Restriction map for
pFN21ASDA0272
(Circular) (Six-base) MAPSORT of: pFN21ASDA0272.seq Check: 5693 from: 1 to: 6916
pFN21ASDA0272 6916 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3149 3149
Size: 6916
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4726 278
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2586 4259 1937
Size: 649 1673 4594
Fragments arranged by size:
4594 1673 649
Acc65I G'GTAC_C
Cuts at: 4237 4237
Size: 6916
AclI AA'CG_TT
Cuts at: 1563 5043 5416 1563
Size: 3480 373 3063
Fragments arranged by size:
3480 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2600 2786 3950 4979 6363 6631 1632
Size: 479 489 186 1164 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1164 1029 489 479 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6719
AflIII A'CryG_T
Cuts at: 1203 2259 5815 6792 1203
Size: 1056 3556 977 1327
Fragments arranged by size:
3556 1327 1056 977
AgeI A'CCGG_T
Cuts at: 5747 5747
Size: 6916
AhdI GACnn_n'nnGTC
Cuts at: 5645 5645
Size: 6916
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3719 1184
Size: 2535 4381
AlwNI CAG_nnn'CTG
Cuts at: 2806 2936 3091 4659 6231 2806
Size: 130 155 1568 1572 3491
Fragments arranged by size:
3491 1572 1568 155 130
ApaI G_GGCC'C
Cuts at: 2147 2607 2926 2147
Size: 460 319 6137
Fragments arranged by size:
6137 460 319
ApaLI G'TGCA_C
Cuts at: 4973 6129 4973
Size: 1156 5760
ApoI r'AATT_y
Cuts at: 1493 4225 4451 4603 6712 1493
Size: 2732 226 152 2109 1697
Fragments arranged by size:
2732 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5470 160
Size: 872 4438 1606
Fragments arranged by size:
4438 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
AvaI C'yCGr_G
Cuts at: 1870 1943 2462 4241 1870
Size: 73 519 1779 4545
Fragments arranged by size:
4545 1779 519 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2217 2250 2217
Size: 33 6883
BamHI G'GATC_C
Cuts at: 4246 4613 4246
Size: 367 6549
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 4122 4237 5692 618
Size: 325 217 627 935 1400 115 1455 1842
Fragments arranged by size:
1842 1455 1400 935 627 325 217 115
BanII G_rGCy'C
Cuts at: 1391 2147 2607 2688 2926 3885 4235 1391
Size: 756 460 81 238 959 350 4072
Fragments arranged by size:
4072 959 756 460 350 238 81
BbeI G_GCGC'C
Cuts at: 4126 4126
Size: 6916
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3153 3863 928
Size: 971 1254 710 3981
Fragments arranged by size:
3981 1254 971 710
BbvCI CC'TCA_GC
Cuts at: 3717 3717
Size: 6916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5107 5141 1903
Size: 34 3170 34 3678
Fragments arranged by size:
3678 3170 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4808 6018 4808
Size: 1210 5706
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 5527 136
Size: 107 122 71 631 605 2499 1356 1525
Fragments arranged by size:
2499 1525 1356 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2115 6911 2115
Size: 4796 2120
BlpI GC'TnA_GC
Cuts at: 2312 3559 4333 2312
Size: 1247 774 4895
Fragments arranged by size:
4895 1247 774
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2607 2727 2926 3459 4977 6133 1790
Size: 357 460 120 199 533 1518 1156 2573
Fragments arranged by size:
2573 1518 1156 533 460 357 199 120
BmgBI CAC'GTC
Cuts at: 1318 3247 1318
Size: 1929 4987
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3809 4647 5605
Size: 416 53 83 399 24 2392 838 958
Cuts at: 5605 442
Size: 1753
Fragments arranged by size:
2392 1753 958 838 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3919 3919
Size: 6916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2066 3021 3565 5576 1239
Size: 821 6 955 544 2011 2579
Fragments arranged by size:
2579 2011 955 821 544 6
Bpu10I CC'TnA_GC
Cuts at: 3717 4071 3717
Size: 354 6562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2835 3907 3956 5041 5906 6204 2835
Size: 1072 49 1085 865 298 3547
Fragments arranged by size:
3547 1085 1072 865 298 49
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2212 5579 882
Size: 617 713 3367 2219
Fragments arranged by size:
3367 2219 713 617
BsaAI yAC'GTr
Cuts at: 493 1204 4213 4707 493
Size: 711 3009 494 2702
Fragments arranged by size:
3009 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6910 6910
Size: 6916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4123 4723 5105 5740
Size: 53 83 186 936 2590 600 382 635
Cuts at: 5740 275
Size: 1451
Fragments arranged by size:
2590 1451 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5349 5747 6021 6168 6579 5349
Size: 398 274 147 411 5686
Fragments arranged by size:
5686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2460 2490 2864 2894 1178
Size: 30 165 30 1057 30 374 30 5200
Fragments arranged by size:
5200 1057 374 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2636 2885 2978 3251 3992 1186
Size: 940 510 249 93 273 741 4110
Fragments arranged by size:
4110 940 741 510 273 249 93
BseYI C'CCAG_C
Cuts at: 3195 3434 3485 6119 6615 3195
Size: 239 51 2634 496 3496
Fragments arranged by size:
3496 2634 496 239 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2523 2979 3269 3692 4033 1163
Size: 573 787 456 290 423 341 4046
Fragments arranged by size:
4046 787 573 456 423 341 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 4144 4366 5127 6155 1540
Size: 275 186 730 1413 222 761 1028 2301
Fragments arranged by size:
2301 1413 1028 761 730 275 222 186
BsiHKAI G_wGCw'C
Cuts at: 2322 4977 5062 6133 2322
Size: 2655 85 1071 3105
Fragments arranged by size:
3105 2655 1071 85
BsmI GAATG_Cn'
Cuts at: 4427 4520 4427
Size: 93 6823
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2322 2607 2688 2727 2926 3459
Size: 399 357 175 285 81 39 199 533
Cuts at: 3459 3885 4235 4977 5062 6133 1391
Size: 426 350 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 533 426 399 357 350
285 199 175 85 81 39
BspEI T'CCGG_A
Cuts at: 6579 6579
Size: 6916
BspHI T'CATG_A
Cuts at: 4805 6535 4805
Size: 1730 5186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3149 4271 844
Size: 2305 1122 3489
Fragments arranged by size:
3489 2305 1122
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 3558 4803 1394
Size: 429 1489 246 1245 3507
Fragments arranged by size:
3507 1489 1245 429 246
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 3496 5411 5585 65
Size: 1183 1177 1071 1915 174 1396
Fragments arranged by size:
1915 1396 1183 1177 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 2556 3094 4143 4170 5560 5747
Size: 110 380 366 538 1049 27 1390 187
Cuts at: 5747 1700
Size: 2869
Fragments arranged by size:
2869 1390 1049 538 380 366 187 110
27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6916
BssSI C'ACGA_G
Cuts at: 2075 4976 5988 2075
Size: 2901 1012 3003
Fragments arranged by size:
3003 2901 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2303 794
Size: 1509 5407
BstBI TT'CG_AA
Cuts at: 2007 5720 2007
Size: 3713 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6916
BstXI CCAn_nnnn'nTGG
Cuts at: 2954 3025 4096 2954
Size: 71 1071 5774
Fragments arranged by size:
5774 1071 71
BstYI r'GATC_y
Cuts at: 1972 2115 4246 4613 4998 5015 6467 6553 6565
Size: 143 2131 367 385 17 1452 86 12
Cuts at: 6565 6911 1972
Size: 346 1977
Fragments arranged by size:
2131 1977 1452 385 367 346 143 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2868 3269 2868
Size: 401 6515
BtgI C'CryG_G
Cuts at: 513 1065 2514 2599 3840 513
Size: 552 1449 85 1241 3589
Fragments arranged by size:
3589 1449 1241 552 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6177
Fragments arranged by size:
6177 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 2673 2724 3459 3529 3539 3923 4432 5226
Size: 162 51 735 70 10 384 509 794
Cuts at: 5226 5246 2511
Size: 20 4181
Fragments arranged by size:
4181 794 735 509 384 162 70 51
20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6916
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3061 3096 529
Size: 35 2497 35 4349
Fragments arranged by size:
4349 2497 35 35
DraI TTT'AAA
Cuts at: 4220 4220
Size: 6916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2845 5923 809
Size: 507 1529 3078 1802
Fragments arranged by size:
3078 1802 1529 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 4086 4141
Size: 54 1425 50 275 18 603 1653 55
Cuts at: 4141 4363 5252 6881 8
Size: 222 889 1629 43
Fragments arranged by size:
1653 1629 1425 889 603 275 222 55
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4141 4363 1537
Size: 275 2329 222 4090
Fragments arranged by size:
4090 2329 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2178 3983 4846 1353
Size: 716 109 1805 863 3423
Fragments arranged by size:
3423 1805 863 716 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3176 4189 5503 5877 6023 387
Size: 1064 138 1587 1013 1314 374 146 1280
Fragments arranged by size:
1587 1314 1280 1064 1013 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2066 2111 2600 2786 3021 3565
Size: 393 428 6 45 489 186 235 544
Cuts at: 3565 3950 4979 5576 6363 6631 1239
Size: 385 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 787 597 544 489 428 393
385 268 235 186 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2550 3716 3974 2550
Size: 1166 258 5492
Fragments arranged by size:
5492 1166 258
EcoO109I rG'GnC_Cy
Cuts at: 2143 2144 2348 2604 2922 2923 3394 3683 4023
Size: 1 204 256 318 1 471 289 340
Cuts at: 4023 2143
Size: 5036
Fragments arranged by size:
5036 471 340 318 289 256 204 1
1
EcoRI G'AATT_C
Cuts at: 4225 4603 4225
Size: 378 6538
FspI TGC'GCA
Cuts at: 2983 5422 2983
Size: 2439 4477
FspAI rTGC'GCAy
Cuts at: 2983 2983
Size: 6916
HaeII r_GCGC'y
Cuts at: 1678 2191 3746 4126 4207 6063 1678
Size: 513 1555 380 81 1856 2531
Fragments arranged by size:
2531 1856 1555 513 380 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 2177 2209 2213
Size: 32 135 32 550 32 23 32 4
Cuts at: 2213 2245 2864 2896 3168 3200 3494 3526 5557
Size: 32 619 32 272 32 294 32 2031
Cuts at: 5557 5589 5631 5663 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2031 619 550 294 272 135 42
32 32 32 32 32 32 32 32
32 32 23 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3646 4260 4506 669
Size: 988 281 1708 614 246 3079
Fragments arranged by size:
3079 1708 988 614 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6916
HpaI GTT'AAC
Cuts at: 4506 4506
Size: 6916
KasI G'GCGC_C
Cuts at: 4122 4122
Size: 6916
KpnI G_GTAC'C
Cuts at: 4241 4241
Size: 6916
MfeI C'AATT_G
Cuts at: 4515 4515
Size: 6916
MluI A'CGCG_T
Cuts at: 6792 6792
Size: 6916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6030 6214 1305
Size: 4725 184 2007
Fragments arranged by size:
4725 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 4088 10
Size: 54 1425 343 603 1653 2838
Fragments arranged by size:
2838 1653 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2952 3117 4112 4874 5233 5392 518
Size: 648 1786 165 995 762 359 159 2042
Fragments arranged by size:
2042 1786 995 762 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2278 2806 2863 2902 3088 3429 4159 4332
Size: 672 528 57 39 186 341 730 173
Cuts at: 4332 5009 6157 6402 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 730 677 672 528 341 245
186 173 57 39
NaeI GCC'GGC
Cuts at: 2558 3096 4145 2558
Size: 538 1049 5329
Fragments arranged by size:
5329 1049 538
NarI GG'CG_CC
Cuts at: 4123 4123
Size: 6916
NcoI C'CATG_G
Cuts at: 513 1065 2514 2599 513
Size: 552 1449 85 4830
Fragments arranged by size:
4830 1449 552 85
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6916
NgoMIV G'CCGG_C
Cuts at: 2556 3094 4143 2556
Size: 538 1049 5329
Fragments arranged by size:
5329 1049 538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6916
NotI GC'GGCC_GC
Cuts at: 4363 4363
Size: 6916
NspI r_CATG'y
Cuts at: 1132 1229 2263 2762 4084 4274 5819 1132
Size: 97 1034 499 1322 190 1545 2229
Fragments arranged by size:
2229 1545 1322 1034 499 190 97
PasI CC'CwG_GG
Cuts at: 1348 2607 1348
Size: 1259 5657
PciI A'CATG_T
Cuts at: 2259 5815 2259
Size: 3556 3360
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6916
PmeI GTTT'AAAC
Cuts at: 4220 4220
Size: 6916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 4985 5017 6498 6530 3245
Size: 32 1708 32 1481 32 3631
Fragments arranged by size:
3631 1708 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2348 3394 3683 4023 2348
Size: 1046 289 340 5241
Fragments arranged by size:
5241 1046 340 289
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6916
PsiI TTA'TAA
Cuts at: 4486 4486
Size: 6916
PspOMI G'GGCC_C
Cuts at: 2143 2603 2922 2143
Size: 460 319 6137
Fragments arranged by size:
6137 460 319
PstI C_TGCA'G
Cuts at: 830 2333 2680 2802 2932 3345 4268 830
Size: 1503 347 122 130 413 923 3478
Fragments arranged by size:
3478 1503 923 413 347 130 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6916
PvuII CAG'CTG
Cuts at: 2278 2806 2863 2278
Size: 528 57 6331
Fragments arranged by size:
6331 528 57
SalI G'TCGA_C
Cuts at: 1936 4258 1936
Size: 2322 4594
SanDI GG'GwC_CC
Cuts at: 2348 2348
Size: 6916
SapI GCTCTTCn'nnn_
Cuts at: 2178 2178
Size: 6916
SbfI CC_TGCA'GG
Cuts at: 4268 4268
Size: 6916
ScaI AGT'ACT
Cuts at: 2787 3203 5164 6876 2787
Size: 416 1961 1712 2827
Fragments arranged by size:
2827 1961 1712 416
SfcI C'TryA_G
Cuts at: 826 1045 2329 2676 2798 2928 3341 4264 5399
Size: 219 1284 347 122 130 413 923 1135
Cuts at: 5399 6080 6271 826
Size: 681 191 1471
Fragments arranged by size:
1471 1284 1135 923 681 413 347 219
191 130 122
SfoI GGC'GCC
Cuts at: 4124 4124
Size: 6916
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6916
SmaI CCC'GGG
Cuts at: 1872 4243 1872
Size: 2371 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2850 3886 3971 5020 5921 6183
Size: 197 926 907 1036 85 1049 901 262
Cuts at: 6183 820
Size: 1553
Fragments arranged by size:
1553 1049 1036 926 907 901 262 197
85
SnaBI TAC'GTA
Cuts at: 493 4213 4707 493
Size: 3720 494 2702
Fragments arranged by size:
3720 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6916
SphI G_CATG'C
Cuts at: 1132 4084 4274 1132
Size: 2952 190 3774
Fragments arranged by size:
3774 2952 190
SspI AAT'ATT
Cuts at: 5 52 4840 5
Size: 47 4788 2081
Fragments arranged by size:
4788 2081 47
StuI AGG'CCT
Cuts at: 1517 2061 3702 3874 1517
Size: 544 1641 172 4559
Fragments arranged by size:
4559 1641 544 172
StyI C'CwwG_G
Cuts at: 513 1065 2214 2379 2436 2514 2599 3606 3790
Size: 552 1149 165 57 78 85 1007 184
Cuts at: 3790 3909 4126 4355 513
Size: 119 217 229 3074
Fragments arranged by size:
3074 1149 1007 552 229 217 184 165
119 85 78 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3493 3922 4943 4960 5113 5298 5743
Size: 1255 1913 429 1021 17 153 185 445
Cuts at: 5743 325
Size: 1498
Fragments arranged by size:
1913 1498 1255 1021 445 429 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2785 3201 3497
Size: 274 80 33 51 1443 808 416 296
Cuts at: 3497 5162 6874 96
Size: 1665 1712 138
Fragments arranged by size:
1712 1665 1443 808 416 296 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2460 2492 2609 2641 6876 6908 2460
Size: 32 117 32 4235 32 2468
Fragments arranged by size:
4235 2468 117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6916
XbaI T'CTAG_A
Cuts at: 3103 4252 3103
Size: 1149 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3361 3814 3916 3361
Size: 453 102 6361
Fragments arranged by size:
6361 453 102
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6916
XmaI C'CCGG_G
Cuts at: 1870 4241 1870
Size: 2371 4545
XmnI GAAnn'nnTTC
Cuts at: 2372 3998 5045 2372
Size: 1626 1047 4243
Fragments arranged by size:
4243 1626 1047
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4724 276
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI
SfiI SrfI SwaI