Restriction map for
pFN21ASDA0280
(Circular) (Six-base) MAPSORT of: pFN21ASDA0280.seq Check: 2234 from: 1 to: 5461
pFN21ASDA0280 5461 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2804 1937
Size: 867 4594
Acc65I G'GTAC_C
Cuts at: 2645 2782 2645
Size: 137 5324
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 3524 4908 5176 1632
Size: 642 1250 1384 268 1917
Fragments arranged by size:
1917 1384 1250 642 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 2225
Size: 32 5429
AlwNI CAG_nnn'CTG
Cuts at: 2117 2185 2458 2573 3204 4776 2117
Size: 68 273 115 631 1572 2802
Fragments arranged by size:
2802 1572 631 273 115 68
ApaI G_GGCC'C
Cuts at: 2716 2716
Size: 5461
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2584 2786 1870
Size: 73 641 202 4545
Fragments arranged by size:
4545 641 202 73
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2621 2645 2782 4237
Size: 325 217 627 471 363 24 137 1455
Cuts at: 4237 618
Size: 1842
Fragments arranged by size:
1842 1455 627 471 363 325 217 137
24
BanII G_rGCy'C
Cuts at: 1391 2059 2716 2780 1391
Size: 668 657 64 4072
Fragments arranged by size:
4072 668 657 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 928
Size: 971 333 4157
Fragments arranged by size:
4157 971 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3353 4563 2436
Size: 917 1210 3334
Fragments arranged by size:
3334 1210 917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4072 136
Size: 107 122 71 631 605 2400 1525
Fragments arranged by size:
2400 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2686 2716 2745 3522 4678 1790
Size: 896 30 29 777 1156 2573
Fragments arranged by size:
2573 1156 896 777 30 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2154 2267 3192
Size: 416 53 83 399 24 737 113 925
Cuts at: 3192 4150 442
Size: 958 1753
Fragments arranged by size:
1753 958 925 737 416 399 113 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 4121 1239
Size: 915 1967 2579
Fragments arranged by size:
2579 1967 915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4124 882
Size: 617 2625 2219
Fragments arranged by size:
2625 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2740 2758 3252 493
Size: 711 1536 18 494 2702
Fragments arranged by size:
2702 1536 711 494 18
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3650 4285 275
Size: 53 83 186 936 1735 382 635 1451
Fragments arranged by size:
1735 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3894 4292 4566 4713 5124 3894
Size: 398 274 147 411 4231
Fragments arranged by size:
4231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2678 2708 1178
Size: 30 165 30 1275 30 3931
Fragments arranged by size:
3931 1275 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5461
BseYI C'CCAG_C
Cuts at: 2135 2448 2502 2566 2664 4664 5160 2135
Size: 313 54 64 98 2000 496 2436
Fragments arranged by size:
2436 2000 496 313 98 64 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 1163
Size: 573 330 4558
Fragments arranged by size:
4558 573 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2532 2911 3672 4700 1540
Size: 275 186 531 379 761 1028 2301
Fragments arranged by size:
2301 1028 761 531 379 275 186
BsiHKAI G_wGCw'C
Cuts at: 3522 3607 4678 3522
Size: 85 1071 4305
Fragments arranged by size:
4305 1071 85
BsmI GAATG_Cn'
Cuts at: 2188 2547 2972 3065 2188
Size: 359 425 93 4584
Fragments arranged by size:
4584 425 359 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2686 2716 2745 2780 3522 3607
Size: 399 269 627 30 29 35 742 85
Cuts at: 3607 4678 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 627 399 269 85 35
30 29
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2633 2816 844
Size: 1789 183 3489
Fragments arranged by size:
3489 1789 183
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 3956 4130 65
Size: 1183 1392 1316 174 1396
Fragments arranged by size:
1396 1392 1316 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2488 4105 4292 1700
Size: 110 678 1617 187 2869
Fragments arranged by size:
2869 1617 678 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2315 2576 794
Size: 1521 261 3679
Fragments arranged by size:
3679 1521 261
BstBI TT'CG_AA
Cuts at: 2007 4265 2007
Size: 2258 3203
BstEII G'GTnAC_C
Cuts at: 1184 2093 1184
Size: 909 4552
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2611 2599
Size: 12 5449
BstYI r'GATC_y
Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456
Size: 819 367 385 17 1452 86 12 346
Cuts at: 5456 1972
Size: 1977
Fragments arranged by size:
1977 1452 819 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2526 2604 2628 513
Size: 552 1461 78 24 3346
Fragments arranged by size:
3346 1461 552 78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2373 2977 3771 3791 2373
Size: 604 794 20 4043
Fragments arranged by size:
4043 794 604 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2225 2418 2601
Size: 54 1425 50 275 18 395 193 183
Cuts at: 2601 2908 3797 5426 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 889 395 307 275 193 183
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2908 1537
Size: 275 1096 4090
Fragments arranged by size:
4090 1096 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 1353
Size: 2038 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2274 3524 4121 4908 5176 1239
Size: 393 522 120 1250 597 787 268 1524
Fragments arranged by size:
1524 1250 787 597 522 393 268 120
EcoO109I rG'GnC_Cy
Cuts at: 2353 2712 2353
Size: 359 5102
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 4608 1678
Size: 2930 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373
Size: 32 135 32 2530 32 42 32 2626
Fragments arranged by size:
2626 2530 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KpnI G_GTAC'C
Cuts at: 2649 2786 2649
Size: 137 5324
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4575 4759 1305
Size: 3270 184 2007
Fragments arranged by size:
3270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2227 2603 10
Size: 54 1425 343 395 376 2868
Fragments arranged by size:
2868 1425 395 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 2627 2633 3419 3778 3937 518
Size: 648 1443 18 6 786 359 159 2042
Fragments arranged by size:
2042 1443 786 648 359 159 18 6
MspA1I CmG'CkG
Cuts at: 1606 2117 2877 3554 4702 4947 1606
Size: 511 760 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 677 511 245
NcoI C'CATG_G
Cuts at: 513 1065 2604 2628 513
Size: 552 1539 24 3346
Fragments arranged by size:
3346 1539 552 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2819 4364 1132
Size: 97 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2467 1348
Size: 1119 4342
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2165 2197 3530 3562 5043 5075 2165
Size: 32 1333 32 1481 32 2551
Fragments arranged by size:
2551 1481 1333 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 2353
Size: 5461
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PspOMI G'GGCC_C
Cuts at: 2712 2712
Size: 5461
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2018 2050 2069 2101 2285 2317 2018
Size: 32 19 32 184 32 5162
Fragments arranged by size:
5162 184 32 32 32 19
PstI C_TGCA'G
Cuts at: 830 2813 830
Size: 1983 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
PvuII CAG'CTG
Cuts at: 2117 2117
Size: 5461
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2809 3944 4625 4816 826
Size: 219 1764 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2788 1872
Size: 916 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2758 3252 493
Size: 2265 494 2702
Fragments arranged by size:
2702 2265 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 2222 1517
Size: 705 4756
StyI C'CwwG_G
Cuts at: 513 1065 2604 2628 2716 2900 513
Size: 552 1539 24 88 184 3074
Fragments arranged by size:
3074 1539 552 184 88 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2416 3488 3505 3658 3843 4288
Size: 1255 723 113 1072 17 153 185 445
Cuts at: 4288 325
Size: 1498
Fragments arranged by size:
1498 1255 1072 723 445 185 153 113
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2092 2124 2140 2172 2581 2613 2678 2710 5421
Size: 32 16 32 409 32 65 32 2711
Cuts at: 5421 5453 2092
Size: 32 2100
Fragments arranged by size:
2711 2100 409 65 32 32 32 32
32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2611 2611
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2786 1870
Size: 916 4545
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI