Restriction map for
pFN21ASDA0248
(Circular) (Six-base) MAPSORT of: pFN21ASDA0248.seq Check: 8410 from: 1 to: 10306
pFN21ASDA0248 10306 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7465 7465
Size: 10306
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8116 278
Size: 53 83 186 936 6580 2468
Fragments arranged by size:
6580 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 5988 7649 1937
Size: 643 3408 1661 4594
Fragments arranged by size:
4594 3408 1661 643
Acc65I G'GTAC_C
Cuts at: 7627 7627
Size: 10306
AclI AA'CG_TT
Cuts at: 1563 8433 8806 1563
Size: 6870 373 3063
Fragments arranged by size:
6870 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2417 3105 4318 4879 5792 7055 8369
Size: 633 152 688 1213 561 913 1263 1314
Cuts at: 8369 9753 10021 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1314 1263 1213 913 688 633
561 268 152
AflII C'TTAA_G
Cuts at: 820 1017 6191 820
Size: 197 5174 4935
Fragments arranged by size:
5174 4935 197
AflIII A'CryG_T
Cuts at: 1203 4644 9205 10182 1203
Size: 3441 4561 977 1327
Fragments arranged by size:
4561 3441 1327 977
AgeI A'CCGG_T
Cuts at: 9137 9137
Size: 10306
AhdI GACnn_n'nnGTC
Cuts at: 6181 7009 9035 6181
Size: 828 2026 7452
Fragments arranged by size:
7452 2026 828
AleI CACnn'nnGTG
Cuts at: 1166 5577 5799 1166
Size: 4411 222 5673
Fragments arranged by size:
5673 4411 222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4781 6684 1184
Size: 3597 1903 4806
Fragments arranged by size:
4806 3597 1903
AlwNI CAG_nnn'CTG
Cuts at: 2366 2864 2990 3068 3347 4183 4348 5683 6715
Size: 498 126 78 279 836 165 1335 1032
Cuts at: 6715 6913 8049 9621 2366
Size: 198 1136 1572 3051
Fragments arranged by size:
3051 1572 1335 1136 1032 836 498 279
198 165 126 78
ApaI G_GGCC'C
Cuts at: 4820 5309 5911 6105 6640 4820
Size: 489 602 194 535 8486
Fragments arranged by size:
8486 602 535 489 194
ApaLI G'TGCA_C
Cuts at: 3100 8363 9519 3100
Size: 5263 1156 3887
Fragments arranged by size:
5263 3887 1156
ApoI r'AATT_y
Cuts at: 1493 2089 2906 4970 7615 7841 7993 10102 1493
Size: 596 817 2064 2645 226 152 2109 1697
Fragments arranged by size:
2645 2109 2064 1697 817 596 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8860 160
Size: 872 7828 1606
Fragments arranged by size:
7828 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10306
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 3162 3879 5730 6432 7334 7344
Size: 73 1071 148 717 1851 702 902 10
Cuts at: 7344 7631 1870
Size: 287 4545
Fragments arranged by size:
4545 1851 1071 902 717 702 287 148
73 10
BamHI G'GATC_C
Cuts at: 2131 2395 5185 7636 8003 2131
Size: 264 2790 2451 367 4434
Fragments arranged by size:
4434 2790 2451 367 264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2468 3875 4742 6648 6693
Size: 325 217 627 681 1407 867 1906 45
Cuts at: 6693 7627 9082 618
Size: 934 1455 1842
Fragments arranged by size:
1906 1842 1455 1407 934 867 681 627
325 217 45
BanII G_rGCy'C
Cuts at: 1391 2205 3243 3636 4820 5143 5309 5424 5760
Size: 814 1038 393 1184 323 166 115 336
Cuts at: 5760 5911 5928 6105 6261 6640 7343 7406 7625
Size: 151 17 177 156 379 703 63 219
Cuts at: 7625 1391
Size: 4072
Fragments arranged by size:
4072 1184 1038 814 703 393 379 336
323 219 177 166 156 151 115 63
17
BbeI G_GCGC'C
Cuts at: 6697 6697
Size: 10306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2940 3215 5780 6892 928
Size: 971 1041 275 2565 1112 4342
Fragments arranged by size:
4342 2565 1112 1041 971 275
BbvCI CC'TCA_GC
Cuts at: 2869 4931 7234 2869
Size: 2062 2303 5941
Fragments arranged by size:
5941 2303 2062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5537 5571 8497 8531 1903
Size: 34 3600 34 2926 34 3678
Fragments arranged by size:
3678 3600 2926 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8198 9408 8198
Size: 1210 9096
BclI T'GATC_A
Cuts at: 1548 5379 6129 1548
Size: 3831 750 5725
Fragments arranged by size:
5725 3831 750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6114 8917 136
Size: 107 122 71 631 605 4442 2803 1525
Fragments arranged by size:
4442 2803 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5199 10301 5199
Size: 5102 5204
BlpI GC'TnA_GC
Cuts at: 5252 7400 7434 7723 5252
Size: 2148 34 289 7835
Fragments arranged by size:
7835 2148 289 34
Bme1580I G_kGCm'C
Cuts at: 1790 2336 2393 2473 2835 3104 4820 5309 5911
Size: 546 57 80 362 269 1716 489 602
Cuts at: 5911 6105 6640 6653 7548 8367 9523 1790
Size: 194 535 13 895 819 1156 2573
Fragments arranged by size:
2573 1716 1156 895 819 602 546 535
489 362 269 194 80 57 13
BmgBI CAC'GTC
Cuts at: 1318 3105 5950 1318
Size: 1787 2845 5674
Fragments arranged by size:
5674 2845 1787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2456 3052 3349
Size: 416 53 83 399 24 1039 596 297
Cuts at: 3349 5004 5712 5818 5905 8037 8995 442
Size: 1655 708 106 87 2132 958 1753
Fragments arranged by size:
2132 1753 1655 1039 958 708 596 416
399 297 106 87 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3891 1056
Size: 2835 7471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 3509 7184 7552 2739
Size: 770 3675 368 5493
Fragments arranged by size:
5493 3675 770 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2949 3014 3608 4034 4448 4595 4658 5384
Size: 1710 65 594 426 414 147 63 726
Cuts at: 5384 5426 5844 5852 6400 6611 6982 8966 1239
Size: 42 418 8 548 211 371 1984 2579
Fragments arranged by size:
2579 1984 1710 726 594 548 426 418
414 371 211 147 65 63 42 8
Bpu10I CC'TnA_GC
Cuts at: 2869 4931 5286 5504 5676 5845 7234 2869
Size: 2062 355 218 172 169 1389 5941
Fragments arranged by size:
5941 2062 1389 355 218 172 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3356 3479 4880 7197 7369 8431 9296 9594 3356
Size: 123 1401 2317 172 1062 865 298 4068
Fragments arranged by size:
4068 2317 1401 1062 865 298 172 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5443 5553 6857 8969 882
Size: 617 3944 110 1304 2112 2219
Fragments arranged by size:
3944 2219 2112 1304 617 110
BsaAI yAC'GTr
Cuts at: 493 1204 7603 8097 493
Size: 711 6399 494 2702
Fragments arranged by size:
6399 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2426 3515 10300 2426
Size: 1089 6785 2432
Fragments arranged by size:
6785 2432 1089
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6694 8113 8495 9130
Size: 53 83 186 936 5161 1419 382 635
Cuts at: 9130 275
Size: 1451
Fragments arranged by size:
5161 1451 1419 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2517 5655 6276 8739 9137 9411 9558 9969 2517
Size: 3138 621 2463 398 274 147 411 2854
Fragments arranged by size:
3138 2854 2463 621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3089 3119 4269 4299 4631
Size: 30 165 30 1686 30 1150 30 332
Cuts at: 4631 4661 6980 7010 1178
Size: 30 2319 30 4474
Fragments arranged by size:
4474 2319 1686 1150 332 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2836 2902 3110 4190 4592 4700 4877 5250
Size: 1650 66 208 1080 402 108 177 373
Cuts at: 5250 6590 6875 1186
Size: 1340 285 4617
Fragments arranged by size:
4617 1650 1340 1080 402 373 285 208
177 108 66
BseYI C'CCAG_C
Cuts at: 3617 3825 6104 7309 7391 9509 10005 3617
Size: 208 2279 1205 82 2118 496 3918
Fragments arranged by size:
3918 2279 2118 1205 496 208 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 3620 6521 7101 1163
Size: 573 873 1011 2901 580 4368
Fragments arranged by size:
4368 2901 1011 873 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 7756 8517 9545 1540
Size: 275 186 1279 4476 761 1028 2301
Fragments arranged by size:
4476 2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2571 3104 3243 3492 3636 4331 5424 5677
Size: 466 533 139 249 144 695 1093 253
Cuts at: 5677 5928 6261 6736 8367 8452 9523 2105
Size: 251 333 475 1631 85 1071 2888
Fragments arranged by size:
2888 1631 1093 1071 695 533 475 466
333 253 251 249 144 139 85
BsmI GAATG_Cn'
Cuts at: 3712 4433 4564 7817 7910 3712
Size: 721 131 3253 93 6108
Fragments arranged by size:
6108 3253 721 131 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2205 2336 2393 2473 2571 2835
Size: 399 315 100 131 57 80 98 264
Cuts at: 2835 3104 3243 3492 3636 4331 4820 5143 5309
Size: 269 139 249 144 695 489 323 166
Cuts at: 5309 5424 5677 5760 5911 5928 6105 6261 6640
Size: 115 253 83 151 17 177 156 379
Cuts at: 6640 6653 6736 7343 7406 7548 7625 8367 8452
Size: 13 83 607 63 142 77 742 85
Cuts at: 8452 9523 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 695 607 489 399 379
323 315 269 264 253 249 177 166
156 151 144 142 139 131 115 100
98 85 83 83 80 77 63 57
17 13
BspEI T'CCGG_A
Cuts at: 2517 5655 6276 9969 2517
Size: 3138 621 3693 2854
Fragments arranged by size:
3693 3138 2854 621
BspHI T'CATG_A
Cuts at: 5424 8195 9925 5424
Size: 2771 1730 5805
Fragments arranged by size:
5805 2771 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5013 7465 7661 844
Size: 4169 2452 196 3489
Fragments arranged by size:
4169 3489 2452 196
BsrBI CCG'CTC
Cuts at: 1394 1823 5614 8193 1394
Size: 429 3791 2579 3507
Fragments arranged by size:
3791 3507 2579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5366 6884 8801 8975 65
Size: 1183 4118 1518 1917 174 1396
Fragments arranged by size:
4118 1917 1518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6402 8950 9137 1700
Size: 110 4592 2548 187 2869
Fragments arranged by size:
4592 2869 2548 187 110
BsrGI T'GTAC_A
Cuts at: 96 3513 6742 96
Size: 3417 3229 3660
Fragments arranged by size:
3660 3417 3229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10306
BssSI C'ACGA_G
Cuts at: 8366 9378 8366
Size: 1012 9294
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5900 6535 794
Size: 5106 635 4565
Fragments arranged by size:
5106 4565 635
BstBI TT'CG_AA
Cuts at: 2007 9110 2007
Size: 7103 3203
BstEII G'GTnAC_C
Cuts at: 1184 2607 1184
Size: 1423 8883
BstXI CCAn_nnnn'nTGG
Cuts at: 4213 4573 5078 5338 6823 7204 4213
Size: 360 505 260 1485 381 7315
Fragments arranged by size:
7315 1485 505 381 360 260
BstYI r'GATC_y
Cuts at: 1972 2131 2317 2395 2520 2717 4087 5185 5199
Size: 159 186 78 125 197 1370 1098 14
Cuts at: 5199 6279 7573 7636 8003 8388 8405 9857 9943
Size: 1080 1294 63 367 385 17 1452 86
Cuts at: 9943 9955 10301 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1370 1294 1098 1080 385 367
346 197 186 159 125 86 78 63
17 14 12
Bsu36I CC'TnA_GG
Cuts at: 4683 4752 4683
Size: 69 10237
BtgI C'CryG_G
Cuts at: 513 1065 2022 4091 5998 7203 513
Size: 552 957 2069 1907 1205 3616
Fragments arranged by size:
3616 2069 1907 1205 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3927 4497 4950 6699 487
Size: 683 56 2701 570 453 1749 4094
Fragments arranged by size:
4094 2701 1749 683 570 453 56
BtsI GCAGTG_nn'
Cuts at: 2880 3004 3793 4012 4993 5625 5825 6640 6920
Size: 124 789 219 981 632 200 815 280
Cuts at: 6920 7469 7822 8616 8636 2880
Size: 549 353 794 20 4550
Fragments arranged by size:
4550 981 815 794 789 632 549 353
280 219 200 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10271
DraI TTT'AAA
Cuts at: 2172 2620 7610 2172
Size: 448 4990 4868
Fragments arranged by size:
4990 4868 448
DraIII CAC_nnn'GTG
Cuts at: 5279 5279
Size: 10306
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5984 9313 809
Size: 507 4668 3329 1802
Fragments arranged by size:
4668 3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5640 6601 7276
Size: 54 1425 50 275 18 3810 961 675
Cuts at: 7276 7554 7753 8642 10271 8
Size: 278 199 889 1629 43
Fragments arranged by size:
3810 1629 1425 961 889 675 278 275
199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7753 1537
Size: 275 5941 4090
Fragments arranged by size:
5941 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 2708 3248 4805 5138 5211 5451 5658
Size: 1250 105 540 1557 333 73 240 207
Cuts at: 5658 5825 7175 8236 1353
Size: 167 1350 1061 3423
Fragments arranged by size:
3423 1557 1350 1250 1061 540 333 240
207 167 105 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4358 4906 6321 6587 8893 9267
Size: 1064 138 2769 548 1415 266 2306 374
Cuts at: 9267 9413 387
Size: 146 1280
Fragments arranged by size:
2769 2306 1415 1280 1064 548 374 266
146 138
EcoICRI GAG'CTC
Cuts at: 3241 3634 5422 5926 6259 3241
Size: 393 1788 504 333 7288
Fragments arranged by size:
7288 1788 504 393 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2417 2949 3014 3105 3608 4034
Size: 393 633 152 532 65 91 503 426
Cuts at: 4034 4318 4448 4595 4658 4879 5384 5426 5792
Size: 284 130 147 63 221 505 42 366
Cuts at: 5792 5844 5852 6400 6611 6982 7055 8369 8966
Size: 52 8 548 211 371 73 1314 597
Cuts at: 8966 9753 10021 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1314 787 633 597 548 532 505
503 426 393 371 366 284 268 221
211 152 147 130 91 73 65 63
52 42 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3380 3677 5093 3380
Size: 297 1416 8593
Fragments arranged by size:
8593 1416 297
EcoO109I rG'GnC_Cy
Cuts at: 3039 4816 4817 5305 5907 6101 6116 7192 7314
Size: 1777 1 488 602 194 15 1076 122
Cuts at: 7314 3039
Size: 6031
Fragments arranged by size:
6031 1777 1076 602 488 194 122 15
1
EcoRI G'AATT_C
Cuts at: 2906 7615 7993 2906
Size: 4709 378 5219
Fragments arranged by size:
5219 4709 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3727 3727
Size: 10306
FspI TGC'GCA
Cuts at: 5696 8812 5696
Size: 3116 7190
HaeII r_GCGC'y
Cuts at: 1678 6697 9453 1678
Size: 5019 2756 2531
Fragments arranged by size:
5019 2756 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2262 2294 3089 3116 3121
Size: 32 135 32 690 32 795 27 5
Cuts at: 3121 3148 3382 3414 5210 5242 5652 5659 5684
Size: 27 234 32 1796 32 410 7 25
Cuts at: 5684 5691 5915 5944 5947 5976 6167 6199 6995
Size: 7 224 29 3 29 191 32 796
Cuts at: 6995 7027 7286 7318 8947 8979 9021 9053 1373
Size: 32 259 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1796 1629 796 795 690 410 259
234 224 191 135 42 32 32 32
32 32 32 32 32 32 32 29
29 27 27 25 7 7 5 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 3728 4027 5244 6049 7597
Size: 988 281 352 1438 299 1217 805 1548
Cuts at: 7597 7650 7896 669
Size: 53 246 3079
Fragments arranged by size:
3079 1548 1438 1217 988 805 352 299
281 246 53
HindIII A'AGCT_T
Cuts at: 748 3524 748
Size: 2776 7530
HpaI GTT'AAC
Cuts at: 7896 7896
Size: 10306
KasI G'GCGC_C
Cuts at: 6693 6693
Size: 10306
KpnI G_GTAC'C
Cuts at: 7631 7631
Size: 10306
MfeI C'AATT_G
Cuts at: 7905 7905
Size: 10306
MluI A'CGCG_T
Cuts at: 10182 10182
Size: 10306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3693 4293 4427 5496 6000 9420 9604 1305
Size: 2388 600 134 1069 504 3420 184 2007
Fragments arranged by size:
3420 2388 2007 1069 600 504 184 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5642 6603 7278 7556 10
Size: 54 1425 343 3810 961 675 278 2760
Fragments arranged by size:
3810 2760 1425 961 675 343 278 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2698 2828 3542 4211 4740 5076 5577
Size: 648 1532 130 714 669 529 336 501
Cuts at: 5577 5799 5943 6345 7202 8264 8623 8782 518
Size: 222 144 402 857 1062 359 159 2042
Fragments arranged by size:
2042 1532 1062 857 714 669 648 529
501 402 359 336 222 159 144 130
MspA1I CmG'CkG
Cuts at: 1606 2593 5212 5404 5864 7064 7253 7722 8399
Size: 987 2619 192 460 1200 189 469 677
Cuts at: 8399 9547 9792 1606
Size: 1148 245 2120
Fragments arranged by size:
2619 2120 1200 1148 987 677 469 460
245 192 189
NaeI GCC'GGC
Cuts at: 6404 6404
Size: 10306
NarI GG'CG_CC
Cuts at: 6694 6694
Size: 10306
NcoI C'CATG_G
Cuts at: 513 1065 2022 5998 7203 513
Size: 552 957 3976 1205 3616
Fragments arranged by size:
3976 3616 1205 957 552
NdeI CA'TA_TG
Cuts at: 387 2739 5464 387
Size: 2352 2725 5229
Fragments arranged by size:
5229 2725 2352
NgoMIV G'CCGG_C
Cuts at: 6402 6402
Size: 10306
NheI G'CTAG_C
Cuts at: 1052 3887 1052
Size: 2835 7471
NotI GC'GGCC_GC
Cuts at: 7753 7753
Size: 10306
NspI r_CATG'y
Cuts at: 1132 1229 2818 3484 4648 6428 6988 7045 7664
Size: 97 1589 666 1164 1780 560 57 619
Cuts at: 7664 9209 1132
Size: 1545 2229
Fragments arranged by size:
2229 1780 1589 1545 1164 666 619 560
97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10306
PciI A'CATG_T
Cuts at: 4644 9205 4644
Size: 4561 5745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3145 3989 4812 1108
Size: 2037 844 823 6602
Fragments arranged by size:
6602 2037 844 823
PfoI T'CCnGG_A
Cuts at: 3030 4635 7188 3030
Size: 1605 2553 6148
Fragments arranged by size:
6148 2553 1605
PmeI GTTT'AAAC
Cuts at: 7610 7610
Size: 10306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8375 8407 9888 9920 8375
Size: 32 1481 32 8761
Fragments arranged by size:
8761 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3039 7192 3039
Size: 4153 6153
PshAI GACnn'nnGTC
Cuts at: 1613 2747 1613
Size: 1134 9172
PsiI TTA'TAA
Cuts at: 7876 7876
Size: 10306
PspOMI G'GGCC_C
Cuts at: 4816 5305 5907 6101 6636 4816
Size: 489 602 194 535 8486
Fragments arranged by size:
8486 602 535 489 194
PstI C_TGCA'G
Cuts at: 830 5130 5874 6663 6729 6844 6909 7455 7476
Size: 4300 744 789 66 115 65 546 21
Cuts at: 7476 7658 830
Size: 182 3478
Fragments arranged by size:
4300 3478 789 744 546 182 115 66
65 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10306
PvuII CAG'CTG
Cuts at: 2593 5404 5864 2593
Size: 2811 460 7035
Fragments arranged by size:
7035 2811 460
SacI G_AGCT'C
Cuts at: 3243 3636 5424 5928 6261 3243
Size: 393 1788 504 333 7288
Fragments arranged by size:
7288 1788 504 393 333
SalI G'TCGA_C
Cuts at: 1936 7648 1936
Size: 5712 4594
SanDI GG'GwC_CC
Cuts at: 3039 3039
Size: 10306
SapI GCTCTTCn'nnn_
Cuts at: 2603 3248 5138 2603
Size: 645 1890 7771
Fragments arranged by size:
7771 1890 645
SbfI CC_TGCA'GG
Cuts at: 7658 7658
Size: 10306
ScaI AGT'ACT
Cuts at: 8554 10266 8554
Size: 1712 8594
SexAI A'CCwGG_T
Cuts at: 2755 5742 2755
Size: 2987 7319
SfcI C'TryA_G
Cuts at: 826 1045 2889 2982 3348 3924 5126 5207 5414
Size: 219 1844 93 366 576 1202 81 207
Cuts at: 5414 5870 6559 6659 6725 6840 6905 7451 7472
Size: 456 689 100 66 115 65 546 21
Cuts at: 7472 7654 8789 9470 9661 826
Size: 182 1135 681 191 1471
Fragments arranged by size:
1844 1471 1202 1135 689 681 576 546
456 366 219 207 191 182 115 100
93 81 66 65 21
SfoI GGC'GCC
Cuts at: 6695 6695
Size: 10306
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10306
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10306
SmaI CCC'GGG
Cuts at: 1872 3164 7633 1872
Size: 1292 4469 4545
Fragments arranged by size:
4545 4469 1292
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3335 3494 4859 6191 7212 7384
Size: 197 926 1392 159 1365 1332 1021 172
Cuts at: 7384 8410 9311 9573 820
Size: 1026 901 262 1553
Fragments arranged by size:
1553 1392 1365 1332 1026 1021 926 901
262 197 172 159
SnaBI TAC'GTA
Cuts at: 493 7603 8097 493
Size: 7110 494 2702
Fragments arranged by size:
7110 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10306
SphI G_CATG'C
Cuts at: 1132 2818 3484 7664 1132
Size: 1686 666 4180 3774
Fragments arranged by size:
4180 3774 1686 666
SspI AAT'ATT
Cuts at: 5 52 2038 8230 5
Size: 47 1986 6192 2081
Fragments arranged by size:
6192 2081 1986 47
StuI AGG'CCT
Cuts at: 1517 3951 4197 1517
Size: 2434 246 7626
Fragments arranged by size:
7626 2434 246
StyI C'CwwG_G
Cuts at: 513 1065 2022 2928 5154 5998 7203 7428 7458
Size: 552 957 906 2226 844 1205 225 30
Cuts at: 7458 7745 513
Size: 287 3074
Fragments arranged by size:
3074 2226 1205 957 906 844 552 287
225 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 2141 2339 2387 2456 3839 4579
Size: 1255 540 21 198 48 69 1383 740
Cuts at: 4579 6839 8333 8350 8503 8688 9133 325
Size: 2260 1494 17 153 185 445 1498
Fragments arranged by size:
2260 1498 1494 1383 1255 740 540 445
198 185 153 69 48 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3513 6742 6981
Size: 274 80 33 51 1443 1536 3229 239
Cuts at: 6981 8552 10264 96
Size: 1571 1712 138
Fragments arranged by size:
3229 1712 1571 1536 1443 274 239 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 4269 4301 4398 4430 6980 7012 7232
Size: 32 860 32 97 32 2550 32 220
Cuts at: 7232 7264 7362 7394 10266 10298 3377
Size: 32 98 32 2872 32 3385
Fragments arranged by size:
3385 2872 2550 860 220 98 97 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10306
XbaI T'CTAG_A
Cuts at: 2947 7642 2947
Size: 4695 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 5750 6326 2638
Size: 3112 576 6618
Fragments arranged by size:
6618 3112 576
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10306
XmaI C'CCGG_G
Cuts at: 1870 3162 7631 1870
Size: 1292 4469 4545
Fragments arranged by size:
4545 4469 1292
XmnI GAAnn'nnTTC
Cuts at: 2235 3710 6365 8435 2235
Size: 1475 2655 2070 4106
Fragments arranged by size:
4106 2655 2070 1475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8114 276
Size: 53 83 186 936 6580 2468
Fragments arranged by size:
6580 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SfiI SrfI SwaI