Restriction map for
pFN21ASDA0331
(Circular) (Six-base) MAPSORT of: pFN21ASDA0331.seq Check: 2707 from: 1 to: 7054
pFN21ASDA0331 7054 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3372 4864 278
Size: 53 83 186 936 1836 1492 2468
Fragments arranged by size:
2468 1836 1492 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 1937
Size: 2460 4594
Acc65I G'GTAC_C
Cuts at: 4375 4375
Size: 7054
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3452 3798 5117 6501 6769 1632
Size: 1820 346 1319 1384 268 1917
Fragments arranged by size:
1917 1820 1384 1319 346 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6857
AflIII A'CryG_T
Cuts at: 1203 3057 3582 5953 6930 1203
Size: 1854 525 2371 977 1327
Fragments arranged by size:
2371 1854 1327 977 525
AgeI A'CCGG_T
Cuts at: 5885 5885
Size: 7054
AhdI GACnn_n'nnGTC
Cuts at: 2088 5783 2088
Size: 3695 3359
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7054
AlwNI CAG_nnn'CTG
Cuts at: 3968 4797 6369 3968
Size: 829 1572 4653
Fragments arranged by size:
4653 1572 829
ApaLI G'TGCA_C
Cuts at: 4222 5111 6267 4222
Size: 889 1156 5009
Fragments arranged by size:
5009 1156 889
ApoI r'AATT_y
Cuts at: 1493 2699 4157 4363 4589 4741 6850 1493
Size: 1206 1458 206 226 152 2109 1697
Fragments arranged by size:
2109 1697 1458 1206 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 3618 4379 1870
Size: 73 1675 761 4545
Fragments arranged by size:
4545 1675 761 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3239 3272 3239
Size: 33 7021
BamHI G'GATC_C
Cuts at: 2479 3481 4384 4751 2479
Size: 1002 903 367 4782
Fragments arranged by size:
4782 1002 903 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4375 5830 618
Size: 325 217 627 2588 1455 1842
Fragments arranged by size:
2588 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 4373 1391
Size: 751 2231 4072
Fragments arranged by size:
4072 2231 751
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2739 3908 928
Size: 971 840 1169 4074
Fragments arranged by size:
4074 1169 971 840
BbvCI CC'TCA_GC
Cuts at: 2264 3351 2264
Size: 1087 5967
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5245 5279 1903
Size: 34 3308 34 3678
Fragments arranged by size:
3678 3308 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7054
BfrBI ATG'CAT
Cuts at: 2337 2337
Size: 7054
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5665 136
Size: 107 122 71 631 605 3993 1525
Fragments arranged by size:
3993 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2507 7049 2507
Size: 4542 2512
BlpI GC'TnA_GC
Cuts at: 3729 4471 3729
Size: 742 6312
Bme1580I G_kGCm'C
Cuts at: 1790 2040 4226 5115 6271 1790
Size: 250 2186 889 1156 2573
Fragments arranged by size:
2573 2186 1156 889 250
BmgBI CAC'GTC
Cuts at: 1318 3878 1318
Size: 2560 4494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4785 5743 442
Size: 416 53 83 399 24 3368 958 1753
Fragments arranged by size:
3368 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2334 2334
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2448 2629 2801 5714 1239
Size: 854 355 181 172 2913 2579
Fragments arranged by size:
2913 2579 854 355 181 172
Bpu10I CC'TnA_GC
Cuts at: 2264 3351 4311 2264
Size: 1087 960 5007
Fragments arranged by size:
5007 1087 960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3543 5179 6044 6342 3543
Size: 1636 865 298 4255
Fragments arranged by size:
4255 1636 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3615 5717 882
Size: 617 2116 2102 2219
Fragments arranged by size:
2219 2116 2102 617
BsaAI yAC'GTr
Cuts at: 493 1204 4351 4845 493
Size: 711 3147 494 2702
Fragments arranged by size:
3147 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2217 7048 2217
Size: 4831 2223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3369 4861 5243 5878
Size: 53 83 186 936 1836 1492 382 635
Cuts at: 5878 275
Size: 1451
Fragments arranged by size:
1836 1492 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3696 5487 5885 6159 6306 6717 3696
Size: 1791 398 274 147 411 4033
Fragments arranged by size:
4033 1791 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 1178
Size: 30 165 30 1128 30 5671
Fragments arranged by size:
5671 1128 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2539 3908 4061 4097 1186
Size: 1353 1369 153 36 4143
Fragments arranged by size:
4143 1369 1353 153 36
BseYI C'CCAG_C
Cuts at: 2543 3968 6257 6753 2543
Size: 1425 2289 496 2844
Fragments arranged by size:
2844 2289 1425 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6481
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4504 5265 6293 1540
Size: 275 186 2503 761 1028 2301
Fragments arranged by size:
2503 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2879 4226 5115 5200 6271 2142
Size: 737 1347 889 85 1071 2925
Fragments arranged by size:
2925 1347 1071 889 737 85
BsmI GAATG_Cn'
Cuts at: 2339 3066 4565 4658 2339
Size: 727 1499 93 4735
Fragments arranged by size:
4735 1499 727 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2610 2610
Size: 7054
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2142 2879 4226 4373 5115 5200
Size: 399 250 102 737 1347 147 742 85
Cuts at: 5200 6271 1391
Size: 1071 2174
Fragments arranged by size:
2174 1347 1071 742 737 399 250 147
102 85
BspEI T'CCGG_A
Cuts at: 3696 6717 3696
Size: 3021 4033
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3280 4409 844
Size: 2436 1129 3489
Fragments arranged by size:
3489 2436 1129
BsrBI CCG'CTC
Cuts at: 1394 1823 4941 1394
Size: 429 3118 3507
Fragments arranged by size:
3507 3118 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5549 5723 65
Size: 1183 4301 174 1396
Fragments arranged by size:
4301 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5698 5885 1700
Size: 110 3888 187 2869
Fragments arranged by size:
3888 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3865 96
Size: 3769 3285
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 5114 6126 5114
Size: 1012 6042
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3651 794
Size: 2857 4197
BstBI TT'CG_AA
Cuts at: 2007 5858 2007
Size: 3851 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7054
BstYI r'GATC_y
Cuts at: 1972 2479 2507 3481 3539 4384 4751 5136 5153
Size: 507 28 974 58 845 367 385 17
Cuts at: 5153 6605 6691 6703 7049 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 974 845 507 385 367 346
86 58 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 3947 4284 3947
Size: 337 6717
BtgI C'CryG_G
Cuts at: 513 1065 2022 2031 4145 513
Size: 552 957 9 2114 3422
Fragments arranged by size:
3422 2114 957 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6315
Fragments arranged by size:
6315 683 56
BtsI GCAGTG_nn'
Cuts at: 3740 4570 5364 5384 3740
Size: 830 794 20 5410
Fragments arranged by size:
5410 830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2551 2586 529
Size: 35 1987 35 4997
Fragments arranged by size:
4997 1987 35 35
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6061 809
Size: 507 4745 1802
Fragments arranged by size:
4745 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2306 3358 4501
Size: 54 1425 50 275 18 476 1052 1143
Cuts at: 4501 5390 7019 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1143 1052 889 476 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4501 1537
Size: 275 2689 4090
Fragments arranged by size:
4090 2689 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 3947 4038 4984 1353
Size: 884 1710 91 946 3423
Fragments arranged by size:
3423 1710 946 884 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5641 6015 6161 387
Size: 1064 138 4052 374 146 1280
Fragments arranged by size:
4052 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2140 2140
Size: 7054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2448 2629 2801 3452 3798 5117
Size: 393 461 355 181 172 651 346 1319
Cuts at: 5117 5714 6501 6769 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1319 787 651 597 461 393 355
346 268 181 172
EcoO109I rG'GnC_Cy
Cuts at: 2248 3107 3972 2248
Size: 859 865 5330
Fragments arranged by size:
5330 865 859
EcoRI G'AATT_C
Cuts at: 4157 4363 4741 4157
Size: 206 378 6470
Fragments arranged by size:
6470 378 206
EcoRV GAT'ATC
Cuts at: 2468 2468
Size: 7054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4177 4177
Size: 7054
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 6201 1678
Size: 4523 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 2531 2563 3632
Size: 32 135 32 502 32 425 32 1069
Cuts at: 3632 3664 4093 4125 5695 5727 5769 5801 1373
Size: 32 429 32 1570 32 42 32 2626
Fragments arranged by size:
2626 1570 1069 502 429 425 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4398 4644 669
Size: 988 281 2460 246 3079
Fragments arranged by size:
3079 2460 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4244 748
Size: 3496 3558
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KpnI G_GTAC'C
Cuts at: 4379 4379
Size: 7054
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 6168 6352 1305
Size: 1138 3725 184 2007
Fragments arranged by size:
3725 2007 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3360 10
Size: 54 1425 343 1528 3704
Fragments arranged by size:
3704 1528 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2673 3576 3720 5012 5371 5530 518
Size: 648 1507 903 144 1292 359 159 2042
Fragments arranged by size:
2042 1507 1292 903 648 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2033 2099 3529 4168 4470 5147 6295 6540
Size: 427 66 1430 639 302 677 1148 245
Cuts at: 6540 1606
Size: 2120
Fragments arranged by size:
2120 1430 1148 677 639 427 302 245
66
NcoI C'CATG_G
Cuts at: 513 1065 2022 4145 513
Size: 552 957 2123 3422
Fragments arranged by size:
3422 2123 957 552
NdeI CA'TA_TG
Cuts at: 387 3091 387
Size: 2704 4350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NsiI A_TGCA'T
Cuts at: 2339 2339
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 3041 3061 3586 3683 4412 5957 1132
Size: 97 1812 20 525 97 729 1545 2229
Fragments arranged by size:
2229 1812 1545 729 525 97 97 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7054
PciI A'CATG_T
Cuts at: 3057 3582 5953 3057
Size: 525 2371 4158
Fragments arranged by size:
4158 2371 525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3158 3433 4195 1108
Size: 2050 275 762 3967
Fragments arranged by size:
3967 2050 762 275
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 6636 6668 5123
Size: 32 1481 32 5509
Fragments arranged by size:
5509 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 3107 3972 2248
Size: 859 865 5330
Fragments arranged by size:
5330 865 859
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PstI C_TGCA'G
Cuts at: 830 4406 830
Size: 3576 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 2099 3529 4168 2099
Size: 1430 639 4985
Fragments arranged by size:
4985 1430 639
RsrII CG'GwC_CG
Cuts at: 4015 4015
Size: 7054
SacI G_AGCT'C
Cuts at: 2142 2142
Size: 7054
SacII CC_GC'GG
Cuts at: 2034 2034
Size: 7054
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2237
Size: 7054
SbfI CC_TGCA'GG
Cuts at: 4406 4406
Size: 7054
ScaI AGT'ACT
Cuts at: 3814 5302 7014 3814
Size: 1488 1712 3854
Fragments arranged by size:
3854 1712 1488
SfcI C'TryA_G
Cuts at: 826 1045 2596 4402 5537 6218 6409 826
Size: 219 1551 1806 1135 681 191 1471
Fragments arranged by size:
1806 1551 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 4381 1872
Size: 2509 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3558 3618 5158 6059 6321 820
Size: 197 926 1615 60 1540 901 262 1553
Fragments arranged by size:
1615 1553 1540 926 901 262 197 60
SnaBI TAC'GTA
Cuts at: 493 4351 4845 493
Size: 3858 494 2702
Fragments arranged by size:
3858 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 3041 4412 1132
Size: 1909 1371 3774
Fragments arranged by size:
3774 1909 1371
SspI AAT'ATT
Cuts at: 5 52 2166 3025 3291 4978 5
Size: 47 2114 859 266 1687 2081
Fragments arranged by size:
2114 2081 1687 859 266 47
StuI AGG'CCT
Cuts at: 1517 3157 1517
Size: 1640 5414
StyI C'CwwG_G
Cuts at: 513 1065 2022 3152 3204 3931 4029 4145 4493
Size: 552 957 1130 52 727 98 116 348
Cuts at: 4493 513
Size: 3074
Fragments arranged by size:
3074 1130 957 727 552 348 116 98
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2518 5081 5098 5251 5436 5881 325
Size: 1255 938 2563 17 153 185 445 1498
Fragments arranged by size:
2563 1498 1255 938 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2312 3812 3865
Size: 274 80 33 51 1443 335 1500 53
Cuts at: 3865 5300 7012 96
Size: 1435 1712 138
Fragments arranged by size:
1712 1500 1443 1435 335 274 138 80
53 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7014 7046 7014
Size: 32 7022
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7054
XbaI T'CTAG_A
Cuts at: 3457 4390 3457
Size: 933 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4336 4336
Size: 7054
XhoI C'TCGA_G
Cuts at: 1943 3618 1943
Size: 1675 5379
XmaI C'CCGG_G
Cuts at: 1870 4379 1870
Size: 2509 4545
XmnI GAAnn'nnTTC
Cuts at: 3453 3465 5183 3453
Size: 12 1718 5324
Fragments arranged by size:
5324 1718 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3370 4862 276
Size: 53 83 186 936 1836 1492 2468
Fragments arranged by size:
2468 1836 1492 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI SanDI SexAI SfiI SfoI SrfI SwaI