 5804 6289 1613
Size: 3883 308 485 4748
Fragments arranged by size:
4748 3883 485 308
PsiI TTA'TAA
Cuts at: 6994 6994
Size: 9424
PspOMI G'GGCC_C
Cuts at: 2047 5311 5734 6383 2047
Size: 3264 423 649 5088
Fragments arranged by size:
5088 3264 649 423
PspXI vC'TCGA_Gb
Cuts at: 2411 2411
Size: 9424
PstI C_TGCA'G
Cuts at: 830 2043 2310 2385 2436 2919 5464 5659 5699
Size: 1213 267 75 51 483 2545 195 40
Cuts at: 5699 5752 6776 830
Size: 53 1024 3478
Fragments arranged by size:
3478 2545 1213 1024 483 267 195 75
53 51 40
PvuI CG_AT'CG
Cuts at: 2001 5059 2001
Size: 3058 6366
PvuII CAG'CTG
Cuts at: 2233 2623 2761 4867 4888 2233
Size: 390 138 2106 21 6769
Fragments arranged by size:
6769 2106 390 138 21
SacI G_AGCT'C
Cuts at: 2067 2076 6466 2067
Size: 9 4390 5025
Fragments arranged by size:
5025 4390 9
SacII CC_GC'GG
Cuts at: 2341 2341
Size: 9424
SalI G'TCGA_C
Cuts at: 1936 6766 1936
Size: 4830 4594
SanDI GG'GwC_CC
Cuts at: 5552 5552
Size: 9424
SapI GCTCTTCn'nnn_
Cuts at: 3778 3778
Size: 9424
SbfI CC_TGCA'GG
Cuts at: 2919 6776 2919
Size: 3857 5567
ScaI AGT'ACT
Cuts at: 7672 9384 7672
Size: 1712 7712
SfcI C'TryA_G
Cuts at: 826 1045 2039 2306 2381 2432 2915 3068 5460
Size: 219 994 267 75 51 483 153 2392
Cuts at: 5460 5655 5695 5748 6246 6772 7907 8588 8779
Size: 195 40 53 498 526 1135 681 191
Cuts at: 8779 826
Size: 1471
Fragments arranged by size:
2392 1471 1135 994 681 526 498 483
267 219 195 191 153 75 53 51
40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9424
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9424
SmaI CCC'GGG
Cuts at: 1872 4540 5234 6751 1872
Size: 2668 694 1517 4545
Fragments arranged by size:
4545 2668 1517 694
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 3342 7528 8429 8691 820
Size: 197 926 468 931 4186 901 262 1553
Fragments arranged by size:
4186 1553 931 926 901 468 262 197
SnaBI TAC'GTA
Cuts at: 493 6721 7215 493
Size: 6228 494 2702
Fragments arranged by size:
6228 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9424
SphI G_CATG'C
Cuts at: 1132 6782 1132
Size: 5650 3774
SspI AAT'ATT
Cuts at: 5 52 7348 5
Size: 47 7296 2081
Fragments arranged by size:
7296 2081 47
StuI AGG'CCT
Cuts at: 1517 2451 6542 1517
Size: 934 4091 4399
Fragments arranged by size:
4399 4091 934
StyI C'CwwG_G
Cuts at: 513 1065 2874 2880 4692 4848 5590 6001 6863
Size: 552 1809 6 1812 156 742 411 862
Cuts at: 6863 513
Size: 3074
Fragments arranged by size:
3074 1812 1809 862 742 552 411 156
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 5879 5999 7451 7468 7621 7806
Size: 1255 771 3528 120 1452 17 153 185
Cuts at: 7806 8251 325
Size: 445 1498
Fragments arranged by size:
3528 1498 1452 1255 771 445 185 153
120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5241 5908 7670
Size: 274 80 33 51 1443 3264 667 1762
Cuts at: 7670 9382 96
Size: 1712 138
Fragments arranged by size:
3264 1762 1712 1443 667 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3964 3996 5536 5568 5746 5778 9384 9416 3964
Size: 32 1540 32 178 32 3606 32 3972
Fragments arranged by size:
3972 3606 1540 178 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6240 1316
Size: 4924 4500
XbaI T'CTAG_A
Cuts at: 3763 6679 6760 3763
Size: 2916 81 6427
Fragments arranged by size:
6427 2916 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4997 5464 4997
Size: 467 8957
XhoI C'TCGA_G
Cuts at: 1943 2411 1943
Size: 468 8956
XmaI C'CCGG_G
Cuts at: 1870 4538 5232 6749 1870
Size: 2668 694 1517 4545
Fragments arranged by size:
4545 2668 1517 694
XmnI GAAnn'nnTTC
Cuts at: 3353 3593 3968 4899 7553 3353
Size: 240 375 931 2654 5224
Fragments arranged by size:
5224 2654 931 375 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7232 276
Size: 53 83 186 936 5698 2468
Fragments arranged by size:
5698 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NarI NruI PacI
PsrI RsrII SexAI SfiI SfoI SrfI SwaI