Restriction map for
pFN21ASDA0323
(Circular) (Six-base) MAPSORT of: pFN21ASDA0323.seq Check: 1813 from: 1 to: 6886
pFN21ASDA0323 6886 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3238 3238
Size: 6886
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 3868 4207 3868
Size: 339 6547
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2233 2375 2541 3586 3762 3938 4949
Size: 419 182 142 166 1045 176 176 1011
Cuts at: 4949 6333 6601 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1045 1011 419 268 182 176
176 166 142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 5785 6762 1203
Size: 4582 977 1327
Fragments arranged by size:
4582 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AlwNI CAG_nnn'CTG
Cuts at: 4072 4629 6201 4072
Size: 557 1572 4757
Fragments arranged by size:
4757 1572 557
ApaI G_GGCC'C
Cuts at: 2164 2321 2164
Size: 157 6729
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 3911 4195 4421 4573 6682 1493
Size: 2418 284 226 152 2109 1697
Fragments arranged by size:
2418 2109 1697 284 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 2656 3159 3357 4041 4211 1870
Size: 73 713 503 198 684 170 4545
Fragments arranged by size:
4545 713 684 503 198 170 73
BamHI G'GATC_C
Cuts at: 3409 4216 4583 3409
Size: 807 367 5712
Fragments arranged by size:
5712 807 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2479 2488 3125 3214 3353
Size: 325 217 627 692 9 637 89 139
Cuts at: 3353 3868 4207 5662 618
Size: 515 339 1455 1842
Fragments arranged by size:
1842 1455 692 637 627 515 339 325
217 139 89 9
BanII G_rGCy'C
Cuts at: 1391 2164 2321 2431 2786 2824 4205 1391
Size: 773 157 110 355 38 1381 4072
Fragments arranged by size:
4072 1381 773 355 157 110 38
BbeI G_GCGC'C
Cuts at: 2492 3357 2492
Size: 865 6021
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3936 4043 928
Size: 971 2037 107 3771
Fragments arranged by size:
3771 2037 971 107
BbvCI CC'TCA_GC
Cuts at: 3656 3656
Size: 6886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5077 5111 1903
Size: 34 3140 34 3678
Fragments arranged by size:
3678 3140 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 2505 1548
Size: 957 5929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3595 5497 136
Size: 107 122 71 631 605 1923 1902 1525
Fragments arranged by size:
1923 1902 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6881 6881
Size: 6886
BlpI GC'TnA_GC
Cuts at: 2570 2785 3954 4303 2570
Size: 215 1169 349 5153
Fragments arranged by size:
5153 1169 349 215
Bme1580I G_kGCm'C
Cuts at: 1790 2164 2321 2484 3130 3219 4947 6103 1790
Size: 374 157 163 646 89 1728 1156 2573
Fragments arranged by size:
2573 1728 1156 646 374 163 157 89
BmgBI CAC'GTC
Cuts at: 1318 2466 3289 1318
Size: 1148 823 4915
Fragments arranged by size:
4915 1148 823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3893 4617 5575
Size: 416 53 83 399 24 2476 724 958
Cuts at: 5575 442
Size: 1753
Fragments arranged by size:
2476 1753 958 724 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3188
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2479 2640 3161 3488 3502 4094 5546
Size: 1094 146 161 521 327 14 592 1452
Cuts at: 5546 1239
Size: 2579
Fragments arranged by size:
2579 1452 1094 592 521 327 161 146
14
Bpu10I CC'TnA_GC
Cuts at: 3656 3656
Size: 6886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5011 5876 6174 5011
Size: 865 298 5723
Fragments arranged by size:
5723 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3252 3270 5549 882
Size: 617 1753 18 2279 2219
Fragments arranged by size:
2279 2219 1753 617 18
BsaAI yAC'GTr
Cuts at: 493 1204 4183 4677 493
Size: 711 2979 494 2702
Fragments arranged by size:
2979 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3815 6880 3815
Size: 3065 3821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2489 2735 3354 4693
Size: 53 83 186 936 956 246 619 1339
Cuts at: 4693 5075 5710 275
Size: 382 635 1451
Fragments arranged by size:
1451 1339 956 936 635 619 382 246
186 83 53
BsaWI w'CCGG_w
Cuts at: 2284 2600 3235 5319 5717 5991 6138 6549 2284
Size: 316 635 2084 398 274 147 411 2621
Fragments arranged by size:
2621 2084 635 411 398 316 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3500 3530 1178
Size: 30 165 30 2097 30 4534
Fragments arranged by size:
4534 2097 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2723 3089 3105 3108 3164 3177 3195 3697
Size: 1537 366 16 3 56 13 18 502
Cuts at: 3697 3809 1186
Size: 112 4263
Fragments arranged by size:
4263 1537 502 366 112 56 18 16
13 3
BseYI C'CCAG_C
Cuts at: 2131 2574 2794 3218 6089 6585 2131
Size: 443 220 424 2871 496 2432
Fragments arranged by size:
2871 2432 496 443 424 220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 2747 3216 3563 1163
Size: 573 657 354 469 347 4486
Fragments arranged by size:
4486 657 573 469 354 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2465 2761 2786 2803 2824 4947 5032 6103 2465
Size: 296 25 17 21 2123 85 1071 3248
Fragments arranged by size:
3248 2123 1071 296 85 25 21 17
BsmI GAATG_Cn'
Cuts at: 3202 4029 4397 4490 3202
Size: 827 368 93 5598
Fragments arranged by size:
5598 827 368 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3279 3279
Size: 6886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 2321 2431 2465 2484 2761 2786
Size: 399 374 157 110 34 19 277 25
Cuts at: 2786 2803 2824 3130 3219 4205 4947 5032 6103
Size: 17 21 306 89 986 742 85 1071
Cuts at: 6103 1391
Size: 2174
Fragments arranged by size:
2174 1071 986 742 399 374 306 277
157 110 89 85 34 25 21 19
17
BspEI T'CCGG_A
Cuts at: 2284 2600 6549 2284
Size: 316 3949 2621
Fragments arranged by size:
3949 2621 316
BspHI T'CATG_A
Cuts at: 4775 6505 4775
Size: 1730 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3238 4241 844
Size: 2394 1003 3489
Fragments arranged by size:
3489 2394 1003
BsrBI CCG'CTC
Cuts at: 1394 1823 2566 4054 4773 1394
Size: 429 743 1488 719 3507
Fragments arranged by size:
3507 1488 743 719 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3528 3534 5381 5555 65
Size: 1183 2280 6 1847 174 1396
Fragments arranged by size:
2280 1847 1396 1183 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 3681 4946 5958 3681
Size: 1265 1012 4609
Fragments arranged by size:
4609 1265 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6886
BstBI TT'CG_AA
Cuts at: 2007 5690 2007
Size: 3683 3203
BstEII G'GTnAC_C
Cuts at: 1184 3563 1184
Size: 2379 4507
BstXI CCAn_nnnn'nTGG
Cuts at: 2947 2947
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 2858 3409 3702 4216 4583 4968 4985 6437
Size: 886 551 293 514 367 385 17 1452
Cuts at: 6437 6523 6535 6881 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 886 551 514 385 367 346
293 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3005 3005
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 3130 3245 3489 3559 513
Size: 552 2065 115 244 70 3840
Fragments arranged by size:
3840 2065 552 244 115 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 4402 5196 5216 2410
Size: 1992 794 20 4080
Fragments arranged by size:
4080 1992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DraIII CAC_nnn'GTG
Cuts at: 2933 3423 4102 2933
Size: 490 679 5717
Fragments arranged by size:
5717 679 490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3578 3940 5893 809
Size: 507 2262 362 1953 1802
Fragments arranged by size:
2262 1953 1802 507 362
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3442 3486 4333
Size: 54 1425 50 275 18 1612 44 847
Cuts at: 4333 5222 6851 8
Size: 889 1629 43
Fragments arranged by size:
1629 1612 1425 889 847 275 54 50
44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 2973 3738 3866 4002 4816 1353
Size: 1194 426 765 128 136 814 3423
Fragments arranged by size:
3423 1194 814 765 426 136 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2794 3446 3806 5473 5847 5993
Size: 1064 138 1205 652 360 1667 374 146
Cuts at: 5993 387
Size: 1280
Fragments arranged by size:
1667 1280 1205 1064 652 374 360 146
138
EcoICRI GAG'CTC
Cuts at: 2784 2822 2784
Size: 38 6848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2233 2333 2375 2479 2541 2640
Size: 393 419 182 100 42 104 62 99
Cuts at: 2640 3161 3488 3502 3586 3762 3938 4094 4949
Size: 521 327 14 84 176 176 156 855
Cuts at: 4949 5546 6333 6601 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 855 787 597 521 419 393 327
268 182 176 176 156 104 100 99
84 62 42 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2273 3068 3142 3791 2273
Size: 795 74 649 5368
Fragments arranged by size:
5368 795 649 74
EcoO109I rG'GnC_Cy
Cuts at: 2160 2317 2318 2359 2867 3061 3335 3400 3784
Size: 157 1 41 508 194 274 65 384
Cuts at: 3784 4159 2160
Size: 375 4887
Fragments arranged by size:
4887 508 384 375 274 194 157 65
41 1
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 2492 3357 3601 6033 1678
Size: 814 865 244 2432 2531
Fragments arranged by size:
2531 2432 865 814 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 5527 5559 5601
Size: 32 135 32 1271 32 2652 32 42
Cuts at: 5601 5633 1373
Size: 32 2626
Fragments arranged by size:
2652 2626 1271 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4230 4476 669
Size: 988 281 2292 246 3079
Fragments arranged by size:
3079 2292 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6886
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KasI G'GCGC_C
Cuts at: 2488 3353 2488
Size: 865 6021
KpnI G_GTAC'C
Cuts at: 3872 4211 3872
Size: 339 6547
MfeI C'AATT_G
Cuts at: 4485 4485
Size: 6886
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6000 6184 1305
Size: 4695 184 2007
Fragments arranged by size:
4695 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3444 3488 10
Size: 54 1425 343 1612 44 3408
Fragments arranged by size:
3408 1612 1425 343 54 44
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 4844 5203 5362 518
Size: 648 2373 1305 359 159 2042
Fragments arranged by size:
2373 2042 1305 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 3181 4069 4120 4302 4979 6127 6372
Size: 705 870 888 51 182 677 1148 245
Cuts at: 6372 1606
Size: 2120
Fragments arranged by size:
2120 1148 888 870 705 677 245 182
51
NarI GG'CG_CC
Cuts at: 2489 3354 2489
Size: 865 6021
NcoI C'CATG_G
Cuts at: 513 1065 3130 3245 3489 513
Size: 552 2065 115 244 3910
Fragments arranged by size:
3910 2065 552 244 115
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 4244 5789 1132
Size: 97 3015 1545 2229
Fragments arranged by size:
3015 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2431 2484 2940 3064 1348
Size: 1083 53 456 124 5170
Fragments arranged by size:
5170 1083 456 124 53
PciI A'CATG_T
Cuts at: 5785 5785
Size: 6886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PfoI T'CCnGG_A
Cuts at: 2703 3780 2703
Size: 1077 5809
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2867 3784 2867
Size: 917 5969
PshAI GACnn'nnGTC
Cuts at: 1613 3561 1613
Size: 1948 4938
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PspOMI G'GGCC_C
Cuts at: 2160 2317 2160
Size: 157 6729
PstI C_TGCA'G
Cuts at: 830 2058 2310 2369 2382 2652 3408 4119 4238
Size: 1228 252 59 13 270 756 711 119
Cuts at: 4238 830
Size: 3478
Fragments arranged by size:
3478 1228 756 711 270 252 119 59
13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 2311 4069 2311
Size: 1758 5128
SacI G_AGCT'C
Cuts at: 2786 2824 2786
Size: 38 6848
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SanDI GG'GwC_CC
Cuts at: 2867 2867
Size: 6886
SapI GCTCTTCn'nnn_
Cuts at: 2547 2547
Size: 6886
SbfI CC_TGCA'GG
Cuts at: 3408 4238 3408
Size: 830 6056
ScaI AGT'ACT
Cuts at: 3548 5134 6846 3548
Size: 1586 1712 3588
Fragments arranged by size:
3588 1712 1586
SfcI C'TryA_G
Cuts at: 826 1045 2054 2306 2365 2378 2648 3404 4115
Size: 219 1009 252 59 13 270 756 711
Cuts at: 4115 4234 5369 6050 6241 826
Size: 119 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 756 711 681 270 252
219 191 119 59 13
SfoI GGC'GCC
Cuts at: 2490 3355 2490
Size: 865 6021
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 2658 4043 4213 1872
Size: 786 1385 170 4545
Fragments arranged by size:
4545 1385 786 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4990 5891 6153 820
Size: 197 926 3047 901 262 1553
Fragments arranged by size:
3047 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4183 4677 493
Size: 3690 494 2702
Fragments arranged by size:
3690 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2322 2343 2809 3130 3245 3489 4325
Size: 552 1257 21 466 321 115 244 836
Cuts at: 4325 513
Size: 3074
Fragments arranged by size:
3074 1257 836 552 466 321 244 115
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2786 3011 4913 4930 5083 5268 5713
Size: 1255 1206 225 1902 17 153 185 445
Cuts at: 5713 325
Size: 1498
Fragments arranged by size:
1902 1498 1255 1206 445 225 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2615 3546 5132
Size: 274 80 33 51 1443 638 931 1586
Cuts at: 5132 6844 96
Size: 1712 138
Fragments arranged by size:
1712 1586 1443 931 638 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2917 2942 2949 2969 2974 3001 3500 3532 6846
Size: 25 7 20 5 27 499 32 3314
Cuts at: 6846 6878 2917
Size: 32 2925
Fragments arranged by size:
3314 2925 499 32 32 27 25 20
7 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 2656 4041 4211 1870
Size: 786 1385 170 4545
Fragments arranged by size:
4545 1385 786 170
XmnI GAAnn'nnTTC
Cuts at: 5015 5015
Size: 6886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI
SrfI SwaI XcmI