Restriction map for
pFN21ASDA0334
(Circular) (Six-base) MAPSORT of: pFN21ASDA0334.seq Check: 7769 from: 1 to: 7267
pFN21ASDA0334 7267 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5077 278
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4610 1937
Size: 2673 4594
Acc65I G'GTAC_C
Cuts at: 4195 4588 4195
Size: 393 6874
AclI AA'CG_TT
Cuts at: 1563 5394 5767 1563
Size: 3831 373 3063
Fragments arranged by size:
3831 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 3059 3671 4311 5330 6714 6982 1632
Size: 1056 371 612 640 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1056 1019 640 612 371 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7070
AflIII A'CryG_T
Cuts at: 1203 6166 7143 1203
Size: 4963 977 1327
Fragments arranged by size:
4963 1327 977
AgeI A'CCGG_T
Cuts at: 6098 6098
Size: 7267
AhdI GACnn_n'nnGTC
Cuts at: 5996 5996
Size: 7267
AleI CACnn'nnGTG
Cuts at: 1166 4016 1166
Size: 2850 4417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7267
AlwNI CAG_nnn'CTG
Cuts at: 3221 3709 5010 6582 3221
Size: 488 1301 1572 3906
Fragments arranged by size:
3906 1572 1301 488
ApaLI G'TGCA_C
Cuts at: 2821 5324 6480 2821
Size: 2503 1156 3608
Fragments arranged by size:
3608 2503 1156
ApoI r'AATT_y
Cuts at: 1493 2307 2597 2663 2674 2980 3109 3478 3532
Size: 814 290 66 11 306 129 369 54
Cuts at: 3532 3672 3745 3775 4576 4802 4954 7063 1493
Size: 140 73 30 801 226 152 2109 1697
Fragments arranged by size:
2109 1697 814 801 369 306 290 226
152 140 129 73 66 54 30 11
AseI AT'TA_AT
Cuts at: 160 1032 2360 5821 160
Size: 872 1328 3461 1606
Fragments arranged by size:
3461 1606 1328 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
AvaI C'yCGr_G
Cuts at: 1870 1943 4592 1870
Size: 73 2649 4545
Fragments arranged by size:
4545 2649 73
BamHI G'GATC_C
Cuts at: 2195 4597 4964 2195
Size: 2402 367 4498
Fragments arranged by size:
4498 2402 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 4195 4588 6043 618
Size: 325 217 627 1112 1296 393 1455 1842
Fragments arranged by size:
1842 1455 1296 1112 627 393 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 4307 4586 1391
Size: 666 2250 279 4072
Fragments arranged by size:
4072 2250 666 279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5458 5492 1903
Size: 34 3521 34 3678
Fragments arranged by size:
3678 3521 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2707 3388 5159 6369 2707
Size: 681 1771 1210 3605
Fragments arranged by size:
3605 1771 1210 681
BclI T'GATC_A
Cuts at: 1548 3295 1548
Size: 1747 5520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5878 136
Size: 107 122 71 631 605 4206 1525
Fragments arranged by size:
4206 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7262 7262
Size: 7267
BlpI GC'TnA_GC
Cuts at: 4350 4684 4350
Size: 334 6933
Bme1580I G_kGCm'C
Cuts at: 1790 2825 2902 3951 5328 6484 1790
Size: 1035 77 1049 1377 1156 2573
Fragments arranged by size:
2573 1377 1156 1049 1035 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7267
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2201 3478 3553
Size: 416 53 83 399 24 784 1277 75
Cuts at: 3553 3966 4998 5956 442
Size: 413 1032 958 1753
Fragments arranged by size:
1753 1277 1032 958 784 416 413 399
83 75 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5927 1239
Size: 4688 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3257 3507 4119 5392 6257 6555 3257
Size: 250 612 1273 865 298 3969
Fragments arranged by size:
3969 1273 865 612 298 250
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5930 882
Size: 617 4431 2219
Fragments arranged by size:
4431 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4564 5058 493
Size: 711 3360 494 2702
Fragments arranged by size:
3360 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2958 7261 2958
Size: 4303 2964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5074 5456 6091 275
Size: 53 83 186 936 3541 382 635 1451
Fragments arranged by size:
3541 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5700 6098 6372 6519 6930 5700
Size: 398 274 147 411 6037
Fragments arranged by size:
6037 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3388 3418 1178
Size: 30 165 30 1985 30 5027
Fragments arranged by size:
5027 1985 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7267
BseYI C'CCAG_C
Cuts at: 4359 6470 6966 4359
Size: 2111 496 4660
Fragments arranged by size:
4660 2111 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 1163
Size: 573 535 6159
Fragments arranged by size:
6159 573 535
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3354 4717 5478 6506 1540
Size: 275 186 1353 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1353 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2057 2825 3003 3404 3989 4307 4457 5328 5413
Size: 768 178 401 585 318 150 871 85
Cuts at: 5413 6484 2057
Size: 1071 2840
Fragments arranged by size:
2840 1071 871 768 585 401 318 178
150 85
BsmI GAATG_Cn'
Cuts at: 4778 4871 4778
Size: 93 7174
BsmBI CGTCTCn'nnnn_
Cuts at: 3361 3361
Size: 7267
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2825 2902 3003 3404 3951 3989
Size: 399 267 768 77 101 401 547 38
Cuts at: 3989 4307 4457 4586 5328 5413 6484 1391
Size: 318 150 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 768 742 547 401 399 318
267 150 129 101 85 77 38
BspEI T'CCGG_A
Cuts at: 6930 6930
Size: 7267
BspHI T'CATG_A
Cuts at: 2397 2995 3430 5156 6886 2397
Size: 598 435 1726 1730 2778
Fragments arranged by size:
2778 1730 1726 598 435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4622 844
Size: 3778 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5154 1394
Size: 429 3331 3507
Fragments arranged by size:
3507 3331 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5762 5936 65
Size: 1183 4514 174 1396
Fragments arranged by size:
4514 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4512 5911 6098 1700
Size: 110 2702 1399 187 2869
Fragments arranged by size:
2869 2702 1399 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7267
BssSI C'ACGA_G
Cuts at: 5327 6339 5327
Size: 1012 6255
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3007 794
Size: 2213 5054
BstBI TT'CG_AA
Cuts at: 2007 6071 2007
Size: 4064 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7267
BstXI CCAn_nnnn'nTGG
Cuts at: 3239 3491 3239
Size: 252 7015
BstYI r'GATC_y
Cuts at: 1972 2195 3384 4597 4964 5349 5366 6818 6904
Size: 223 1189 1213 367 385 17 1452 86
Cuts at: 6904 6916 7262 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1213 1189 385 367 346 223
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3697 4399 513
Size: 552 2632 702 3381
Fragments arranged by size:
3381 2632 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6528
Fragments arranged by size:
6528 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 4783 5577 5597 2283
Size: 2500 794 20 3953
Fragments arranged by size:
3953 2500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7232
DraI TTT'AAA
Cuts at: 2577 2688 4571 2577
Size: 111 1883 5273
Fragments arranged by size:
5273 1883 111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6274 809
Size: 507 4958 1802
Fragments arranged by size:
4958 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3351 4714 5603
Size: 54 1425 50 275 18 1521 1363 889
Cuts at: 5603 7232 8
Size: 1629 43
Fragments arranged by size:
1629 1521 1425 1363 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3351 4714 1537
Size: 275 1539 1363 4090
Fragments arranged by size:
4090 1539 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2707 2838 3192 3210 3325 5197 1353
Size: 987 367 131 354 18 115 1872 3423
Fragments arranged by size:
3423 1872 987 367 354 131 115 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5854 6228 6374 387
Size: 1064 138 4265 374 146 1280
Fragments arranged by size:
4265 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2055 4305 2055
Size: 2250 5017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 3059 3671 4311 5330 5927 6714
Size: 393 1056 371 612 640 1019 597 787
Cuts at: 6714 6982 1239
Size: 268 1524
Fragments arranged by size:
1524 1056 1019 787 640 612 597 393
371 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3050 3050
Size: 7267
EcoRI G'AATT_C
Cuts at: 2597 3109 4576 4954 2597
Size: 512 1467 378 4910
Fragments arranged by size:
4910 1467 512 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3221 3701 3221
Size: 480 6787
FspI TGC'GCA
Cuts at: 5773 5773
Size: 7267
HaeII r_GCGC'y
Cuts at: 1678 6414 1678
Size: 4736 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 3227 3259 3353
Size: 32 135 32 1265 32 358 32 94
Cuts at: 3353 3385 4219 4251 5908 5940 5982 6014 1373
Size: 32 834 32 1657 32 42 32 2626
Fragments arranged by size:
2626 1657 1265 834 358 135 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2065 3929 4142 4611 4857 669
Size: 988 281 127 1864 213 469 246 3079
Fragments arranged by size:
3079 1864 988 469 281 246 213 127
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7267
HpaI GTT'AAC
Cuts at: 4857 4857
Size: 7267
KpnI G_GTAC'C
Cuts at: 4199 4592 4199
Size: 393 6874
MfeI C'AATT_G
Cuts at: 3608 4866 3608
Size: 1258 6009
MluI A'CGCG_T
Cuts at: 7143 7143
Size: 7267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6381 6565 1305
Size: 5076 184 2007
Fragments arranged by size:
5076 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5445
Fragments arranged by size:
5445 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2967 4016 5225 5584 5743 518
Size: 648 1801 1049 1209 359 159 2042
Fragments arranged by size:
2042 1801 1209 1049 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3218 4354 4683 5360 6508 6753 1606
Size: 1612 1136 329 677 1148 245 2120
Fragments arranged by size:
2120 1612 1148 1136 677 329 245
NaeI GCC'GGC
Cuts at: 4514 4514
Size: 7267
NcoI C'CATG_G
Cuts at: 513 1065 3697 4399 513
Size: 552 2632 702 3381
Fragments arranged by size:
3381 2632 702 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7267
NgoMIV G'CCGG_C
Cuts at: 4512 4512
Size: 7267
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7267
NotI GC'GGCC_GC
Cuts at: 4714 4714
Size: 7267
NspI r_CATG'y
Cuts at: 1132 1229 2614 3556 4625 6170 1132
Size: 97 1385 942 1069 1545 2229
Fragments arranged by size:
2229 1545 1385 1069 942 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7267
PciI A'CATG_T
Cuts at: 6166 6166
Size: 7267
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7267
PmeI GTTT'AAAC
Cuts at: 4571 4571
Size: 7267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5336 5368 6849 6881 5336
Size: 32 1481 32 5722
Fragments arranged by size:
5722 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7267
PsiI TTA'TAA
Cuts at: 2523 4837 2523
Size: 2314 4953
PstI C_TGCA'G
Cuts at: 830 2247 3714 4036 4096 4619 830
Size: 1417 1467 322 60 523 3478
Fragments arranged by size:
3478 1467 1417 523 322 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7267
PvuII CAG'CTG
Cuts at: 3218 4354 3218
Size: 1136 6131
SacI G_AGCT'C
Cuts at: 2057 4307 2057
Size: 2250 5017
SalI G'TCGA_C
Cuts at: 1936 4609 1936
Size: 2673 4594
SbfI CC_TGCA'GG
Cuts at: 4619 4619
Size: 7267
ScaI AGT'ACT
Cuts at: 4204 5515 7227 4204
Size: 1311 1712 4244
Fragments arranged by size:
4244 1712 1311
SfcI C'TryA_G
Cuts at: 826 1045 2041 2243 3144 3710 4032 4092 4173
Size: 219 996 202 901 566 322 60 81
Cuts at: 4173 4615 5750 6431 6622 826
Size: 442 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 901 681 566 442 322
219 202 191 81 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7267
SmaI CCC'GGG
Cuts at: 1872 4594 1872
Size: 2722 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3272 3522 4134 5371 6272 6534
Size: 197 926 1329 250 612 1237 901 262
Cuts at: 6534 820
Size: 1553
Fragments arranged by size:
1553 1329 1237 926 901 612 262 250
197
SnaBI TAC'GTA
Cuts at: 493 4564 5058 493
Size: 4071 494 2702
Fragments arranged by size:
4071 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7267
SphI G_CATG'C
Cuts at: 1132 4625 1132
Size: 3493 3774
SspI AAT'ATT
Cuts at: 5 52 2573 3643 5191 5
Size: 47 2521 1070 1548 2081
Fragments arranged by size:
2521 2081 1548 1070 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7267
StyI C'CwwG_G
Cuts at: 513 1065 3697 4399 4545 4706 513
Size: 552 2632 702 146 161 3074
Fragments arranged by size:
3074 2632 702 552 161 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2920 3424 4097 5294 5311 5464 5649
Size: 1255 1340 504 673 1197 17 153 185
Cuts at: 5649 6094 325
Size: 445 1498
Fragments arranged by size:
1498 1340 1255 1197 673 504 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3134 3911 3937
Size: 274 80 33 51 1443 1157 777 26
Cuts at: 3937 4202 5513 7225 96
Size: 265 1311 1712 138
Fragments arranged by size:
1712 1443 1311 1157 777 274 265 138
80 51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7227 7259 7227
Size: 32 7235
Tth111I GACn'n_nGTC
Cuts at: 1316 4273 1316
Size: 2957 4310
XbaI T'CTAG_A
Cuts at: 2135 2855 2887 4603 2135
Size: 720 32 1716 4799
Fragments arranged by size:
4799 1716 720 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3845 3845
Size: 7267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7267
XmaI C'CCGG_G
Cuts at: 1870 4592 1870
Size: 2722 4545
XmnI GAAnn'nnTTC
Cuts at: 3684 5396 3684
Size: 1712 5555
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5075 276
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI