Restriction map for
pFN21ASDA0283
(Circular) (Six-base) MAPSORT of: pFN21ASDA0283.seq Check: 5067 from: 1 to: 9052
pFN21ASDA0283 9052 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4231 4231
Size: 9052
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4503 6862 278
Size: 53 83 186 936 2967 2359 2468
Fragments arranged by size:
2967 2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 3042 3210 5043 6312 6395 1937
Size: 451 654 168 1833 1269 83 4594
Fragments arranged by size:
4594 1833 1269 654 451 168 83
Acc65I G'GTAC_C
Cuts at: 6373 6373
Size: 9052
AclI AA'CG_TT
Cuts at: 1563 7179 7552 1563
Size: 5616 373 3063
Fragments arranged by size:
5616 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3008 3905 7115 8499 8767 1632
Size: 695 681 897 3210 1384 268 1917
Fragments arranged by size:
3210 1917 1384 897 695 681 268
AfeI AGC'GCT
Cuts at: 6074 6074
Size: 9052
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8855
AflIII A'CryG_T
Cuts at: 1203 6237 7951 8928 1203
Size: 5034 1714 977 1327
Fragments arranged by size:
5034 1714 1327 977
AgeI A'CCGG_T
Cuts at: 7883 7883
Size: 9052
AhdI GACnn_n'nnGTC
Cuts at: 4905 7781 4905
Size: 2876 6176
AleI CACnn'nnGTG
Cuts at: 1166 2744 5274 1166
Size: 1578 2530 4944
Fragments arranged by size:
4944 2530 1578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4911 1184
Size: 3727 5325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4772 4804 4772
Size: 32 9020
AlwNI CAG_nnn'CTG
Cuts at: 2142 2792 3482 6795 8367 2142
Size: 650 690 3313 1572 2827
Fragments arranged by size:
3313 2827 1572 690 650
ApaI G_GGCC'C
Cuts at: 3513 3750 5259 5386 5916 3513
Size: 237 1509 127 530 6649
Fragments arranged by size:
6649 1509 530 237 127
ApaLI G'TGCA_C
Cuts at: 5429 7109 8265 5429
Size: 1680 1156 6216
Fragments arranged by size:
6216 1680 1156
ApoI r'AATT_y
Cuts at: 1493 3777 4213 5555 6305 6361 6587 6739 8848
Size: 2284 436 1342 750 56 226 152 2109
Cuts at: 8848 1493
Size: 1697
Fragments arranged by size:
2284 2109 1697 1342 750 436 226 152
56
AseI AT'TA_AT
Cuts at: 160 1032 7606 160
Size: 872 6574 1606
Fragments arranged by size:
6574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9052
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 3422 3628 3870 4605 4803 5594
Size: 73 148 1331 206 242 735 198 791
Cuts at: 5594 6377 1870
Size: 783 4545
Fragments arranged by size:
4545 1331 791 783 735 242 206 198
148 73
BamHI G'GATC_C
Cuts at: 6382 6749 6382
Size: 367 8685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 3964 4199 6373 7828
Size: 325 217 627 300 1877 235 2174 1455
Cuts at: 7828 618
Size: 1842
Fragments arranged by size:
2174 1877 1842 1455 627 325 300 235
217
BanII G_rGCy'C
Cuts at: 1391 2098 2475 3223 3249 3513 3750 3880 4405
Size: 707 377 748 26 264 237 130 525
Cuts at: 4405 5082 5148 5259 5379 5386 5616 5916 6371
Size: 677 66 111 120 7 230 300 455
Cuts at: 6371 1391
Size: 4072
Fragments arranged by size:
4072 748 707 677 525 455 377 300
264 237 230 130 120 111 66 26
7
BbeI G_GCGC'C
Cuts at: 2091 2091
Size: 9052
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3474 5900 928
Size: 971 1575 2426 4080
Fragments arranged by size:
4080 2426 1575 971
BbvCI CC'TCA_GC
Cuts at: 2048 2048
Size: 9052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7243 7277 1903
Size: 34 5306 34 3678
Fragments arranged by size:
5306 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4865 6944 8154 4865
Size: 2079 1210 5763
Fragments arranged by size:
5763 2079 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5211 5460 7663
Size: 107 122 71 631 605 3539 249 2203
Cuts at: 7663 136
Size: 1525
Fragments arranged by size:
3539 2203 1525 631 605 249 122 107
71
BglII A'GATC_T
Cuts at: 3525 9047 3525
Size: 5522 3530
BlpI GC'TnA_GC
Cuts at: 4454 6469 4454
Size: 2015 7037
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3513 3750 3969 5259 5386 5433 5916
Size: 442 1281 237 219 1290 127 47 483
Cuts at: 5916 7113 8269 1790
Size: 1197 1156 2573
Fragments arranged by size:
2573 1290 1281 1197 1156 483 442 237
219 127 47
BmgBI CAC'GTC
Cuts at: 1318 5653 6238 1318
Size: 4335 585 4132
Fragments arranged by size:
4335 4132 585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5860 6783 7741
Size: 416 53 83 399 24 4443 923 958
Cuts at: 7741 442
Size: 1753
Fragments arranged by size:
4443 1753 958 923 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 5914 5950 3122
Size: 2792 36 6224
Fragments arranged by size:
6224 2792 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2335 2687 3973 5521 5824 6011 6122 7712
Size: 1096 352 1286 1548 303 187 111 1590
Cuts at: 7712 1239
Size: 2579
Fragments arranged by size:
2579 1590 1548 1286 1096 352 303 187
111
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 9052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4887 7177 8042 8340 4887
Size: 2290 865 298 5599
Fragments arranged by size:
5599 2290 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2685 3023 3548 4157 4382 4824 6281
Size: 617 1186 338 525 609 225 442 1457
Cuts at: 6281 7715 882
Size: 1434 2219
Fragments arranged by size:
2219 1457 1434 1186 617 609 525 442
338 225
BsaAI yAC'GTr
Cuts at: 493 1204 2392 3021 6349 6843 493
Size: 711 1188 629 3328 494 2702
Fragments arranged by size:
3328 2702 1188 711 629 494
BsaBI GATnn'nnATC
Cuts at: 3530 5637 6006 9046 3530
Size: 2107 369 3040 3536
Fragments arranged by size:
3536 3040 2107 369
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 4500 6859 7241
Size: 53 83 186 936 555 2412 2359 382
Cuts at: 7241 7876 275
Size: 635 1451
Fragments arranged by size:
2412 2359 1451 936 635 555 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3178 3657 7485 7883 8157 8304 8715 3178
Size: 479 3828 398 274 147 411 3515
Fragments arranged by size:
3828 3515 479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2333 2363 3867 3897 5813
Size: 30 165 30 930 30 1504 30 1916
Cuts at: 5813 5843 1178
Size: 30 4387
Fragments arranged by size:
4387 1916 1504 930 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3095 3343 3350 3458 3533 3926 4092 4274
Size: 1909 248 7 108 75 393 166 182
Cuts at: 4274 5426 5821 1186
Size: 1152 395 4417
Fragments arranged by size:
4417 1909 1152 395 393 248 182 166
108 75 7
BseYI C'CCAG_C
Cuts at: 2457 2796 4050 4539 5252 5279 5285 5769 8255
Size: 339 1254 489 713 27 6 484 2486
Cuts at: 8255 8751 2457
Size: 496 2758
Fragments arranged by size:
2758 2486 1254 713 496 489 484 339
27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3192 3314 3476 1163
Size: 573 1456 122 162 6739
Fragments arranged by size:
6739 1456 573 162 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6502 7263 8291 1540
Size: 275 186 4501 761 1028 2301
Fragments arranged by size:
4501 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2475 3105 3880 5148 5379 5433 7113 7198
Size: 342 630 775 1268 231 54 1680 85
Cuts at: 7198 8269 2133
Size: 1071 2916
Fragments arranged by size:
2916 1680 1268 1071 775 630 342 231
85 54
BsmI GAATG_Cn'
Cuts at: 2650 3988 6563 6656 2650
Size: 1338 2575 93 5046
Fragments arranged by size:
5046 2575 1338 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2530 5938 2530
Size: 3408 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2133 2232 2475 3105 3223 3249
Size: 399 308 35 99 243 630 118 26
Cuts at: 3249 3513 3750 3880 3969 4405 5082 5148 5259
Size: 264 237 130 89 436 677 66 111
Cuts at: 5259 5379 5386 5433 5616 5916 6371 7113 7198
Size: 120 7 47 183 300 455 742 85
Cuts at: 7198 8269 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 677 630 455 436 399
308 300 264 243 237 183 130 120
118 111 99 89 85 66 47 35
26 7
BspEI T'CCGG_A
Cuts at: 3657 8715 3657
Size: 5058 3994
BspHI T'CATG_A
Cuts at: 2244 3387 6941 8671 2244
Size: 1143 3554 1730 2625
Fragments arranged by size:
3554 2625 1730 1143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4047 4231 6407 844
Size: 3203 184 2176 3489
Fragments arranged by size:
3489 3203 2176 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2832 3863 6939 1394
Size: 429 1009 1031 3076 3507
Fragments arranged by size:
3507 3076 1031 1009 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 2714 2895 4474 4893 5304 7547
Size: 1183 1413 53 181 1579 419 411 2243
Cuts at: 7547 7721 65
Size: 174 1396
Fragments arranged by size:
2243 1579 1413 1396 1183 419 411 181
174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 7696 7883 1700
Size: 110 5886 187 2869
Fragments arranged by size:
5886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9052
BssSI C'ACGA_G
Cuts at: 7112 8124 7112
Size: 1012 8040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2792 794
Size: 1998 7054
BstBI TT'CG_AA
Cuts at: 2007 7856 2007
Size: 5849 3203
BstEII G'GTnAC_C
Cuts at: 1184 2737 3262 5764 1184
Size: 1553 525 2502 4472
Fragments arranged by size:
4472 2502 1553 525
BstXI CCAn_nnnn'nTGG
Cuts at: 4633 5791 5923 6035 6283 4633
Size: 1158 132 112 248 7402
Fragments arranged by size:
7402 1158 248 132 112
BstYI r'GATC_y
Cuts at: 1972 2237 3093 3175 3525 5136 6382 6749 7134
Size: 265 856 82 350 1611 1246 367 385
Cuts at: 7134 7151 8603 8689 8701 9047 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1611 1452 1246 856 385 367 350
346 265 86 82 17 12
BstZ17I GTA'TAC
Cuts at: 6313 6313
Size: 9052
Bsu36I CC'TnA_GG
Cuts at: 2060 2060
Size: 9052
BtgI C'CryG_G
Cuts at: 513 1065 2022 2556 4525 5179 5790 513
Size: 552 957 534 1969 654 611 3775
Fragments arranged by size:
3775 1969 957 654 611 552 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2536 487
Size: 683 56 1310 7003
Fragments arranged by size:
7003 1310 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 2232 2323 2790 3245 5269 5281 5466 5859
Size: 154 91 467 455 2024 12 185 393
Cuts at: 5859 6151 6205 6257 6568 7362 7382 2078
Size: 292 54 52 311 794 20 3748
Fragments arranged by size:
3748 2024 794 467 455 393 311 292
185 154 91 54 52 20 12
ClaI AT'CG_AT
Cuts at: 1554 4771 1554
Size: 3217 5835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2878 2913 3748 3783 4971 5006 529
Size: 35 2314 35 835 35 1188 35 4575
Fragments arranged by size:
4575 2314 1188 835 35 35 35 35
DraI TTT'AAA
Cuts at: 6356 6356
Size: 9052
DraIII CAC_nnn'GTG
Cuts at: 5585 6250 5585
Size: 665 8387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5039 5057 8059 809
Size: 507 3723 18 3002 1802
Fragments arranged by size:
3723 3002 1802 507 18
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 2492 4371
Size: 54 1425 50 275 18 445 217 1879
Cuts at: 4371 5024 5331 5993 6499 7388 9017 8
Size: 653 307 662 506 889 1629 43
Fragments arranged by size:
1879 1629 1425 889 662 653 506 445
307 275 217 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6499 1537
Size: 275 4687 4090
Fragments arranged by size:
4687 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4489 4661 5159 6982 1353
Size: 3136 172 498 1823 3423
Fragments arranged by size:
3423 3136 1823 498 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4951 5138 5210 5305 7639 8013
Size: 1064 138 3362 187 72 95 2334 374
Cuts at: 8013 8159 387
Size: 146 1280
Fragments arranged by size:
3362 2334 1280 1064 374 187 146 138
95 72
EcoICRI GAG'CTC
Cuts at: 2473 3878 5146 5377 2473
Size: 1405 1268 231 6148
Fragments arranged by size:
6148 1405 1268 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2335 2687 3008 3905 3973 5521
Size: 393 695 8 352 321 897 68 1548
Cuts at: 5521 5824 6011 6122 7115 7712 8499 8767 1239
Size: 303 187 111 993 597 787 268 1524
Fragments arranged by size:
1548 1524 993 897 787 695 597 393
352 321 303 268 187 111 68 8
EcoO109I rG'GnC_Cy
Cuts at: 2376 2572 3143 3509 3510 3747 4418 2376
Size: 196 571 366 1 237 671 7010
Fragments arranged by size:
7010 671 571 366 237 196 1
EcoRI G'AATT_C
Cuts at: 4213 6361 6739 4213
Size: 2148 378 6526
Fragments arranged by size:
6526 2148 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4359 4359
Size: 9052
FspI TGC'GCA
Cuts at: 7558 7558
Size: 9052
HaeII r_GCGC'y
Cuts at: 1678 2091 2106 2764 4461 6076 8199 1678
Size: 413 15 658 1697 1615 2123 2531
Fragments arranged by size:
2531 2123 1697 1615 658 413 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 3867 3899 4546
Size: 32 135 32 1256 32 1007 32 647
Cuts at: 4546 4578 4823 4855 4891 4923 5687 5719 7693
Size: 32 245 32 36 32 764 32 1974
Cuts at: 7693 7725 7767 7799 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1974 1256 1007 764 647 245 135
42 36 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 3043 4504 5395 6396 6642
Size: 988 281 814 291 1461 891 1001 246
Cuts at: 6642 669
Size: 3079
Fragments arranged by size:
3079 1461 1001 988 891 814 291 281
246
HindIII A'AGCT_T
Cuts at: 748 2693 748
Size: 1945 7107
HpaI GTT'AAC
Cuts at: 6642 6642
Size: 9052
KasI G'GCGC_C
Cuts at: 2087 2087
Size: 9052
KpnI G_GTAC'C
Cuts at: 6377 6377
Size: 9052
MfeI C'AATT_G
Cuts at: 6651 6651
Size: 9052
MluI A'CGCG_T
Cuts at: 8928 8928
Size: 9052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 4642 6118 6300 8166 8350 1305
Size: 1575 1762 1476 182 1866 184 2007
Fragments arranged by size:
2007 1866 1762 1575 1476 184 182
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2277 2494 4373 5026 10
Size: 54 1425 343 445 217 1879 653 4036
Fragments arranged by size:
4036 1879 1425 653 445 343 217 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2555 2656 2744 2935 3845 3848
Size: 648 1083 306 101 88 191 910 3
Cuts at: 3848 4631 4661 4973 5274 5477 5921 6236 7010
Size: 783 30 312 301 203 444 315 774
Cuts at: 7010 7369 7528 518
Size: 359 159 2042
Fragments arranged by size:
2042 1083 910 783 774 648 444 359
315 312 306 301 203 191 159 101
88 30 3
MspA1I CmG'CkG
Cuts at: 1606 2071 2800 2820 3229 3270 3898 3941 4527
Size: 465 729 20 409 41 628 43 586
Cuts at: 4527 5169 5670 6468 7145 8293 8538 1606
Size: 642 501 798 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 729 677 642 628 586
501 465 409 245 43 41 20
NarI GG'CG_CC
Cuts at: 2088 2088
Size: 9052
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7543
Fragments arranged by size:
7543 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9052
NotI GC'GGCC_GC
Cuts at: 6499 6499
Size: 9052
NspI r_CATG'y
Cuts at: 1132 1229 6410 7955 1132
Size: 97 5181 1545 2229
Fragments arranged by size:
5181 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2372 3695 1348
Size: 1024 1323 6705
Fragments arranged by size:
6705 1323 1024
PciI A'CATG_T
Cuts at: 7951 7951
Size: 9052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 6002 1108
Size: 1515 3379 4158
Fragments arranged by size:
4158 3379 1515
PfoI T'CCnGG_A
Cuts at: 3475 5139 5442 3475
Size: 1664 303 7085
Fragments arranged by size:
7085 1664 303
PmeI GTTT'AAAC
Cuts at: 6356 6356
Size: 9052
PmlI CAC'GTG
Cuts at: 3021 3021
Size: 9052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7121 7153 8634 8666 7121
Size: 32 1481 32 7507
Fragments arranged by size:
7507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 2572
Size: 9052
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9052
PsiI TTA'TAA
Cuts at: 6622 6622
Size: 9052
PspOMI G'GGCC_C
Cuts at: 3509 3746 5255 5382 5912 3509
Size: 237 1509 127 530 6649
Fragments arranged by size:
6649 1509 530 237 127
PstI C_TGCA'G
Cuts at: 830 2070 3240 4650 5276 5606 6200 6404 830
Size: 1240 1170 1410 626 330 594 204 3478
Fragments arranged by size:
3478 1410 1240 1170 626 594 330 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9052
PvuII CAG'CTG
Cuts at: 2071 3229 3898 3941 5169 2071
Size: 1158 669 43 1228 5954
Fragments arranged by size:
5954 1228 1158 669 43
SacI G_AGCT'C
Cuts at: 2475 3880 5148 5379 2475
Size: 1405 1268 231 6148
Fragments arranged by size:
6148 1405 1268 231
SacII CC_GC'GG
Cuts at: 4528 4528
Size: 9052
SalI G'TCGA_C
Cuts at: 1936 3041 6394 1936
Size: 1105 3353 4594
Fragments arranged by size:
4594 3353 1105
SbfI CC_TGCA'GG
Cuts at: 6404 6404
Size: 9052
ScaI AGT'ACT
Cuts at: 7300 9012 7300
Size: 1712 7340
SexAI A'CCwGG_T
Cuts at: 3115 4986 5397 3115
Size: 1871 411 6770
Fragments arranged by size:
6770 1871 411
SfcI C'TryA_G
Cuts at: 826 1045 2066 2134 3236 4123 4646 5272 5602
Size: 219 1021 68 1102 887 523 626 330
Cuts at: 5602 6196 6400 7535 8216 8407 826
Size: 594 204 1135 681 191 1471
Fragments arranged by size:
1471 1135 1102 1021 887 681 626 594
523 330 219 204 191 68
SfoI GGC'GCC
Cuts at: 2089 2089
Size: 9052
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9052
SmaI CCC'GGG
Cuts at: 1872 2093 3872 4607 4805 6379 1872
Size: 221 1779 735 198 1574 4545
Fragments arranged by size:
4545 1779 1574 735 221 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3628 4902 7156 8057 8319 820
Size: 197 926 1685 1274 2254 901 262 1553
Fragments arranged by size:
2254 1685 1553 1274 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6349 6843 493
Size: 5856 494 2702
Fragments arranged by size:
5856 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9052
SphI G_CATG'C
Cuts at: 1132 6410 1132
Size: 5278 3774
SrfI GCCC'GGGC
Cuts at: 2093 2093
Size: 9052
SspI AAT'ATT
Cuts at: 5 52 5970 6332 6976 5
Size: 47 5918 362 644 2081
Fragments arranged by size:
5918 2081 644 362 47
StuI AGG'CCT
Cuts at: 1517 3375 4706 1517
Size: 1858 1331 5863
Fragments arranged by size:
5863 1858 1331
StyI C'CwwG_G
Cuts at: 513 1065 2022 2413 3502 4743 6491 513
Size: 552 957 391 1089 1241 1748 3074
Fragments arranged by size:
3074 1748 1241 1089 957 552 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 2449 4055 4064 4223 5783 7079
Size: 1255 756 113 1606 9 159 1560 1296
Cuts at: 7079 7096 7249 7434 7879 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1606 1560 1498 1296 1255 756 445 185
159 153 113 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6030 7298 9010
Size: 274 80 33 51 1443 4053 1268 1712
Cuts at: 9010 96
Size: 138
Fragments arranged by size:
4053 1712 1443 1268 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3154 3186 6129 6161 9012 9044 3154
Size: 32 2943 32 2851 32 3162
Fragments arranged by size:
3162 2943 2851 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2564 1316
Size: 1248 7804
XbaI T'CTAG_A
Cuts at: 3490 6388 3490
Size: 2898 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 2779 4972 5206 5276 5326 6158 2711
Size: 68 2193 234 70 50 832 5605
Fragments arranged by size:
5605 2193 832 234 70 68 50
XhoI C'TCGA_G
Cuts at: 1943 3628 1943
Size: 1685 7367
XmaI C'CCGG_G
Cuts at: 1870 2091 3870 4605 4803 6377 1870
Size: 221 1779 735 198 1574 4545
Fragments arranged by size:
4545 1779 1574 735 221 198
XmnI GAAnn'nnTTC
Cuts at: 3471 3647 7181 3471
Size: 176 3534 5342
Fragments arranged by size:
5342 3534 176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4501 6860 276
Size: 53 83 186 936 2967 2359 2468
Fragments arranged by size:
2967 2468 2359 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SanDI SapI SfiI SwaI