Restriction map for
pFN21ASDA0275
(Circular) (Six-base) MAPSORT of: pFN21ASDA0275.seq Check: 1216 from: 1 to: 6001
pFN21ASDA0275 6001 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 2621 4832 2621
Size: 2211 3790
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AlwNI CAG_nnn'CTG
Cuts at: 2632 3744 5316 2632
Size: 1112 1572 3317
Fragments arranged by size:
3317 1572 1112
ApaI G_GGCC'C
Cuts at: 2558 2558
Size: 6001
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 3025 3169 3326 1870
Size: 73 89 993 144 157 4545
Fragments arranged by size:
4545 993 157 144 89 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3322 4777 618
Size: 325 217 627 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2109 2558 3143 3320 1391
Size: 718 449 585 177 4072
Fragments arranged by size:
4072 718 585 449 177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5030
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2266 3893 5103 2266
Size: 1627 1210 3164
Fragments arranged by size:
3164 1627 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2723 4612 136
Size: 107 122 71 631 605 1051 1889 1525
Fragments arranged by size:
1889 1525 1051 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 2701 3418 2701
Size: 717 5284
Bme1580I G_kGCm'C
Cuts at: 1790 2558 4062 5218 1790
Size: 768 1504 1156 2573
Fragments arranged by size:
2573 1504 1156 768
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 4690 442
Size: 416 53 83 399 24 2315 958 1753
Fragments arranged by size:
2315 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 2525 2962 2993 3131 4661 1239
Size: 1154 132 437 31 138 1530 2579
Fragments arranged by size:
2579 1530 1154 437 138 132 31
Bpu10I CC'TnA_GC
Cuts at: 2522 2522
Size: 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 4126 4991 5289 2093
Size: 2033 865 298 2805
Fragments arranged by size:
2805 2033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 2917 4664 882
Size: 617 615 803 1747 2219
Fragments arranged by size:
2219 1747 803 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 3298 3792 493
Size: 711 2094 494 2702
Fragments arranged by size:
2702 2094 711 494
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 3808 4190 4825
Size: 53 83 186 936 564 1711 382 635
Cuts at: 4825 275
Size: 1451
Fragments arranged by size:
1711 1451 936 635 564 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2621 3051 4434 4832 5106 5253 5664 2621
Size: 430 1383 398 274 147 411 2958
Fragments arranged by size:
2958 1383 430 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 1178
Size: 30 165 30 1002 30 4744
Fragments arranged by size:
4744 1002 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3221 3224 3251 3260 3263 3266 1186
Size: 2035 3 27 9 3 3 3921
Fragments arranged by size:
3921 2035 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2238 2448 3103 3195 5204 5700 2238
Size: 210 655 92 2009 496 2539
Fragments arranged by size:
2539 2009 655 496 210 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2326 1163
Size: 573 590 4838
Fragments arranged by size:
4838 590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2397 3451 4212 5240 1540
Size: 275 186 396 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 396 275 186
BsiHKAI G_wGCw'C
Cuts at: 2379 4062 4147 5218 2379
Size: 1683 85 1071 3162
Fragments arranged by size:
3162 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
BsmBI CGTCTCn'nnnn_
Cuts at: 2202 3212 2202
Size: 1010 4991
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2379 2558 3143 3320 4062 4147
Size: 399 319 270 179 585 177 742 85
Cuts at: 4147 5218 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 585 399 319 270 179
177 85
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 2677 3890 5620 2677
Size: 1213 1730 3058
Fragments arranged by size:
3058 1730 1213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3356 844
Size: 2512 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2156 2325 4496 4670 65
Size: 1183 908 169 2171 174 1396
Fragments arranged by size:
2171 1396 1183 908 174 169
BsrFI r'CCGG_y
Cuts at: 1700 1810 2621 2717 4645 4832 1700
Size: 110 811 96 1928 187 2869
Fragments arranged by size:
2869 1928 811 187 110 96
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 5900
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2436 794
Size: 1642 4359
BstBI TT'CG_AA
Cuts at: 2007 4805 2007
Size: 2798 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2845 2734
Size: 111 5890
BstYI r'GATC_y
Cuts at: 1972 2985 3331 3698 4083 4100 5552 5638 5650
Size: 1013 346 367 385 17 1452 86 12
Cuts at: 5650 5996 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1013 385 367 346 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 2407 2571 513
Size: 552 1008 334 164 3943
Fragments arranged by size:
3943 1008 552 334 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2560 3165 487
Size: 683 56 1334 605 3323
Fragments arranged by size:
3323 1334 683 605 56
BtsI GCAGTG_nn'
Cuts at: 2716 3068 3517 4311 4331 2716
Size: 352 449 794 20 4386
Fragments arranged by size:
4386 794 449 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DraIII CAC_nnn'GTG
Cuts at: 2320 2320
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2085 2476
Size: 54 1425 50 275 18 240 15 391
Cuts at: 2476 2724 2832 2905 3448 4337 5966 8
Size: 248 108 73 543 889 1629 43
Fragments arranged by size:
1629 1425 889 543 391 275 248 240
108 73 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2816 3931 1353
Size: 1463 1115 3423
Fragments arranged by size:
3423 1463 1115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2525 2962 2993 3131 4064 4661
Size: 393 761 132 437 31 138 933 597
Cuts at: 4661 5448 5716 1239
Size: 787 268 1524
Fragments arranged by size:
1524 933 787 761 597 437 393 268
138 132 31
EcoO109I rG'GnC_Cy
Cuts at: 2284 2554 2555 2629 2284
Size: 270 1 74 5656
Fragments arranged by size:
5656 270 74 1
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 5148 1678
Size: 3470 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4642 4674 4716 4748 1373
Size: 32 135 32 3070 32 42 32 2626
Fragments arranged by size:
3070 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2478 10
Size: 54 1425 343 646 3533
Fragments arranged by size:
3533 1425 646 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3959 4318 4477 518
Size: 648 2793 359 159 2042
Fragments arranged by size:
2793 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2075 2178 2344 2536 2730 2757 3078
Size: 453 16 103 166 192 194 27 321
Cuts at: 3078 3103 3417 4094 5242 5487 1606
Size: 25 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 321 314 245 194
192 166 103 27 25 16
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 6001
NcoI C'CATG_G
Cuts at: 513 1065 2407 513
Size: 552 1342 4107
Fragments arranged by size:
4107 1342 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 3359 4904 1132
Size: 97 2130 1545 2229
Fragments arranged by size:
2229 2130 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2332 1348
Size: 984 5017
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2775 1108
Size: 1667 4334
PfoI T'CCnGG_A
Cuts at: 2814 2988 2814
Size: 174 5827
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2629 2284
Size: 345 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2554 2554
Size: 6001
PstI C_TGCA'G
Cuts at: 830 3353 830
Size: 2523 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2536 2757 3103 2536
Size: 221 346 5434
Fragments arranged by size:
5434 346 221
SacII CC_GC'GG
Cuts at: 2076 2076
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SfcI C'TryA_G
Cuts at: 826 1045 3349 4484 5165 5356 826
Size: 219 2304 1135 681 191 1471
Fragments arranged by size:
2304 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 2034 3328 1872
Size: 162 1294 4545
Fragments arranged by size:
4545 1294 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 4105 5006 5268 820
Size: 197 926 165 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197 165
SnaBI TAC'GTA
Cuts at: 493 3298 3792 493
Size: 2805 494 2702
Fragments arranged by size:
2805 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2100 2280 2407 3440 513
Size: 552 1035 180 127 1033 3074
Fragments arranged by size:
3074 1035 1033 552 180 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4028 4045 4198 4383 4828 325
Size: 1255 2448 17 153 185 445 1498
Fragments arranged by size:
2448 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 4247 5959
Size: 274 80 33 51 1443 195 2075 1712
Cuts at: 5959 96
Size: 138
Fragments arranged by size:
2075 1712 1443 274 195 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 2032 3326 1870
Size: 162 1294 4545
Fragments arranged by size:
4545 1294 162
XmnI GAAnn'nnTTC
Cuts at: 2201 4130 2201
Size: 1929 4072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI