Restriction map for
pFN21ASDA0277
(Circular) (Six-base) MAPSORT of: pFN21ASDA0277.seq Check: 2612 from: 1 to: 6469
pFN21ASDA0277 6469 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2970 3370 3812 1937
Size: 1033 400 442 4594
Fragments arranged by size:
4594 1033 442 400
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2897 4532 5916 6184 1632
Size: 900 365 1635 1384 268 1917
Fragments arranged by size:
1917 1635 1384 900 365 268
AflII C'TTAA_G
Cuts at: 820 1017 2699 820
Size: 197 1682 4590
Fragments arranged by size:
4590 1682 197
AflIII A'CryG_T
Cuts at: 1203 2760 5368 6345 1203
Size: 1557 2608 977 1327
Fragments arranged by size:
2608 1557 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2418 3278 1184
Size: 1234 860 4375
Fragments arranged by size:
4375 1234 860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 2161
Size: 32 6437
AlwNI CAG_nnn'CTG
Cuts at: 2386 3611 3727 4212 5784 2386
Size: 1225 116 485 1572 3071
Fragments arranged by size:
3071 1572 1225 485 116
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 2606 2748 3085 3278 3322 3778 4004 4156
Size: 1113 142 337 193 44 456 226 152
Cuts at: 4156 6265 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1113 456 337 226 193 152
142 44
AseI AT'TA_AT
Cuts at: 160 1032 3080 5023 160
Size: 872 2048 1943 1606
Fragments arranged by size:
2048 1943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 3756 3794 1870
Size: 73 1002 811 38 4545
Fragments arranged by size:
4545 1002 811 73 38
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3790 5245 618
Size: 325 217 627 2003 1455 1842
Fragments arranged by size:
2003 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3738 3788 1391
Size: 2347 50 4072
Fragments arranged by size:
4072 2347 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2347 928
Size: 971 279 169 5050
Fragments arranged by size:
5050 971 279 169
BbvCI CC'TCA_GC
Cuts at: 2039 2039
Size: 6469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BfrBI ATG'CAT
Cuts at: 3476 3476
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3252 5080 136
Size: 107 122 71 631 605 1580 1828 1525
Fragments arranged by size:
1828 1580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 4530 5686 1790
Size: 2740 1156 2573
Fragments arranged by size:
2740 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 4200 5158
Size: 416 53 83 399 24 1056 1727 958
Cuts at: 5158 442
Size: 1753
Fragments arranged by size:
1753 1727 1056 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2449 2218
Size: 231 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 3176 5129 1239
Size: 1460 477 1953 2579
Fragments arranged by size:
2579 1953 1460 477
Bpu10I CC'TnA_GC
Cuts at: 2039 2039
Size: 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4594 5459 5757 4594
Size: 865 298 5306
Fragments arranged by size:
5306 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 3353 5132 882
Size: 617 813 1041 1779 2219
Fragments arranged by size:
2219 1779 1041 813 617
BsaAI yAC'GTr
Cuts at: 493 1204 2346 2761 3766 4260 493
Size: 711 1142 415 1005 494 2702
Fragments arranged by size:
2702 1142 1005 711 494 415
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4276 4658 5293 275
Size: 53 83 186 936 2743 382 635 1451
Fragments arranged by size:
2743 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2973 4902 5300 5574 5721 6132 2973
Size: 1929 398 274 147 411 3310
Fragments arranged by size:
3310 1929 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 1178
Size: 30 165 30 758 30 5456
Fragments arranged by size:
5456 758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 1186
Size: 976 5493
BseYI C'CCAG_C
Cuts at: 3250 5672 6168 3250
Size: 2422 496 3551
Fragments arranged by size:
3551 2422 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 1163
Size: 573 1548 4348
Fragments arranged by size:
4348 1548 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 2778 3738 4530 4615 5686 2271
Size: 507 960 792 85 1071 3054
Fragments arranged by size:
3054 1071 960 792 507 85
BsmI GAATG_Cn'
Cuts at: 2631 3476 3980 4073 2631
Size: 845 504 93 5027
Fragments arranged by size:
5027 845 504 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2778 3738 3788 4530 4615 5686
Size: 399 481 507 960 50 742 85 1071
Cuts at: 5686 1391
Size: 2174
Fragments arranged by size:
2174 1071 960 742 507 481 399 85
50
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 2358 3544 4358 6088 2358
Size: 1186 814 1730 2739
Fragments arranged by size:
2739 1730 1186 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3824 844
Size: 2980 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3208 4964 5138 65
Size: 1183 1960 1756 174 1396
Fragments arranged by size:
1960 1756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2493 96
Size: 2397 4072
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 3116 4529 5541 3116
Size: 1413 1012 4044
Fragments arranged by size:
4044 1413 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 2007 5273 2007
Size: 3266 3203
BstEII G'GTnAC_C
Cuts at: 1184 2505 3650 1184
Size: 1321 1145 4003
Fragments arranged by size:
4003 1321 1145
BstXI CCAn_nnnn'nTGG
Cuts at: 3343 3343
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 2823 2847 2866 3064 3442 3799 4166 4551
Size: 851 24 19 198 378 357 367 385
Cuts at: 4551 4568 6020 6106 6118 6464 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 851 385 378 367 357 346
198 86 24 19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2079 3225 513
Size: 552 957 57 1146 3757
Fragments arranged by size:
3757 1146 957 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 3168 3298 3354 3628 3985 4779 4799 3168
Size: 130 56 274 357 794 20 4838
Fragments arranged by size:
4838 794 357 274 130 56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 2538 3773 2538
Size: 1235 5234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2880 3222 3916
Size: 54 1425 50 275 18 1050 342 694
Cuts at: 3916 4805 6434 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1050 889 694 342 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2856 4399 1353
Size: 1503 1543 3423
Fragments arranged by size:
3423 1543 1503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 3002 5056 5430 5576 387
Size: 1064 138 476 937 2054 374 146 1280
Fragments arranged by size:
2054 1280 1064 937 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 3736 3736
Size: 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2699 2897 3176 4532 5129 5916
Size: 393 900 167 198 279 1356 597 787
Cuts at: 5916 6184 1239
Size: 268 1524
Fragments arranged by size:
1524 1356 900 787 597 393 279 268
198 167
EcoO109I rG'GnC_Cy
Cuts at: 2247 2247
Size: 6469
EcoRI G'AATT_C
Cuts at: 3322 3778 4156 3322
Size: 456 378 5635
Fragments arranged by size:
5635 456 378
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 2087 5616 1678
Size: 409 3529 2531
Fragments arranged by size:
3529 2531 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3668 3700 3739 3771 5110
Size: 32 135 32 2096 32 39 32 1339
Cuts at: 5110 5142 5184 5216 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2096 1339 135 42 39 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3371 3813 4059 669
Size: 988 281 1433 442 246 3079
Fragments arranged by size:
3079 1433 988 442 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5583 5767 1305
Size: 4278 184 2007
Fragments arranged by size:
4278 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 3224 10
Size: 54 1425 343 1050 342 3255
Fragments arranged by size:
3255 1425 1050 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2781 3341 3702 4427 4786 4945 518
Size: 648 1615 560 361 725 359 159 2042
Fragments arranged by size:
2042 1615 725 648 560 361 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 3250 3268 3885 4562 5710 5955 1606
Size: 475 1169 18 617 677 1148 245 2120
Fragments arranged by size:
2120 1169 1148 677 617 475 245 18
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4960
Fragments arranged by size:
4960 957 552
NdeI CA'TA_TG
Cuts at: 387 3597 387
Size: 3210 3259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NsiI A_TGCA'T
Cuts at: 3478 3478
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 3827 5372 1132
Size: 97 2598 1545 2229
Fragments arranged by size:
2598 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3398 1348
Size: 2050 4419
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PfoI T'CCnGG_A
Cuts at: 2243 2301 2325 2243
Size: 58 24 6387
Fragments arranged by size:
6387 58 24
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PmlI CAC'GTG
Cuts at: 2761 2761
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2193 4538 4570 6051 6083 2161
Size: 32 2345 32 1481 32 2547
Fragments arranged by size:
2547 2345 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2247
Size: 6469
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspXI vC'TCGA_Gb
Cuts at: 2945 2945
Size: 6469
PstI C_TGCA'G
Cuts at: 830 3245 3635 3821 830
Size: 2415 390 186 3478
Fragments arranged by size:
3478 2415 390 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 3250 3268 3250
Size: 18 6451
SacI G_AGCT'C
Cuts at: 3738 3738
Size: 6469
SacII CC_GC'GG
Cuts at: 2082 2082
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3369 3811 1936
Size: 1433 442 4594
Fragments arranged by size:
4594 1433 442
SanDI GG'GwC_CC
Cuts at: 2247 2247
Size: 6469
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 4717 6429 4717
Size: 1712 4757
SfcI C'TryA_G
Cuts at: 826 1045 2643 3241 3466 3631 3817 4952 5633
Size: 219 1598 598 225 165 186 1135 681
Cuts at: 5633 5824 826
Size: 191 1471
Fragments arranged by size:
1598 1471 1135 681 598 225 219 191
186 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 2945 4573 5474 5736 820
Size: 197 926 756 246 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 756 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3766 4260 493
Size: 3273 494 2702
Fragments arranged by size:
3273 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 2654 4393 5
Size: 47 2602 1739 2081
Fragments arranged by size:
2602 2081 1739 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2022 3908 513
Size: 552 957 1886 3074
Fragments arranged by size:
3074 1886 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2109 3208 4496 4513 4666 4851 5296
Size: 1255 529 1099 1288 17 153 185 445
Cuts at: 5296 325
Size: 1498
Fragments arranged by size:
1498 1288 1255 1099 529 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 2557 4715
Size: 274 80 33 51 1443 516 64 2158
Cuts at: 4715 6427 96
Size: 1712 138
Fragments arranged by size:
2158 1712 1443 516 274 138 80 64
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3486 3518 6429 6461 3486
Size: 32 2911 32 3494
Fragments arranged by size:
3494 2911 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2927 3611 1316
Size: 1611 684 4174
Fragments arranged by size:
4174 1611 684
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 2945 1943
Size: 1002 5467
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 3278 3326 3416 4598 3278
Size: 48 90 1182 5149
Fragments arranged by size:
5149 1182 90 48
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI
XcmI