Restriction map for
pFN21ASDA0313
(Circular) (Six-base) MAPSORT of: pFN21ASDA0313.seq Check: 6238 from: 1 to: 9226
pFN21ASDA0313 9226 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5774 7036 278
Size: 53 83 186 936 4238 1262 2468
Fragments arranged by size:
4238 2468 1262 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4686 6569 1937
Size: 2749 1883 4594
Fragments arranged by size:
4594 2749 1883
Acc65I G'GTAC_C
Cuts at: 2843 6547 2843
Size: 3704 5522
AclI AA'CG_TT
Cuts at: 1563 7353 7726 1563
Size: 5790 373 3063
Fragments arranged by size:
5790 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3312 3802 4148 4313 4388 4740 4985 5316
Size: 1680 490 346 165 75 352 245 331
Cuts at: 5316 5352 6302 7289 8673 8941 1632
Size: 36 950 987 1384 268 1917
Fragments arranged by size:
1917 1680 1384 987 950 490 352 346
331 268 245 165 75 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9029
AflIII A'CryG_T
Cuts at: 1203 8125 9102 1203
Size: 6922 977 1327
Fragments arranged by size:
6922 1327 977
AgeI A'CCGG_T
Cuts at: 8057 8057
Size: 9226
AhdI GACnn_n'nnGTC
Cuts at: 2539 2831 4872 7955 2539
Size: 292 2041 3083 3810
Fragments arranged by size:
3810 3083 2041 292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9226
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 9194
AlwNI CAG_nnn'CTG
Cuts at: 5131 5450 6969 8541 5131
Size: 319 1519 1572 5816
Fragments arranged by size:
5816 1572 1519 319
ApaLI G'TGCA_C
Cuts at: 7283 8439 7283
Size: 1156 8070
ApoI r'AATT_y
Cuts at: 1493 2405 3089 3375 4152 4207 4344 4429 4731
Size: 912 684 286 777 55 137 85 302
Cuts at: 4731 5280 5463 6246 6535 6761 6913 9022 1493
Size: 549 183 783 289 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 783 777 684 549 302
289 286 226 183 152 137 85 55
AseI AT'TA_AT
Cuts at: 160 1032 7780 160
Size: 872 6748 1606
Fragments arranged by size:
6748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
AvaI C'yCGr_G
Cuts at: 1870 1943 2989 3198 5988 6551 1870
Size: 73 1046 209 2790 563 4545
Fragments arranged by size:
4545 2790 1046 563 209 73
AvrII C'CTAG_G
Cuts at: 5572 5572
Size: 9226
BamHI G'GATC_C
Cuts at: 5872 6556 6923 5872
Size: 684 367 8175
Fragments arranged by size:
8175 684 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2843 3473 4471 5535 6547
Size: 325 217 627 1056 630 998 1064 1012
Cuts at: 6547 8002 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1064 1056 1012 998 630 627
325 217
BanII G_rGCy'C
Cuts at: 1391 6186 6219 6545 1391
Size: 4795 33 326 4072
Fragments arranged by size:
4795 4072 326 33
BbeI G_GCGC'C
Cuts at: 5539 5539
Size: 9226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2286 2304 2916 4027 4991 5997 6033
Size: 971 387 18 612 1111 964 1006 36
Cuts at: 6033 928
Size: 4121
Fragments arranged by size:
4121 1111 1006 971 964 612 387 36
18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7417 7451 1903
Size: 34 5480 34 3678
Fragments arranged by size:
5480 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7118 8328 7118
Size: 1210 8016
BclI T'GATC_A
Cuts at: 1548 3868 3891 5614 5926 1548
Size: 2320 23 1723 312 4848
Fragments arranged by size:
4848 2320 1723 312 23
BfrBI ATG'CAT
Cuts at: 2866 5351 2866
Size: 2485 6741
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4940 6385 7837
Size: 107 122 71 631 605 3268 1445 1452
Cuts at: 7837 136
Size: 1525
Fragments arranged by size:
3268 1525 1452 1445 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3532 4537 9221 3532
Size: 1005 4684 3537
Fragments arranged by size:
4684 3537 1005
BlpI GC'TnA_GC
Cuts at: 4169 6643 4169
Size: 2474 6752
Bme1580I G_kGCm'C
Cuts at: 1790 3478 5229 6276 7287 8443 1790
Size: 1688 1751 1047 1011 1156 2573
Fragments arranged by size:
2573 1751 1688 1156 1047 1011
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4628 5415 5977
Size: 416 53 83 399 24 3211 787 562
Cuts at: 5977 6028 6957 7915 442
Size: 51 929 958 1753
Fragments arranged by size:
3211 1753 958 929 787 562 416 399
83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2551 2095
Size: 456 8770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5073 7886 1239
Size: 3834 2813 2579
Fragments arranged by size:
3834 2813 2579
Bpu10I CC'TnA_GC
Cuts at: 3851 4128 4712 3851
Size: 277 584 8365
Fragments arranged by size:
8365 584 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3681 5764 7351 8216 8514 3681
Size: 2083 1587 865 298 4393
Fragments arranged by size:
4393 2083 1587 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3991 4824 5075 7889 882
Size: 617 2492 833 251 2814 2219
Fragments arranged by size:
2814 2492 2219 833 617 251
BsaAI yAC'GTr
Cuts at: 493 1204 6523 7017 493
Size: 711 5319 494 2702
Fragments arranged by size:
5319 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3650 4379 9220 3650
Size: 729 4841 3656
Fragments arranged by size:
4841 3656 729
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5536 5771 6002 7033
Size: 53 83 186 936 4003 235 231 1031
Cuts at: 7033 7415 8050 275
Size: 382 635 1451
Fragments arranged by size:
4003 1451 1031 936 635 382 235 231
186 83 53
BsaWI w'CCGG_w
Cuts at: 2373 7659 8057 8331 8478 8889 2373
Size: 5286 398 274 147 411 2710
Fragments arranged by size:
5286 2710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2179 2209 2760 2790 5401
Size: 30 165 30 776 30 551 30 2611
Cuts at: 5401 5431 6191 6221 1178
Size: 30 760 30 4183
Fragments arranged by size:
4183 2611 776 760 551 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5006 5361 6503 1186
Size: 3820 355 1142 3909
Fragments arranged by size:
3909 3820 1142 355
BseYI C'CCAG_C
Cuts at: 2041 2703 5142 5826 5997 6356 8429 8925 2041
Size: 662 2439 684 171 359 2073 496 2342
Fragments arranged by size:
2439 2342 2073 684 662 496 359 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4394 5507 1163
Size: 573 2658 1113 4882
Fragments arranged by size:
4882 2658 1113 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6676 7437 8465 1540
Size: 275 186 4675 761 1028 2301
Fragments arranged by size:
4675 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 7287 7372 8443 7287
Size: 85 1071 8070
Fragments arranged by size:
8070 1071 85
BsmI GAATG_Cn'
Cuts at: 2881 4427 5545 6737 6830 2881
Size: 1546 1118 1192 93 5277
Fragments arranged by size:
5277 1546 1192 1118 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3478 5229 6186 6219 6276 6545 7287
Size: 399 1688 1751 957 33 57 269 742
Cuts at: 7287 7372 8443 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1751 1688 1071 957 742 399 269
85 57 33
BspEI T'CCGG_A
Cuts at: 8889 8889
Size: 9226
BspHI T'CATG_A
Cuts at: 3888 7115 8845 3888
Size: 3227 1730 4269
Fragments arranged by size:
4269 3227 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5870 6195 6581 844
Size: 5026 325 386 3489
Fragments arranged by size:
5026 3489 386 325
BsrBI CCG'CTC
Cuts at: 1394 1823 7113 1394
Size: 429 5290 3507
Fragments arranged by size:
5290 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3075 3746 5039 6186 7721 7895 65
Size: 1183 1827 671 1293 1147 1535 174 1396
Fragments arranged by size:
1827 1535 1396 1293 1183 1147 671 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7870 8057 1700
Size: 110 6060 187 2869
Fragments arranged by size:
6060 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9226
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9226
BssSI C'ACGA_G
Cuts at: 7286 8298 7286
Size: 1012 8214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2419 794
Size: 1625 7601
BstBI TT'CG_AA
Cuts at: 2007 8030 2007
Size: 6023 3203
BstEII G'GTnAC_C
Cuts at: 1184 2134 1184
Size: 950 8276
BstXI CCAn_nnnn'nTGG
Cuts at: 3601 4570 3601
Size: 969 8257
BstYI r'GATC_y
Cuts at: 1972 2317 2558 2893 3061 3532 4537 4648 5236
Size: 345 241 335 168 471 1005 111 588
Cuts at: 5236 5872 6556 6923 7308 7325 8777 8863 8875
Size: 636 684 367 385 17 1452 86 12
Cuts at: 8875 9221 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1005 684 636 588 471 385
367 346 345 335 241 168 111 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2996 3131 3999 2996
Size: 135 868 8223
Fragments arranged by size:
8223 868 135
BtgI C'CryG_G
Cuts at: 513 1065 2050 2634 5758 513
Size: 552 985 584 3124 3981
Fragments arranged by size:
3981 3124 985 584 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3788 487
Size: 683 56 2562 5925
Fragments arranged by size:
5925 2562 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 2490 4406 5024 5519 6742 7536 7556 2274
Size: 216 1916 618 495 1223 794 20 3944
Fragments arranged by size:
3944 1916 1223 794 618 495 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2937 2972 3900 3935 529
Size: 35 2373 35 928 35 5820
Fragments arranged by size:
5820 2373 928 35 35 35
DraI TTT'AAA
Cuts at: 3433 4261 5274 6530 3433
Size: 828 1013 1256 6129
Fragments arranged by size:
6129 1256 1013 828
DraIII CAC_nnn'GTG
Cuts at: 3980 3980
Size: 9226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4694 8233 809
Size: 507 3378 3539 1802
Fragments arranged by size:
3539 3378 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4744 5755
Size: 54 1425 50 275 18 1504 1410 1011
Cuts at: 5755 6673 7562 9191 8
Size: 918 889 1629 43
Fragments arranged by size:
1629 1504 1425 1410 1011 918 889 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6673 1537
Size: 275 4861 4090
Fragments arranged by size:
4861 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 2754 2871 3453 4289 4364 5841 7156
Size: 1167 234 117 582 836 75 1477 1315
Cuts at: 7156 1353
Size: 3423
Fragments arranged by size:
3423 1477 1315 1167 836 582 234 117
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4822 5611 7813 8187 8333 387
Size: 1064 138 3233 789 2202 374 146 1280
Fragments arranged by size:
3233 2202 1280 1064 789 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3312 3802 4148 4313 4388 4740 4985
Size: 393 1680 490 346 165 75 352 245
Cuts at: 4985 5073 5316 5352 6302 7289 7886 8673 8941
Size: 88 243 36 950 987 597 787 268
Cuts at: 8941 1239
Size: 1524
Fragments arranged by size:
1680 1524 987 950 787 597 490 393
352 346 268 245 243 165 88 75
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2851 2357
Size: 494 8732
EcoO109I rG'GnC_Cy
Cuts at: 2362 5631 2362
Size: 3269 5957
EcoRI G'AATT_C
Cuts at: 6246 6535 6913 6246
Size: 289 378 8559
Fragments arranged by size:
8559 378 289
EcoRV GAT'ATC
Cuts at: 2206 2206
Size: 9226
FspI TGC'GCA
Cuts at: 7732 7732
Size: 9226
HaeII r_GCGC'y
Cuts at: 1678 5539 8373 1678
Size: 3861 2834 2531
Fragments arranged by size:
3861 2834 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2179 2186 2211 2297
Size: 32 135 32 582 25 7 25 86
Cuts at: 2297 2329 2525 2557 2817 2849 3237 3269 4132
Size: 32 196 32 260 32 388 32 863
Cuts at: 4132 4164 4858 4890 4996 5028 5327 5359 5717
Size: 32 694 32 106 32 299 32 358
Cuts at: 5717 5749 7867 7899 7941 7973 1373
Size: 32 2118 32 42 32 2626
Fragments arranged by size:
2626 2118 863 694 582 388 358 299
260 196 135 106 86 42 32 32
32 32 32 32 32 32 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 2922 3186 3262 6570 6816
Size: 988 281 921 63 264 76 3308 246
Cuts at: 6816 669
Size: 3079
Fragments arranged by size:
3308 3079 988 921 281 264 246 76
63
HindIII A'AGCT_T
Cuts at: 748 4919 5268 748
Size: 4171 349 4706
Fragments arranged by size:
4706 4171 349
HpaI GTT'AAC
Cuts at: 2859 3186 6816 2859
Size: 327 3630 5269
Fragments arranged by size:
5269 3630 327
KasI G'GCGC_C
Cuts at: 5535 5535
Size: 9226
KpnI G_GTAC'C
Cuts at: 2847 6551 2847
Size: 3704 5522
MfeI C'AATT_G
Cuts at: 2367 6825 2367
Size: 4458 4768
MluI A'CGCG_T
Cuts at: 9102 9102
Size: 9226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2921 6372 8340 8524 1305
Size: 1616 3451 1968 184 2007
Fragments arranged by size:
3451 2007 1968 1616 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3336 10
Size: 54 1425 343 1504 5900
Fragments arranged by size:
5900 1504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3045 6158 7184 7543 7702 518
Size: 648 1879 3113 1026 359 159 2042
Fragments arranged by size:
3113 2042 1879 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3358 4281 4366 6356 6642 7319 8467 8712
Size: 1752 923 85 1990 286 677 1148 245
Cuts at: 8712 1606
Size: 2120
Fragments arranged by size:
2120 1990 1752 1148 923 677 286 245
85
NarI GG'CG_CC
Cuts at: 5536 5536
Size: 9226
NcoI C'CATG_G
Cuts at: 513 1065 2050 2634 513
Size: 552 985 584 7105
Fragments arranged by size:
7105 985 584 552
NdeI CA'TA_TG
Cuts at: 387 5904 387
Size: 5517 3709
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9226
NotI GC'GGCC_GC
Cuts at: 6673 6673
Size: 9226
NsiI A_TGCA'T
Cuts at: 2868 5353 2868
Size: 2485 6741
NspI r_CATG'y
Cuts at: 1132 1229 2749 6584 8129 1132
Size: 97 1520 3835 1545 2229
Fragments arranged by size:
3835 2229 1545 1520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9226
PciI A'CATG_T
Cuts at: 8125 8125
Size: 9226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5249 1108
Size: 4141 5085
PmeI GTTT'AAAC
Cuts at: 6530 6530
Size: 9226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7295 7327 8808 8840 7295
Size: 32 1481 32 7681
Fragments arranged by size:
7681 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 5631 2362
Size: 3269 5957
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9226
PsiI TTA'TAA
Cuts at: 6796 6796
Size: 9226
PstI C_TGCA'G
Cuts at: 830 2092 5019 5881 6578 830
Size: 1262 2927 862 697 3478
Fragments arranged by size:
3478 2927 1262 862 697
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9226
PvuII CAG'CTG
Cuts at: 3358 4281 4366 6356 3358
Size: 923 85 1990 6228
Fragments arranged by size:
6228 1990 923 85
SalI G'TCGA_C
Cuts at: 1936 6568 1936
Size: 4632 4594
SapI GCTCTTCn'nnn_
Cuts at: 2520 2871 2520
Size: 351 8875
SbfI CC_TGCA'GG
Cuts at: 5881 6578 5881
Size: 697 8529
ScaI AGT'ACT
Cuts at: 6172 7474 9186 6172
Size: 1302 1712 6212
Fragments arranged by size:
6212 1712 1302
SfcI C'TryA_G
Cuts at: 826 1045 2088 2926 3540 3906 4414 5015 5183
Size: 219 1043 838 614 366 508 601 168
Cuts at: 5183 5670 5676 5877 6574 7709 8390 8581 826
Size: 487 6 201 697 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 838 697 681 614 601
508 487 366 219 201 191 168 6
SfoI GGC'GCC
Cuts at: 5537 5537
Size: 9226
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9226
SmaI CCC'GGG
Cuts at: 1872 6553 1872
Size: 4681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3696 5779 7330 8231 8493 820
Size: 197 926 1753 2083 1551 901 262 1553
Fragments arranged by size:
2083 1753 1553 1551 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6523 7017 493
Size: 6030 494 2702
Fragments arranged by size:
6030 2702 494
SpeI A'CTAG_T
Cuts at: 152 2174 152
Size: 2022 7204
SphI G_CATG'C
Cuts at: 1132 6584 1132
Size: 5452 3774
SspI AAT'ATT
Cuts at: 5 52 3971 7150 5
Size: 47 3919 3179 2081
Fragments arranged by size:
3919 3179 2081 47
StuI AGG'CCT
Cuts at: 1517 6406 1517
Size: 4889 4337
StyI C'CwwG_G
Cuts at: 513 1065 2050 2634 5099 5396 5572 6472 6665
Size: 552 985 584 2465 297 176 900 193
Cuts at: 6665 513
Size: 3074
Fragments arranged by size:
3074 2465 985 900 584 552 297 193
176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2142 3110 7253 7270 7423 7608 8053
Size: 1255 562 968 4143 17 153 185 445
Cuts at: 8053 325
Size: 1498
Fragments arranged by size:
4143 1498 1255 968 562 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4835 6170 7472
Size: 274 80 33 51 1443 2858 1335 1302
Cuts at: 7472 9184 96
Size: 1712 138
Fragments arranged by size:
2858 1712 1443 1335 1302 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 9186 9218 2022
Size: 32 7132 32 2030
Fragments arranged by size:
7132 2030 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2443 1316
Size: 1127 8099
XbaI T'CTAG_A
Cuts at: 6562 6562
Size: 9226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5428 5428
Size: 9226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9226
XmaI C'CCGG_G
Cuts at: 1870 6551 1870
Size: 4681 4545
XmnI GAAnn'nnTTC
Cuts at: 2913 3351 4218 4753 7355 2913
Size: 438 867 535 2602 4784
Fragments arranged by size:
4784 2602 867 535 438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5772 7034 276
Size: 53 83 186 936 4238 1262 2468
Fragments arranged by size:
4238 2468 1262 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbvCI BsiWI BsmBI
BstZ17I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI