Restriction map for
pFN21ASDA0230
(Circular) (Six-base) MAPSORT of: pFN21ASDA0230.seq Check: 7438 from: 1 to: 9166
pFN21ASDA0230 9166 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2828 6365 2828
Size: 3537 5629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3870 5828 6976 278
Size: 53 83 186 936 2334 1958 1148 2468
Fragments arranged by size:
2468 2334 1958 1148 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4110 5547 6509 1937
Size: 2173 1437 962 4594
Fragments arranged by size:
4594 2173 1437 962
Acc65I G'GTAC_C
Cuts at: 6487 6487
Size: 9166
AclI AA'CG_TT
Cuts at: 1563 3858 7293 7666 1563
Size: 2295 3435 373 3063
Fragments arranged by size:
3435 3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3768 5215 6124 6160 7229 8613 8881 1632
Size: 2136 1447 909 36 1069 1384 268 1917
Fragments arranged by size:
2136 1917 1447 1384 1069 909 268 36
AfeI AGC'GCT
Cuts at: 2033 2033
Size: 9166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8969
AflIII A'CryG_T
Cuts at: 1203 4224 4569 4764 5571 5883 8065 9042 1203
Size: 3021 345 195 807 312 2182 977 1327
Fragments arranged by size:
3021 2182 1327 977 807 345 312 195
AgeI A'CCGG_T
Cuts at: 7997 7997
Size: 9166
AhdI GACnn_n'nnGTC
Cuts at: 4147 7895 4147
Size: 3748 5418
AleI CACnn'nnGTG
Cuts at: 1166 2159 3596 3626 4520 5870 1166
Size: 993 1437 30 894 1350 4462
Fragments arranged by size:
4462 1437 1350 993 894 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2177 2192 3185 6015 1184
Size: 993 15 993 2830 4335
Fragments arranged by size:
4335 2830 993 993 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5435 5467 5435
Size: 32 9134
AlwNI CAG_nnn'CTG
Cuts at: 4162 4495 5425 6909 8481 4162
Size: 333 930 1484 1572 4847
Fragments arranged by size:
4847 1572 1484 930 333
ApaI G_GGCC'C
Cuts at: 2045 4642 5711 2045
Size: 2597 1069 5500
Fragments arranged by size:
5500 2597 1069
ApaLI G'TGCA_C
Cuts at: 7223 8379 7223
Size: 1156 8010
ApoI r'AATT_y
Cuts at: 1493 2353 3731 6475 6701 6853 8962 1493
Size: 860 1378 2744 226 152 2109 1697
Fragments arranged by size:
2744 2109 1697 1378 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7720 160
Size: 872 6688 1606
Fragments arranged by size:
6688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9166
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2501 2797 3677 4356 4623 5868
Size: 73 102 456 296 880 679 267 1245
Cuts at: 5868 6491 1870
Size: 623 4545
Fragments arranged by size:
4545 1245 880 679 623 456 296 267
102 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2944 2977 2944
Size: 33 9133
BamHI G'GATC_C
Cuts at: 4018 5520 5838 6496 6863 4018
Size: 1502 318 658 367 6321
Fragments arranged by size:
6321 1502 658 367 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2203 3456 3638 4259
Size: 325 217 627 262 154 1253 182 621
Cuts at: 4259 4292 5689 6487 7942 618
Size: 33 1397 798 1455 1842
Fragments arranged by size:
1842 1455 1397 1253 798 627 621 325
262 217 182 154 33
BanII G_rGCy'C
Cuts at: 1391 2045 2778 3678 4642 4922 5439 5711 6485
Size: 654 733 900 964 280 517 272 774
Cuts at: 6485 1391
Size: 4072
Fragments arranged by size:
4072 964 900 774 733 654 517 280
272
BbeI G_GCGC'C
Cuts at: 2053 2207 3642 4296 2053
Size: 154 1435 654 6923
Fragments arranged by size:
6923 1435 654 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2349 2894 3008 5717 5979 6209 928
Size: 971 450 545 114 2709 262 230 3885
Fragments arranged by size:
3885 2709 971 545 450 262 230 114
BbvCI CC'TCA_GC
Cuts at: 2276 6133 2276
Size: 3857 5309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4164 4198 4636 4670 7357 7391 1903
Size: 34 2227 34 438 34 2687 34 3678
Fragments arranged by size:
3678 2687 2227 438 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2316 4020 7058 8268 2316
Size: 1704 3038 1210 3214
Fragments arranged by size:
3214 3038 1704 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9166
BfrBI ATG'CAT
Cuts at: 2173 6158 6320 2173
Size: 3985 162 5019
Fragments arranged by size:
5019 3985 162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 4821 5225
Size: 107 122 71 631 605 433 2716 404
Cuts at: 5225 5706 7777 136
Size: 481 2071 1525
Fragments arranged by size:
2716 2071 1525 631 605 481 433 404
122 107 71
BglII A'GATC_T
Cuts at: 2224 9161 2224
Size: 6937 2229
BlpI GC'TnA_GC
Cuts at: 2879 6583 2879
Size: 3704 5462
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2196 4392 4642 5415 5711 7227 8383
Size: 255 151 2196 250 773 296 1516 1156
Cuts at: 8383 1790
Size: 2573
Fragments arranged by size:
2573 2196 1516 1156 773 296 255 250
151
BmgBI CAC'GTC
Cuts at: 1318 5884 1318
Size: 4566 4600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3590 5056 6087
Size: 416 53 83 399 24 2173 1466 1031
Cuts at: 6087 6897 7855 442
Size: 810 958 1753
Fragments arranged by size:
2173 1753 1466 1031 958 810 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2204 3021 3125 4648 7826 1239
Size: 781 184 817 104 1523 3178 2579
Fragments arranged by size:
3178 2579 1523 817 781 184 104
Bpu10I CC'TnA_GC
Cuts at: 2276 4529 6133 2276
Size: 2253 1604 5309
Fragments arranged by size:
5309 2253 1604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5015 6056 7291 8156 8454 5015
Size: 1041 1235 865 298 5727
Fragments arranged by size:
5727 1235 1041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3039 4433 5066 6036 7829 882
Size: 617 1540 1394 633 970 1793 2219
Fragments arranged by size:
2219 1793 1540 1394 970 633 617
BsaAI yAC'GTr
Cuts at: 493 1204 6463 6957 493
Size: 711 5259 494 2702
Fragments arranged by size:
5259 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 9160 9160
Size: 9166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 2204 3335 3639
Size: 53 83 186 936 517 154 1131 304
Cuts at: 3639 3867 4293 5825 6973 7355 7990 275
Size: 228 426 1532 1148 382 635 1451
Fragments arranged by size:
1532 1451 1148 1131 936 635 517 426
382 304 228 186 154 83 53
BsaWI w'CCGG_w
Cuts at: 2526 4005 4245 5340 7599 7997 8271 8418 8829
Size: 1479 240 1095 2259 398 274 147 411
Cuts at: 8829 2526
Size: 2863
Fragments arranged by size:
2863 2259 1479 1095 411 398 274 240
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4496 4526 5435 5465 1178
Size: 30 165 30 3093 30 909 30 4879
Fragments arranged by size:
4879 3093 909 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 9166
BseYI C'CCAG_C
Cuts at: 3614 4966 5161 5704 5802 8369 8865 3614
Size: 1352 195 543 98 2567 496 3915
Fragments arranged by size:
3915 2567 1352 543 496 195 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 3130 3599 3668 3679 4594 4865
Size: 573 693 701 469 69 11 915 271
Cuts at: 4865 5894 1163
Size: 1029 4435
Fragments arranged by size:
4435 1029 915 701 693 573 469 271
69 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6616 7377 8405 1540
Size: 275 186 4615 761 1028 2301
Fragments arranged by size:
4615 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3260 3849 4778 5007 5439 7227 7312 8383 3260
Size: 589 929 229 432 1788 85 1071 4043
Fragments arranged by size:
4043 1788 1071 929 589 432 229 85
BsmI GAATG_Cn'
Cuts at: 2264 3530 3609 6341 6677 6770 2264
Size: 1266 79 2732 336 93 4660
Fragments arranged by size:
4660 2732 1266 336 93 79
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2196 2778 3260 3678 3849 4392
Size: 399 255 151 582 482 418 171 543
Cuts at: 4392 4642 4778 4922 5007 5415 5439 5711 6485
Size: 250 136 144 85 408 24 272 774
Cuts at: 6485 7227 7312 8383 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 582 543 482 418
408 399 272 255 250 171 151 144
136 85 85 24
BspEI T'CCGG_A
Cuts at: 5340 8829 5340
Size: 3489 5677
BspHI T'CATG_A
Cuts at: 4602 4701 7055 8785 4602
Size: 99 2354 1730 4983
Fragments arranged by size:
4983 2354 1730 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2451 2828 3779 4281 6340 6365 6521 844
Size: 1607 377 951 502 2059 25 156 3489
Fragments arranged by size:
3489 2059 1607 951 502 377 156 25
BsrBI CCG'CTC
Cuts at: 1394 1823 4635 5446 7053 1394
Size: 429 2812 811 1607 3507
Fragments arranged by size:
3507 2812 1607 811 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2291 4067 5013 5292 7661 7835 65
Size: 1183 1043 1776 946 279 2369 174 1396
Fragments arranged by size:
2369 1776 1396 1183 1043 946 279 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3450 4200 5268 5700 7810 7997 1700
Size: 110 1640 750 1068 432 2110 187 2869
Fragments arranged by size:
2869 2110 1640 1068 750 432 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 4090 4380 96
Size: 2277 1717 290 4882
Fragments arranged by size:
4882 2277 1717 290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9166
BssSI C'ACGA_G
Cuts at: 2069 5672 7226 8238 2069
Size: 3603 1554 1012 2997
Fragments arranged by size:
3603 2997 1554 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2179 4572 794
Size: 1385 2393 5388
Fragments arranged by size:
5388 2393 1385
BstBI TT'CG_AA
Cuts at: 2007 7970 2007
Size: 5963 3203
BstEII G'GTnAC_C
Cuts at: 1184 3013 3155 6352 1184
Size: 1829 142 3197 3998
Fragments arranged by size:
3998 3197 1829 142
BstXI CCAn_nnnn'nTGG
Cuts at: 4522 4522
Size: 9166
BstYI r'GATC_y
Cuts at: 1972 2224 2250 2385 3138 3883 4018 5097 5133
Size: 252 26 135 753 745 135 1079 36
Cuts at: 5133 5520 5610 5838 5931 6496 6863 7248 7265
Size: 387 90 228 93 565 367 385 17
Cuts at: 7265 8717 8803 8815 9161 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1079 753 745 565 387 385
367 346 252 228 135 135 93 90
86 36 26 17 12
Bsu36I CC'TnA_GG
Cuts at: 3020 3339 3020
Size: 319 8847
BtgI C'CryG_G
Cuts at: 513 1065 2022 2100 2199 3134 3182 3369 3444
Size: 552 957 78 99 935 48 187 75
Cuts at: 3444 4515 4718 4819 4890 5019 5173 5223 5457
Size: 1071 203 101 71 129 154 50 234
Cuts at: 5457 5515 6080 513
Size: 58 565 3599
Fragments arranged by size:
3599 1071 957 935 565 552 234 203
187 154 129 101 99 78 75 71
58 50 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3397 4156 487
Size: 683 56 2171 759 5497
Fragments arranged by size:
5497 2171 759 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 4487 4558 5258 6682 7476 7496 2605
Size: 1882 71 700 1424 794 20 4275
Fragments arranged by size:
4275 1882 1424 794 700 71 20
ClaI AT'CG_AT
Cuts at: 1554 5364 1554
Size: 3810 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2967 3002 529
Size: 35 2403 35 6693
Fragments arranged by size:
6693 2403 35 35
DraI TTT'AAA
Cuts at: 2358 6470 2358
Size: 4112 5054
DraIII CAC_nnn'GTG
Cuts at: 4896 4992 5470 6264 6306 4896
Size: 96 478 794 42 7756
Fragments arranged by size:
7756 794 478 96 42
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5543 6350 8173 809
Size: 507 4227 807 1823 1802
Fragments arranged by size:
4227 1823 1802 807 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2097 2679
Size: 54 1425 50 275 18 195 72 582
Cuts at: 2679 2988 4809 4938 5239 5487 5832 6613 7502
Size: 309 1821 129 301 248 345 781 889
Cuts at: 7502 9131 8
Size: 1629 43
Fragments arranged by size:
1821 1629 1425 889 781 582 345 309
301 275 248 195 129 72 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6613 1537
Size: 275 4801 4090
Fragments arranged by size:
4801 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2570 2733 3321 6434 7096 1353
Size: 1217 163 588 3113 662 3423
Fragments arranged by size:
3423 3113 1217 662 588 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2648 5912 6461 7753 8127 8273
Size: 1064 138 1059 3264 549 1292 374 146
Cuts at: 8273 387
Size: 1280
Fragments arranged by size:
3264 1292 1280 1064 1059 549 374 146
138
EcoICRI GAG'CTC
Cuts at: 5437 5437
Size: 9166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2204 3021 3125 3768 4648 5215
Size: 393 388 184 817 104 643 880 567
Cuts at: 5215 6124 6160 7229 7826 8613 8881 1239
Size: 909 36 1069 597 787 268 1524
Fragments arranged by size:
1524 1069 909 880 817 787 643 597
567 393 388 268 184 104 36
EcoNI CCTnn'n_nnAGG
Cuts at: 4532 5141 4532
Size: 609 8557
EcoO109I rG'GnC_Cy
Cuts at: 2041 2042 2757 2922 4638 5581 5683 5708 6414
Size: 1 715 165 1716 943 102 25 706
Cuts at: 6414 2041
Size: 4793
Fragments arranged by size:
4793 1716 943 715 706 165 102 25
1
EcoRI G'AATT_C
Cuts at: 6475 6853 6475
Size: 378 8788
FspI TGC'GCA
Cuts at: 7672 7672
Size: 9166
HaeII r_GCGC'y
Cuts at: 1678 2035 2053 2069 2207 3117 3642 4296 4318
Size: 357 18 16 138 910 525 654 22
Cuts at: 4318 5672 5749 8313 1678
Size: 1354 77 2564 2531
Fragments arranged by size:
2564 2531 1354 910 654 525 357 138
77 22 18 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2542 2574 2780
Size: 32 135 32 803 32 135 32 206
Cuts at: 2780 2812 4133 4165 4496 4528 7807 7839 7881
Size: 32 1321 32 331 32 3279 32 42
Cuts at: 7881 7913 1373
Size: 32 2626
Fragments arranged by size:
3279 2626 1321 803 331 206 135 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 3538 3928 4111 5254 6510
Size: 988 281 1240 360 390 183 1143 1256
Cuts at: 6510 6756 669
Size: 246 3079
Fragments arranged by size:
3079 1256 1240 1143 988 390 360 281
246 183
HindIII A'AGCT_T
Cuts at: 748 5322 6369 748
Size: 4574 1047 3545
Fragments arranged by size:
4574 3545 1047
HpaI GTT'AAC
Cuts at: 6756 6756
Size: 9166
KasI G'GCGC_C
Cuts at: 2049 2203 3638 4292 2049
Size: 154 1435 654 6923
Fragments arranged by size:
6923 1435 654 154
KpnI G_GTAC'C
Cuts at: 6491 6491
Size: 9166
MfeI C'AATT_G
Cuts at: 2394 6765 2394
Size: 4371 4795
MluI A'CGCG_T
Cuts at: 9042 9042
Size: 9166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 4572 4785 8280 8464 1305
Size: 973 2294 213 3495 184 2007
Fragments arranged by size:
3495 2294 2007 973 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 4811 5241 5834 10
Size: 54 1425 343 195 2784 430 593 3342
Fragments arranged by size:
3342 2784 1425 593 430 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3596 3626 4469 4475 4520 5717
Size: 648 993 1437 30 843 6 45 1197
Cuts at: 5717 5870 7124 7483 7642 518
Size: 153 1254 359 159 2042
Fragments arranged by size:
2042 1437 1254 1197 993 843 648 359
159 153 45 30 6
MspA1I CmG'CkG
Cuts at: 1606 2055 2139 2678 3136 3184 3243 4644 4683
Size: 449 84 539 458 48 59 1401 39
Cuts at: 4683 4720 4821 4849 4864 4966 5225 5422 5503
Size: 37 101 28 15 102 259 197 81
Cuts at: 5503 5806 5922 6582 7259 8407 8652 1606
Size: 303 116 660 677 1148 245 2120
Fragments arranged by size:
2120 1401 1148 677 660 539 458 449
303 259 245 197 116 102 101 84
81 59 48 39 37 28 15
NaeI GCC'GGC
Cuts at: 5702 5702
Size: 9166
NarI GG'CG_CC
Cuts at: 2050 2204 3639 4293 2050
Size: 154 1435 654 6923
Fragments arranged by size:
6923 1435 654 154
NcoI C'CATG_G
Cuts at: 513 1065 2022 5457 513
Size: 552 957 3435 4222
Fragments arranged by size:
4222 3435 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9166
NgoMIV G'CCGG_C
Cuts at: 5700 5700
Size: 9166
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9166
NotI GC'GGCC_GC
Cuts at: 6613 6613
Size: 9166
NsiI A_TGCA'T
Cuts at: 2175 6160 6322 2175
Size: 3985 162 5019
Fragments arranged by size:
5019 3985 162
NspI r_CATG'y
Cuts at: 1132 1229 2173 4091 4228 4474 4651 4903 4984
Size: 97 944 1918 137 246 177 252 81
Cuts at: 4984 6324 6524 8069 1132
Size: 1340 200 1545 2229
Fragments arranged by size:
2229 1918 1545 1340 944 252 246 200
177 137 97 81
PasI CC'CwG_GG
Cuts at: 1348 3667 5783 5937 6410 1348
Size: 2319 2116 154 473 4104
Fragments arranged by size:
4104 2319 2116 473 154
PciI A'CATG_T
Cuts at: 4224 8065 4224
Size: 3841 5325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2974 3370 3693 4495 1108
Size: 1866 396 323 802 5779
Fragments arranged by size:
5779 1866 802 396 323
PfoI T'CCnGG_A
Cuts at: 3143 4494 4853 5613 3143
Size: 1351 359 760 6696
Fragments arranged by size:
6696 1351 760 359
PmeI GTTT'AAAC
Cuts at: 6470 6470
Size: 9166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5435 5467 6171 6203 7235 7267 8748
Size: 32 1201 32 704 32 1032 32 1481
Cuts at: 8748 8780 4202
Size: 32 4588
Fragments arranged by size:
4588 1481 1201 1032 704 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2922 5581 5683 2922
Size: 2659 102 6405
Fragments arranged by size:
6405 2659 102
PshAI GACnn'nnGTC
Cuts at: 1613 3789 3870 3969 1613
Size: 2176 81 99 6810
Fragments arranged by size:
6810 2176 99 81
PsiI TTA'TAA
Cuts at: 6736 6736
Size: 9166
PspOMI G'GGCC_C
Cuts at: 2041 4638 5707 2041
Size: 2597 1069 5500
Fragments arranged by size:
5500 2597 1069
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2795 2827 4697 4729 2795
Size: 32 1870 32 7232
Fragments arranged by size:
7232 1870 32 32
PstI C_TGCA'G
Cuts at: 830 2825 3784 4278 4565 6518 830
Size: 1995 959 494 287 1953 3478
Fragments arranged by size:
3478 1995 1953 959 494 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9166
PvuII CAG'CTG
Cuts at: 4966 5422 5806 5922 4966
Size: 456 384 116 8210
Fragments arranged by size:
8210 456 384 116
RsrII CG'GwC_CG
Cuts at: 4851 4851
Size: 9166
SacI G_AGCT'C
Cuts at: 5439 5439
Size: 9166
SacII CC_GC'GG
Cuts at: 3137 3185 4721 4822 5226 3137
Size: 48 1536 101 404 7077
Fragments arranged by size:
7077 1536 404 101 48
SalI G'TCGA_C
Cuts at: 1936 4109 6508 1936
Size: 2173 2399 4594
Fragments arranged by size:
4594 2399 2173
SanDI GG'GwC_CC
Cuts at: 2922 2922
Size: 9166
SapI GCTCTTCn'nnn_
Cuts at: 2733 3321 2733
Size: 588 8578
SbfI CC_TGCA'GG
Cuts at: 6518 6518
Size: 9166
ScaI AGT'ACT
Cuts at: 7414 9126 7414
Size: 1712 7454
SexAI A'CCwGG_T
Cuts at: 3459 4143 5685 3459
Size: 684 1542 6940
Fragments arranged by size:
6940 1542 684
SfcI C'TryA_G
Cuts at: 826 1045 2821 3780 4274 4561 5539 5977 6514
Size: 219 1776 959 494 287 978 438 537
Cuts at: 6514 7649 8330 8521 826
Size: 1135 681 191 1471
Fragments arranged by size:
1776 1471 1135 978 959 681 537 494
438 287 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2105 2105
Size: 9166
SfoI GGC'GCC
Cuts at: 2051 2205 3640 4294 2051
Size: 154 1435 654 6923
Fragments arranged by size:
6923 1435 654 154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9166
SgrAI Cr'CCGG_yG
Cuts at: 1700 4200 1700
Size: 2500 6666
SmaI CCC'GGG
Cuts at: 1872 2047 4625 5870 6493 1872
Size: 175 2578 1245 623 4545
Fragments arranged by size:
4545 2578 1245 623 175
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 5030 6035 7270 8171 8433
Size: 197 926 558 2529 1005 1235 901 262
Cuts at: 8433 820
Size: 1553
Fragments arranged by size:
2529 1553 1235 1005 926 901 558 262
197
SnaBI TAC'GTA
Cuts at: 493 6463 6957 493
Size: 5970 494 2702
Fragments arranged by size:
5970 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9166
SphI G_CATG'C
Cuts at: 1132 2173 4903 4984 6324 6524 1132
Size: 1041 2730 81 1340 200 3774
Fragments arranged by size:
3774 2730 1340 1041 200 81
SspI AAT'ATT
Cuts at: 5 52 7090 5
Size: 47 7038 2081
Fragments arranged by size:
7038 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9166
StyI C'CwwG_G
Cuts at: 513 1065 2022 3390 3423 5457 6605 513
Size: 552 957 1368 33 2034 1148 3074
Fragments arranged by size:
3074 2034 1368 1148 957 552 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4481 5078 7193 7210 7363 7548 7993
Size: 1255 2901 597 2115 17 153 185 445
Cuts at: 7993 325
Size: 1498
Fragments arranged by size:
2901 2115 1498 1255 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 2811 4090
Size: 274 80 33 51 1443 396 438 1279
Cuts at: 4090 4380 7412 9124 96
Size: 290 3032 1712 138
Fragments arranged by size:
3032 1712 1443 1279 438 396 290 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2314 2346 2703 2735 3139 3171 3365 3397 5392
Size: 32 357 32 404 32 194 32 1995
Cuts at: 5392 5424 5753 5785 9126 9158 2314
Size: 32 329 32 3341 32 2322
Fragments arranged by size:
3341 2322 1995 404 357 329 194 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3281 3347 1316
Size: 1965 66 7135
Fragments arranged by size:
7135 1965 66
XbaI T'CTAG_A
Cuts at: 2428 6502 2428
Size: 4074 5092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3690 4408 4963 5083 6265 3690
Size: 718 555 120 1182 6591
Fragments arranged by size:
6591 1182 718 555 120
XhoI C'TCGA_G
Cuts at: 1943 2501 1943
Size: 558 8608
XmaI C'CCGG_G
Cuts at: 1870 2045 4623 5868 6491 1870
Size: 175 2578 1245 623 4545
Fragments arranged by size:
4545 2578 1245 623 175
XmnI GAAnn'nnTTC
Cuts at: 4343 7295 4343
Size: 2952 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3868 5826 6974 276
Size: 53 83 186 936 2334 1958 1148 2468
Fragments arranged by size:
2468 2334 1958 1148 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BplI BsiWI BsmBI BstZ17I EcoRV FalI
FseI FspAI NruI PacI PmlI PspXI SrfI SwaI