Restriction map for
pFN21ASDA0287
(Circular) (Six-base) MAPSORT of: pFN21ASDA0287.seq Check: 517 from: 1 to: 9121
pFN21ASDA0287 9121 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6354 6931 278
Size: 53 83 186 936 4818 577 2468
Fragments arranged by size:
4818 2468 936 577 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3954 4064 6464 1937
Size: 2017 110 2400 4594
Fragments arranged by size:
4594 2400 2017 110
Acc65I G'GTAC_C
Cuts at: 6442 6442
Size: 9121
AclI AA'CG_TT
Cuts at: 1563 3160 7248 7621 1563
Size: 1597 4088 373 3063
Fragments arranged by size:
4088 3063 1597 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2550 3293 4146 4741 5937 6066 6087
Size: 822 96 743 853 595 1196 129 21
Cuts at: 6087 7184 8568 8836 1632
Size: 1097 1384 268 1917
Fragments arranged by size:
1917 1384 1196 1097 853 822 743 595
268 129 96 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8924
AflIII A'CryG_T
Cuts at: 1203 8020 8997 1203
Size: 6817 977 1327
Fragments arranged by size:
6817 1327 977
AgeI A'CCGG_T
Cuts at: 2658 7952 2658
Size: 5294 3827
AhdI GACnn_n'nnGTC
Cuts at: 2185 4887 6379 7850 2185
Size: 2702 1492 1471 3456
Fragments arranged by size:
3456 2702 1492 1471
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9121
AlwNI CAG_nnn'CTG
Cuts at: 5747 6864 8436 5747
Size: 1117 1572 6432
Fragments arranged by size:
6432 1572 1117
ApaI G_GGCC'C
Cuts at: 4038 4038
Size: 9121
ApaLI G'TGCA_C
Cuts at: 7178 8334 7178
Size: 1156 7965
ApoI r'AATT_y
Cuts at: 1493 2600 3047 3332 3765 4412 6430 6656 6808
Size: 1107 447 285 433 647 2018 226 152
Cuts at: 6808 8917 1493
Size: 2109 1697
Fragments arranged by size:
2109 2018 1697 1107 647 447 433 285
226 152
AseI AT'TA_AT
Cuts at: 160 1032 7675 160
Size: 872 6643 1606
Fragments arranged by size:
6643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9121
AvaI C'yCGr_G
Cuts at: 1870 1943 4542 6446 1870
Size: 73 2599 1904 4545
Fragments arranged by size:
4545 2599 1904 73
BamHI G'GATC_C
Cuts at: 6451 6818 6451
Size: 367 8754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6314 6442 7897 618
Size: 325 217 627 4527 128 1455 1842
Fragments arranged by size:
4527 1842 1455 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2858 3750 4038 5574 5622 5946 6440 1391
Size: 1467 892 288 1536 48 324 494 4072
Fragments arranged by size:
4072 1536 1467 892 494 324 288 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 2442 4332 4788 4911 4941 6033
Size: 971 144 399 1890 456 123 30 1092
Cuts at: 6033 6120 928
Size: 87 3929
Fragments arranged by size:
3929 1890 1092 971 456 399 144 123
87 30
BbvCI CC'TCA_GC
Cuts at: 2352 2951 5427 2352
Size: 599 2476 6046
Fragments arranged by size:
6046 2476 599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7312 7346 1903
Size: 34 5375 34 3678
Fragments arranged by size:
5375 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7013 8223 7013
Size: 1210 7911
BclI T'GATC_A
Cuts at: 1548 6240 1548
Size: 4692 4429
BfrBI ATG'CAT
Cuts at: 2759 3070 2759
Size: 311 8810
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3209 7732 136
Size: 107 122 71 631 605 1537 4523 1525
Fragments arranged by size:
4523 1537 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9116 9116
Size: 9121
BlpI GC'TnA_GC
Cuts at: 3994 5449 5835 5877 5898 6538 3994
Size: 1455 386 42 21 640 6577
Fragments arranged by size:
6577 1455 640 386 42 21
Bme1580I G_kGCm'C
Cuts at: 1790 4038 7182 8338 1790
Size: 2248 3144 1156 2573
Fragments arranged by size:
3144 2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 2056 1318
Size: 738 8383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 4032 6852
Size: 416 53 83 399 24 732 1883 2820
Cuts at: 6852 7810 442
Size: 958 1753
Fragments arranged by size:
2820 1883 1753 958 732 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 4568 4654 3101
Size: 1467 86 7568
Fragments arranged by size:
7568 1467 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2275 4131 4398 5145 5429 7781 1239
Size: 1036 1856 267 747 284 2352 2579
Fragments arranged by size:
2579 2352 1856 1036 747 284 267
Bpu10I CC'TnA_GC
Cuts at: 2352 2942 2951 5427 6277 2352
Size: 590 9 2476 850 5196
Fragments arranged by size:
5196 2476 850 590 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4824 7246 8111 8409 4824
Size: 2422 865 298 5536
Fragments arranged by size:
5536 2422 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2172 3179 4487 4859 4874 7784 882
Size: 617 673 1007 1308 372 15 2910 2219
Fragments arranged by size:
2910 2219 1308 1007 673 617 372 15
BsaAI yAC'GTr
Cuts at: 493 1204 5551 6418 6912 493
Size: 711 4347 867 494 2702
Fragments arranged by size:
4347 2702 867 711 494
BsaBI GATnn'nnATC
Cuts at: 9115 9115
Size: 9121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 6351 6928 7310
Size: 53 83 186 936 2140 2678 577 382
Cuts at: 7310 7945 275
Size: 635 1451
Fragments arranged by size:
2678 2140 1451 936 635 577 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2145 2658 7554 7952 8226 8373 8784 2145
Size: 513 4896 398 274 147 411 2482
Fragments arranged by size:
4896 2482 513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 5269 5299 5573
Size: 30 165 30 870 30 2966 30 274
Cuts at: 5573 5603 1178
Size: 30 4696
Fragments arranged by size:
4696 2966 870 274 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4847 4877 4892 1186
Size: 3661 30 15 5415
Fragments arranged by size:
5415 3661 30 15
BseYI C'CCAG_C
Cuts at: 3479 3484 4424 5312 5530 8324 8820 3479
Size: 5 940 888 218 2794 496 3780
Fragments arranged by size:
3780 2794 940 888 496 218 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 3364 5523 6036 1163
Size: 573 708 920 2159 513 4248
Fragments arranged by size:
4248 2159 920 708 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 6571 7332 8360 1540
Size: 275 186 658 3912 761 1028 2301
Fragments arranged by size:
3912 2301 1028 761 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 3060 3393 3750 4611 6278 7182 7267 8338 3060
Size: 333 357 861 1667 904 85 1071 3843
Fragments arranged by size:
3843 1667 1071 904 861 357 333 85
BsmI GAATG_Cn'
Cuts at: 2761 5654 6182 6206 6632 6725 2761
Size: 2893 528 24 426 93 5157
Fragments arranged by size:
5157 2893 528 426 93 24
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2858 3060 3393 3750 4038 4611 5574
Size: 399 1068 202 333 357 288 573 963
Cuts at: 5574 5622 5946 6278 6440 7182 7267 8338 1391
Size: 48 324 332 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 963 742 573 399 357
333 332 324 288 202 162 85 48
BspEI T'CCGG_A
Cuts at: 8784 8784
Size: 9121
BspHI T'CATG_A
Cuts at: 4621 7010 8740 4621
Size: 2389 1730 5002
Fragments arranged by size:
5002 2389 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6008 6476 844
Size: 5164 468 3489
Fragments arranged by size:
5164 3489 468
BsrBI CCG'CTC
Cuts at: 1394 1823 7008 1394
Size: 429 5185 3507
Fragments arranged by size:
5185 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3003 4495 7616 7790 65
Size: 1183 1755 1492 3121 174 1396
Fragments arranged by size:
3121 1755 1492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2550 2658 7765 7952 1700
Size: 110 740 108 5107 187 2869
Fragments arranged by size:
5107 2869 740 187 110 108
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9121
BssSI C'ACGA_G
Cuts at: 2175 4455 7181 8193 2175
Size: 2280 2726 1012 3103
Fragments arranged by size:
3103 2726 2280 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5046 5540 5747 794
Size: 4252 494 207 4168
Fragments arranged by size:
4252 4168 494 207
BstBI TT'CG_AA
Cuts at: 2007 7925 2007
Size: 5918 3203
BstEII G'GTnAC_C
Cuts at: 1184 2129 1184
Size: 945 8176
BstYI r'GATC_y
Cuts at: 1972 2227 2477 4385 5002 6451 6818 7203 7220
Size: 255 250 1908 617 1449 367 385 17
Cuts at: 7220 8672 8758 8770 9116 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1908 1452 1449 617 385 367 346
255 250 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2222 2581 4885 5273 513
Size: 552 1157 359 2304 388 4361
Fragments arranged by size:
4361 2304 1157 552 388 359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8382
Fragments arranged by size:
8382 683 56
BtsI GCAGTG_nn'
Cuts at: 6637 7431 7451 6637
Size: 794 20 8307
Fragments arranged by size:
8307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5641 5676 529
Size: 35 5077 35 3974
Fragments arranged by size:
5077 3974 35 35
DraI TTT'AAA
Cuts at: 6425 6425
Size: 9121
DraIII CAC_nnn'GTG
Cuts at: 2587 3118 2587
Size: 531 8590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8128 809
Size: 507 6812 1802
Fragments arranged by size:
6812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 3718 5319
Size: 54 1425 50 275 18 613 1275 1601
Cuts at: 5319 6568 7457 9086 8
Size: 1249 889 1629 43
Fragments arranged by size:
1629 1601 1425 1275 1249 889 613 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6568 1537
Size: 275 4756 4090
Fragments arranged by size:
4756 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2083 2632 3842 5478 6042 6063 6371 7051
Size: 730 549 1210 1636 564 21 308 680
Cuts at: 7051 1353
Size: 3423
Fragments arranged by size:
3423 1636 1210 730 680 564 549 308
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7708 8082 8228 387
Size: 1064 138 6119 374 146 1280
Fragments arranged by size:
6119 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3748 3748
Size: 9121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2275 2454 2550 3293 4131 4146 4398
Size: 393 643 179 96 743 838 15 252
Cuts at: 4398 4741 5145 5429 5937 6066 6087 7184 7781
Size: 343 404 284 508 129 21 1097 597
Cuts at: 7781 8568 8836 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1097 838 787 743 643 597 508
404 393 343 284 268 252 179 129
96 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 4296 2351
Size: 1945 7176
EcoO109I rG'GnC_Cy
Cuts at: 4034 5586 4034
Size: 1552 7569
EcoRI G'AATT_C
Cuts at: 4412 6430 6808 4412
Size: 2018 378 6725
Fragments arranged by size:
6725 2018 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 3379 3802 2911
Size: 468 423 8230
Fragments arranged by size:
8230 468 423
FspI TGC'GCA
Cuts at: 7627 7627
Size: 9121
HaeII r_GCGC'y
Cuts at: 1678 2830 8268 1678
Size: 1152 5438 2531
Fragments arranged by size:
5438 2531 1152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2273 2294 2305
Size: 32 135 32 599 32 70 21 11
Cuts at: 2305 2309 2326 2341 2432 2464 2815 2847 2851
Size: 4 17 15 91 32 351 32 4
Cuts at: 2851 2883 3950 3982 4873 4905 5570 5602 6365
Size: 32 1067 32 891 32 665 32 763
Cuts at: 6365 6397 7762 7794 7836 7868 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 1067 891 763 665 599 351
135 91 70 42 32 32 32 32
32 32 32 32 32 32 32 32
21 17 15 11 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 5056 5527 6465 6711 669
Size: 988 281 3118 471 938 246 3079
Fragments arranged by size:
3118 3079 988 938 471 281 246
HindIII A'AGCT_T
Cuts at: 748 2257 748
Size: 1509 7612
HpaI GTT'AAC
Cuts at: 6711 6711
Size: 9121
KpnI G_GTAC'C
Cuts at: 6446 6446
Size: 9121
MfeI C'AATT_G
Cuts at: 2982 4899 6720 2982
Size: 1917 1821 5383
Fragments arranged by size:
5383 1917 1821
MluI A'CGCG_T
Cuts at: 8997 8997
Size: 9121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3130 8235 8419 1305
Size: 1825 5105 184 2007
Fragments arranged by size:
5105 2007 1825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2445 10
Size: 54 1425 343 613 6686
Fragments arranged by size:
6686 1425 613 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2930 3007 3441 5565 7079 7438 7597
Size: 648 1764 77 434 2124 1514 359 159
Cuts at: 7597 518
Size: 2042
Fragments arranged by size:
2124 2042 1764 1514 648 434 359 159
77
MspA1I CmG'CkG
Cuts at: 1606 2184 2224 2740 5134 5747 6537 7214 8362
Size: 578 40 516 2394 613 790 677 1148
Cuts at: 8362 8607 1606
Size: 245 2120
Fragments arranged by size:
2394 2120 1148 790 677 613 578 516
245 40
NcoI C'CATG_G
Cuts at: 513 1065 4885 513
Size: 552 3820 4749
Fragments arranged by size:
4749 3820 552
NdeI CA'TA_TG
Cuts at: 387 3004 3970 5379 387
Size: 2617 966 1409 4129
Fragments arranged by size:
4129 2617 1409 966
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9121
NotI GC'GGCC_GC
Cuts at: 6568 6568
Size: 9121
NsiI A_TGCA'T
Cuts at: 2761 3072 2761
Size: 311 8810
NspI r_CATG'y
Cuts at: 1132 1229 6479 8024 1132
Size: 97 5250 1545 2229
Fragments arranged by size:
5250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9121
PciI A'CATG_T
Cuts at: 8020 8020
Size: 9121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 3118 5153 1108
Size: 1230 780 2035 5076
Fragments arranged by size:
5076 2035 1230 780
PfoI T'CCnGG_A
Cuts at: 2379 4375 2379
Size: 1996 7125
PmeI GTTT'AAAC
Cuts at: 6425 6425
Size: 9121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7190 7222 8703 8735 7190
Size: 32 1481 32 7576
Fragments arranged by size:
7576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5586 5586
Size: 9121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9121
PsiI TTA'TAA
Cuts at: 6691 6691
Size: 9121
PspOMI G'GGCC_C
Cuts at: 4034 4034
Size: 9121
PspXI vC'TCGA_Gb
Cuts at: 4542 4542
Size: 9121
PstI C_TGCA'G
Cuts at: 830 5293 5752 5941 6473 830
Size: 4463 459 189 532 3478
Fragments arranged by size:
4463 3478 532 459 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9121
PvuII CAG'CTG
Cuts at: 5134 5747 5134
Size: 613 8508
SacI G_AGCT'C
Cuts at: 3750 3750
Size: 9121
SacII CC_GC'GG
Cuts at: 2225 2225
Size: 9121
SalI G'TCGA_C
Cuts at: 1936 6463 1936
Size: 4527 4594
SapI GCTCTTCn'nnn_
Cuts at: 2632 6042 6063 6371 2632
Size: 3410 21 308 5382
Fragments arranged by size:
5382 3410 308 21
SbfI CC_TGCA'GG
Cuts at: 6473 6473
Size: 9121
ScaI AGT'ACT
Cuts at: 6338 7369 9081 6338
Size: 1031 1712 6378
Fragments arranged by size:
6378 1712 1031
SfcI C'TryA_G
Cuts at: 826 1045 2398 5289 5748 5937 6469 7604 8285
Size: 219 1353 2891 459 189 532 1135 681
Cuts at: 8285 8476 826
Size: 191 1471
Fragments arranged by size:
2891 1471 1353 1135 681 532 459 219
191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9121
SgrAI Cr'CCGG_yG
Cuts at: 1700 2550 1700
Size: 850 8271
SmaI CCC'GGG
Cuts at: 1872 6448 1872
Size: 4576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4542 4839 7225 8126 8388 820
Size: 197 926 2599 297 2386 901 262 1553
Fragments arranged by size:
2599 2386 1553 926 901 297 262 197
SnaBI TAC'GTA
Cuts at: 493 6418 6912 493
Size: 5925 494 2702
Fragments arranged by size:
5925 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9121
SphI G_CATG'C
Cuts at: 1132 6479 1132
Size: 5347 3774
SspI AAT'ATT
Cuts at: 5 52 7045 5
Size: 47 6993 2081
Fragments arranged by size:
6993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9121
StyI C'CwwG_G
Cuts at: 513 1065 3207 4885 6560 513
Size: 552 2142 1678 1675 3074
Fragments arranged by size:
3074 2142 1678 1675 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5105 5495 7148 7165 7318 7503 7948
Size: 1255 3525 390 1653 17 153 185 445
Cuts at: 7948 325
Size: 1498
Fragments arranged by size:
3525 1653 1498 1255 445 390 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5067 6336 7367
Size: 274 80 33 51 1443 3090 1269 1031
Cuts at: 7367 9079 96
Size: 1712 138
Fragments arranged by size:
3090 1712 1443 1269 1031 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2655 2687 5078 5110 5269 5301 9081 9113 2655
Size: 32 2391 32 159 32 3780 32 2663
Fragments arranged by size:
3780 2663 2391 159 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4331 4910 1316
Size: 3015 579 5527
Fragments arranged by size:
5527 3015 579
XbaI T'CTAG_A
Cuts at: 6457 6457
Size: 9121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2181 2123
Size: 58 9063
XhoI C'TCGA_G
Cuts at: 1943 4542 1943
Size: 2599 6522
XmaI C'CCGG_G
Cuts at: 1870 6446 1870
Size: 4576 4545
XmnI GAAnn'nnTTC
Cuts at: 6338 7250 6338
Size: 912 8209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6352 6929 276
Size: 53 83 186 936 4818 577 2468
Fragments arranged by size:
4818 2468 936 577 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI