Restriction map for
pFN21ASDA0352
(Circular) (Six-base) MAPSORT of: pFN21ASDA0352.seq Check: 9563 from: 1 to: 6865
pFN21ASDA0352 6865 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 4675 278
Size: 53 83 186 936 582 2557 2468
Fragments arranged by size:
2557 2468 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 2823 4208 1937
Size: 346 540 1385 4594
Fragments arranged by size:
4594 1385 540 346
Acc65I G'GTAC_C
Cuts at: 2450 4186 2450
Size: 1736 5129
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2274 2770 4928 6312 6580 1632
Size: 461 181 496 2158 1384 268 1917
Fragments arranged by size:
2158 1917 1384 496 461 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6668
AflIII A'CryG_T
Cuts at: 1203 3231 4057 5764 6741 1203
Size: 2028 826 1707 977 1327
Fragments arranged by size:
2028 1707 1327 977 826
AgeI A'CCGG_T
Cuts at: 5696 5696
Size: 6865
AhdI GACnn_n'nnGTC
Cuts at: 5594 5594
Size: 6865
AleI CACnn'nnGTG
Cuts at: 1166 2123 1166
Size: 957 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6865
AlwNI CAG_nnn'CTG
Cuts at: 2477 3474 3674 4608 6180 2477
Size: 997 200 934 1572 3162
Fragments arranged by size:
3162 1572 997 934 200
ApaI G_GGCC'C
Cuts at: 3997 3997
Size: 6865
ApaLI G'TGCA_C
Cuts at: 3608 4067 4922 6078 3608
Size: 459 855 1156 4395
Fragments arranged by size:
4395 1156 855 459
ApoI r'AATT_y
Cuts at: 1493 3878 4174 4400 4552 6661 1493
Size: 2385 296 226 152 2109 1697
Fragments arranged by size:
2385 2109 1697 296 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5419 160
Size: 872 4387 1606
Fragments arranged by size:
4387 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 2909 3111 3180 3988 4190 1870
Size: 73 966 202 69 808 202 4545
Fragments arranged by size:
4545 966 808 202 202 73 69
AvrII C'CTAG_G
Cuts at: 2710 2710
Size: 6865
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2434 2467 2434
Size: 33 6832
BamHI G'GATC_C
Cuts at: 3897 4195 4562 3897
Size: 298 367 6200
Fragments arranged by size:
6200 367 298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 4186 5641 618
Size: 325 217 627 663 1736 1455 1842
Fragments arranged by size:
1842 1736 1455 663 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2750 2904 3084 3997 4184 1391
Size: 1359 154 180 913 187 4072
Fragments arranged by size:
4072 1359 913 187 180 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2658 2949 3535 3630 928
Size: 971 759 291 586 95 4163
Fragments arranged by size:
4163 971 759 586 291 95
BbvCI CC'TCA_GC
Cuts at: 2952 2952
Size: 6865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3092 3126 5056 5090 1903
Size: 34 1155 34 1930 34 3678
Fragments arranged by size:
3678 1930 1155 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4757 5967 4757
Size: 1210 5655
BclI T'GATC_A
Cuts at: 1548 2310 1548
Size: 762 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 5476 136
Size: 107 122 71 631 605 484 3320 1525
Fragments arranged by size:
3320 1525 631 605 484 122 107 71
BglII A'GATC_T
Cuts at: 6860 6860
Size: 6865
BlpI GC'TnA_GC
Cuts at: 2340 4282 2340
Size: 1942 4923
Bme1580I G_kGCm'C
Cuts at: 1790 3612 3997 4026 4071 4926 6082 1790
Size: 1822 385 29 45 855 1156 2573
Fragments arranged by size:
2573 1822 1156 855 385 45 29
BmgBI CAC'GTC
Cuts at: 1318 2679 3691 1318
Size: 1361 1012 4492
Fragments arranged by size:
4492 1361 1012
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 2541 2844
Size: 416 53 83 399 24 800 324 303
Cuts at: 2844 3250 4596 5554 442
Size: 406 1346 958 1753
Fragments arranged by size:
1753 1346 958 800 416 406 399 324
303 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 6865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2417 2577 2888 3743 5525 1239
Size: 1112 66 160 311 855 1782 2579
Fragments arranged by size:
2579 1782 1112 855 311 160 66
Bpu10I CC'TnA_GC
Cuts at: 2952 2952
Size: 6865
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4990 5855 6153 4990
Size: 865 298 5702
Fragments arranged by size:
5702 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 3149 3350 5528 882
Size: 617 597 1053 201 2178 2219
Fragments arranged by size:
2219 2178 1053 617 597 201
BsaAI yAC'GTr
Cuts at: 493 1204 4162 4656 493
Size: 711 2958 494 2702
Fragments arranged by size:
2958 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6859 6859
Size: 6865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3627 4672 5054
Size: 53 83 186 936 582 1512 1045 382
Cuts at: 5054 5689 275
Size: 635 1451
Fragments arranged by size:
1512 1451 1045 936 635 582 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5298 5696 5970 6117 6528 5298
Size: 398 274 147 411 5635
Fragments arranged by size:
5635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2653 2683 3161 3191 1178
Size: 30 165 30 1250 30 478 30 4852
Fragments arranged by size:
4852 1250 478 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2987 3797 1186
Size: 1168 633 810 4254
Fragments arranged by size:
4254 1168 810 633
BseYI C'CCAG_C
Cuts at: 2700 3340 3751 6068 6564 2700
Size: 640 411 2317 496 3001
Fragments arranged by size:
3001 2317 640 496 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2193 3670 3994 1163
Size: 573 457 1477 324 4034
Fragments arranged by size:
4034 1477 573 457 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4315 5076 6104 1540
Size: 275 186 2314 761 1028 2301
Fragments arranged by size:
2314 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2405 2750 2868 2904 3612 4071 4926 5011 6082
Size: 345 118 36 708 459 855 85 1071
Cuts at: 6082 2405
Size: 3188
Fragments arranged by size:
3188 1071 855 708 459 345 118 85
36
BsmI GAATG_Cn'
Cuts at: 4376 4469 4376
Size: 93 6772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2750 2868 2904 3084 3612 3997
Size: 399 615 345 118 36 180 528 385
Cuts at: 3997 4026 4071 4184 4926 5011 6082 1391
Size: 29 45 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 528 399 385 345
180 118 113 85 45 36 29
BspEI T'CCGG_A
Cuts at: 6528 6528
Size: 6865
BspHI T'CATG_A
Cuts at: 4047 4754 6484 4047
Size: 707 1730 4428
Fragments arranged by size:
4428 1730 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2485 3135 4220 844
Size: 1232 409 650 1085 3489
Fragments arranged by size:
3489 1232 1085 650 409
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 3957 4752 1394
Size: 429 316 1818 795 3507
Fragments arranged by size:
3507 1818 795 429 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 2782 5360 5534 65
Size: 1183 1534 2578 174 1396
Fragments arranged by size:
2578 1534 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 3891 5509 5696 1700
Size: 110 281 1800 1618 187 2869
Fragments arranged by size:
2869 1800 1618 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 4116 96
Size: 4020 2845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 2743 4925 5937 2743
Size: 2182 1012 3671
Fragments arranged by size:
3671 2182 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2477 3150 3474 794
Size: 1683 673 324 4185
Fragments arranged by size:
4185 1683 673 324
BstBI TT'CG_AA
Cuts at: 2007 5669 2007
Size: 3662 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6865
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2489 2128
Size: 361 6504
BstYI r'GATC_y
Cuts at: 1972 2875 3897 4195 4562 4947 4964 6416 6502
Size: 903 1022 298 367 385 17 1452 86
Cuts at: 6502 6514 6860 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1022 903 385 367 346 298
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5356
Fragments arranged by size:
5356 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6126
Fragments arranged by size:
6126 683 56
BtsI GCAGTG_nn'
Cuts at: 2403 3475 3637 4381 5175 5195 2403
Size: 1072 162 744 794 20 4073
Fragments arranged by size:
4073 1072 794 744 162 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3832 3867 529
Size: 35 3268 35 3527
Fragments arranged by size:
3527 3268 35 35
DraI TTT'AAA
Cuts at: 4169 4169
Size: 6865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5872 809
Size: 507 4556 1802
Fragments arranged by size:
4556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3402 4312 5201
Size: 54 1425 50 275 18 1572 910 889
Cuts at: 5201 6830 8
Size: 1629 43
Fragments arranged by size:
1629 1572 1425 910 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4312 1537
Size: 275 2500 4090
Fragments arranged by size:
4090 2500 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2303 2571 3638 3792 4795 1353
Size: 848 102 268 1067 154 1003 3423
Fragments arranged by size:
3423 1067 1003 848 268 154 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4154 5452 5826 5972 387
Size: 1064 138 2321 244 1298 374 146 1280
Fragments arranged by size:
2321 1298 1280 1064 374 244 146 138
EcoICRI GAG'CTC
Cuts at: 2748 2902 2748
Size: 154 6711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2274 2351 2417 2577 2770 2888
Size: 393 461 181 77 66 160 193 118
Cuts at: 2888 3743 4928 5525 6312 6580 1239
Size: 855 1185 597 787 268 1524
Fragments arranged by size:
1524 1185 855 787 597 461 393 268
193 181 160 118 77 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2366 2072
Size: 294 6571
EcoO109I rG'GnC_Cy
Cuts at: 2226 2359 2914 3993 3994 2226
Size: 133 555 1079 1 5097
Fragments arranged by size:
5097 1079 555 133 1
EcoRI G'AATT_C
Cuts at: 3878 4174 4552 3878
Size: 296 378 6191
Fragments arranged by size:
6191 378 296
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 3809 6012 1678
Size: 2131 2203 2531
Fragments arranged by size:
2531 2203 2131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3161 3193 5506 5538 5580
Size: 32 135 32 1589 32 2313 32 42
Cuts at: 5580 5612 1373
Size: 32 2626
Fragments arranged by size:
2626 2313 1589 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6865
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KpnI G_GTAC'C
Cuts at: 2454 4190 2454
Size: 1736 5129
MfeI C'AATT_G
Cuts at: 4464 4464
Size: 6865
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5979 6163 1305
Size: 4674 184 2007
Fragments arranged by size:
4674 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3404 10
Size: 54 1425 343 1572 3471
Fragments arranged by size:
3471 1572 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2123 2126 3605 3922 4062 4823
Size: 648 861 96 3 1479 317 140 761
Cuts at: 4823 5182 5341 518
Size: 359 159 2042
Fragments arranged by size:
2042 1479 861 761 648 359 317 159
140 96 3
MspA1I CmG'CkG
Cuts at: 1606 2177 3265 4008 4281 4958 6106 6351 1606
Size: 571 1088 743 273 677 1148 245 2120
Fragments arranged by size:
2120 1148 1088 743 677 571 273 245
NaeI GCC'GGC
Cuts at: 2093 2093
Size: 6865
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5356
Fragments arranged by size:
5356 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6865
NgoMIV G'CCGG_C
Cuts at: 2091 2091
Size: 6865
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6865
NotI GC'GGCC_GC
Cuts at: 4312 4312
Size: 6865
NspI r_CATG'y
Cuts at: 1132 1229 3110 3235 4061 4223 5768 1132
Size: 97 1881 125 826 162 1545 2229
Fragments arranged by size:
2229 1881 1545 826 162 125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6865
PciI A'CATG_T
Cuts at: 3231 4057 5764 3231
Size: 826 1707 4332
Fragments arranged by size:
4332 1707 826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3619 1108
Size: 2511 4354
PfoI T'CCnGG_A
Cuts at: 3165 3165
Size: 6865
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 6447 6479 4934
Size: 32 1481 32 5320
Fragments arranged by size:
5320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2226 2359 2914 2226
Size: 133 555 6177
Fragments arranged by size:
6177 555 133
PshAI GACnn'nnGTC
Cuts at: 1613 3483 1613
Size: 1870 4995
PsiI TTA'TAA
Cuts at: 4435 4435
Size: 6865
PspOMI G'GGCC_C
Cuts at: 3993 3993
Size: 6865
PspXI vC'TCGA_Gb
Cuts at: 2909 2909
Size: 6865
PstI C_TGCA'G
Cuts at: 830 3470 3503 3747 3813 4217 830
Size: 2640 33 244 66 404 3478
Fragments arranged by size:
3478 2640 404 244 66 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 2177 3265 2177
Size: 1088 5777
SacI G_AGCT'C
Cuts at: 2750 2904 2750
Size: 154 6711
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SapI GCTCTTCn'nnn_
Cuts at: 3792 3792
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 4217 4217
Size: 6865
ScaI AGT'ACT
Cuts at: 5113 6825 5113
Size: 1712 5153
SfcI C'TryA_G
Cuts at: 826 1045 3466 3499 3743 3809 4027 4213 5348
Size: 219 2421 33 244 66 218 186 1135
Cuts at: 5348 6029 6220 826
Size: 681 191 1471
Fragments arranged by size:
2421 1471 1135 681 244 219 218 191
186 66 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 3891 1700
Size: 2191 4674
SmaI CCC'GGG
Cuts at: 1872 3182 4192 1872
Size: 1310 1010 4545
Fragments arranged by size:
4545 1310 1010
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 4969 5870 6132 820
Size: 197 926 966 2060 901 262 1553
Fragments arranged by size:
2060 1553 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4162 4656 493
Size: 3669 494 2702
Fragments arranged by size:
3669 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6865
SphI G_CATG'C
Cuts at: 1132 3110 4223 1132
Size: 1978 1113 3774
Fragments arranged by size:
3774 1978 1113
SspI AAT'ATT
Cuts at: 5 52 3291 4789 5
Size: 47 3239 1498 2081
Fragments arranged by size:
3239 2081 1498 47
StuI AGG'CCT
Cuts at: 1517 2372 3464 1517
Size: 855 1092 4918
Fragments arranged by size:
4918 1092 855
StyI C'CwwG_G
Cuts at: 513 1065 2022 2488 2518 2710 4304 513
Size: 552 957 466 30 192 1594 3074
Fragments arranged by size:
3074 1594 957 552 466 192 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3186 3765 3782 4892 4909 5062 5247
Size: 1255 1606 579 17 1110 17 153 185
Cuts at: 5247 5692 325
Size: 445 1498
Fragments arranged by size:
1606 1498 1255 1110 579 445 185 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4116 5111 6823
Size: 274 80 33 51 1443 2139 995 1712
Cuts at: 6823 96
Size: 138
Fragments arranged by size:
2139 1712 1443 995 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2653 2668 2685 2700 3506 3538 4016 4048 6825
Size: 15 17 15 806 32 478 32 2777
Cuts at: 6825 6857 2653
Size: 32 2661
Fragments arranged by size:
2777 2661 806 478 32 32 32 17
15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6865
XbaI T'CTAG_A
Cuts at: 3076 3589 4201 3076
Size: 513 612 5740
Fragments arranged by size:
5740 612 513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 6865
XhoI C'TCGA_G
Cuts at: 1943 2909 1943
Size: 966 5899
XmaI C'CCGG_G
Cuts at: 1870 3180 4190 1870
Size: 1310 1010 4545
Fragments arranged by size:
4545 1310 1010
XmnI GAAnn'nnTTC
Cuts at: 2195 4994 2195
Size: 2799 4066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 4673 276
Size: 53 83 186 936 582 2557 2468
Fragments arranged by size:
2557 2468 936 582 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI