Restriction map for
pFN21ASDA0264
(Circular) (Six-base) MAPSORT of: pFN21ASDA0264.seq Check: 1342 from: 1 to: 5971
pFN21ASDA0264 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2127 3314 1937
Size: 190 1187 4594
Fragments arranged by size:
4594 1187 190
Acc65I G'GTAC_C
Cuts at: 2328 3292 2328
Size: 964 5007
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 2593 2880 2895 2903 4034 5418 5686
Size: 468 493 287 15 8 1131 1384 268
Cuts at: 5686 1632
Size: 1917
Fragments arranged by size:
1917 1384 1131 493 468 287 268 15
8
AfeI AGC'GCT
Cuts at: 2859 2859
Size: 5971
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 3157 3714 5286 3157
Size: 557 1572 3842
Fragments arranged by size:
3842 1572 557
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2430 3280 3506 3658 5767 1493
Size: 937 850 226 152 2109 1697
Fragments arranged by size:
2109 1697 937 850 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 3296 1870
Size: 73 322 1031 4545
Fragments arranged by size:
4545 1031 322 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 3292 4747 618
Size: 325 217 627 541 964 1455 1842
Fragments arranged by size:
1842 1455 964 627 541 325 217
BanII G_rGCy'C
Cuts at: 1391 2800 3290 1391
Size: 1409 490 4072
Fragments arranged by size:
4072 1409 490
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2606 2841 2943 928
Size: 971 707 235 102 3956
Fragments arranged by size:
3956 971 707 235 102
BbvCI CC'TCA_GC
Cuts at: 2079 2079
Size: 5971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 4032 5188 1790
Size: 2242 1156 2573
Fragments arranged by size:
2573 2242 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 3702 4660
Size: 416 53 83 399 24 1269 1016 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1269 1016 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2876
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4631 1239
Size: 3392 2579
Bpu10I CC'TnA_GC
Cuts at: 2079 2079
Size: 5971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3268 3762 493
Size: 711 2064 494 2702
Fragments arranged by size:
2702 2064 711 494
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 2930 1186
Size: 1450 294 4227
Fragments arranged by size:
4227 1450 294
BseYI C'CCAG_C
Cuts at: 3093 5174 5670 3093
Size: 2081 496 3394
Fragments arranged by size:
3394 2081 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4032 4117 5188 4032
Size: 85 1071 4815
Fragments arranged by size:
4815 1071 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2800 3290 4032 4117 5188 1391
Size: 399 1010 490 742 85 1071 2174
Fragments arranged by size:
2174 1071 1010 742 490 399 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 2523 3860 5590 2523
Size: 1337 1730 2904
Fragments arranged by size:
2904 1730 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 2115 3326 844
Size: 1240 31 1211 3489
Fragments arranged by size:
3489 1240 1211 31
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2584 4466 4640 65
Size: 1183 1336 1882 174 1396
Fragments arranged by size:
1882 1396 1336 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 3181 96
Size: 3085 2886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3157 794
Size: 2363 3608
BstBI TT'CG_AA
Cuts at: 2007 4775 2007
Size: 2768 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstXI CCAn_nnnn'nTGG
Cuts at: 2699 2699
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966
Size: 1329 367 385 17 1452 86 12 346
Cuts at: 5966 1972
Size: 1977
Fragments arranged by size:
1977 1452 1329 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4462
Fragments arranged by size:
4462 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3121 487
Size: 683 56 1895 3337
Fragments arranged by size:
3337 1895 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 2883 3170 3487 4281 4301 2372
Size: 511 287 317 794 20 4042
Fragments arranged by size:
4042 794 511 317 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3079 3114 529
Size: 35 2515 35 3386
Fragments arranged by size:
3386 2515 35 35
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2613 3901 1353
Size: 912 348 1288 3423
Fragments arranged by size:
3423 1288 912 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2593 2880 2895 2903 4034 4631
Size: 393 468 493 287 15 8 1131 597
Cuts at: 4631 5418 5686 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1131 787 597 493 468 393 287
268 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2359 2359
Size: 5971
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2090 2623 2090
Size: 533 5438
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2861 5118 1678
Size: 1183 2257 2531
Fragments arranged by size:
2531 2257 1183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 2489 2521 2906
Size: 32 135 32 596 32 289 32 385
Cuts at: 2906 2938 4612 4644 4686 4718 1373
Size: 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 596 385 289 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2999 3041 748
Size: 2251 42 3678
Fragments arranged by size:
3678 2251 42
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 2332 3296 2332
Size: 964 5007
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3929 4288 4447 518
Size: 648 2763 359 159 2042
Fragments arranged by size:
2763 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2900 3387 4064 5212 5457 1606
Size: 1294 487 677 1148 245 2120
Fragments arranged by size:
2120 1294 1148 677 487 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4462
Fragments arranged by size:
4462 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2764 3329 4874 1132
Size: 97 1535 565 1545 2229
Fragments arranged by size:
2229 1545 1535 565 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2325 1108
Size: 1217 4754
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2403 2435 2403
Size: 32 5939
PstI C_TGCA'G
Cuts at: 830 2095 3162 3323 830
Size: 1265 1067 161 3478
Fragments arranged by size:
3478 1265 1067 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2900 2900
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 2095 3323 2095
Size: 1228 4743
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2091 3158 3319 4454 5135 5326 826
Size: 219 1046 1067 161 1135 681 191 1471
Fragments arranged by size:
1471 1135 1067 1046 681 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3268 3762 493
Size: 2775 494 2702
Fragments arranged by size:
2775 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 2658 3029 1517
Size: 1141 371 4459
Fragments arranged by size:
4459 1141 371
StyI C'CwwG_G
Cuts at: 513 1065 2022 3410 513
Size: 552 957 1388 3074
Fragments arranged by size:
3074 1388 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2570 3107 3998 4015 4168 4353 4798
Size: 1255 990 537 891 17 153 185 445
Cuts at: 4798 325
Size: 1498
Fragments arranged by size:
1498 1255 990 891 537 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3181 4217 5929
Size: 274 80 33 51 1443 1204 1036 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1712 1443 1204 1036 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2620 2652 5931 5963 2620
Size: 32 3279 32 2628
Fragments arranged by size:
3279 2628 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3172 3307 3172
Size: 135 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI