Restriction map for
pFN21ASDA0316
(Circular) (Six-base) MAPSORT of: pFN21ASDA0316.seq Check: 1066 from: 1 to: 8695
pFN21ASDA0316 8695 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5549 5549
Size: 8695
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6505 278
Size: 53 83 186 936 4969 2468
Fragments arranged by size:
4969 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3682 6038 1937
Size: 1745 2356 4594
Fragments arranged by size:
4594 2356 1745
Acc65I G'GTAC_C
Cuts at: 3202 6016 3202
Size: 2814 5881
AclI AA'CG_TT
Cuts at: 1563 6822 7195 1563
Size: 5259 373 3063
Fragments arranged by size:
5259 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2017 2064 2373 3501 3959 5334 5481 5655
Size: 385 47 309 1128 458 1375 147 174
Cuts at: 5655 5787 6758 8142 8410 1632
Size: 132 971 1384 268 1917
Fragments arranged by size:
1917 1384 1375 1128 971 458 385 309
268 174 147 132 47
AfeI AGC'GCT
Cuts at: 3323 3323
Size: 8695
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8498
AflIII A'CryG_T
Cuts at: 1203 7594 8571 1203
Size: 6391 977 1327
Fragments arranged by size:
6391 1327 977
AgeI A'CCGG_T
Cuts at: 2401 7526 2401
Size: 5125 3570
AhdI GACnn_n'nnGTC
Cuts at: 7424 7424
Size: 8695
AleI CACnn'nnGTG
Cuts at: 1166 5723 1166
Size: 4557 4138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4556 5104 1184
Size: 3372 548 4775
Fragments arranged by size:
4775 3372 548
AlwNI CAG_nnn'CTG
Cuts at: 2448 3635 3850 5712 5897 6438 8010 2448
Size: 1187 215 1862 185 541 1572 3133
Fragments arranged by size:
3133 1862 1572 1187 541 215 185
ApaI G_GGCC'C
Cuts at: 3351 3351
Size: 8695
ApaLI G'TGCA_C
Cuts at: 6752 7908 6752
Size: 1156 7539
ApoI r'AATT_y
Cuts at: 1493 4159 5213 6004 6230 6382 8491 1493
Size: 2666 1054 791 226 152 2109 1697
Fragments arranged by size:
2666 2109 1697 1054 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2391 7249 160
Size: 872 1359 4858 1606
Fragments arranged by size:
4858 1606 1359 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8695
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2368 3860 4514 4741 6020 1870
Size: 73 155 270 1492 654 227 1279 4545
Fragments arranged by size:
4545 1492 1279 654 270 227 155 73
BamHI G'GATC_C
Cuts at: 6025 6392 6025
Size: 367 8328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3202 5134 6016 7471
Size: 325 217 627 335 1080 1932 882 1455
Cuts at: 7471 618
Size: 1842
Fragments arranged by size:
1932 1842 1455 1080 882 627 335 325
217
BanII G_rGCy'C
Cuts at: 1391 3351 4648 5422 5660 5818 6014 1391
Size: 1960 1297 774 238 158 196 4072
Fragments arranged by size:
4072 1960 1297 774 238 196 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 2070 3761 4722 5220 928
Size: 971 124 47 1691 961 498 4403
Fragments arranged by size:
4403 1691 971 961 498 124 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2040 2074 6886 6920 1903
Size: 34 103 34 4812 34 3678
Fragments arranged by size:
4812 3678 103 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 6587 7797 3545
Size: 3042 1210 4443
Fragments arranged by size:
4443 3042 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7306 136
Size: 107 122 71 631 605 5634 1525
Fragments arranged by size:
5634 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8690 8690
Size: 8695
BlpI GC'TnA_GC
Cuts at: 3851 3900 6112 3851
Size: 49 2212 6434
Fragments arranged by size:
6434 2212 49
Bme1580I G_kGCm'C
Cuts at: 1790 3351 4584 6756 7912 1790
Size: 1561 1233 2172 1156 2573
Fragments arranged by size:
2573 2172 1561 1233 1156
BmgBI CAC'GTC
Cuts at: 1318 3421 5482 1318
Size: 2103 2061 4531
Fragments arranged by size:
4531 2103 2061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6426 7384 442
Size: 416 53 83 399 24 5009 958 1753
Fragments arranged by size:
5009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8695
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 8695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2789 4088 4113 4371 5285 7355 1239
Size: 1550 1299 25 258 914 2070 2579
Fragments arranged by size:
2579 2070 1550 1299 914 258 25
Bpu10I CC'TnA_GC
Cuts at: 5249 5446 5249
Size: 197 8498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2134 3236 6820 7685 7983 2134
Size: 1102 3584 865 298 2846
Fragments arranged by size:
3584 2846 1102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7358 882
Size: 617 5859 2219
Fragments arranged by size:
5859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3478 5992 6486 493
Size: 711 2274 2514 494 2702
Fragments arranged by size:
2702 2514 2274 711 494
BsaBI GATnn'nnATC
Cuts at: 8689 8689
Size: 8695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6502 6884 7519 275
Size: 53 83 186 936 4969 382 635 1451
Fragments arranged by size:
4969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2401 7128 7526 7800 7947 8358 2401
Size: 4727 398 274 147 411 2738
Fragments arranged by size:
4727 2738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3957 3987 5021 5051 1178
Size: 30 165 30 2554 30 1034 30 4822
Fragments arranged by size:
4822 2554 1034 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2238 2456 2582 3461 3464 3978 4307 5210
Size: 1052 218 126 879 3 514 329 903
Cuts at: 5210 5213 5822 1186
Size: 3 609 4059
Fragments arranged by size:
4059 1052 903 879 609 514 329 218
126 3 3
BseYI C'CCAG_C
Cuts at: 2281 2849 4639 4901 7898 8394 2281
Size: 568 1790 262 2997 496 2582
Fragments arranged by size:
2997 2582 1790 568 496 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2397 3210 3329 5048 5074 5840 5888
Size: 573 661 813 119 1719 26 766 48
Cuts at: 5888 1163
Size: 3970
Fragments arranged by size:
3970 1719 813 766 661 573 119 48
26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6145 6906 7934 1540
Size: 275 186 4144 761 1028 2301
Fragments arranged by size:
4144 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3798 4648 5255 5422 5660 6756 6841 7912 3798
Size: 850 607 167 238 1096 85 1071 4581
Fragments arranged by size:
4581 1096 1071 850 607 238 167 85
BsmI GAATG_Cn'
Cuts at: 6206 6299 6206
Size: 93 8602
BsmBI CGTCTCn'nnnn_
Cuts at: 4636 4636
Size: 8695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3351 3798 4584 4648 5255 5422 5660
Size: 399 1561 447 786 64 607 167 238
Cuts at: 5660 5818 6014 6756 6841 7912 1391
Size: 158 196 742 85 1071 2174
Fragments arranged by size:
2174 1561 1071 786 742 607 447 399
238 196 167 158 85 64
BspEI T'CCGG_A
Cuts at: 8358 8358
Size: 8695
BspHI T'CATG_A
Cuts at: 2812 6584 8314 2812
Size: 3772 1730 3193
Fragments arranged by size:
3772 3193 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5549 6050 844
Size: 4705 501 3489
Fragments arranged by size:
4705 3489 501
BsrBI CCG'CTC
Cuts at: 1394 1823 2425 5737 6582 1394
Size: 429 602 3312 845 3507
Fragments arranged by size:
3507 3312 845 602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3039 4440 4753 7190 7364 65
Size: 1183 1791 1401 313 2437 174 1396
Fragments arranged by size:
2437 1791 1401 1396 1183 313 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2401 3558 3798 4324 7339 7526 1700
Size: 110 591 1157 240 526 3015 187 2869
Fragments arranged by size:
3015 2869 1157 591 526 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 3036 3807 96
Size: 2940 771 4984
Fragments arranged by size:
4984 2940 771
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8695
BssSI C'ACGA_G
Cuts at: 4301 5232 6755 7767 4301
Size: 931 1523 1012 5229
Fragments arranged by size:
5229 1523 1012 931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8695
BstBI TT'CG_AA
Cuts at: 2007 7499 2007
Size: 5492 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8695
BstXI CCAn_nnnn'nTGG
Cuts at: 3394 3532 5725 3394
Size: 138 2193 6364
Fragments arranged by size:
6364 2193 138
BstYI r'GATC_y
Cuts at: 1972 2899 2923 4120 4554 6025 6392 6777 6794
Size: 927 24 1197 434 1471 367 385 17
Cuts at: 6794 8246 8332 8344 8690 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1471 1452 1197 927 434 385 367
346 86 24 17 12
BstZ17I GTA'TAC
Cuts at: 3683 3683
Size: 8695
Bsu36I CC'TnA_GG
Cuts at: 5565 5565
Size: 8695
BtgI C'CryG_G
Cuts at: 513 1065 2022 4203 5724 5833 5882 513
Size: 552 957 2181 1521 109 49 3326
Fragments arranged by size:
3326 2181 1521 957 552 109 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4197 4368 487
Size: 683 56 2971 171 4814
Fragments arranged by size:
4814 2971 683 171 56
BtsI GCAGTG_nn'
Cuts at: 2311 2409 4102 5624 5707 5713 6211 7005 7025
Size: 98 1693 1522 83 6 498 794 20
Cuts at: 7025 2311
Size: 3981
Fragments arranged by size:
3981 1693 1522 794 498 98 83 20
6
ClaI AT'CG_AT
Cuts at: 1554 2433 2459 1554
Size: 879 26 7790
Fragments arranged by size:
7790 879 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3685 3720 529
Size: 35 3121 35 5504
Fragments arranged by size:
5504 3121 35 35
DraI TTT'AAA
Cuts at: 5999 5999
Size: 8695
DraIII CAC_nnn'GTG
Cuts at: 2342 3783 2342
Size: 1441 7254
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7702 809
Size: 507 6386 1802
Fragments arranged by size:
6386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 2584 3021
Size: 54 1425 50 275 18 257 497 437
Cuts at: 3021 3597 4326 4437 4788 4833 5115 5307 5607
Size: 576 729 111 351 45 282 192 300
Cuts at: 5607 6142 7031 8660 8
Size: 535 889 1629 43
Fragments arranged by size:
1629 1425 889 729 576 535 497 437
351 300 282 275 257 192 111 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6142 1537
Size: 275 4330 4090
Fragments arranged by size:
4330 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4184 4476 4765 4860 5674 6625 1353
Size: 2831 292 289 95 814 951 3423
Fragments arranged by size:
3423 2831 951 814 292 289 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7282 7656 7802 387
Size: 1064 138 5693 374 146 1280
Fragments arranged by size:
5693 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4646 5420 5658 4646
Size: 774 238 7683
Fragments arranged by size:
7683 774 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2064 2373 2789 3501 3959 4088
Size: 393 385 47 309 416 712 458 129
Cuts at: 4088 4113 4371 5285 5334 5481 5655 5787 6758
Size: 25 258 914 49 147 174 132 971
Cuts at: 6758 7355 8142 8410 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 971 914 787 712 597 458 416
393 385 309 268 258 174 147 132
129 49 47 25
EcoNI CCTnn'n_nnAGG
Cuts at: 4389 4389
Size: 8695
EcoO109I rG'GnC_Cy
Cuts at: 2272 3348 3632 3868 4055 4114 4297 2272
Size: 1076 284 236 187 59 183 6670
Fragments arranged by size:
6670 1076 284 236 187 183 59
EcoRI G'AATT_C
Cuts at: 6004 6382 6004
Size: 378 8317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 8695
FspI TGC'GCA
Cuts at: 7201 7201
Size: 8695
HaeII r_GCGC'y
Cuts at: 1678 3325 4593 7842 1678
Size: 1647 1268 3249 2531
Fragments arranged by size:
3249 2531 1647 1268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2412 2444 2815 2847 2854
Size: 32 135 32 840 32 371 32 7
Cuts at: 2854 2886 4358 4390 4643 4675 4718 4750 5021
Size: 32 1472 32 253 32 43 32 271
Cuts at: 5021 5053 5516 5548 7336 7368 7410 7442 1373
Size: 32 463 32 1788 32 42 32 2626
Fragments arranged by size:
2626 1788 1472 840 463 371 271 253
135 43 42 32 32 32 32 32
32 32 32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3424 5243 6039 6285 669
Size: 988 281 1486 1819 796 246 3079
Fragments arranged by size:
3079 1819 1486 988 796 281 246
HindIII A'AGCT_T
Cuts at: 748 2054 5558 748
Size: 1306 3504 3885
Fragments arranged by size:
3885 3504 1306
HpaI GTT'AAC
Cuts at: 6285 6285
Size: 8695
KpnI G_GTAC'C
Cuts at: 3206 6020 3206
Size: 2814 5881
MfeI C'AATT_G
Cuts at: 2904 6294 2904
Size: 3390 5305
MluI A'CGCG_T
Cuts at: 8571 8571
Size: 8695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 7809 7993 1305
Size: 1269 5235 184 2007
Fragments arranged by size:
5235 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3599 4439 4790 4835 5309
Size: 54 1425 343 1767 840 351 45 474
Cuts at: 5309 5609 10
Size: 300 3096
Fragments arranged by size:
3096 1767 1425 840 474 351 343 300
54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 5009 5723 6653 7012 7171 518
Size: 648 3843 714 930 359 159 2042
Fragments arranged by size:
3843 2042 930 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3850 3904 4205 4901 6111 6788 7936 8181
Size: 2244 54 301 696 1210 677 1148 245
Cuts at: 8181 1606
Size: 2120
Fragments arranged by size:
2244 2120 1210 1148 696 677 301 245
54
NaeI GCC'GGC
Cuts at: 4326 4326
Size: 8695
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7186
Fragments arranged by size:
7186 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8695
NgoMIV G'CCGG_C
Cuts at: 4324 4324
Size: 8695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8695
NotI GC'GGCC_GC
Cuts at: 6142 6142
Size: 8695
NspI r_CATG'y
Cuts at: 1132 1229 4828 6053 7598 1132
Size: 97 3599 1225 1545 2229
Fragments arranged by size:
3599 2229 1545 1225 97
PasI CC'CwG_GG
Cuts at: 1348 5749 5819 1348
Size: 4401 70 4224
Fragments arranged by size:
4401 4224 70
PciI A'CATG_T
Cuts at: 7594 7594
Size: 8695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4028 4153 1108
Size: 2920 125 5650
Fragments arranged by size:
5650 2920 125
PfoI T'CCnGG_A
Cuts at: 3575 3575
Size: 8695
PmeI GTTT'AAAC
Cuts at: 5999 5999
Size: 8695
PmlI CAC'GTG
Cuts at: 3478 3478
Size: 8695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6764 6796 8277 8309 6764
Size: 32 1481 32 7150
Fragments arranged by size:
7150 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 3632 4055 4297 2272
Size: 1360 423 242 6670
Fragments arranged by size:
6670 1360 423 242
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8695
PsiI TTA'TAA
Cuts at: 6265 6265
Size: 8695
PspOMI G'GGCC_C
Cuts at: 3347 3347
Size: 8695
PstI C_TGCA'G
Cuts at: 830 2690 5655 6047 830
Size: 1860 2965 392 3478
Fragments arranged by size:
3478 2965 1860 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8695
PvuII CAG'CTG
Cuts at: 3850 3850
Size: 8695
SacI G_AGCT'C
Cuts at: 4648 5422 5660 4648
Size: 774 238 7683
Fragments arranged by size:
7683 774 238
SacII CC_GC'GG
Cuts at: 4206 4206
Size: 8695
SalI G'TCGA_C
Cuts at: 1936 6037 1936
Size: 4101 4594
SanDI GG'GwC_CC
Cuts at: 2272 2272
Size: 8695
SapI GCTCTTCn'nnn_
Cuts at: 4184 4860 5674 4184
Size: 676 814 7205
Fragments arranged by size:
7205 814 676
SbfI CC_TGCA'GG
Cuts at: 6047 6047
Size: 8695
ScaI AGT'ACT
Cuts at: 6943 8655 6943
Size: 1712 6983
SexAI A'CCwGG_T
Cuts at: 2338 2338
Size: 8695
SfcI C'TryA_G
Cuts at: 826 1045 2686 3823 3886 5651 6043 7178 7859
Size: 219 1641 1137 63 1765 392 1135 681
Cuts at: 7859 8050 826
Size: 191 1471
Fragments arranged by size:
1765 1641 1471 1137 1135 681 392 219
191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8695
SmaI CCC'GGG
Cuts at: 1872 2370 3862 6022 1872
Size: 498 1492 2160 4545
Fragments arranged by size:
4545 2160 1492 498
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2113 3251 6799 7700 7962 820
Size: 197 926 170 1138 3548 901 262 1553
Fragments arranged by size:
3548 1553 1138 926 901 262 197 170
SnaBI TAC'GTA
Cuts at: 493 5992 6486 493
Size: 5499 494 2702
Fragments arranged by size:
5499 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8695
SphI G_CATG'C
Cuts at: 1132 6053 1132
Size: 4921 3774
SspI AAT'ATT
Cuts at: 5 52 6619 5
Size: 47 6567 2081
Fragments arranged by size:
6567 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8695
StyI C'CwwG_G
Cuts at: 513 1065 2022 3237 3661 3999 4027 5662 6134
Size: 552 957 1215 424 338 28 1635 472
Cuts at: 6134 513
Size: 3074
Fragments arranged by size:
3074 1635 1215 957 552 472 424 338
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2431 4889 5029 5874 6722 6739 6892
Size: 1255 851 2458 140 845 848 17 153
Cuts at: 6892 7077 7522 325
Size: 185 445 1498
Fragments arranged by size:
2458 1498 1255 851 848 845 445 185
153 140 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3036 3807 6941
Size: 274 80 33 51 1443 1059 771 3134
Cuts at: 6941 8653 96
Size: 1712 138
Fragments arranged by size:
3134 1712 1443 1059 771 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 3769 3801 3957 3989 8655 8687 2181
Size: 32 1556 32 156 32 4666 32 2189
Fragments arranged by size:
4666 2189 1556 156 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2074 5035 5454 1316
Size: 758 2961 419 4557
Fragments arranged by size:
4557 2961 758 419
XbaI T'CTAG_A
Cuts at: 4648 6031 4648
Size: 1383 7312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3388 3388
Size: 8695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8695
XmaI C'CCGG_G
Cuts at: 1870 2368 3860 6020 1870
Size: 498 1492 2160 4545
Fragments arranged by size:
4545 2160 1492 498
XmnI GAAnn'nnTTC
Cuts at: 4193 6824 4193
Size: 2631 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6503 276
Size: 53 83 186 936 4969 2468
Fragments arranged by size:
4969 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SfiI SfoI SrfI SwaI