Restriction map for
pFN21ASDA0315
(Circular) (Six-base) MAPSORT of: pFN21ASDA0315.seq Check: 279 from: 1 to: 10243
pFN21ASDA0315 10243 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2060 2209 2864 3135 4371 6985 2060
Size: 149 655 271 1236 2614 5318
Fragments arranged by size:
5318 2614 1236 655 271 149
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4653 6620 8053 278
Size: 53 83 186 936 3117 1967 1433 2468
Fragments arranged by size:
3117 2468 1967 1433 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5673 6066 7586 1937
Size: 3736 393 1520 4594
Fragments arranged by size:
4594 3736 1520 393
Acc65I G'GTAC_C
Cuts at: 7564 7564
Size: 10243
AclI AA'CG_TT
Cuts at: 1563 4573 8370 8743 1563
Size: 3010 3797 373 3063
Fragments arranged by size:
3797 3063 3010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2896 5029 8306 9690 9958 1632
Size: 1264 2133 3277 1384 268 1917
Fragments arranged by size:
3277 2133 1917 1384 1264 268
AfeI AGC'GCT
Cuts at: 5429 5429
Size: 10243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10046
AflIII A'CryG_T
Cuts at: 1203 5157 9142 10119 1203
Size: 3954 3985 977 1327
Fragments arranged by size:
3985 3954 1327 977
AgeI A'CCGG_T
Cuts at: 9074 9074
Size: 10243
AhdI GACnn_n'nnGTC
Cuts at: 6616 8972 6616
Size: 2356 7887
AleI CACnn'nnGTG
Cuts at: 1166 2767 3170 3203 5597 7385 1166
Size: 1601 403 33 2394 1788 4024
Fragments arranged by size:
4024 2394 1788 1601 403 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2785 3188 4154 5858 7253 7518 1184
Size: 1601 403 966 1704 1395 265 3909
Fragments arranged by size:
3909 1704 1601 1395 966 403 265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 10211
AlwNI CAG_nnn'CTG
Cuts at: 2320 3549 4030 4320 6598 6742 7986 9558 2320
Size: 1229 481 290 2278 144 1244 1572 3005
Fragments arranged by size:
3005 2278 1572 1244 1229 481 290 144
ApaI G_GGCC'C
Cuts at: 2044 2234 2943 3622 4463 6329 2044
Size: 190 709 679 841 1866 5958
Fragments arranged by size:
5958 1866 841 709 679 190
ApaLI G'TGCA_C
Cuts at: 2254 2978 5822 8300 9456 2254
Size: 724 2844 2478 1156 3041
Fragments arranged by size:
3041 2844 2478 1156 724
ApoI r'AATT_y
Cuts at: 1493 5777 7552 7778 7930 10039 1493
Size: 4284 1775 226 152 2109 1697
Fragments arranged by size:
4284 2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8797 160
Size: 872 7765 1606
Fragments arranged by size:
7765 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10243
AvaI C'yCGr_G
Cuts at: 1870 1943 3024 5364 5534 7356 7568 1870
Size: 73 1081 2340 170 1822 212 4545
Fragments arranged by size:
4545 2340 1822 1081 212 170 73
BamHI G'GATC_C
Cuts at: 3264 4443 7296 7456 7573 7940 3264
Size: 1179 2853 160 117 367 5567
Fragments arranged by size:
5567 2853 1179 367 160 117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2852 3020 3248 3293
Size: 325 217 627 285 780 168 228 45
Cuts at: 3293 4751 4772 5253 5369 5420 6789 6955 6970
Size: 1458 21 481 116 51 1369 166 15
Cuts at: 6970 7564 9019 618
Size: 594 1455 1842
Fragments arranged by size:
1842 1458 1455 1369 780 627 594 481
325 285 228 217 168 166 116 51
45 21 15
BanII G_rGCy'C
Cuts at: 1391 2044 2234 2498 2943 3095 3159 3622 4463
Size: 653 190 264 445 152 64 463 841
Cuts at: 4463 4793 5088 5475 6150 6282 6329 6584 7562
Size: 330 295 387 675 132 47 255 978
Cuts at: 7562 1391
Size: 4072
Fragments arranged by size:
4072 978 841 675 653 463 445 387
330 295 264 255 190 152 132 64
47
BbeI G_GCGC'C
Cuts at: 6959 6959
Size: 10243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2698 2707 2784 2902 4645 5176 5447
Size: 971 799 9 77 118 1743 531 271
Cuts at: 5447 5866 7047 7067 928
Size: 419 1181 20 4104
Fragments arranged by size:
4104 1743 1181 971 799 531 419 271
118 77 20 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 5377 5411 8434 8468 1903
Size: 34 2312 34 1094 34 3023 34 3678
Fragments arranged by size:
3678 3023 2312 1094 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8135 9345 8135
Size: 1210 9033
BclI T'GATC_A
Cuts at: 1548 5913 1548
Size: 4365 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2064 2873 3539
Size: 107 122 71 631 605 392 809 666
Cuts at: 3539 5500 5947 8854 136
Size: 1961 447 2907 1525
Fragments arranged by size:
2907 1961 1525 809 666 631 605 447
392 122 107 71
BglII A'GATC_T
Cuts at: 6879 7254 10238 6879
Size: 375 2984 6884
Fragments arranged by size:
6884 2984 375
BlpI GC'TnA_GC
Cuts at: 5615 7211 7660 5615
Size: 1596 449 8198
Fragments arranged by size:
8198 1596 449
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2234 2258 2943 2982 3251 3622 4003
Size: 254 190 24 685 39 269 371 381
Cuts at: 4003 4424 4463 4935 5256 5263 5372 5646 5826
Size: 421 39 472 321 7 109 274 180
Cuts at: 5826 6329 6914 6966 8304 9460 1790
Size: 503 585 52 1338 1156 2573
Fragments arranged by size:
2573 1338 1156 685 585 503 472 421
381 371 321 274 269 254 190 180
109 52 39 39 24 7
BmgBI CAC'GTC
Cuts at: 1318 3840 4804 5608 5821 5908 7126 1318
Size: 2522 964 804 213 87 1218 4435
Fragments arranged by size:
4435 2522 1218 964 804 213 87
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3445 4018 4673
Size: 416 53 83 399 24 2028 573 655
Cuts at: 4673 7974 8932 442
Size: 3301 958 1753
Fragments arranged by size:
3301 2028 1753 958 655 573 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3328 6704 3328
Size: 3376 6867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2822 3079 3384 3905 4844 4925 5704
Size: 992 591 257 305 521 939 81 779
Cuts at: 5704 5756 5765 6050 6128 6164 6173 6587 6788
Size: 52 9 285 78 36 9 414 201
Cuts at: 6788 7535 8903 1239
Size: 747 1368 2579
Fragments arranged by size:
2579 1368 992 939 779 747 591 521
414 305 285 257 201 81 78 52
36 9 9
Bpu10I CC'TnA_GC
Cuts at: 2396 3431 3662 5937 2396
Size: 1035 231 2275 6702
Fragments arranged by size:
6702 2275 1035 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3254 4127 6245 7085 8368 9233 9531 3254
Size: 873 2118 840 1283 865 298 3966
Fragments arranged by size:
3966 2118 1283 873 865 840 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3360 4915 5447 6715 8906 882
Size: 617 1861 1555 532 1268 2191 2219
Fragments arranged by size:
2219 2191 1861 1555 1268 617 532
BsaAI yAC'GTr
Cuts at: 493 1204 3790 4126 5158 5296 6163 7540 8034
Size: 711 2586 336 1032 138 867 1377 494
Cuts at: 8034 493
Size: 2702
Fragments arranged by size:
2702 2586 1377 1032 867 711 494 336
138
BsaBI GATnn'nnATC
Cuts at: 6239 10237 6239
Size: 3998 6245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4509 4622 4650 4824
Size: 53 83 186 936 2976 113 28 174
Cuts at: 4824 5331 6447 6574 6617 6956 7149 8050 8432
Size: 507 1116 127 43 339 193 901 382
Cuts at: 8432 9067 275
Size: 635 1451
Fragments arranged by size:
2976 1451 1116 936 901 635 507 382
339 193 186 174 127 113 83 53
43 28
BsaWI w'CCGG_w
Cuts at: 2589 7080 8676 9074 9348 9495 9906 2589
Size: 4491 1596 398 274 147 411 2926
Fragments arranged by size:
4491 2926 1596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 3662 3692 4286
Size: 30 165 30 1374 30 855 30 594
Cuts at: 4286 4316 4397 4427 4775 4805 5963 5993 6269
Size: 30 81 30 348 30 1158 30 276
Cuts at: 6269 6299 6467 6497 6620 6650 7355 7385 1178
Size: 30 168 30 123 30 705 30 4036
Fragments arranged by size:
4036 1374 1158 855 705 594 348 276
168 165 123 81 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3289 3551 3683 3800 3902 5222 5762 6785
Size: 2103 262 132 117 102 1320 540 1023
Cuts at: 6785 6788 7466 1186
Size: 3 678 3963
Fragments arranged by size:
3963 2103 1320 1023 678 540 262 132
117 102 3
BseYI C'CCAG_C
Cuts at: 2053 2062 3139 3594 4037 5572 5730 6382 6714
Size: 9 1077 455 443 1535 158 652 332
Cuts at: 6714 7226 9446 9942 2053
Size: 512 2220 496 2354
Fragments arranged by size:
2354 2220 1535 1077 652 512 496 455
443 332 158 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2791 3430 3440 3653 4106 4220 5416
Size: 573 1055 639 10 213 453 114 1196
Cuts at: 5416 5504 6080 6449 7174 7331 7494 1163
Size: 88 576 369 725 157 163 3912
Fragments arranged by size:
3912 1196 1055 725 639 576 573 453
369 213 163 157 114 88 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3204 3213 4549 5869 5951 6803
Size: 275 186 1203 9 1336 1320 82 852
Cuts at: 6803 6807 7693 8454 9482 1540
Size: 4 886 761 1028 2301
Fragments arranged by size:
2301 1336 1320 1203 1028 886 852 761
275 186 82 9 4
BsiHKAI G_wGCw'C
Cuts at: 2258 2498 2576 2982 3126 5826 6150 8304 8389
Size: 240 78 406 144 2700 324 2154 85
Cuts at: 8389 9460 2258
Size: 1071 3041
Fragments arranged by size:
3041 2700 2154 1071 406 324 240 144
85 78
BsmI GAATG_Cn'
Cuts at: 2182 7754 7847 2182
Size: 5572 93 4578
Fragments arranged by size:
5572 4578 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4217 4448 4613 4217
Size: 231 165 9847
Fragments arranged by size:
9847 231 165
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2234 2258 2498 2576 2943 2982
Size: 399 254 190 24 240 78 367 39
Cuts at: 2982 3095 3126 3159 3251 3622 4003 4424 4463
Size: 113 31 33 92 371 381 421 39
Cuts at: 4463 4793 4935 5088 5256 5263 5372 5475 5646
Size: 330 142 153 168 7 109 103 171
Cuts at: 5646 5826 6150 6282 6329 6584 6914 6966 7562
Size: 180 324 132 47 255 330 52 596
Cuts at: 7562 8304 8389 9460 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 596 421 399 381 371
367 330 330 324 255 254 240 190
180 171 168 153 142 132 113 109
103 92 85 78 52 47 39 39
33 31 24 7
BspEI T'CCGG_A
Cuts at: 9906 9906
Size: 10243
BspHI T'CATG_A
Cuts at: 8132 9862 8132
Size: 1730 8513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2060 2209 2820 2864 3135 3452 3903 3938
Size: 1216 149 611 44 271 317 451 35
Cuts at: 3938 3951 4371 5332 5538 5994 6186 6750 6985
Size: 13 420 961 206 456 192 564 235
Cuts at: 6985 7303 7598 844
Size: 318 295 3489
Fragments arranged by size:
3489 1216 961 611 564 456 451 420
318 317 295 271 235 206 192 149
44 35 13
BsrBI CCG'CTC
Cuts at: 1394 1823 5614 6859 7078 8130 1394
Size: 429 3791 1245 219 1052 3507
Fragments arranged by size:
3791 3507 1245 1052 429 219
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 2548 8738 8912 65
Size: 1183 1219 81 6190 174 1396
Fragments arranged by size:
6190 1396 1219 1183 174 81
BsrFI r'CCGG_y
Cuts at: 1700 1810 2645 3415 3612 4156 4544 5831 5918
Size: 110 835 770 197 544 388 1287 87
Cuts at: 5918 5950 6633 8887 9074 1700
Size: 32 683 2254 187 2869
Fragments arranged by size:
2869 2254 1287 835 770 683 544 388
197 187 110 87 32
BsrGI T'GTAC_A
Cuts at: 96 6285 96
Size: 6189 4054
BssHII G'CGCG_C
Cuts at: 1925 2389 1925
Size: 464 9779
BssSI C'ACGA_G
Cuts at: 3953 4064 5825 7136 7385 8303 9315 3953
Size: 111 1761 1311 249 918 1012 4881
Fragments arranged by size:
4881 1761 1311 1012 918 249 111
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3453 3573 3909 4320 4329 6742 6796 6970
Size: 2659 120 336 411 9 2413 54 174
Cuts at: 6970 7309 794
Size: 339 3728
Fragments arranged by size:
3728 2659 2413 411 339 336 174 120
54 9
BstBI TT'CG_AA
Cuts at: 2007 9047 2007
Size: 7040 3203
BstEII G'GTnAC_C
Cuts at: 1184 2513 4159 4810 1184
Size: 1329 1646 651 6617
Fragments arranged by size:
6617 1646 1329 651
BstXI CCAn_nnnn'nTGG
Cuts at: 3883 4045 5140 3883
Size: 162 1095 8986
Fragments arranged by size:
8986 1095 162
BstYI r'GATC_y
Cuts at: 1972 3264 4443 4533 6594 6879 7254 7296 7456
Size: 1292 1179 90 2061 285 375 42 160
Cuts at: 7456 7573 7940 8325 8342 9794 9880 9892 10238
Size: 117 367 385 17 1452 86 12 346
Cuts at: 10238 1972
Size: 1977
Fragments arranged by size:
2061 1977 1452 1292 1179 385 375 367
346 285 160 117 90 86 42 17
12
Bsu36I CC'TnA_GG
Cuts at: 2081 2149 3981 5205 2081
Size: 68 1832 1224 7119
Fragments arranged by size:
7119 1832 1224 68
BtgI C'CryG_G
Cuts at: 513 1065 2022 2226 2499 2871 3058 3527 4768
Size: 552 957 204 273 372 187 469 1241
Cuts at: 4768 5067 5108 5646 5942 6520 513
Size: 299 41 538 296 578 4236
Fragments arranged by size:
4236 1241 957 578 552 538 469 372
299 296 273 204 187 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5062 5402 5644 7150 487
Size: 683 56 3836 340 242 1506 3580
Fragments arranged by size:
3836 3580 1506 683 340 242 56
BtsI GCAGTG_nn'
Cuts at: 2027 2822 2938 4138 6914 7759 8553 8573 2027
Size: 795 116 1200 2776 845 794 20 3697
Fragments arranged by size:
3697 2776 1200 845 795 794 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10208
DraI TTT'AAA
Cuts at: 7547 7547
Size: 10243
DraIII CAC_nnn'GTG
Cuts at: 3453 6796 6970 7141 3453
Size: 3343 174 171 6555
Fragments arranged by size:
6555 3343 174 171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3992 5177 6560 7130 9250 809
Size: 507 2676 1185 1383 570 2120 1802
Fragments arranged by size:
2676 2120 1802 1383 1185 570 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2454 2475
Size: 54 1425 50 275 18 393 231 21
Cuts at: 2475 2862 3079 3201 3259 3579 3792 4309 4542
Size: 387 217 122 58 320 213 517 233
Cuts at: 4542 4546 5014 5948 6168 6800 7690 8579 10208
Size: 4 468 934 220 632 890 889 1629
Cuts at: 10208 8
Size: 43
Fragments arranged by size:
1629 1425 934 890 889 632 517 468
393 387 320 275 233 231 220 217
213 122 58 54 50 43 21 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 3201 4546 5948 6800 7690 1537
Size: 275 1389 1345 1402 852 890 4090
Fragments arranged by size:
4090 1402 1389 1345 890 852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2372 5684 6143 6299 8173 1353
Size: 1019 3312 459 156 1874 3423
Fragments arranged by size:
3423 3312 1874 1019 459 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2402 7478 8830 9204 9350 387
Size: 1064 138 813 5076 1352 374 146 1280
Fragments arranged by size:
5076 1352 1280 1064 813 374 146 138
EcoICRI GAG'CTC
Cuts at: 2496 6148 2496
Size: 3652 6591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2822 2896 3079 3384 3905 4844
Size: 393 599 591 74 183 305 521 939
Cuts at: 4844 4925 5029 5704 5756 5765 6050 6128 6164
Size: 81 104 675 52 9 285 78 36
Cuts at: 6164 6173 6587 6788 7535 8306 8903 9690 9958
Size: 9 414 201 747 771 597 787 268
Cuts at: 9958 1239
Size: 1524
Fragments arranged by size:
1524 939 787 771 747 675 599 597
591 521 414 393 305 285 268 201
183 104 81 78 74 52 36 9
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 3665 6698 6767 6770 2369
Size: 1296 3033 69 3 5842
Fragments arranged by size:
5842 3033 1296 69 3
EcoO109I rG'GnC_Cy
Cuts at: 2041 2085 2504 2824 2939 3469 3940 4034 4179
Size: 44 419 320 115 530 471 94 145
Cuts at: 4179 4396 4460 4923 5283 5740 5965 6325 6463
Size: 217 64 463 360 457 225 360 138
Cuts at: 6463 6739 2041
Size: 276 5545
Fragments arranged by size:
5545 530 471 463 457 419 360 360
320 276 225 217 145 138 115 94
64 44
EcoRI G'AATT_C
Cuts at: 5777 7552 7930 5777
Size: 1775 378 8090
Fragments arranged by size:
8090 1775 378
EcoRV GAT'ATC
Cuts at: 3067 3067
Size: 10243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 5200 6085 7009 2116
Size: 3084 885 924 5350
Fragments arranged by size:
5350 3084 924 885
FseI GG_CCGG'CC
Cuts at: 4548 4548
Size: 10243
FspI TGC'GCA
Cuts at: 6194 8749 6194
Size: 2555 7688
HaeII r_GCGC'y
Cuts at: 1678 2347 3594 5431 6095 6757 6959 9390 1678
Size: 669 1247 1837 664 662 202 2431 2531
Fragments arranged by size:
2531 2431 1837 1247 669 664 662 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 3281 3313 3813
Size: 32 135 32 560 32 1117 32 500
Cuts at: 3813 3845 4775 4807 5105 5137 6467 6499 6602
Size: 32 930 32 298 32 1330 32 103
Cuts at: 6602 6634 6812 6844 8884 8916 8958 8990 1373
Size: 32 178 32 2040 32 42 32 2626
Fragments arranged by size:
2626 2040 1330 1117 930 560 500 298
178 135 103 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 5347 6649 7587 7833 669
Size: 988 281 373 3036 1302 938 246 3079
Fragments arranged by size:
3079 3036 1302 988 938 373 281 246
HindIII A'AGCT_T
Cuts at: 748 4999 748
Size: 4251 5992
HpaI GTT'AAC
Cuts at: 7833 7833
Size: 10243
KasI G'GCGC_C
Cuts at: 6955 6955
Size: 10243
KpnI G_GTAC'C
Cuts at: 7568 7568
Size: 10243
MfeI C'AATT_G
Cuts at: 7842 7842
Size: 10243
MluI A'CGCG_T
Cuts at: 10119 10119
Size: 10243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 4723 6006 6252 6582 9357 9541 1305
Size: 1260 2158 1283 246 330 2775 184 2007
Fragments arranged by size:
2775 2158 2007 1283 1260 330 246 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 2456 2477 6170 10
Size: 54 1425 343 393 231 21 3693 4083
Fragments arranged by size:
4083 3693 1425 393 343 231 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 2767 3170 3203 5138 5597 5663
Size: 648 1401 200 403 33 1935 459 66
Cuts at: 5663 5840 6455 6656 6666 7385 8201 8560 8719
Size: 177 615 201 10 719 816 359 159
Cuts at: 8719 518
Size: 2042
Fragments arranged by size:
2042 1935 1401 816 719 648 615 459
403 359 201 200 177 159 66 33
10
MspA1I CmG'CkG
Cuts at: 1606 2191 2209 2317 2436 2843 2968 3139 3529
Size: 585 18 108 119 407 125 171 390
Cuts at: 3529 3637 3924 4017 5110 5632 5734 5766 5944
Size: 108 287 93 1093 522 102 32 178
Cuts at: 5944 6040 6625 7480 7508 7659 8336 9484 9729
Size: 96 585 855 28 151 677 1148 245
Cuts at: 9729 1606
Size: 2120
Fragments arranged by size:
2120 1148 1093 855 677 585 585 522
407 390 287 245 178 171 151 125
119 108 108 102 96 93 32 28
18
NaeI GCC'GGC
Cuts at: 2647 3614 4546 5833 2647
Size: 967 932 1287 7057
Fragments arranged by size:
7057 1287 967 932
NarI GG'CG_CC
Cuts at: 6956 6956
Size: 10243
NcoI C'CATG_G
Cuts at: 513 1065 2022 5067 5646 513
Size: 552 957 3045 579 5110
Fragments arranged by size:
5110 3045 957 579 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10243
NgoMIV G'CCGG_C
Cuts at: 2645 3612 4544 5831 2645
Size: 967 932 1287 7057
Fragments arranged by size:
7057 1287 967 932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10243
NotI GC'GGCC_GC
Cuts at: 7690 7690
Size: 10243
NruI TCG'CGA
Cuts at: 5562 5562
Size: 10243
NspI r_CATG'y
Cuts at: 1132 1229 2770 7133 7601 9146 1132
Size: 97 1541 4363 468 1545 2229
Fragments arranged by size:
4363 2229 1545 1541 468 97
PasI CC'CwG_GG
Cuts at: 1348 3622 4465 1348
Size: 2274 843 7126
Fragments arranged by size:
7126 2274 843
PciI A'CATG_T
Cuts at: 9142 9142
Size: 10243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3549 4030 6028 7141 1108
Size: 2441 481 1998 1113 4210
Fragments arranged by size:
4210 2441 1998 1113 481
PfoI T'CCnGG_A
Cuts at: 6141 6150 6564 6672 6765 7002 7032 6141
Size: 9 414 108 93 237 30 9352
Fragments arranged by size:
9352 414 237 108 93 30 9
PmeI GTTT'AAAC
Cuts at: 7547 7547
Size: 10243
PmlI CAC'GTG
Cuts at: 3790 4126 3790
Size: 336 9907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8312 8344 9825 9857 8312
Size: 32 1481 32 8698
Fragments arranged by size:
8698 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2504 2824 3469 3940 4034 4179 4923 6463 6739
Size: 320 645 471 94 145 744 1540 276
Cuts at: 6739 2504
Size: 6008
Fragments arranged by size:
6008 1540 744 645 471 320 276 145
94
PshAI GACnn'nnGTC
Cuts at: 1613 4017 1613
Size: 2404 7839
PsiI TTA'TAA
Cuts at: 7813 7813
Size: 10243
PspOMI G'GGCC_C
Cuts at: 2040 2230 2939 3618 4459 6325 2040
Size: 190 709 679 841 1866 5958
Fragments arranged by size:
5958 1866 841 709 679 190
PstI C_TGCA'G
Cuts at: 830 2034 2208 2817 3180 4325 5094 5130 5400
Size: 1204 174 609 363 1145 769 36 270
Cuts at: 5400 6921 6982 7497 7595 830
Size: 1521 61 515 98 3478
Fragments arranged by size:
3478 1521 1204 1145 769 609 515 363
270 174 98 61 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10243
PvuII CAG'CTG
Cuts at: 2191 2209 2317 3637 4017 5734 7480 2191
Size: 18 108 1320 380 1717 1746 4954
Fragments arranged by size:
4954 1746 1717 1320 380 108 18
SacI G_AGCT'C
Cuts at: 2498 6150 2498
Size: 3652 6591
SacII CC_GC'GG
Cuts at: 3530 5111 5945 3530
Size: 1581 834 7828
Fragments arranged by size:
7828 1581 834
SalI G'TCGA_C
Cuts at: 1936 7585 1936
Size: 5649 4594
SanDI GG'GwC_CC
Cuts at: 2824 4034 4923 2824
Size: 1210 889 8144
Fragments arranged by size:
8144 1210 889
SapI GCTCTTCn'nnn_
Cuts at: 5684 6143 6299 5684
Size: 459 156 9628
Fragments arranged by size:
9628 459 156
SbfI CC_TGCA'GG
Cuts at: 7595 7595
Size: 10243
ScaI AGT'ACT
Cuts at: 6113 6989 7424 8491 10203 6113
Size: 876 435 1067 1712 6153
Fragments arranged by size:
6153 1712 1067 876 435
SexAI A'CCwGG_T
Cuts at: 3351 6510 6792 3351
Size: 3159 282 6802
Fragments arranged by size:
6802 3159 282
SfcI C'TryA_G
Cuts at: 826 1045 2030 2204 2813 3176 4321 4351 5090
Size: 219 985 174 609 363 1145 30 739
Cuts at: 5090 5126 5396 6917 6978 7493 7591 8726 9407
Size: 36 270 1521 61 515 98 1135 681
Cuts at: 9407 9598 826
Size: 191 1471
Fragments arranged by size:
1521 1471 1145 1135 985 739 681 609
515 363 270 219 191 174 98 61
36 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3539 3539
Size: 10243
SfoI GGC'GCC
Cuts at: 6957 6957
Size: 10243
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10243
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10243
SmaI CCC'GGG
Cuts at: 1872 3026 5366 7358 7570 1872
Size: 1154 2340 1992 212 4545
Fragments arranged by size:
4545 2340 1992 1154 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3269 4142 6260 7100 8347 9248
Size: 197 926 1326 873 2118 840 1247 901
Cuts at: 9248 9510 820
Size: 262 1553
Fragments arranged by size:
2118 1553 1326 1247 926 901 873 840
262 197
SnaBI TAC'GTA
Cuts at: 493 7540 8034 493
Size: 7047 494 2702
Fragments arranged by size:
7047 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10243
SphI G_CATG'C
Cuts at: 1132 7601 1132
Size: 6469 3774
SspI AAT'ATT
Cuts at: 5 52 8167 5
Size: 47 8115 2081
Fragments arranged by size:
8115 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 2609 3189 5219 5411 1517
Size: 630 462 580 2030 192 6349
Fragments arranged by size:
6349 2030 630 580 462 192
StyI C'CwwG_G
Cuts at: 513 1065 2022 5067 5175 5646 5889 6060 6699
Size: 552 957 3045 108 471 243 171 639
Cuts at: 6699 7248 7446 7682 513
Size: 549 198 236 3074
Fragments arranged by size:
3074 3045 957 639 552 549 471 243
236 198 171 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 4457 4790 5408 5471 5557 6821
Size: 1255 1884 993 333 618 63 86 1264
Cuts at: 6821 7078 8270 8287 8440 8625 9070 325
Size: 257 1192 17 153 185 445 1498
Fragments arranged by size:
1884 1498 1264 1255 1192 993 618 445
333 257 185 153 86 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5463 6111 6285
Size: 274 80 33 51 1443 3486 648 174
Cuts at: 6285 6357 6987 7422 8489 10201 96
Size: 72 630 435 1067 1712 138
Fragments arranged by size:
3486 1712 1443 1067 648 630 435 274
174 138 80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2801 2833 4585 4617 4749 4781 6620 6652 7355
Size: 32 1752 32 132 32 1839 32 703
Cuts at: 7355 7387 10203 10235 2801
Size: 32 2816 32 2809
Fragments arranged by size:
2816 2809 1839 1752 703 132 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2306 5870 1316
Size: 990 3564 5689
Fragments arranged by size:
5689 3564 990
XbaI T'CTAG_A
Cuts at: 7579 7579
Size: 10243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2485 4868 5140 6518 2344
Size: 141 2383 272 1378 6069
Fragments arranged by size:
6069 2383 1378 272 141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10243
XmaI C'CCGG_G
Cuts at: 1870 3024 5364 7356 7568 1870
Size: 1154 2340 1992 212 4545
Fragments arranged by size:
4545 2340 1992 1154 212
XmnI GAAnn'nnTTC
Cuts at: 2447 5777 8372 2447
Size: 3330 2595 4318
Fragments arranged by size:
4318 3330 2595
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4651 6618 8051 276
Size: 53 83 186 936 3117 1967 1433 2468
Fragments arranged by size:
3117 2468 1967 1433 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I FspAI
NsiI PacI PspXI PsrI RsrII SrfI SwaI