Restriction map for
pFN21ASDA0269
(Circular) (Six-base) MAPSORT of: pFN21ASDA0269.seq Check: 1560 from: 1 to: 6406
pFN21ASDA0269 6406 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2197 2486 2685 3131 4469 5853 6121
Size: 555 10 289 199 446 1338 1384 268
Cuts at: 6121 1632
Size: 1917
Fragments arranged by size:
1917 1384 1338 555 446 289 268 199
10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 2236 5305 6282 1203
Size: 1033 3069 977 1327
Fragments arranged by size:
3069 1327 1033 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2387 1184
Size: 1203 5203
AlwNI CAG_nnn'CTG
Cuts at: 2641 3104 3289 4149 5721 2641
Size: 463 185 860 1572 3326
Fragments arranged by size:
3326 1572 860 463 185
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 3674 3715 3941 4093 6202 1493
Size: 2181 41 226 152 2109 1697
Fragments arranged by size:
2181 2109 1697 226 152 41
AseI AT'TA_AT
Cuts at: 160 1032 3428 4960 160
Size: 872 2396 1532 1606
Fragments arranged by size:
2396 1606 1532 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3731 1870
Size: 73 1788 4545
Fragments arranged by size:
4545 1788 73
AvrII C'CTAG_G
Cuts at: 2051 2051
Size: 6406
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3727 5182 618
Size: 325 217 627 1940 1455 1842
Fragments arranged by size:
1940 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2987 3725 1391
Size: 1596 738 4072
Fragments arranged by size:
4072 1596 738
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5435
BbvCI CC'TCA_GC
Cuts at: 2823 2823
Size: 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BfrBI ATG'CAT
Cuts at: 2875 2875
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3588 3823 3588
Size: 235 6171
Bme1580I G_kGCm'C
Cuts at: 1790 4467 5623 1790
Size: 2677 1156 2573
Fragments arranged by size:
2677 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3230 4137 5095
Size: 416 53 83 399 24 1813 907 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1813 1753 958 907 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3006 3006
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3057 3081 3102 3120 3180 5066 1239
Size: 1818 24 21 18 60 1886 2579
Fragments arranged by size:
2579 1886 1818 60 24 21 18
Bpu10I CC'TnA_GC
Cuts at: 2823 2823
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2749 3703 4197 493
Size: 711 1545 954 494 2702
Fragments arranged by size:
2702 1545 954 711 494
BsaBI GATnn'nnATC
Cuts at: 2676 6400 2676
Size: 3724 2682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3046 3076 1178
Size: 30 165 30 1643 30 4508
Fragments arranged by size:
4508 1643 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2991 3054 3132 3168 3171 3186 3303 3432
Size: 1805 63 78 36 3 15 117 129
Cuts at: 3432 1186
Size: 4160
Fragments arranged by size:
4160 1805 129 117 78 63 36 15
3
BseYI C'CCAG_C
Cuts at: 2713 3172 5609 6105 2713
Size: 459 2437 496 3014
Fragments arranged by size:
3014 2437 496 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5833
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2362 3856 4617 5645 1540
Size: 275 186 361 1494 761 1028 2301
Fragments arranged by size:
2301 1494 1028 761 361 275 186
BsiHKAI G_wGCw'C
Cuts at: 2611 4467 4552 5623 2611
Size: 1856 85 1071 3394
Fragments arranged by size:
3394 1856 1071 85
BsiWI C'GTAC_G
Cuts at: 2394 2394
Size: 6406
BsmI GAATG_Cn'
Cuts at: 3330 3917 4010 3330
Size: 587 93 5726
Fragments arranged by size:
5726 587 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2384 2384
Size: 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2611 2987 3725 4467 4552 5623 1391
Size: 399 821 376 738 742 85 1071 2174
Fragments arranged by size:
2174 1071 821 742 738 399 376 85
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 2584 2614 4295 6025 2584
Size: 30 1681 1730 2965
Fragments arranged by size:
2965 1730 1681 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3469 3761 844
Size: 2625 292 3489
Fragments arranged by size:
3489 2625 292
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2225 4901 5075 65
Size: 1183 977 2676 174 1396
Fragments arranged by size:
2676 1396 1183 977 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5050 5237 1700
Size: 110 3240 187 2869
Fragments arranged by size:
3240 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2888 794
Size: 2094 4312
BstBI TT'CG_AA
Cuts at: 2007 5210 2007
Size: 3203 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 2765 3736 4103 4488 4505 5957 6043 6055
Size: 793 971 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 971 793 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2072 3102 3922 4716 4736 2072
Size: 1030 820 794 20 3742
Fragments arranged by size:
3742 1030 820 794 20
ClaI AT'CG_AT
Cuts at: 1554 2046 1554
Size: 492 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DraIII CAC_nnn'GTG
Cuts at: 3275 3275
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3853 4742 6371
Size: 54 1425 50 275 18 2023 889 1629
Cuts at: 6371 8
Size: 43
Fragments arranged by size:
2023 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3018 3150 3561 4336 1353
Size: 1665 132 411 775 3423
Fragments arranged by size:
3423 1665 775 411 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2197 2486 2685 3057 3081 3102
Size: 393 555 10 289 199 372 24 21
Cuts at: 3102 3120 3131 3180 4469 5066 5853 6121 1239
Size: 18 11 49 1289 597 787 268 1524
Fragments arranged by size:
1524 1289 787 597 555 393 372 289
268 199 49 24 21 18 11 10
EcoO109I rG'GnC_Cy
Cuts at: 3413 3413
Size: 6406
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 3481 5553 1678
Size: 1803 2072 2531
Fragments arranged by size:
2531 2072 1803
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2923 2955 3046
Size: 32 135 32 662 32 657 32 91
Cuts at: 3046 3078 5047 5079 5121 5153 1373
Size: 32 1969 32 42 32 2626
Fragments arranged by size:
2626 1969 662 657 135 91 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 3030 3750 3996 669
Size: 988 281 283 809 720 246 3079
Fragments arranged by size:
3079 988 809 720 283 281 246
HindIII A'AGCT_T
Cuts at: 748 2319 748
Size: 1571 4835
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 3186 4005 3186
Size: 819 5587
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3416 3675 5520 5704 1305
Size: 2111 259 1845 184 2007
Fragments arranged by size:
2111 2007 1845 259 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4584
Fragments arranged by size:
4584 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2760 4364 4723 4882 518
Size: 648 1594 1604 359 159 2042
Fragments arranged by size:
2042 1604 1594 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3822 4499 5647 5892 1606
Size: 2216 677 1148 245 2120
Fragments arranged by size:
2216 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5854
NdeI CA'TA_TG
Cuts at: 387 2757 387
Size: 2370 4036
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NsiI A_TGCA'T
Cuts at: 2877 2877
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2876 1108
Size: 1768 4638
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3413 3413
Size: 6406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PstI C_TGCA'G
Cuts at: 830 3758 830
Size: 2928 3478
PvuI CG_AT'CG
Cuts at: 2001 2362 2001
Size: 361 6045
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 3561 3561
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2922 2958 3754 4889 5570 5761 826
Size: 219 1877 36 796 1135 681 191 1471
Fragments arranged by size:
1877 1471 1135 796 681 219 191 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4510 5411 5673 820
Size: 197 926 2567 901 262 1553
Fragments arranged by size:
2567 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3703 4197 493
Size: 3210 494 2702
Fragments arranged by size:
3210 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 2116 4330 5
Size: 47 2064 2214 2081
Fragments arranged by size:
2214 2081 2064 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2051 2121 2647 3845 513
Size: 552 986 70 526 1198 3074
Fragments arranged by size:
3074 1198 986 552 526 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3186 4433 4450 4603 4788 5233 325
Size: 1255 1606 1247 17 153 185 445 1498
Fragments arranged by size:
1606 1498 1255 1247 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3202 4652 6364
Size: 274 80 33 51 1443 1225 1450 1712
Cuts at: 6364 96
Size: 138
Fragments arranged by size:
1712 1450 1443 1225 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6366 6398 6366
Size: 32 6374
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 2572 3742 2572
Size: 1170 5236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2119
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 2321 4535 2321
Size: 2214 4192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI