Restriction map for
pFN21ASDA0238
(Circular) (Six-base) MAPSORT of: pFN21ASDA0238.seq Check: 1416 from: 1 to: 6679
pFN21ASDA0238 6679 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4489 278
Size: 53 83 186 936 2953 2468
Fragments arranged by size:
2953 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4022 1937
Size: 2085 4594
Acc65I G'GTAC_C
Cuts at: 2319 4000 2319
Size: 1681 4998
AclI AA'CG_TT
Cuts at: 1563 2492 4806 5179 1563
Size: 929 2314 373 3063
Fragments arranged by size:
3063 2314 929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2347 2759 3853 4742 6126 6394 1632
Size: 715 412 1094 889 1384 268 1917
Fragments arranged by size:
1917 1384 1094 889 715 412 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6482
AflIII A'CryG_T
Cuts at: 1203 5578 6555 1203
Size: 4375 977 1327
Fragments arranged by size:
4375 1327 977
AgeI A'CCGG_T
Cuts at: 5510 5510
Size: 6679
AhdI GACnn_n'nnGTC
Cuts at: 5408 5408
Size: 6679
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2858 1184
Size: 1674 5005
AlwNI CAG_nnn'CTG
Cuts at: 4422 5994 4422
Size: 1572 5107
ApaLI G'TGCA_C
Cuts at: 3225 4736 5892 3225
Size: 1511 1156 4012
Fragments arranged by size:
4012 1511 1156
ApoI r'AATT_y
Cuts at: 1493 2894 3276 3556 3988 4214 4366 6475 1493
Size: 1401 382 280 432 226 152 2109 1697
Fragments arranged by size:
2109 1697 1401 432 382 280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5233 160
Size: 872 4201 1606
Fragments arranged by size:
4201 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
AvaI C'yCGr_G
Cuts at: 1870 1943 2550 4004 1870
Size: 73 607 1454 4545
Fragments arranged by size:
4545 1454 607 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2311 2344 2311
Size: 33 6646
BamHI G'GATC_C
Cuts at: 2655 3472 4009 4376 2655
Size: 817 537 367 4958
Fragments arranged by size:
4958 817 537 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2319 4000 5455 618
Size: 325 217 627 360 172 1681 1455 1842
Fragments arranged by size:
1842 1681 1455 627 360 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2190 2234 2734 3490 3606 3998 1391
Size: 799 44 500 756 116 392 4072
Fragments arranged by size:
4072 799 756 500 392 116 44
BbeI G_GCGC'C
Cuts at: 2151 2151
Size: 6679
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 3019 3960 928
Size: 971 201 919 941 3647
Fragments arranged by size:
3647 971 941 919 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2362 2396 4870 4904 1903
Size: 34 425 34 2474 34 3678
Fragments arranged by size:
3678 2474 425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2658 2998 3965 4571 5781 2658
Size: 340 967 606 1210 3556
Fragments arranged by size:
3556 1210 967 606 340
BclI T'GATC_A
Cuts at: 1548 3513 1548
Size: 1965 4714
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2718 5290 136
Size: 107 122 71 631 605 1046 2572 1525
Fragments arranged by size:
2572 1525 1046 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6674 6674
Size: 6679
BlpI GC'TnA_GC
Cuts at: 4096 4096
Size: 6679
Bme1580I G_kGCm'C
Cuts at: 1790 2385 3229 3244 4740 5896 1790
Size: 595 844 15 1496 1156 2573
Fragments arranged by size:
2573 1496 1156 844 595 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6679
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2839 3333 4410
Size: 416 53 83 399 24 1422 494 1077
Cuts at: 4410 5368 442
Size: 958 1753
Fragments arranged by size:
1753 1422 1077 958 494 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3431
Size: 6679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 3732 5339 1239
Size: 901 1592 1607 2579
Fragments arranged by size:
2579 1607 1592 901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4804 5669 5967 4804
Size: 865 298 5516
Fragments arranged by size:
5516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 5342 882
Size: 617 738 3105 2219
Fragments arranged by size:
3105 2219 738 617
BsaAI yAC'GTr
Cuts at: 493 1204 3976 4470 493
Size: 711 2772 494 2702
Fragments arranged by size:
2772 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6673 6673
Size: 6679
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 4486 4868 5503
Size: 53 83 186 936 615 2338 382 635
Cuts at: 5503 275
Size: 1451
Fragments arranged by size:
2338 1451 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2128 3469 3765 5112 5510 5784 5931 6342 2128
Size: 1341 296 1347 398 274 147 411 2465
Fragments arranged by size:
2465 1347 1341 411 398 296 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6454
Fragments arranged by size:
6454 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 1186
Size: 1519 5160
BseYI C'CCAG_C
Cuts at: 2110 2274 3481 5882 6378 2110
Size: 164 1207 2401 496 2411
Fragments arranged by size:
2411 2401 1207 496 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3481 1163
Size: 573 1745 4361
Fragments arranged by size:
4361 1745 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2376 3156 4129 4890 5918 1540
Size: 275 186 375 780 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 780 761 375 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2234 3229 4740 4825 5896 2190
Size: 44 995 1511 85 1071 2973
Fragments arranged by size:
2973 1511 1071 995 85 44
BsmI GAATG_Cn'
Cuts at: 4190 4283 4190
Size: 93 6586
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2234 2385 2734 3229 3244 3490
Size: 399 400 44 151 349 495 15 246
Cuts at: 3490 3606 3998 4740 4825 5896 1391
Size: 116 392 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 400 399 392 349
246 151 116 85 44 15
BspEI T'CCGG_A
Cuts at: 3469 3765 6342 3469
Size: 296 2577 3806
Fragments arranged by size:
3806 2577 296
BspHI T'CATG_A
Cuts at: 4568 6298 4568
Size: 1730 4949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2493 3546 4034 844
Size: 1649 1053 488 3489
Fragments arranged by size:
3489 1649 1053 488
BsrBI CCG'CTC
Cuts at: 1394 1823 4566 1394
Size: 429 2743 3507
Fragments arranged by size:
3507 2743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2217 2835 5174 5348 65
Size: 1183 969 618 2339 174 1396
Fragments arranged by size:
2339 1396 1183 969 618 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3155 5323 5510 1700
Size: 110 1345 2168 187 2869
Fragments arranged by size:
2869 2168 1345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6679
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6679
BssSI C'ACGA_G
Cuts at: 4739 5751 4739
Size: 1012 5667
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3085 794
Size: 2291 4388
BstBI TT'CG_AA
Cuts at: 2007 5483 2007
Size: 3476 3203
BstEII G'GTnAC_C
Cuts at: 1184 2505 1184
Size: 1321 5358
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 2410 2444 3129 2059
Size: 351 34 685 5609
Fragments arranged by size:
5609 685 351 34
BstYI r'GATC_y
Cuts at: 1972 2655 2664 3472 4009 4376 4761 4778 6230
Size: 683 9 808 537 367 385 17 1452
Cuts at: 6230 6316 6328 6674 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 808 683 537 385 367 346
86 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 3920 3920
Size: 6679
BtgI C'CryG_G
Cuts at: 513 1065 2769 3101 3180 3966 513
Size: 552 1704 332 79 786 3226
Fragments arranged by size:
3226 1704 786 552 332 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5940
Fragments arranged by size:
5940 683 56
BtsI GCAGTG_nn'
Cuts at: 2385 4195 4989 5009 2385
Size: 1810 794 20 4055
Fragments arranged by size:
4055 1810 794 20
ClaI AT'CG_AT
Cuts at: 1554 3660 1554
Size: 2106 4573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3879 3914 529
Size: 35 3315 35 3294
Fragments arranged by size:
3315 3294 35 35
DraI TTT'AAA
Cuts at: 3555 3983 3555
Size: 428 6251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5686 809
Size: 507 4370 1802
Fragments arranged by size:
4370 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2976 3153 4126
Size: 54 1425 50 275 18 1146 177 973
Cuts at: 4126 5015 6644 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1146 973 889 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3153 4126 1537
Size: 275 1341 973 4090
Fragments arranged by size:
4090 1341 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 3920 4609 1353
Size: 777 1790 689 3423
Fragments arranged by size:
3423 1790 777 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5266 5640 5786 387
Size: 1064 138 3677 374 146 1280
Fragments arranged by size:
3677 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 2232 2188
Size: 44 6635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2347 2759 3732 3853 4742 5339
Size: 393 508 207 412 973 121 889 597
Cuts at: 5339 6126 6394 1239
Size: 787 268 1524
Fragments arranged by size:
1524 973 889 787 597 508 412 393
268 207 121
EcoNI CCTnn'n_nnAGG
Cuts at: 2516 2516
Size: 6679
EcoRI G'AATT_C
Cuts at: 3988 4366 3988
Size: 378 6301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3104 3070
Size: 34 6645
FspI TGC'GCA
Cuts at: 5185 5185
Size: 6679
HaeII r_GCGC'y
Cuts at: 1678 2151 3453 5826 1678
Size: 473 1302 2373 2531
Fragments arranged by size:
2531 2373 1302 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3576 3608 3941 3973 5320
Size: 32 135 32 2004 32 333 32 1347
Cuts at: 5320 5352 5394 5426 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2004 1347 333 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4023 4269 669
Size: 988 281 2085 246 3079
Fragments arranged by size:
3079 2085 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6679
HpaI GTT'AAC
Cuts at: 4269 4269
Size: 6679
KasI G'GCGC_C
Cuts at: 2147 2147
Size: 6679
KpnI G_GTAC'C
Cuts at: 2323 4004 2323
Size: 1681 4998
MfeI C'AATT_G
Cuts at: 4278 4278
Size: 6679
MluI A'CGCG_T
Cuts at: 6555 6555
Size: 6679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5793 5977 1305
Size: 4488 184 2007
Fragments arranged by size:
4488 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2978 10
Size: 54 1425 343 1146 3711
Fragments arranged by size:
3711 1425 1146 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2681 2840 3127 3167 4637 4996
Size: 648 1242 273 159 287 40 1470 359
Cuts at: 4996 5155 518
Size: 159 2042
Fragments arranged by size:
2042 1470 1242 648 359 287 273 159
159 40
MspA1I CmG'CkG
Cuts at: 1606 2160 2367 2807 2950 3521 4095 4772 5920
Size: 554 207 440 143 571 574 677 1148
Cuts at: 5920 6165 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 574 571 554 440 245
207 143
NarI GG'CG_CC
Cuts at: 2148 2148
Size: 6679
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6127
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6679
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6679
NotI GC'GGCC_GC
Cuts at: 4126 4126
Size: 6679
NspI r_CATG'y
Cuts at: 1132 1229 2361 3172 4037 5582 1132
Size: 97 1132 811 865 1545 2229
Fragments arranged by size:
2229 1545 1132 865 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6679
PciI A'CATG_T
Cuts at: 5578 5578
Size: 6679
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6679
PfoI T'CCnGG_A
Cuts at: 3563 3723 3563
Size: 160 6519
PmeI GTTT'AAAC
Cuts at: 3983 3983
Size: 6679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4748 4780 6261 6293 4748
Size: 32 1481 32 5134
Fragments arranged by size:
5134 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3023 1613
Size: 1410 5269
PsiI TTA'TAA
Cuts at: 2913 3851 4249 2913
Size: 938 398 5343
Fragments arranged by size:
5343 938 398
PspXI vC'TCGA_Gb
Cuts at: 2550 2550
Size: 6679
PstI C_TGCA'G
Cuts at: 830 3543 4031 830
Size: 2713 488 3478
Fragments arranged by size:
3478 2713 488
PvuI CG_AT'CG
Cuts at: 2001 2376 2001
Size: 375 6304
PvuII CAG'CTG
Cuts at: 2950 3521 2950
Size: 571 6108
SacI G_AGCT'C
Cuts at: 2190 2234 2190
Size: 44 6635
SalI G'TCGA_C
Cuts at: 1936 4021 1936
Size: 2085 4594
SapI GCTCTTCn'nnn_
Cuts at: 3920 3920
Size: 6679
SbfI CC_TGCA'GG
Cuts at: 4031 4031
Size: 6679
ScaI AGT'ACT
Cuts at: 4927 6639 4927
Size: 1712 4967
SfcI C'TryA_G
Cuts at: 826 1045 2342 2483 3539 4027 5162 5843 6034
Size: 219 1297 141 1056 488 1135 681 191
Cuts at: 6034 826
Size: 1471
Fragments arranged by size:
1471 1297 1135 1056 681 488 219 191
141
SfiI GGCCn_nnn'nGGCC
Cuts at: 2718 2718
Size: 6679
SfoI GGC'GCC
Cuts at: 2149 2149
Size: 6679
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6679
SmaI CCC'GGG
Cuts at: 1872 4006 1872
Size: 2134 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2550 4783 5684 5946 820
Size: 197 926 607 2233 901 262 1553
Fragments arranged by size:
2233 1553 926 901 607 262 197
SnaBI TAC'GTA
Cuts at: 493 3976 4470 493
Size: 3483 494 2702
Fragments arranged by size:
3483 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6679
SphI G_CATG'C
Cuts at: 1132 3172 4037 1132
Size: 2040 865 3774
Fragments arranged by size:
3774 2040 865
SspI AAT'ATT
Cuts at: 5 52 2853 4603 5
Size: 47 2801 1750 2081
Fragments arranged by size:
2801 2081 1750 47
StuI AGG'CCT
Cuts at: 1517 3291 3918 1517
Size: 1774 627 4278
Fragments arranged by size:
4278 1774 627
StyI C'CwwG_G
Cuts at: 513 1065 4118 513
Size: 552 3053 3074
Fragments arranged by size:
3074 3053 552
SwaI ATTT'AAAT
Cuts at: 3555 3555
Size: 6679
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2124 2738 2782 4706 4723 4876 5061
Size: 1255 544 614 44 1924 17 153 185
Cuts at: 5061 5506 325
Size: 445 1498
Fragments arranged by size:
1924 1498 1255 614 544 445 185 153
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 4925 6637
Size: 274 80 33 51 1443 765 2183 1712
Cuts at: 6637 96
Size: 138
Fragments arranged by size:
2183 1712 1443 765 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3267 3299 3766 3798 6639 6671 3267
Size: 32 467 32 2841 32 3275
Fragments arranged by size:
3275 2841 467 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6679
XbaI T'CTAG_A
Cuts at: 4015 4015
Size: 6679
XhoI C'TCGA_G
Cuts at: 1943 2550 1943
Size: 607 6072
XmaI C'CCGG_G
Cuts at: 1870 4004 1870
Size: 2134 4545
XmnI GAAnn'nnTTC
Cuts at: 2307 4808 2307
Size: 2501 4178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4487 276
Size: 53 83 186 936 2953 2468
Fragments arranged by size:
2953 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PsrI RsrII SacII SanDI SexAI SrfI XcmI