Restriction map for
pFN21ASDA0252
(Circular) (Six-base) MAPSORT of: pFN21ASDA0252.seq Check: 7921 from: 1 to: 6739
pFN21ASDA0252 6739 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2195 4549 278
Size: 53 83 186 936 659 2354 2468
Fragments arranged by size:
2468 2354 936 659 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2835 4082 1937
Size: 898 1247 4594
Fragments arranged by size:
4594 1247 898
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2991 3473 4802 6186 6454 1632
Size: 1359 482 1329 1384 268 1917
Fragments arranged by size:
1917 1384 1359 1329 482 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 3205 5638 6615 1203
Size: 2002 2433 977 1327
Fragments arranged by size:
2433 2002 1327 977
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3314 1184
Size: 2130 4609
AlwNI CAG_nnn'CTG
Cuts at: 2351 2375 3451 4482 6054 2351
Size: 24 1076 1031 1572 3036
Fragments arranged by size:
3036 1572 1076 1031 24
ApaI G_GGCC'C
Cuts at: 2485 2485
Size: 6739
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 3239 3359 3841 4048 4274 4426 6535 1493
Size: 1746 120 482 207 226 152 2109 1697
Fragments arranged by size:
2109 1746 1697 482 226 207 152 120
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 4064 1870
Size: 73 2121 4545
Fragments arranged by size:
4545 2121 73
BamHI G'GATC_C
Cuts at: 3676 4069 4436 3676
Size: 393 367 5979
Fragments arranged by size:
5979 393 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3158 4060 5515 618
Size: 325 217 627 1371 902 1455 1842
Fragments arranged by size:
1842 1455 1371 902 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 2345 2485 2799 3982 4058 1391
Size: 715 239 140 314 1183 76 4072
Fragments arranged by size:
4072 1183 715 314 239 140 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2460 2481 3446 4011 928
Size: 971 561 21 965 565 3656
Fragments arranged by size:
3656 971 965 565 561 21
BbvCI CC'TCA_GC
Cuts at: 3471 3471
Size: 6739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 1903
Size: 34 2993 34 3678
Fragments arranged by size:
3678 2993 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 3367 1548
Size: 1819 4920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3641 3752 5350
Size: 107 122 71 631 605 1969 111 1598
Cuts at: 5350 136
Size: 1525
Fragments arranged by size:
1969 1598 1525 631 605 122 111 107
71
BglII A'GATC_T
Cuts at: 3871 3989 6734 3871
Size: 118 2745 3876
Fragments arranged by size:
3876 2745 118
BlpI GC'TnA_GC
Cuts at: 2344 2793 4156 2344
Size: 449 1363 4927
Fragments arranged by size:
4927 1363 449
Bme1580I G_kGCm'C
Cuts at: 1790 2485 4800 5956 1790
Size: 695 2315 1156 2573
Fragments arranged by size:
2573 2315 1156 695
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4470 5428 442
Size: 416 53 83 399 24 3053 958 1753
Fragments arranged by size:
3053 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2854 3244 2474
Size: 380 390 5969
Fragments arranged by size:
5969 390 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3548 3801 5399 1239
Size: 2309 253 1598 2579
Fragments arranged by size:
2579 2309 1598 253
Bpu10I CC'TnA_GC
Cuts at: 3471 3471
Size: 6739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2724 4864 5729 6027 2724
Size: 2140 865 298 3436
Fragments arranged by size:
3436 2140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3720 5402 882
Size: 617 2221 1682 2219
Fragments arranged by size:
2221 2219 1682 617
BsaAI yAC'GTr
Cuts at: 493 1204 4036 4530 493
Size: 711 2832 494 2702
Fragments arranged by size:
2832 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3071 6733 3071
Size: 3662 3077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2192 4546 4928 5563
Size: 53 83 186 936 659 2354 382 635
Cuts at: 5563 275
Size: 1451
Fragments arranged by size:
2354 1451 936 659 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 1178
Size: 30 165 30 690 30 5794
Fragments arranged by size:
5794 690 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2296 2380 2651 2765 2867 2939 1186
Size: 970 140 84 271 114 102 72 4986
Fragments arranged by size:
4986 970 271 140 114 102 84 72
BseYI C'CCAG_C
Cuts at: 3730 3756 3920 5942 6438 3730
Size: 26 164 2022 496 4031
Fragments arranged by size:
4031 2022 496 164 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2345 2799 4800 4885 5956 2106
Size: 239 454 2001 85 1071 2889
Fragments arranged by size:
2889 2001 1071 454 239 85
BsmI GAATG_Cn'
Cuts at: 3769 4250 4343 3769
Size: 481 93 6165
Fragments arranged by size:
6165 481 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2183 2183
Size: 6739
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2345 2485 2799 3982 4058 4800
Size: 399 316 239 140 314 1183 76 742
Cuts at: 4800 4885 5956 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1183 1071 742 399 316 314 239
140 85 76
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 4628 6358 4628
Size: 1730 5009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4094 844
Size: 3250 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2892 4626 1394
Size: 429 1069 1734 3507
Fragments arranged by size:
3507 1734 1069 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3202 5234 5408 65
Size: 1183 1954 2032 174 1396
Fragments arranged by size:
2032 1954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5383 5570 1700
Size: 110 3573 187 2869
Fragments arranged by size:
3573 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6739
BstBI TT'CG_AA
Cuts at: 2007 5543 2007
Size: 3536 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 3155 3155
Size: 6739
BstYI r'GATC_y
Cuts at: 1972 2937 3676 3871 3989 4069 4436 4821 4838
Size: 965 739 195 118 80 367 385 17
Cuts at: 4838 6290 6376 6388 6734 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 965 739 385 367 346 195
118 86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2308 2352 2308
Size: 44 6695
BtgI C'CryG_G
Cuts at: 513 1065 2022 2745 3465 513
Size: 552 957 723 720 3787
Fragments arranged by size:
3787 957 723 720 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3290 487
Size: 683 56 2064 3936
Fragments arranged by size:
3936 2064 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 3701 4255 5049 5069 2137
Size: 1564 554 794 20 3807
Fragments arranged by size:
3807 1564 794 554 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4186 5075 6704
Size: 54 1425 50 275 18 2356 889 1629
Cuts at: 6704 8
Size: 43
Fragments arranged by size:
2356 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2398 2419 2424 2537 2628 2844 2853 2916
Size: 1045 21 5 113 91 216 9 63
Cuts at: 2916 2928 3375 4669 1353
Size: 12 447 1294 3423
Fragments arranged by size:
3423 1294 1045 447 216 113 91 63
21 12 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3951 5326 5700 5846 387
Size: 1064 138 2362 1375 374 146 1280
Fragments arranged by size:
2362 1375 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2104 2343 2797 2104
Size: 239 454 6046
Fragments arranged by size:
6046 454 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2991 3473 3548 3801 4802 5399 6186
Size: 393 1359 482 75 253 1001 597 787
Cuts at: 6186 6454 1239
Size: 268 1524
Fragments arranged by size:
1524 1359 1001 787 597 482 393 268
253 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2355 2096
Size: 259 6480
EcoO109I rG'GnC_Cy
Cuts at: 2481 3475 3544 3634 2481
Size: 994 69 90 5586
Fragments arranged by size:
5586 994 90 69
EcoRI G'AATT_C
Cuts at: 3239 3841 4048 4426 3239
Size: 602 207 378 5552
Fragments arranged by size:
5552 602 378 207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3365 3925 3365
Size: 560 6179
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 5886 1678
Size: 4208 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2396 2402 2428
Size: 32 135 32 521 32 271 6 26
Cuts at: 2428 2434 2556 2588 2720 2752 2855 2879 2887
Size: 6 122 32 132 32 103 24 8
Cuts at: 2887 2911 3802 3834 5380 5412 5454 5486 1373
Size: 24 891 32 1546 32 42 32 2626
Fragments arranged by size:
2626 1546 891 521 271 135 132 122
103 42 32 32 32 32 32 32
32 32 26 24 24 8 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4083 4329 669
Size: 988 281 2145 246 3079
Fragments arranged by size:
3079 2145 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 3205 6615 3205
Size: 3410 3329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5853 6037 1305
Size: 4548 184 2007
Fragments arranged by size:
4548 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4917
Fragments arranged by size:
4917 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3195 4697 5056 5215 518
Size: 648 2029 1502 359 159 2042
Fragments arranged by size:
2042 2029 1502 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2336 2792 3559 3598 3734 3760 4155
Size: 470 260 456 767 39 136 26 395
Cuts at: 4155 4832 5980 6225 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 470 456 395 260
245 136 39 26
NcoI C'CATG_G
Cuts at: 513 1065 2022 2745 3465 513
Size: 552 957 723 720 3787
Fragments arranged by size:
3787 957 723 720 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 3028 3295 4097 5642 1132
Size: 97 1799 267 802 1545 2229
Fragments arranged by size:
2229 1799 1545 802 267 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6739
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3673 1108
Size: 2565 4174
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3475 3475
Size: 6739
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 3357 4309 3357
Size: 952 5787
PspOMI G'GGCC_C
Cuts at: 2481 2481
Size: 6739
PstI C_TGCA'G
Cuts at: 830 2173 4091 830
Size: 1343 1918 3478
Fragments arranged by size:
3478 1918 1343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2336 2792 3734 3760 2336
Size: 456 942 26 5315
Fragments arranged by size:
5315 942 456 26
SacI G_AGCT'C
Cuts at: 2106 2345 2799 2106
Size: 239 454 6046
Fragments arranged by size:
6046 454 239
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 4987 6699 4987
Size: 1712 5027
SfcI C'TryA_G
Cuts at: 826 1045 2169 4087 5222 5903 6094 826
Size: 219 1124 1918 1135 681 191 1471
Fragments arranged by size:
1918 1471 1135 1124 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2739 4843 5744 6006 820
Size: 197 926 796 2104 901 262 1553
Fragments arranged by size:
2104 1553 926 901 796 262 197
SnaBI TAC'GTA
Cuts at: 493 4036 4530 493
Size: 3543 494 2702
Fragments arranged by size:
3543 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 3295 4097 1132
Size: 2163 802 3774
Fragments arranged by size:
3774 2163 802
SspI AAT'ATT
Cuts at: 5 52 4663 5
Size: 47 4611 2081
Fragments arranged by size:
4611 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2022 2745 3465 3954 3969 3983 4178
Size: 552 957 723 720 489 15 14 195
Cuts at: 4178 513
Size: 3074
Fragments arranged by size:
3074 957 723 720 552 489 195 15
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3436 4766 4783 4936 5121 5566 325
Size: 1255 1856 1330 17 153 185 445 1498
Fragments arranged by size:
1856 1498 1330 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3883 4985 6697
Size: 274 80 33 51 1443 1906 1102 1712
Cuts at: 6697 96
Size: 138
Fragments arranged by size:
1906 1712 1443 1102 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3045 3077 6699 6731 3045
Size: 32 3622 32 3053
Fragments arranged by size:
3622 3053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 3307 4075 3307
Size: 768 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2444 2444
Size: 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2193 4547 276
Size: 53 83 186 936 659 2354 2468
Fragments arranged by size:
2468 2354 936 659 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI