Restriction map for
pFN21ASDA0250
(Circular) (Six-base) MAPSORT of: pFN21ASDA0250.seq Check: 1787 from: 1 to: 8002
pFN21ASDA0250 8002 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5812 278
Size: 53 83 186 936 4276 2468
Fragments arranged by size:
4276 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4148 5345 1937
Size: 2211 1197 4594
Fragments arranged by size:
4594 2211 1197
Acc65I G'GTAC_C
Cuts at: 5323 5323
Size: 8002
AclI AA'CG_TT
Cuts at: 1563 6129 6502 1563
Size: 4566 373 3063
Fragments arranged by size:
4566 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2124 3786 3956 4334 6065 7449 7717
Size: 455 37 1662 170 378 1731 1384 268
Cuts at: 7717 1632
Size: 1917
Fragments arranged by size:
1917 1731 1662 1384 455 378 268 170
37
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7805
AflIII A'CryG_T
Cuts at: 1203 6901 7878 1203
Size: 5698 977 1327
Fragments arranged by size:
5698 1327 977
AgeI A'CCGG_T
Cuts at: 6833 6833
Size: 8002
AhdI GACnn_n'nnGTC
Cuts at: 3149 6731 3149
Size: 3582 4420
AleI CACnn'nnGTG
Cuts at: 1166 2946 1166
Size: 1780 6222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8002
AlwNI CAG_nnn'CTG
Cuts at: 2128 2137 3016 3764 5745 7317 2128
Size: 9 879 748 1981 1572 2813
Fragments arranged by size:
2813 1981 1572 879 748 9
ApaLI G'TGCA_C
Cuts at: 6059 7215 6059
Size: 1156 6846
ApoI r'AATT_y
Cuts at: 1493 2786 3616 4487 5311 5537 5689 7798 1493
Size: 1293 830 871 824 226 152 2109 1697
Fragments arranged by size:
2109 1697 1293 871 830 824 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3497 6556 160
Size: 872 2465 3059 1606
Fragments arranged by size:
3059 2465 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8002
AvaI C'yCGr_G
Cuts at: 1870 1943 5327 1870
Size: 73 3384 4545
Fragments arranged by size:
4545 3384 73
BamHI G'GATC_C
Cuts at: 4600 5332 5699 4600
Size: 732 367 6903
Fragments arranged by size:
6903 732 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5120 5276 5323 6778 618
Size: 325 217 627 3333 156 47 1455 1842
Fragments arranged by size:
3333 1842 1455 627 325 217 156 47
BanII G_rGCy'C
Cuts at: 1391 2834 3584 3926 4656 5321 1391
Size: 1443 750 342 730 665 4072
Fragments arranged by size:
4072 1443 750 730 665 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2382 3513 928
Size: 971 483 1131 5417
Fragments arranged by size:
5417 1131 971 483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6193 6227 1903
Size: 34 4256 34 3678
Fragments arranged by size:
4256 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5894 7104 5894
Size: 1210 6792
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8002
BfrBI ATG'CAT
Cuts at: 5209 5209
Size: 8002
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6613 136
Size: 107 122 71 631 605 4941 1525
Fragments arranged by size:
4941 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4526 7997 4526
Size: 3471 4531
BlpI GC'TnA_GC
Cuts at: 2559 4131 5419 2559
Size: 1572 1288 5142
Fragments arranged by size:
5142 1572 1288
Bme1580I G_kGCm'C
Cuts at: 1790 6063 7219 1790
Size: 4273 1156 2573
Fragments arranged by size:
4273 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8002
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 3811 4504
Size: 416 53 83 399 24 2090 304 693
Cuts at: 4504 5733 6691 442
Size: 1229 958 1753
Fragments arranged by size:
2090 1753 1229 958 693 416 399 304
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8002
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 4663 5173 3259
Size: 1404 510 6088
Fragments arranged by size:
6088 1404 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3741 3861 3975 4971 5249 6662 1239
Size: 2502 120 114 996 278 1413 2579
Fragments arranged by size:
2579 2502 1413 996 278 120 114
Bpu10I CC'TnA_GC
Cuts at: 3925 3925
Size: 8002
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3229 3786 6127 6992 7290 3229
Size: 557 2341 865 298 3941
Fragments arranged by size:
3941 2341 865 557 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2616 3124 3298 3690 4848 6665 882
Size: 617 1117 508 174 392 1158 1817 2219
Fragments arranged by size:
2219 1817 1158 1117 617 508 392 174
BsaAI yAC'GTr
Cuts at: 493 1204 5299 5793 493
Size: 711 4095 494 2702
Fragments arranged by size:
4095 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7996 7996
Size: 8002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5809 6191 6826 275
Size: 53 83 186 936 4276 382 635 1451
Fragments arranged by size:
4276 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4688 6435 6833 7107 7254 7665 4688
Size: 1747 398 274 147 411 5025
Fragments arranged by size:
5025 1747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5222 5252 1178
Size: 30 165 30 3819 30 3928
Fragments arranged by size:
3928 3819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3095 3287 3843 4884 4920 1186
Size: 1909 192 556 1041 36 4268
Fragments arranged by size:
4268 1909 1041 556 192 36
BseYI C'CCAG_C
Cuts at: 2092 2680 3860 3940 4013 4104 4922 7205 7701
Size: 588 1180 80 73 91 818 2283 496
Cuts at: 7701 2092
Size: 2393
Fragments arranged by size:
2393 2283 1180 818 588 496 91 80
73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5452 6213 7241 1540
Size: 275 186 3451 761 1028 2301
Fragments arranged by size:
3451 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4656 6063 6148 7219 4656
Size: 1407 85 1071 5439
Fragments arranged by size:
5439 1407 1071 85
BsmI GAATG_Cn'
Cuts at: 4620 5513 5606 4620
Size: 893 93 7016
Fragments arranged by size:
7016 893 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2834 3584 3926 4656 5321 6063 6148
Size: 399 1044 750 342 730 665 742 85
Cuts at: 6148 7219 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1044 750 742 730 665 399
342 85
BspEI T'CCGG_A
Cuts at: 7665 7665
Size: 8002
BspHI T'CATG_A
Cuts at: 5891 7621 5891
Size: 1730 6272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4153 5357 844
Size: 3309 1204 3489
Fragments arranged by size:
3489 3309 1204
BsrBI CCG'CTC
Cuts at: 1394 1823 5889 1394
Size: 429 4066 3507
Fragments arranged by size:
4066 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2502 2658 2965 4385 6497 6671 65
Size: 1183 1254 156 307 1420 2112 174 1396
Fragments arranged by size:
2112 1420 1396 1254 1183 307 174 156
BsrFI r'CCGG_y
Cuts at: 1700 1810 4210 6646 6833 1700
Size: 110 2400 2436 187 2869
Fragments arranged by size:
2869 2436 2400 187 110
BsrGI T'GTAC_A
Cuts at: 96 2351 96
Size: 2255 5747
BssHII G'CGCG_C
Cuts at: 1925 2037 1925
Size: 112 7890
BssSI C'ACGA_G
Cuts at: 3681 6062 7074 3681
Size: 2381 1012 4609
Fragments arranged by size:
4609 2381 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2128 3048 794
Size: 1334 920 5748
Fragments arranged by size:
5748 1334 920
BstBI TT'CG_AA
Cuts at: 2007 6806 2007
Size: 4799 3203
BstEII G'GTnAC_C
Cuts at: 1184 3338 3655 4572 1184
Size: 2154 317 917 4614
Fragments arranged by size:
4614 2154 917 317
BstYI r'GATC_y
Cuts at: 1972 2197 4526 4600 5332 5699 6084 6101 7553
Size: 225 2329 74 732 367 385 17 1452
Cuts at: 7553 7639 7651 7997 1972
Size: 86 12 346 1977
Fragments arranged by size:
2329 1977 1452 732 385 367 346 225
86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 4296 4296
Size: 8002
BtgI C'CryG_G
Cuts at: 513 1065 2791 3123 4443 4838 5040 5134 5145
Size: 552 1726 332 1320 395 202 94 11
Cuts at: 5145 513
Size: 3370
Fragments arranged by size:
3370 1726 1320 552 395 332 202 94
11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7263
Fragments arranged by size:
7263 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 3171 5518 6312 6332 2479
Size: 692 2347 794 20 4149
Fragments arranged by size:
4149 2347 794 692 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7967
DraI TTT'AAA
Cuts at: 5306 5306
Size: 8002
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3760 4840 7009 809
Size: 507 2444 1080 2169 1802
Fragments arranged by size:
2444 2169 1802 1080 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5449 6338 7967
Size: 54 1425 50 275 18 3619 889 1629
Cuts at: 7967 8
Size: 43
Fragments arranged by size:
3619 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5449 1537
Size: 275 3637 4090
Fragments arranged by size:
4090 3637 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 3108 3213 3228 3861 5932 1353
Size: 1488 267 105 15 633 2071 3423
Fragments arranged by size:
3423 2071 1488 633 267 105 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3867 6589 6963 7109 387
Size: 1064 138 2278 2722 374 146 1280
Fragments arranged by size:
2722 2278 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4654 4654
Size: 8002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2124 3741 3786 3861 3956 3975
Size: 393 455 37 1617 45 75 95 19
Cuts at: 3975 4334 4971 5249 6065 6662 7449 7717 1239
Size: 359 637 278 816 597 787 268 1524
Fragments arranged by size:
1617 1524 816 787 637 597 455 393
359 278 268 95 75 45 37 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2545 2051
Size: 494 7508
EcoO109I rG'GnC_Cy
Cuts at: 2134 3238 4079 4480 2134
Size: 1104 841 401 5656
Fragments arranged by size:
5656 1104 841 401
EcoRI G'AATT_C
Cuts at: 4487 5311 5689 4487
Size: 824 378 6800
Fragments arranged by size:
6800 824 378
FspI TGC'GCA
Cuts at: 6508 6508
Size: 8002
HaeII r_GCGC'y
Cuts at: 1678 2436 7149 1678
Size: 758 4713 2531
Fragments arranged by size:
4713 2531 758
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3135 3167 3716 3748 4511
Size: 32 135 32 1563 32 549 32 763
Cuts at: 4511 4543 4643 4675 4683 4715 4999 5031 5051
Size: 32 100 32 8 32 284 32 20
Cuts at: 5051 5083 6643 6675 6717 6749 1373
Size: 32 1560 32 42 32 2626
Fragments arranged by size:
2626 1563 1560 763 549 284 135 100
42 32 32 32 32 32 32 32
32 32 32 32 20 8
HincII GTy'rAC
Cuts at: 669 1657 1938 5346 5592 669
Size: 988 281 3408 246 3079
Fragments arranged by size:
3408 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2883 748
Size: 2135 5867
HpaI GTT'AAC
Cuts at: 5592 5592
Size: 8002
KpnI G_GTAC'C
Cuts at: 5327 5327
Size: 8002
MfeI C'AATT_G
Cuts at: 2396 5601 2396
Size: 3205 4797
MluI A'CGCG_T
Cuts at: 7878 7878
Size: 8002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2984 4958 7116 7300 1305
Size: 1679 1974 2158 184 2007
Fragments arranged by size:
2158 2007 1974 1679 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6180
Fragments arranged by size:
6180 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 2796 2946 3228 5960 6319 6478
Size: 648 1497 133 150 282 2732 359 159
Cuts at: 6478 518
Size: 2042
Fragments arranged by size:
2732 2042 1497 648 359 282 159 150
133
MspA1I CmG'CkG
Cuts at: 1606 2102 2684 3944 4159 4282 5236 5418 6095
Size: 496 582 1260 215 123 954 182 677
Cuts at: 6095 7243 7488 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1260 1148 954 677 582 496 245
215 182 123
NaeI GCC'GGC
Cuts at: 4212 4212
Size: 8002
NcoI C'CATG_G
Cuts at: 513 1065 3123 4443 5040 5134 5145 513
Size: 552 2058 1320 597 94 11 3370
Fragments arranged by size:
3370 2058 1320 597 552 94 11
NdeI CA'TA_TG
Cuts at: 387 3889 387
Size: 3502 4500
NgoMIV G'CCGG_C
Cuts at: 4210 4210
Size: 8002
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8002
NotI GC'GGCC_GC
Cuts at: 5449 5449
Size: 8002
NsiI A_TGCA'T
Cuts at: 5211 5211
Size: 8002
NspI r_CATG'y
Cuts at: 1132 1229 2555 5360 6905 1132
Size: 97 1326 2805 1545 2229
Fragments arranged by size:
2805 2229 1545 1326 97
PasI CC'CwG_GG
Cuts at: 1348 3147 3584 1348
Size: 1799 437 5766
Fragments arranged by size:
5766 1799 437
PciI A'CATG_T
Cuts at: 6901 6901
Size: 8002
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3129 1108
Size: 2021 5981
PfoI T'CCnGG_A
Cuts at: 5212 5212
Size: 8002
PmeI GTTT'AAAC
Cuts at: 5306 5306
Size: 8002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2184 2216 6071 6103 7584 7616 2184
Size: 32 3855 32 1481 32 2570
Fragments arranged by size:
3855 2570 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 3238 4079 4480 2134
Size: 1104 841 401 5656
Fragments arranged by size:
5656 1104 841 401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8002
PsiI TTA'TAA
Cuts at: 2589 5572 2589
Size: 2983 5019
PstI C_TGCA'G
Cuts at: 830 2034 2133 2654 3574 4164 5014 5354 830
Size: 1204 99 521 920 590 850 340 3478
Fragments arranged by size:
3478 1204 920 850 590 521 340 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8002
PvuII CAG'CTG
Cuts at: 2102 2684 3944 4159 4282 5236 2102
Size: 582 1260 215 123 954 4868
Fragments arranged by size:
4868 1260 954 582 215 123
SacI G_AGCT'C
Cuts at: 4656 4656
Size: 8002
SalI G'TCGA_C
Cuts at: 1936 5344 1936
Size: 3408 4594
SbfI CC_TGCA'GG
Cuts at: 5354 5354
Size: 8002
ScaI AGT'ACT
Cuts at: 6250 7962 6250
Size: 1712 6290
SexAI A'CCwGG_T
Cuts at: 4002 4002
Size: 8002
SfcI C'TryA_G
Cuts at: 826 1045 2030 2129 2545 2650 3062 3405 3570
Size: 219 985 99 416 105 412 343 165
Cuts at: 3570 3978 4160 4507 5010 5350 6485 7166 7357
Size: 408 182 347 503 340 1135 681 191
Cuts at: 7357 826
Size: 1471
Fragments arranged by size:
1471 1135 985 681 503 416 412 408
347 343 340 219 191 182 165 105
99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8002
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8002
SmaI CCC'GGG
Cuts at: 1872 5329 1872
Size: 3457 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3244 3801 6106 7007 7269 820
Size: 197 926 1301 557 2305 901 262 1553
Fragments arranged by size:
2305 1553 1301 926 901 557 262 197
SnaBI TAC'GTA
Cuts at: 493 5299 5793 493
Size: 4806 494 2702
Fragments arranged by size:
4806 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8002
SphI G_CATG'C
Cuts at: 1132 2555 5360 1132
Size: 1423 2805 3774
Fragments arranged by size:
3774 2805 1423
SspI AAT'ATT
Cuts at: 5 52 5926 5
Size: 47 5874 2081
Fragments arranged by size:
5874 2081 47
StuI AGG'CCT
Cuts at: 1517 2777 1517
Size: 1260 6742
StyI C'CwwG_G
Cuts at: 513 1065 2494 3123 3434 3700 4443 5040 5134
Size: 552 1429 629 311 266 743 597 94
Cuts at: 5134 5145 5441 513
Size: 11 296 3074
Fragments arranged by size:
3074 1429 743 629 597 552 311 296
266 94 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5033 6029 6046 6199 6384 6829 325
Size: 1255 3453 996 17 153 185 445 1498
Fragments arranged by size:
3453 1498 1255 996 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2351 3183 6248
Size: 274 80 33 51 1443 374 832 3065
Cuts at: 6248 7960 96
Size: 1712 138
Fragments arranged by size:
3065 1712 1443 832 374 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3990 4022 7962 7994 3990
Size: 32 3940 32 3998
Fragments arranged by size:
3998 3940 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8002
XbaI T'CTAG_A
Cuts at: 4417 5338 4417
Size: 921 7081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2106 4010 4491 2106
Size: 1904 481 5617
Fragments arranged by size:
5617 1904 481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8002
XmaI C'CCGG_G
Cuts at: 1870 5327 1870
Size: 3457 4545
XmnI GAAnn'nnTTC
Cuts at: 3218 6131 3218
Size: 2913 5089
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5810 276
Size: 53 83 186 936 4276 2468
Fragments arranged by size:
4276 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NarI NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI