Restriction map for
pFN21ASDA0258
(Circular) (Six-base) MAPSORT of: pFN21ASDA0258.seq Check: 1827 from: 1 to: 5902
pFN21ASDA0258 5902 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2427 3096 3245 1937
Size: 490 669 149 4594
Fragments arranged by size:
4594 669 490 149
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 3088 3965 5349 5617 1632
Size: 866 590 877 1384 268 1917
Fragments arranged by size:
1917 1384 877 866 590 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2869 4801 5778 1203
Size: 1666 1932 977 1327
Fragments arranged by size:
1932 1666 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 2274 1166
Size: 1108 4794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3080 3645 5217 3080
Size: 565 1572 3765
Fragments arranged by size:
3765 1572 565
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2313 3002 3211 3437 3589 5698 1493
Size: 820 689 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 820 689 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 3015 3227 1870
Size: 73 1072 212 4545
Fragments arranged by size:
4545 1072 212 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2886 3223 4678 618
Size: 325 217 627 1099 337 1455 1842
Fragments arranged by size:
1842 1455 1099 627 337 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 3221 1391
Size: 655 1175 4072
Fragments arranged by size:
4072 1175 655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2303 3794 5004 2303
Size: 1491 1210 3201
Fragments arranged by size:
3201 1491 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 2045 2607 3319 2045
Size: 562 712 4628
Fragments arranged by size:
4628 712 562
Bme1580I G_kGCm'C
Cuts at: 1790 2164 3963 5119 1790
Size: 374 1799 1156 2573
Fragments arranged by size:
2573 1799 1156 374
BmgBI CAC'GTC
Cuts at: 1318 2737 2840 1318
Size: 1419 103 4380
Fragments arranged by size:
4380 1419 103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2606 1056
Size: 1550 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2364 4562 1239
Size: 863 262 2198 2579
Fragments arranged by size:
2579 2198 863 262
Bpu10I CC'TnA_GC
Cuts at: 3081 3081
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3199 3693 493
Size: 711 1995 494 2702
Fragments arranged by size:
2702 1995 711 494
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2523 4335 4733 5007 5154 5565 2523
Size: 1812 398 274 147 411 2860
Fragments arranged by size:
2860 1812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2202 2585 2822 1186
Size: 1016 383 237 4266
Fragments arranged by size:
4266 1016 383 237
BseYI C'CCAG_C
Cuts at: 2069 2615 3108 3173 5105 5601 2069
Size: 546 493 65 1932 496 2370
Fragments arranged by size:
2370 1932 546 496 493 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 3963 4048 5119 2046
Size: 1917 85 1071 2829
Fragments arranged by size:
2829 1917 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2164 3221 3963 4048 5119 1391
Size: 399 256 118 1057 742 85 1071 2174
Fragments arranged by size:
2174 1071 1057 742 399 256 118 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3123 3257 844
Size: 2279 134 3489
Fragments arranged by size:
3489 2279 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 2718 2815 2963 96
Size: 2622 97 148 3035
Fragments arranged by size:
3035 2622 148 97
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 2007 4706 2007
Size: 2699 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897
Size: 1260 367 385 17 1452 86 12 346
Cuts at: 5897 1972
Size: 1977
Fragments arranged by size:
1977 1452 1260 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2330 2330
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 4297
Fragments arranged by size:
4297 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 2204 3418 4212 4232 2170
Size: 34 1214 794 20 3840
Fragments arranged by size:
3840 1214 794 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2677 3349
Size: 54 1425 50 275 18 291 556 672
Cuts at: 3349 4238 5867 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 672 556 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3832 1353
Size: 2479 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2103 4489 4863 5009 387
Size: 1064 138 514 2386 374 146 1280
Fragments arranged by size:
2386 1280 1064 514 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2044
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2364 2498 3088 3965 4562 5349
Size: 393 470 262 134 590 877 597 787
Cuts at: 5349 5617 1239
Size: 268 1524
Fragments arranged by size:
1524 877 787 597 590 470 393 268
262 134
EcoO109I rG'GnC_Cy
Cuts at: 2054 2143 2745 3077 3112 3170 2054
Size: 89 602 332 35 58 4786
Fragments arranged by size:
4786 602 332 89 58 35
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2081 5049 1678
Size: 403 2968 2531
Fragments arranged by size:
2968 2531 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2339 2355 2371 3018
Size: 32 135 32 751 16 16 16 647
Cuts at: 3018 3050 4543 4575 4617 4649 1373
Size: 32 1493 32 42 32 2626
Fragments arranged by size:
2626 1493 751 647 135 42 32 32
32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 3246 3492 669
Size: 988 281 520 788 246 3079
Fragments arranged by size:
3079 988 788 520 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2274 3125 3860 4219 4378 518
Size: 648 1021 87 851 735 359 159 2042
Fragments arranged by size:
2042 1021 851 735 648 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2833 3108 3318 3995 5143 5388 1606
Size: 1227 275 210 677 1148 245 2120
Fragments arranged by size:
2120 1227 1148 677 275 245 210
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5350
NdeI CA'TA_TG
Cuts at: 387 3157 387
Size: 2770 3132
NheI G'CTAG_C
Cuts at: 1052 2602 1052
Size: 1550 4352
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2873 3260 4805 1132
Size: 97 1644 387 1545 2229
Fragments arranged by size:
2229 1644 1545 387 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 2869 4801 2869
Size: 1932 3970
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PfoI T'CCnGG_A
Cuts at: 2341 2341
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 2745 3077 3112 2054
Size: 691 332 35 4844
Fragments arranged by size:
4844 691 332 35
PshAI GACnn'nnGTC
Cuts at: 1613 2223 1613
Size: 610 5292
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 3108 3108
Size: 5902
SacI G_AGCT'C
Cuts at: 2046 2046
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2365 2544 3093 3250 4385 5066 5257
Size: 219 1320 179 549 157 1135 681 191
Cuts at: 5257 826
Size: 1471
Fragments arranged by size:
1471 1320 1135 681 549 219 191 179
157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3199 3693 493
Size: 2706 494 2702
Fragments arranged by size:
2706 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 3341 513
Size: 552 2276 3074
Fragments arranged by size:
3074 2276 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2819 3929 3946 4099 4284 4729 325
Size: 1255 1239 1110 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1239 1110 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2718 2815 2963
Size: 274 80 33 51 1443 741 97 148
Cuts at: 2963 4148 5860 96
Size: 1185 1712 138
Fragments arranged by size:
1712 1443 1185 741 274 148 138 97
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2879 2911 5862 5894 2879
Size: 32 2951 32 2887
Fragments arranged by size:
2951 2887 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2143 2563 2837 2029
Size: 114 420 274 5094
Fragments arranged by size:
5094 420 274 114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI