Restriction map for
pFN21ASDA0290
(Circular) (Six-base) MAPSORT of: pFN21ASDA0290.seq Check: 9017 from: 1 to: 7396
pFN21ASDA0290 7396 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2704 2941 3301 3673 2704
Size: 237 360 372 6427
Fragments arranged by size:
6427 372 360 237
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5206 278
Size: 53 83 186 936 3670 2468
Fragments arranged by size:
3670 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4739 1937
Size: 2802 4594
Acc65I G'GTAC_C
Cuts at: 4717 4717
Size: 7396
AclI AA'CG_TT
Cuts at: 1563 2866 5523 5896 1563
Size: 1303 2657 373 3063
Fragments arranged by size:
3063 2657 1303 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3780 5459 6843 7111 1632
Size: 2148 1679 1384 268 1917
Fragments arranged by size:
2148 1917 1679 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7199
AflIII A'CryG_T
Cuts at: 1203 2969 3743 6295 7272 1203
Size: 1766 774 2552 977 1327
Fragments arranged by size:
2552 1766 1327 977 774
AgeI A'CCGG_T
Cuts at: 6227 6227
Size: 7396
AhdI GACnn_n'nnGTC
Cuts at: 3460 3851 6125 3460
Size: 391 2274 4731
Fragments arranged by size:
4731 2274 391
AleI CACnn'nnGTG
Cuts at: 1166 2699 4361 1166
Size: 1533 1662 4201
Fragments arranged by size:
4201 1662 1533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4305 4455 1184
Size: 3121 150 4125
Fragments arranged by size:
4125 3121 150
AlwNI CAG_nnn'CTG
Cuts at: 2422 3282 4393 5139 6711 2422
Size: 860 1111 746 1572 3107
Fragments arranged by size:
3107 1572 1111 860 746
ApaI G_GGCC'C
Cuts at: 2103 4548 2103
Size: 2445 4951
ApaLI G'TGCA_C
Cuts at: 3098 5453 6609 3098
Size: 2355 1156 3885
Fragments arranged by size:
3885 2355 1156
ApoI r'AATT_y
Cuts at: 1493 2726 4705 4931 5083 7192 1493
Size: 1233 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 1233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5950 160
Size: 872 4918 1606
Fragments arranged by size:
4918 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7396
AvaI C'yCGr_G
Cuts at: 1870 1943 2404 2577 3120 3140 3858 3969 4258
Size: 73 461 173 543 20 718 111 289
Cuts at: 4258 4721 1870
Size: 463 4545
Fragments arranged by size:
4545 718 543 463 461 289 173 111
73 20
BamHI G'GATC_C
Cuts at: 4726 5093 4726
Size: 367 7029
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3206 3472 3512 3541 3571
Size: 325 217 627 1419 266 40 29 30
Cuts at: 3571 4352 4717 6172 618
Size: 781 365 1455 1842
Fragments arranged by size:
1842 1455 1419 781 627 365 325 266
217 40 30 29
BanII G_rGCy'C
Cuts at: 1391 2103 2672 3039 3084 3127 3527 3779 3794
Size: 712 569 367 45 43 400 252 15
Cuts at: 3794 3872 3887 4548 4715 1391
Size: 78 15 661 167 4072
Fragments arranged by size:
4072 712 661 569 400 367 252 167
78 45 43 15 15
BbeI G_GCGC'C
Cuts at: 3545 3545
Size: 7396
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2233 928
Size: 971 334 6091
Fragments arranged by size:
6091 971 334
BbvCI CC'TCA_GC
Cuts at: 4049 4049
Size: 7396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5587 5621 1903
Size: 34 3650 34 3678
Fragments arranged by size:
3678 3650 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 5288 6498 3575
Size: 1713 1210 4473
Fragments arranged by size:
4473 1713 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3204 6007 136
Size: 107 122 71 631 605 1532 2803 1525
Fragments arranged by size:
2803 1532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7391 7391
Size: 7396
BlpI GC'TnA_GC
Cuts at: 4813 4813
Size: 7396
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2795 3102 3209 3574 4294 4368 4548
Size: 313 692 307 107 365 720 74 180
Cuts at: 4548 5457 6613 1790
Size: 909 1156 2573
Fragments arranged by size:
2573 1156 909 720 692 365 313 307
180 107 74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3797 3987 4261
Size: 416 53 83 399 24 2380 190 274
Cuts at: 4261 5127 6085 442
Size: 866 958 1753
Fragments arranged by size:
2380 1753 958 866 416 399 274 190
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 4331 4555 2179
Size: 2152 224 5020
Fragments arranged by size:
5020 2152 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2972 3111 3362 3382 3597 3650 4475 6056
Size: 1733 139 251 20 215 53 825 1581
Cuts at: 6056 1239
Size: 2579
Fragments arranged by size:
2579 1733 1581 825 251 215 139 53
20
Bpu10I CC'TnA_GC
Cuts at: 3161 3773 4049 3161
Size: 612 276 6508
Fragments arranged by size:
6508 612 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 5521 6386 6684 2327
Size: 3194 865 298 3039
Fragments arranged by size:
3194 3039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 3247 3642 4133 4330 6059 882
Size: 617 651 1097 395 491 197 1729 2219
Fragments arranged by size:
2219 1729 1097 651 617 491 395 197
BsaAI yAC'GTr
Cuts at: 493 1204 2701 3746 4693 5187 493
Size: 711 1497 1045 947 494 2702
Fragments arranged by size:
2702 1497 1045 947 711 494
BsaBI GATnn'nnATC
Cuts at: 7390 7390
Size: 7396
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2409 3542 5203 5585
Size: 53 83 186 936 876 1133 1661 382
Cuts at: 5585 6220 275
Size: 635 1451
Fragments arranged by size:
1661 1451 1133 936 876 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5829 6227 6501 6648 7059 5829
Size: 398 274 147 411 6166
Fragments arranged by size:
6166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2470 2500 3299 3329 3839
Size: 30 165 30 1067 30 799 30 510
Cuts at: 3839 3869 4235 4265 1178
Size: 30 366 30 4309
Fragments arranged by size:
4309 1067 799 510 366 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2242 2648 3320 3406 3409 3660 4472
Size: 982 74 406 672 86 3 251 812
Cuts at: 4472 4669 1186
Size: 197 3913
Fragments arranged by size:
3913 982 812 672 406 251 197 86
74 3
BseYI C'CCAG_C
Cuts at: 2665 3239 3530 3824 3956 4527 4547 4556 6599
Size: 574 291 294 132 571 20 9 2043
Cuts at: 6599 7095 2665
Size: 496 2966
Fragments arranged by size:
2966 2043 574 571 496 294 291 132
20 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 2723 3306 3645 4554 1163
Size: 573 678 309 583 339 909 4005
Fragments arranged by size:
4005 909 678 583 573 339 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3033 4846 5607 6635 1540
Size: 275 186 1032 1813 761 1028 2301
Fragments arranged by size:
2301 1813 1032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2381 3102 4086 5457 5542 6613 2381
Size: 721 984 1371 85 1071 3164
Fragments arranged by size:
3164 1371 1071 984 721 85
BsmI GAATG_Cn'
Cuts at: 4907 5000 4907
Size: 93 7303
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 3615 2254
Size: 1361 6035
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2381 2672 2795 3039 3084 3102
Size: 399 313 278 291 123 244 45 18
Cuts at: 3102 3127 3209 3527 3574 3779 3794 3872 3887
Size: 25 82 318 47 205 15 78 15
Cuts at: 3887 4086 4294 4368 4548 4715 5457 5542 6613
Size: 199 208 74 180 167 742 85 1071
Cuts at: 6613 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 318 313 291 278
244 208 205 199 180 167 123 85
82 78 74 47 45 25 18 15
15
BspEI T'CCGG_A
Cuts at: 7059 7059
Size: 7396
BspHI T'CATG_A
Cuts at: 5285 7015 5285
Size: 1730 5666
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2704 2941 3301 3673 4751 844
Size: 1542 318 237 360 372 1078 3489
Fragments arranged by size:
3489 1542 1078 372 360 318 237
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 2470 2917 4537 5283 1394
Size: 429 407 240 447 1620 746 3507
Fragments arranged by size:
3507 1620 746 447 429 407 240
BsrDI GCAATG_nn'
Cuts at: 65 1248 3757 5891 6065 65
Size: 1183 2509 2134 174 1396
Fragments arranged by size:
2509 2134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2907 4344 4435 6040 6227 1700
Size: 110 1097 1437 91 1605 187 2869
Fragments arranged by size:
2869 1605 1437 1097 187 110 91
BsrGI T'GTAC_A
Cuts at: 96 4063 96
Size: 3967 3429
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7396
BssSI C'ACGA_G
Cuts at: 5456 6468 5456
Size: 1012 6384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2599 2837 794
Size: 1805 238 5353
Fragments arranged by size:
5353 1805 238
BstBI TT'CG_AA
Cuts at: 2007 6200 2007
Size: 4193 3203
BstEII G'GTnAC_C
Cuts at: 1184 4631 1184
Size: 3447 3949
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 3495 3621 3964 4564 2119
Size: 1376 126 343 600 4951
Fragments arranged by size:
4951 1376 600 343 126
BstYI r'GATC_y
Cuts at: 1972 2302 4389 4726 5093 5478 5495 6947 7033
Size: 330 2087 337 367 385 17 1452 86
Cuts at: 7033 7045 7391 1972
Size: 12 346 1977
Fragments arranged by size:
2087 1977 1452 385 367 346 337 330
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2645 2645
Size: 7396
BtgI C'CryG_G
Cuts at: 513 1065 2211 2628 3171 3943 4356 4563 513
Size: 552 1146 417 543 772 413 207 3346
Fragments arranged by size:
3346 1146 772 552 543 417 413 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2186 2873 487
Size: 683 56 960 687 5010
Fragments arranged by size:
5010 960 687 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 3807 4209 4307 4912 5706 5726 2660
Size: 1147 402 98 605 794 20 4330
Fragments arranged by size:
4330 1147 794 605 402 98 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7361
DraI TTT'AAA
Cuts at: 4700 4700
Size: 7396
DraIII CAC_nnn'GTG
Cuts at: 2089 2686 2089
Size: 597 6799
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 6403 809
Size: 507 3281 1806 1802
Fragments arranged by size:
3281 1806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 3030 3639
Size: 54 1425 50 275 18 363 837 609
Cuts at: 3639 3946 4510 4843 5732 7361 8
Size: 307 564 333 889 1629 43
Fragments arranged by size:
1629 1425 889 837 609 564 363 333
307 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3030 4843 1537
Size: 275 1218 1813 4090
Fragments arranged by size:
4090 1813 1218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3072 3799 4646 5326 1353
Size: 1719 727 847 680 3423
Fragments arranged by size:
3423 1719 847 727 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 2528 5983 6357 6503 387
Size: 1064 138 549 390 3455 374 146 1280
Fragments arranged by size:
3455 1280 1064 549 390 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2972 3111 3362 3382 3597 3650 3780
Size: 393 1340 139 251 20 215 53 130
Cuts at: 3780 4475 5459 6056 6843 7111 1239
Size: 695 984 597 787 268 1524
Fragments arranged by size:
1524 1340 984 787 695 597 393 268
251 215 139 130 53 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2961 3198 4319 2961
Size: 237 1121 6038
Fragments arranged by size:
6038 1121 237
EcoO109I rG'GnC_Cy
Cuts at: 2074 2099 2205 2217 3499 3617 3688 3757 4029
Size: 25 106 12 1282 118 71 69 272
Cuts at: 4029 4262 4544 2074
Size: 233 282 4926
Fragments arranged by size:
4926 1282 282 272 233 118 106 71
69 25 12
EcoRI G'AATT_C
Cuts at: 4705 5083 4705
Size: 378 7018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3343 3343
Size: 7396
FspI TGC'GCA
Cuts at: 5902 5902
Size: 7396
HaeII r_GCGC'y
Cuts at: 1678 2270 2554 3545 4192 6543 1678
Size: 592 284 991 647 2351 2531
Fragments arranged by size:
2531 2351 991 647 592 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3299 3331 3446 3478 3837
Size: 32 135 32 1727 32 115 32 359
Cuts at: 3837 3869 6037 6069 6111 6143 1373
Size: 32 2168 32 42 32 2626
Fragments arranged by size:
2626 2168 1727 359 135 115 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4740 4986 669
Size: 988 281 2802 246 3079
Fragments arranged by size:
3079 2802 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7396
HpaI GTT'AAC
Cuts at: 4986 4986
Size: 7396
KasI G'GCGC_C
Cuts at: 3541 3541
Size: 7396
KpnI G_GTAC'C
Cuts at: 4721 4721
Size: 7396
MfeI C'AATT_G
Cuts at: 4995 4995
Size: 7396
MluI A'CGCG_T
Cuts at: 7272 7272
Size: 7396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3087 6510 6694 1305
Size: 1782 3423 184 2007
Fragments arranged by size:
3423 2007 1782 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 3948 10
Size: 54 1425 343 363 1753 3458
Fragments arranged by size:
3458 1753 1425 363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 4361 4670 5354 5713 5872 518
Size: 648 1533 1662 309 684 359 159 2042
Fragments arranged by size:
2042 1662 1533 684 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2939 3152 3173 3282 3534 3960 4026 4527
Size: 1333 213 21 109 252 426 66 501
Cuts at: 4527 4629 4812 5489 6637 6882 1606
Size: 102 183 677 1148 245 2120
Fragments arranged by size:
2120 1333 1148 677 501 426 252 245
213 183 109 102 66 21
NaeI GCC'GGC
Cuts at: 2909 4437 2909
Size: 1528 5868
NarI GG'CG_CC
Cuts at: 3542 3542
Size: 7396
NcoI C'CATG_G
Cuts at: 513 1065 2211 2628 513
Size: 552 1146 417 5281
Fragments arranged by size:
5281 1146 552 417
NdeI CA'TA_TG
Cuts at: 387 2674 387
Size: 2287 5109
NgoMIV G'CCGG_C
Cuts at: 2907 4435 2907
Size: 1528 5868
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7396
NotI GC'GGCC_GC
Cuts at: 4843 4843
Size: 7396
NspI r_CATG'y
Cuts at: 1132 1229 2973 4642 4754 6299 1132
Size: 97 1744 1669 112 1545 2229
Fragments arranged by size:
2229 1744 1669 1545 112 97
PasI CC'CwG_GG
Cuts at: 1348 3200 3621 1348
Size: 1852 421 5123
Fragments arranged by size:
5123 1852 421
PciI A'CATG_T
Cuts at: 2969 6295 2969
Size: 3326 4070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2629 1108
Size: 1521 5875
PfoI T'CCnGG_A
Cuts at: 2133 2896 2949 2133
Size: 763 53 6580
Fragments arranged by size:
6580 763 53
PmeI GTTT'AAAC
Cuts at: 4700 4700
Size: 7396
PmlI CAC'GTG
Cuts at: 2701 2701
Size: 7396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5465 5497 6978 7010 5465
Size: 32 1481 32 5851
Fragments arranged by size:
5851 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2205 2217 3499 3757 4029 4262 2074
Size: 131 12 1282 258 272 233 5208
Fragments arranged by size:
5208 1282 272 258 233 131 12
PshAI GACnn'nnGTC
Cuts at: 1613 3259 4421 1613
Size: 1646 1162 4588
Fragments arranged by size:
4588 1646 1162
PsiI TTA'TAA
Cuts at: 4966 4966
Size: 7396
PspOMI G'GGCC_C
Cuts at: 2099 4544 2099
Size: 2445 4951
PspXI vC'TCGA_Gb
Cuts at: 3969 3969
Size: 7396
PstI C_TGCA'G
Cuts at: 830 2853 2938 3137 4188 4284 4748 830
Size: 2023 85 199 1051 96 464 3478
Fragments arranged by size:
3478 2023 1051 464 199 96 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7396
PvuII CAG'CTG
Cuts at: 2939 3282 3534 3960 4527 2939
Size: 343 252 426 567 5808
Fragments arranged by size:
5808 567 426 343 252
SacII CC_GC'GG
Cuts at: 3174 3174
Size: 7396
SalI G'TCGA_C
Cuts at: 1936 4738 1936
Size: 2802 4594
SanDI GG'GwC_CC
Cuts at: 2205 3499 4029 4262 2205
Size: 1294 530 233 5339
Fragments arranged by size:
5339 1294 530 233
SapI GCTCTTCn'nnn_
Cuts at: 3072 3799 3072
Size: 727 6669
SbfI CC_TGCA'GG
Cuts at: 2853 4748 2853
Size: 1895 5501
ScaI AGT'ACT
Cuts at: 5644 7356 5644
Size: 1712 5684
SexAI A'CCwGG_T
Cuts at: 4677 4677
Size: 7396
SfcI C'TryA_G
Cuts at: 826 1045 2849 2934 2940 3133 3535 4184 4280
Size: 219 1804 85 6 193 402 649 96
Cuts at: 4280 4744 5879 6560 6751 826
Size: 464 1135 681 191 1471
Fragments arranged by size:
1804 1471 1135 681 649 464 402 219
193 191 96 85 6
SfoI GGC'GCC
Cuts at: 3543 3543
Size: 7396
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7396
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7396
SmaI CCC'GGG
Cuts at: 1872 3122 3860 4260 4723 1872
Size: 1250 738 400 463 4545
Fragments arranged by size:
4545 1250 738 463 400
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 3969 5500 6401 6663 820
Size: 197 926 399 1627 1531 901 262 1553
Fragments arranged by size:
1627 1553 1531 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 4693 5187 493
Size: 4200 494 2702
Fragments arranged by size:
4200 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7396
SphI G_CATG'C
Cuts at: 1132 4754 1132
Size: 3622 3774
SrfI GCCC'GGGC
Cuts at: 3122 3122
Size: 7396
SspI AAT'ATT
Cuts at: 5 52 5320 5
Size: 47 5268 2081
Fragments arranged by size:
5268 2081 47
StuI AGG'CCT
Cuts at: 1517 2885 3635 1517
Size: 1368 750 5278
Fragments arranged by size:
5278 1368 750
StyI C'CwwG_G
Cuts at: 513 1065 2112 2211 2382 2628 2880 3636 3813
Size: 552 1047 99 171 246 252 756 177
Cuts at: 3813 4835 513
Size: 1022 3074
Fragments arranged by size:
3074 1047 1022 756 552 252 246 177
171 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3249 3718 4190 4334 4570 5423 5440
Size: 1255 1669 469 472 144 236 853 17
Cuts at: 5440 5593 5778 6223 325
Size: 153 185 445 1498
Fragments arranged by size:
1669 1498 1255 853 472 469 445 236
185 153 144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4063 5642 7354
Size: 274 80 33 51 1443 2086 1579 1712
Cuts at: 7354 96
Size: 138
Fragments arranged by size:
2086 1712 1579 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3839 3871 4029 4061 7356 7388 3839
Size: 32 158 32 3295 32 3847
Fragments arranged by size:
3847 3295 158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7396
XbaI T'CTAG_A
Cuts at: 4732 4732
Size: 7396
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 3473 3688 3811 4301 4450 2116
Size: 1357 215 123 490 149 5062
Fragments arranged by size:
5062 1357 490 215 149 123
XhoI C'TCGA_G
Cuts at: 1943 3969 1943
Size: 2026 5370
XmaI C'CCGG_G
Cuts at: 1870 3120 3858 4258 4721 1870
Size: 1250 738 400 463 4545
Fragments arranged by size:
4545 1250 738 463 400
XmnI GAAnn'nnTTC
Cuts at: 5525 5525
Size: 7396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5204 276
Size: 53 83 186 936 3670 2468
Fragments arranged by size:
3670 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PsrI
RsrII SacI SfiI SwaI