Restriction map for
pFN21ASDA0245
(Circular) (Six-base) MAPSORT of: pFN21ASDA0245.seq Check: 9534 from: 1 to: 6274
pFN21ASDA0245 6274 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3595 3595
Size: 6274
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 3184 3190 4337 5721 5989 1632
Size: 533 1019 6 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 1019 533 268 6
AfeI AGC'GCT
Cuts at: 2628 2628
Size: 6274
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 2588 2590 2601 5173 6150 1203
Size: 1385 2 11 2572 977 1327
Fragments arranged by size:
2572 1385 1327 977 11 2
AgeI A'CCGG_T
Cuts at: 2435 5105 2435
Size: 2670 3604
AhdI GACnn_n'nnGTC
Cuts at: 3088 5003 3088
Size: 1915 4359
AleI CACnn'nnGTG
Cuts at: 1166 2591 1166
Size: 1425 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2564 2609 1184
Size: 1380 45 4849
Fragments arranged by size:
4849 1380 45
AlwNI CAG_nnn'CTG
Cuts at: 3213 4017 5589 3213
Size: 804 1572 3898
Fragments arranged by size:
3898 1572 804
ApaLI G'TGCA_C
Cuts at: 4331 5487 4331
Size: 1156 5118
ApoI r'AATT_y
Cuts at: 1493 2818 3583 3809 3961 6070 1493
Size: 1325 765 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 765 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3369 4828 160
Size: 872 2337 1459 1606
Fragments arranged by size:
2337 1606 1459 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 3063 3599 1870
Size: 73 1120 536 4545
Fragments arranged by size:
4545 1120 536 73
BamHI G'GATC_C
Cuts at: 3604 3971 3604
Size: 367 5907
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3491 3595 5050 618
Size: 325 217 627 516 1188 104 1455 1842
Fragments arranged by size:
1842 1455 1188 627 516 325 217 104
BanII G_rGCy'C
Cuts at: 1391 2289 2433 3062 3270 3593 1391
Size: 898 144 629 208 323 4072
Fragments arranged by size:
4072 898 629 323 208 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2179 2213 4465 4499 1903
Size: 34 242 34 2252 34 3678
Fragments arranged by size:
3678 2252 242 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6274
BfrBI ATG'CAT
Cuts at: 3023 3023
Size: 6274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2044 3274 4885
Size: 107 122 71 631 605 372 1230 1611
Cuts at: 4885 136
Size: 1525
Fragments arranged by size:
1611 1525 1230 631 605 372 122 107
71
BglII A'GATC_T
Cuts at: 2154 6269 2154
Size: 4115 2159
BlpI GC'TnA_GC
Cuts at: 3293 3691 3293
Size: 398 5876
Bme1580I G_kGCm'C
Cuts at: 1790 2308 2588 3351 4335 5491 1790
Size: 518 280 763 984 1156 2573
Fragments arranged by size:
2573 1156 984 763 518 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4005 4963 442
Size: 416 53 83 399 24 2588 958 1753
Fragments arranged by size:
2588 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3423 4934 1239
Size: 2184 1511 2579
Fragments arranged by size:
2579 2184 1511
Bpu10I CC'TnA_GC
Cuts at: 2203 2918 2203
Size: 715 5559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3272 4399 5264 5562 3272
Size: 1127 865 298 3984
Fragments arranged by size:
3984 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2416 4937 882
Size: 617 917 2521 2219
Fragments arranged by size:
2521 2219 917 617
BsaAI yAC'GTr
Cuts at: 493 1204 2591 3571 4065 493
Size: 711 1387 980 494 2702
Fragments arranged by size:
2702 1387 980 711 494
BsaBI GATnn'nnATC
Cuts at: 6268 6268
Size: 6274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2694 4081 4463 5098
Size: 53 83 186 936 1161 1387 382 635
Cuts at: 5098 275
Size: 1451
Fragments arranged by size:
1451 1387 1161 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2435 3079 4707 5105 5379 5526 5937 2435
Size: 644 1628 398 274 147 411 2772
Fragments arranged by size:
2772 1628 644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 1178
Size: 30 165 30 828 30 5191
Fragments arranged by size:
5191 828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6274
BseYI C'CCAG_C
Cuts at: 2323 2492 3495 5477 5973 2323
Size: 169 1003 1982 496 2624
Fragments arranged by size:
2624 1982 1003 496 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2615 1163
Size: 573 879 4822
Fragments arranged by size:
4822 879 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2685 4335 4420 5491 2685
Size: 1650 85 1071 3468
Fragments arranged by size:
3468 1650 1071 85
BsmI GAATG_Cn'
Cuts at: 3785 3878 3785
Size: 93 6181
BsmBI CGTCTCn'nnnn_
Cuts at: 2528 2528
Size: 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2308 2433 2588 2685 3062 3270
Size: 399 499 19 125 155 97 377 208
Cuts at: 3270 3351 3593 4335 4420 5491 1391
Size: 81 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 377 242 208
155 125 97 85 81 19
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3441 3629 844
Size: 2597 188 3489
Fragments arranged by size:
3489 2597 188
BsrBI CCG'CTC
Cuts at: 1394 1823 4161 1394
Size: 429 2338 3507
Fragments arranged by size:
3507 2338 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 2631 2985 4769 4943 65
Size: 1183 1140 243 354 1784 174 1396
Fragments arranged by size:
1784 1396 1183 1140 354 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2435 4918 5105 1700
Size: 110 625 2483 187 2869
Fragments arranged by size:
2869 2483 625 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6274
BstBI TT'CG_AA
Cuts at: 2007 5078 2007
Size: 3071 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstXI CCAn_nnnn'nTGG
Cuts at: 2960 2960
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 2154 2208 3604 3971 4356 4373 5825 5911
Size: 182 54 1396 367 385 17 1452 86
Cuts at: 5911 5923 6269 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1396 385 367 346 182 86
54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3147 513
Size: 552 957 1125 3640
Fragments arranged by size:
3640 1125 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2434 487
Size: 683 56 1208 4327
Fragments arranged by size:
4327 1208 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 2414 2929 3790 4584 4604 2241
Size: 173 515 861 794 20 3911
Fragments arranged by size:
3911 861 794 515 173 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 3578 3578
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2829 3721 4610
Size: 54 1425 50 275 18 999 892 889
Cuts at: 4610 6239 8
Size: 1629 43
Fragments arranged by size:
1629 1425 999 892 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 2759 3359 4204 1353
Size: 941 465 600 845 3423
Fragments arranged by size:
3423 941 845 600 465
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2390 4861 5235 5381 387
Size: 1064 138 801 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 801 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 3184 3190 3423 4337 4934 5721
Size: 393 533 1019 6 233 914 597 787
Cuts at: 5721 5989 1239
Size: 268 1524
Fragments arranged by size:
1524 1019 914 787 597 533 393 268
233 6
EcoO109I rG'GnC_Cy
Cuts at: 2124 3013 2124
Size: 889 5385
EcoRI G'AATT_C
Cuts at: 3583 3961 3583
Size: 378 5896
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 2630 5421 1678
Size: 952 2791 2531
Fragments arranged by size:
2791 2531 952
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 3074 3106 4915
Size: 32 135 32 521 32 949 32 1809
Cuts at: 4915 4947 4989 5021 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1809 949 521 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3618 3864 669
Size: 988 281 1680 246 3079
Fragments arranged by size:
3079 1680 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6274
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3599 3599
Size: 6274
MfeI C'AATT_G
Cuts at: 2850 3139 3873 2850
Size: 289 734 5251
Fragments arranged by size:
5251 734 289
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3220 5388 5572 1305
Size: 1915 2168 184 2007
Fragments arranged by size:
2168 2007 1915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2831 10
Size: 54 1425 343 999 3453
Fragments arranged by size:
3453 1425 999 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2645 4232 4591 4750 518
Size: 648 1425 54 1587 359 159 2042
Fragments arranged by size:
2042 1587 1425 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2143 2496 2973 3258 3690 4367 5515 5760
Size: 537 353 477 285 432 677 1148 245
Cuts at: 5760 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 537 477 432 353 285
245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3147 513
Size: 552 957 1125 3640
Fragments arranged by size:
3640 1125 957 552
NdeI CA'TA_TG
Cuts at: 387 2543 3323 387
Size: 2156 780 3338
Fragments arranged by size:
3338 2156 780
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6274
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NsiI A_TGCA'T
Cuts at: 3025 3025
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2968 3632 5177 1132
Size: 97 1739 664 1545 2229
Fragments arranged by size:
2229 1739 1545 664 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6274
PciI A'CATG_T
Cuts at: 5173 5173
Size: 6274
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6274
PfoI T'CCnGG_A
Cuts at: 3443 3443
Size: 6274
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PmlI CAC'GTG
Cuts at: 2591 2591
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2124 2124
Size: 6274
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PstI C_TGCA'G
Cuts at: 830 3626 830
Size: 2796 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 2143 2496 2973 2143
Size: 353 477 5444
Fragments arranged by size:
5444 477 353
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SapI GCTCTTCn'nnn_
Cuts at: 2294 2294
Size: 6274
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 4522 6234 4522
Size: 1712 4562
SfcI C'TryA_G
Cuts at: 826 1045 3622 4757 5438 5629 826
Size: 219 2577 1135 681 191 1471
Fragments arranged by size:
2577 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3065 3601 1872
Size: 1193 536 4545
Fragments arranged by size:
4545 1193 536
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3287 4378 5279 5541 820
Size: 197 926 1344 1091 901 262 1553
Fragments arranged by size:
1553 1344 1091 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3571 4065 493
Size: 3078 494 2702
Fragments arranged by size:
3078 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 3632 1132
Size: 2500 3774
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 1517
Size: 858 5416
StyI C'CwwG_G
Cuts at: 513 1065 2022 2223 2616 2826 3147 3713 513
Size: 552 957 201 393 210 321 566 3074
Fragments arranged by size:
3074 957 566 552 393 321 210 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2068 2236 2291 3413 3509 4301 4318
Size: 1255 488 168 55 1122 96 792 17
Cuts at: 4318 4471 4656 5101 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1122 792 488 445 185 168
153 96 55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4520 6232 96
Size: 274 80 33 51 1443 2543 1712 138
Fragments arranged by size:
2543 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 6234 6266 2231
Size: 32 3971 32 2239
Fragments arranged by size:
3971 2239 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6274
XbaI T'CTAG_A
Cuts at: 2368 3610 2368
Size: 1242 5032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2644 2230
Size: 414 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6274
XmaI C'CCGG_G
Cuts at: 1870 3063 3599 1870
Size: 1193 536 4545
Fragments arranged by size:
4545 1193 536
XmnI GAAnn'nnTTC
Cuts at: 4403 4403
Size: 6274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI