Restriction map for
pFN21ASDA0289
(Circular) (Six-base) MAPSORT of: pFN21ASDA0289.seq Check: 1461 from: 1 to: 8611
pFN21ASDA0289 8611 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6421 278
Size: 53 83 186 936 4885 2468
Fragments arranged by size:
4885 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2546 5954 1937
Size: 609 3408 4594
Fragments arranged by size:
4594 3408 609
Acc65I G'GTAC_C
Cuts at: 4428 4635 5932 4428
Size: 207 1297 7107
Fragments arranged by size:
7107 1297 207
AclI AA'CG_TT
Cuts at: 1563 6738 7111 1563
Size: 5175 373 3063
Fragments arranged by size:
5175 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 3078 4396 5714 6674 8058 8326 1632
Size: 822 624 1318 1318 960 1384 268 1917
Fragments arranged by size:
1917 1384 1318 1318 960 822 624 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8414
AflIII A'CryG_T
Cuts at: 1203 5382 5846 7510 8487 1203
Size: 4179 464 1664 977 1327
Fragments arranged by size:
4179 1664 1327 977 464
AgeI A'CCGG_T
Cuts at: 7442 7442
Size: 8611
AhdI GACnn_n'nnGTC
Cuts at: 7340 7340
Size: 8611
AleI CACnn'nnGTG
Cuts at: 1166 3444 5135 1166
Size: 2278 1691 4642
Fragments arranged by size:
4642 2278 1691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3300 3668 1184
Size: 2116 368 6127
Fragments arranged by size:
6127 2116 368
AlwNI CAG_nnn'CTG
Cuts at: 2359 3339 4255 4306 5006 5863 6354 7926 2359
Size: 980 916 51 700 857 491 1572 3044
Fragments arranged by size:
3044 1572 980 916 857 700 491 51
ApaLI G'TGCA_C
Cuts at: 6668 7824 6668
Size: 1156 7455
ApoI r'AATT_y
Cuts at: 1493 3862 5633 5920 6146 6298 8407 1493
Size: 2369 1771 287 226 152 2109 1697
Fragments arranged by size:
2369 2109 1771 1697 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7165 160
Size: 872 6133 1606
Fragments arranged by size:
6133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8611
AvaI C'yCGr_G
Cuts at: 1870 1943 2245 5936 1870
Size: 73 302 3691 4545
Fragments arranged by size:
4545 3691 302 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5753 5786 5753
Size: 33 8578
BamHI G'GATC_C
Cuts at: 2104 2472 3112 3493 5941 6308 2104
Size: 368 640 381 2448 367 4407
Fragments arranged by size:
4407 2448 640 381 368 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2402 2708 4027 4428 4635
Size: 325 217 627 615 306 1319 401 207
Cuts at: 4635 4784 5317 5778 5932 7387 618
Size: 149 533 461 154 1455 1842
Fragments arranged by size:
1842 1455 1319 627 615 533 461 401
325 306 217 207 154 149
BanII G_rGCy'C
Cuts at: 1391 2040 2214 4374 4988 5124 5826 5930 1391
Size: 649 174 2160 614 136 702 104 4072
Fragments arranged by size:
4072 2160 702 649 614 174 136 104
BbeI G_GCGC'C
Cuts at: 4031 4031
Size: 8611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4686 5453 5849 928
Size: 971 2787 767 396 3690
Fragments arranged by size:
3690 2787 971 767 396
BbvCI CC'TCA_GC
Cuts at: 2468 2607 3659 4508 5705 2468
Size: 139 1052 849 1197 5374
Fragments arranged by size:
5374 1197 1052 849 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6802 6836 1903
Size: 34 4865 34 3678
Fragments arranged by size:
4865 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5879 6503 7713 5879
Size: 624 1210 6777
Fragments arranged by size:
6777 1210 624
BclI T'GATC_A
Cuts at: 1548 3186 5349 1548
Size: 1638 2163 4810
Fragments arranged by size:
4810 2163 1638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3692 7222 136
Size: 107 122 71 631 605 2020 3530 1525
Fragments arranged by size:
3530 2020 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2307 4362 5499 5901 8606 2307
Size: 2055 1137 402 2705 2312
Fragments arranged by size:
2705 2312 2055 1137 402
BlpI GC'TnA_GC
Cuts at: 4890 5258 5825 6028 4890
Size: 368 567 203 7473
Fragments arranged by size:
7473 567 368 203
Bme1580I G_kGCm'C
Cuts at: 1790 2407 2658 3415 3914 4338 6672 7828 1790
Size: 617 251 757 499 424 2334 1156 2573
Fragments arranged by size:
2573 2334 1156 757 617 499 424 251
BmgBI CAC'GTC
Cuts at: 1318 2101 3232 5437 5847 1318
Size: 783 1131 2205 410 4082
Fragments arranged by size:
4082 2205 1131 783 410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4407 5824
Size: 416 53 83 399 24 2051 939 1417
Cuts at: 5824 6342 7300 442
Size: 518 958 1753
Fragments arranged by size:
2051 1753 1417 958 939 518 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3619 4381 3619
Size: 762 7849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2294 2324 2365 2381 2686 2980 3332
Size: 902 153 30 41 16 305 294 352
Cuts at: 3332 3367 3815 4001 4361 4691 4824 4925 5028
Size: 35 448 186 360 330 133 101 103
Cuts at: 5028 5165 5304 5407 7271 1239
Size: 137 139 103 1864 2579
Fragments arranged by size:
2579 1864 902 448 360 352 330 305
294 186 153 139 137 133 103 103
101 41 35 30 16
Bpu10I CC'TnA_GC
Cuts at: 2180 2468 2607 2714 3273 3659 4508 4999 5705
Size: 288 139 107 559 386 849 491 706
Cuts at: 5705 2180
Size: 5086
Fragments arranged by size:
5086 849 706 559 491 386 288 139
107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5466 5750 6736 7601 7899 5466
Size: 284 986 865 298 6178
Fragments arranged by size:
6178 986 865 298 284
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 4725 5684 7274 882
Size: 617 2628 598 959 1590 2219
Fragments arranged by size:
2628 2219 1590 959 617 598
BsaAI yAC'GTr
Cuts at: 493 1204 5908 6402 493
Size: 711 4704 494 2702
Fragments arranged by size:
4704 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3191 8605 3191
Size: 5414 3197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4028 6418 6800 7435
Size: 53 83 186 936 2495 2390 382 635
Cuts at: 7435 275
Size: 1451
Fragments arranged by size:
2495 2390 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4455 7044 7442 7716 7863 8274 4455
Size: 2589 398 274 147 411 4792
Fragments arranged by size:
4792 2589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 2241 2271 3121
Size: 30 165 30 705 30 103 30 850
Cuts at: 3121 3151 3632 3662 1178
Size: 30 481 30 6127
Fragments arranged by size:
6127 850 705 481 165 103 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2477 3214 3776 4373 4631 4733 5186
Size: 1024 267 737 562 597 258 102 453
Cuts at: 5186 5867 1186
Size: 681 3930
Fragments arranged by size:
3930 1024 737 681 597 562 453 267
258 102
BseYI C'CCAG_C
Cuts at: 2061 3093 5123 5691 7814 8310 2061
Size: 1032 2030 568 2123 496 2362
Fragments arranged by size:
2362 2123 2030 1032 568 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2708 4569 5198 1163
Size: 573 972 1861 629 4576
Fragments arranged by size:
4576 1861 972 629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 6061 6822 7850 1540
Size: 275 186 90 3970 761 1028 2301
Fragments arranged by size:
3970 2301 1028 761 275 186 90
BsiHKAI G_wGCw'C
Cuts at: 3224 3279 4988 5000 5826 6672 6757 7828 3224
Size: 55 1709 12 826 846 85 1071 4007
Fragments arranged by size:
4007 1709 1071 846 826 85 55 12
BsmI GAATG_Cn'
Cuts at: 3470 3516 6122 6215 3470
Size: 46 2606 93 5866
Fragments arranged by size:
5866 2606 93 46
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2214 2407 2658 3224 3279 3415
Size: 399 250 174 193 251 566 55 136
Cuts at: 3415 3914 4338 4374 4988 5000 5124 5826 5930
Size: 499 424 36 614 12 124 702 104
Cuts at: 5930 6672 6757 7828 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 614 566 499 424
399 251 250 193 174 136 124 104
85 55 36 12
BspEI T'CCGG_A
Cuts at: 8274 8274
Size: 8611
BspHI T'CATG_A
Cuts at: 6500 8230 6500
Size: 1730 6881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4639 5966 844
Size: 3795 1327 3489
Fragments arranged by size:
3795 3489 1327
BsrBI CCG'CTC
Cuts at: 1394 1823 4723 6498 1394
Size: 429 2900 1775 3507
Fragments arranged by size:
3507 2900 1775 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2683 2863 4519 5310 5725 7106 7280
Size: 1183 1435 180 1656 791 415 1381 174
Cuts at: 7280 65
Size: 1396
Fragments arranged by size:
1656 1435 1396 1381 1183 791 415 180
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 2093 2553 3820 4894 7255 7442
Size: 110 258 25 460 1267 1074 2361 187
Cuts at: 7442 1700
Size: 2869
Fragments arranged by size:
2869 2361 1267 1074 460 258 187 110
25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8611
BssSI C'ACGA_G
Cuts at: 3215 4287 4545 5117 6671 7683 3215
Size: 1072 258 572 1554 1012 4143
Fragments arranged by size:
4143 1554 1072 1012 572 258
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4818 5715 794
Size: 4024 897 3690
Fragments arranged by size:
4024 3690 897
BstBI TT'CG_AA
Cuts at: 2007 7415 2007
Size: 5408 3203
BstEII G'GTnAC_C
Cuts at: 1184 4909 1184
Size: 3725 4886
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 4237 4795 2119
Size: 2118 558 5935
Fragments arranged by size:
5935 2118 558
BstYI r'GATC_y
Cuts at: 1972 2104 2307 2472 3112 3493 4362 5499 5566
Size: 132 203 165 640 381 869 1137 67
Cuts at: 5566 5901 5941 6308 6693 6710 8162 8248 8260
Size: 335 40 367 385 17 1452 86 12
Cuts at: 8260 8606 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1137 869 640 385 381 367
346 335 203 165 132 86 67 40
17 12
BstZ17I GTA'TAC
Cuts at: 2547 2547
Size: 8611
Bsu36I CC'TnA_GG
Cuts at: 4737 5837 4737
Size: 1100 7511
BtgI C'CryG_G
Cuts at: 513 1065 2022 2085 3399 3548 5109 5460 513
Size: 552 957 63 1314 149 1561 351 3664
Fragments arranged by size:
3664 1561 1314 957 552 351 149 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5659 487
Size: 683 56 4433 3439
Fragments arranged by size:
4433 3439 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2360 5043 6127 6921 6941 2153
Size: 207 2683 1084 794 20 3823
Fragments arranged by size:
3823 2683 1084 794 207 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2686 2721 5032 5067 529
Size: 35 2122 35 2311 35 4073
Fragments arranged by size:
4073 2311 2122 35 35 35
DraI TTT'AAA
Cuts at: 5915 5915
Size: 8611
DraIII CAC_nnn'GTG
Cuts at: 5394 5394
Size: 8611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7618 809
Size: 507 6302 1802
Fragments arranged by size:
6302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2088 3693
Size: 54 1425 50 275 18 252 6 1605
Cuts at: 3693 4326 4491 6058 6947 8576 8
Size: 633 165 1567 889 1629 43
Fragments arranged by size:
1629 1605 1567 1425 889 633 275 252
165 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2088 6058 1537
Size: 275 276 3970 4090
Fragments arranged by size:
4090 3970 276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2454 2588 3371 5516 6541 1353
Size: 875 226 134 783 2145 1025 3423
Fragments arranged by size:
3423 2145 1025 875 783 226 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3031 5834 7198 7572 7718 387
Size: 1064 138 1442 2803 1364 374 146 1280
Fragments arranged by size:
2803 1442 1364 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4986 5824 4986
Size: 838 7773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2294 2324 2365 2381 2454 2686
Size: 393 509 153 30 41 16 73 232
Cuts at: 2686 2980 3078 3332 3367 3815 4001 4361 4396
Size: 294 98 254 35 448 186 360 35
Cuts at: 4396 4691 4824 4925 5028 5165 5304 5407 5714
Size: 295 133 101 103 137 139 103 307
Cuts at: 5714 6674 7271 8058 8326 1239
Size: 960 597 787 268 1524
Fragments arranged by size:
1524 960 787 597 509 448 393 360
307 295 294 268 254 232 186 153
139 137 133 103 103 101 98 73
41 35 35 30 16
EcoO109I rG'GnC_Cy
Cuts at: 2578 4504 5593 5663 5701 5833 2578
Size: 1926 1089 70 38 132 5356
Fragments arranged by size:
5356 1926 1089 132 70 38
EcoRI G'AATT_C
Cuts at: 5633 5920 6298 5633
Size: 287 378 7946
Fragments arranged by size:
7946 378 287
EcoRV GAT'ATC
Cuts at: 2329 2329
Size: 8611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2996 3193 5764 2239
Size: 757 197 2571 5086
Fragments arranged by size:
5086 2571 757 197
FspI TGC'GCA
Cuts at: 2234 2675 7117 2234
Size: 441 4442 3728
Fragments arranged by size:
4442 3728 441
HaeII r_GCGC'y
Cuts at: 1678 2560 4031 4565 5422 7758 1678
Size: 882 1471 534 857 2336 2531
Fragments arranged by size:
2531 2336 1471 882 857 534
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 4097 4129 5001
Size: 32 135 32 669 32 1824 32 872
Cuts at: 5001 5033 5264 5296 5491 5523 7252 7284 7326
Size: 32 231 32 195 32 1729 32 42
Cuts at: 7326 7358 1373
Size: 32 2626
Fragments arranged by size:
2626 1824 1729 872 669 231 195 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3235 5955 6201 669
Size: 988 281 1297 2720 246 3079
Fragments arranged by size:
3079 2720 1297 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4094 5746 748
Size: 3346 1652 3613
Fragments arranged by size:
3613 3346 1652
HpaI GTT'AAC
Cuts at: 6201 6201
Size: 8611
KasI G'GCGC_C
Cuts at: 4027 4027
Size: 8611
KpnI G_GTAC'C
Cuts at: 4432 4639 5936 4432
Size: 207 1297 7107
Fragments arranged by size:
7107 1297 207
MfeI C'AATT_G
Cuts at: 6210 6210
Size: 8611
MluI A'CGCG_T
Cuts at: 8487 8487
Size: 8611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2985 4005 4548 7725 7909 1305
Size: 1680 1020 543 3177 184 2007
Fragments arranged by size:
3177 2007 1680 1020 543 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 3695 4328 4493 10
Size: 54 1425 343 252 1611 633 165 4128
Fragments arranged by size:
4128 1611 1425 633 343 252 165 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2397 3444 3731 3968 4064 5135
Size: 648 978 253 1047 287 237 96 1071
Cuts at: 5135 5381 6569 6928 7087 518
Size: 246 1188 359 159 2042
Fragments arranged by size:
2042 1188 1071 1047 978 648 359 287
253 246 237 159 96
MspA1I CmG'CkG
Cuts at: 1606 3097 3201 3325 3387 3870 4009 4657 6027
Size: 1491 104 124 62 483 139 648 1370
Cuts at: 6027 6704 7852 8097 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1491 1370 1148 677 648 483 245
139 124 104 62
NaeI GCC'GGC
Cuts at: 2070 2095 2555 2070
Size: 25 460 8126
Fragments arranged by size:
8126 460 25
NarI GG'CG_CC
Cuts at: 4028 4028
Size: 8611
NcoI C'CATG_G
Cuts at: 513 1065 2022 3399 3548 513
Size: 552 957 1377 149 5576
Fragments arranged by size:
5576 1377 957 552 149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8611
NgoMIV G'CCGG_C
Cuts at: 2068 2093 2553 2068
Size: 25 460 8126
Fragments arranged by size:
8126 460 25
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8611
NotI GC'GGCC_GC
Cuts at: 6058 6058
Size: 8611
NspI r_CATG'y
Cuts at: 1132 1229 3269 3649 5386 5969 7514 1132
Size: 97 2040 380 1737 583 1545 2229
Fragments arranged by size:
2229 2040 1737 1545 583 380 97
PasI CC'CwG_GG
Cuts at: 1348 3393 1348
Size: 2045 6566
PciI A'CATG_T
Cuts at: 5382 7510 5382
Size: 2128 6483
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3339 4024 4528 1108
Size: 2231 685 504 5191
Fragments arranged by size:
5191 2231 685 504
PfoI T'CCnGG_A
Cuts at: 2416 2721 2736 3423 4035 4593 5142 5562 2416
Size: 305 15 687 612 558 549 420 5465
Fragments arranged by size:
5465 687 612 558 549 420 305 15
PmeI GTTT'AAAC
Cuts at: 5915 5915
Size: 8611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6680 6712 8193 8225 6680
Size: 32 1481 32 7066
Fragments arranged by size:
7066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 5593 5663 5701 5833 2578
Size: 3015 70 38 132 5356
Fragments arranged by size:
5356 3015 132 70 38
PshAI GACnn'nnGTC
Cuts at: 1613 5028 1613
Size: 3415 5196
PsiI TTA'TAA
Cuts at: 6181 6181
Size: 8611
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3130 3162 3130
Size: 32 8579
PstI C_TGCA'G
Cuts at: 830 2784 2850 3836 3857 4443 5963 830
Size: 1954 66 986 21 586 1520 3478
Fragments arranged by size:
3478 1954 1520 986 586 66 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8611
PvuII CAG'CTG
Cuts at: 3097 3201 3325 4009 3097
Size: 104 124 684 7699
Fragments arranged by size:
7699 684 124 104
SacI G_AGCT'C
Cuts at: 4988 5826 4988
Size: 838 7773
SalI G'TCGA_C
Cuts at: 1936 5953 1936
Size: 4017 4594
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 8611
SbfI CC_TGCA'GG
Cuts at: 2850 5963 2850
Size: 3113 5498
ScaI AGT'ACT
Cuts at: 6859 8571 6859
Size: 1712 6899
SexAI A'CCwGG_T
Cuts at: 3582 3582
Size: 8611
SfcI C'TryA_G
Cuts at: 826 1045 2780 2846 3832 3853 4439 5785 5959
Size: 219 1735 66 986 21 586 1346 174
Cuts at: 5959 7094 7775 7966 826
Size: 1135 681 191 1471
Fragments arranged by size:
1735 1471 1346 1135 986 681 586 219
191 174 66 21
SfoI GGC'GCC
Cuts at: 4029 4029
Size: 8611
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8611
SgrAI Cr'CCGG_yG
Cuts at: 1700 2093 2553 1700
Size: 393 460 7758
Fragments arranged by size:
7758 460 393
SmaI CCC'GGG
Cuts at: 1872 2247 5938 1872
Size: 375 3691 4545
Fragments arranged by size:
4545 3691 375
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5481 5765 6715 7616 7878 820
Size: 197 926 3538 284 950 901 262 1553
Fragments arranged by size:
3538 1553 950 926 901 284 262 197
SnaBI TAC'GTA
Cuts at: 493 5908 6402 493
Size: 5415 494 2702
Fragments arranged by size:
5415 2702 494
SpeI A'CTAG_T
Cuts at: 152 4936 5525 152
Size: 4784 589 3238
Fragments arranged by size:
4784 3238 589
SphI G_CATG'C
Cuts at: 1132 5969 1132
Size: 4837 3774
SspI AAT'ATT
Cuts at: 5 52 2935 3564 3615 6535 5
Size: 47 2883 629 51 2920 2081
Fragments arranged by size:
2920 2883 2081 629 51 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8611
StyI C'CwwG_G
Cuts at: 513 1065 2022 2112 3399 3548 4230 6050 513
Size: 552 957 90 1287 149 682 1820 3074
Fragments arranged by size:
3074 1820 1287 957 682 552 149 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 4065 6638 6655 6808 6993 7438
Size: 1255 903 1582 2573 17 153 185 445
Cuts at: 7438 325
Size: 1498
Fragments arranged by size:
2573 1582 1498 1255 903 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3499 6857 8569
Size: 274 80 33 51 1443 1522 3358 1712
Cuts at: 8569 96
Size: 138
Fragments arranged by size:
3358 1712 1522 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 2145 2177 3632 3664 5833 5865 8571
Size: 32 5 32 1455 32 2169 32 2706
Cuts at: 8571 8603 2108
Size: 32 2116
Fragments arranged by size:
2706 2169 2116 1455 32 32 32 32
32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 3924 1316
Size: 2608 6003
XbaI T'CTAG_A
Cuts at: 5947 5947
Size: 8611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2702 2950 4193 4336 4529 2527
Size: 175 248 1243 143 193 6609
Fragments arranged by size:
6609 1243 248 193 175 143
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8611
XmaI C'CCGG_G
Cuts at: 1870 2245 5936 1870
Size: 375 3691 4545
Fragments arranged by size:
4545 3691 375
XmnI GAAnn'nnTTC
Cuts at: 6740 6740
Size: 8611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6419 276
Size: 53 83 186 936 4885 2468
Fragments arranged by size:
4885 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BsmBI EcoNI FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacII SapI SfiI SrfI SwaI