Restriction map for
pFN21ASDA0223
(Circular) (Six-base) MAPSORT of: pFN21ASDA0223.seq Check: 7833 from: 1 to: 8137
pFN21ASDA0223 8137 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3434 3841 3434
Size: 407 7730
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2358 3962 5947 278
Size: 53 83 186 936 822 1604 1985 2468
Fragments arranged by size:
2468 1985 1604 936 822 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4185 5049 5480 1937
Size: 2248 864 431 4594
Fragments arranged by size:
4594 2248 864 431
Acc65I G'GTAC_C
Cuts at: 3971 5458 3971
Size: 1487 6650
AclI AA'CG_TT
Cuts at: 1563 6264 6637 1563
Size: 4701 373 3063
Fragments arranged by size:
4701 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2647 4008 4294 4625 6200 7584 7852 1632
Size: 1015 1361 286 331 1575 1384 268 1917
Fragments arranged by size:
1917 1575 1384 1361 1015 331 286 268
AfeI AGC'GCT
Cuts at: 2852 3257 2852
Size: 405 7732
AflII C'TTAA_G
Cuts at: 820 1017 2420 820
Size: 197 1403 6537
Fragments arranged by size:
6537 1403 197
AflIII A'CryG_T
Cuts at: 1203 4432 7036 8013 1203
Size: 3229 2604 977 1327
Fragments arranged by size:
3229 2604 1327 977
AgeI A'CCGG_T
Cuts at: 6968 6968
Size: 8137
AhdI GACnn_n'nnGTC
Cuts at: 3002 6866 3002
Size: 3864 4273
AleI CACnn'nnGTG
Cuts at: 1166 3428 1166
Size: 2262 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 3852 3884 3053
Size: 32 767 32 7306
Fragments arranged by size:
7306 767 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3064 3130 3460 3601 4747 5880 7452 3064
Size: 66 330 141 1146 1133 1572 3749
Fragments arranged by size:
3749 1572 1146 1133 330 141 66
ApaI G_GGCC'C
Cuts at: 2473 4830 2473
Size: 2357 5780
ApaLI G'TGCA_C
Cuts at: 3587 3686 3817 6194 7350 3587
Size: 99 131 2377 1156 4374
Fragments arranged by size:
4374 2377 1156 131 99
ApoI r'AATT_y
Cuts at: 1493 5423 5446 5672 5824 7933 1493
Size: 3930 23 226 152 2109 1697
Fragments arranged by size:
3930 2109 1697 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 6691 160
Size: 872 5659 1606
Fragments arranged by size:
5659 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8137
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2320 2737 4061 4544 4635 4802
Size: 73 164 213 417 1324 483 91 167
Cuts at: 4802 4967 5091 5268 5462 1870
Size: 165 124 177 194 4545
Fragments arranged by size:
4545 1324 483 417 213 194 177 167
165 164 124 91 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3459 3492 3459
Size: 33 8104
BamHI G'GATC_C
Cuts at: 5467 5834 5467
Size: 367 7770
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2564 3098 3186 3574
Size: 325 217 627 555 222 534 88 388
Cuts at: 3574 3971 5017 5458 6913 618
Size: 397 1046 441 1455 1842
Fragments arranged by size:
1842 1455 1046 627 555 534 441 397
388 325 222 217 88
BanII G_rGCy'C
Cuts at: 1391 2049 2303 2473 3018 3681 3705 3800 4581
Size: 658 254 170 545 663 24 95 781
Cuts at: 4581 4704 4830 5402 5456 1391
Size: 123 126 572 54 4072
Fragments arranged by size:
4072 781 663 658 572 545 254 170
126 123 95 54 24
BbeI G_GCGC'C
Cuts at: 3578 3578
Size: 8137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2805 3449 4933 928
Size: 971 906 644 1484 4132
Fragments arranged by size:
4132 1484 971 906 644
BbvCI CC'TCA_GC
Cuts at: 2403 5001 5177 2403
Size: 2598 176 5363
Fragments arranged by size:
5363 2598 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4804 4838 6328 6362 1903
Size: 34 2867 34 1490 34 3678
Fragments arranged by size:
3678 2867 1490 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3460 6029 7239 3460
Size: 2569 1210 4358
Fragments arranged by size:
4358 2569 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8137
BfrBI ATG'CAT
Cuts at: 2506 3562 2506
Size: 1056 7081
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2243 4628 5238
Size: 107 122 71 631 605 571 2385 610
Cuts at: 5238 6748 136
Size: 1510 1525
Fragments arranged by size:
2385 1525 1510 631 610 605 571 122
107 71
BglII A'GATC_T
Cuts at: 8132 8132
Size: 8137
BlpI GC'TnA_GC
Cuts at: 5554 5554
Size: 8137
Bme1580I G_kGCm'C
Cuts at: 1790 2473 2783 3101 3196 3591 3690 3745 3821
Size: 683 310 318 95 395 99 55 76
Cuts at: 3821 3830 4083 4269 4347 4830 5167 6198 7354
Size: 9 253 186 78 483 337 1031 1156
Cuts at: 7354 1790
Size: 2573
Fragments arranged by size:
2573 1156 1031 683 483 395 337 318
310 253 186 99 95 78 76 55
9
BmgBI CAC'GTC
Cuts at: 1318 2389 2839 3709 1318
Size: 1071 450 870 5746
Fragments arranged by size:
5746 1071 870 450
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 4077 5405
Size: 416 53 83 399 24 874 1786 1328
Cuts at: 5405 5868 6826 442
Size: 463 958 1753
Fragments arranged by size:
1786 1753 1328 958 874 463 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8137
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 5149 2386
Size: 2763 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2014 2183 2636 2663 2951 2969 3074 3086
Size: 775 169 453 27 288 18 105 12
Cuts at: 3086 3395 3482 4262 5048 5207 5276 6797 1239
Size: 309 87 780 786 159 69 1521 2579
Fragments arranged by size:
2579 1521 786 780 775 453 309 288
169 159 105 87 69 27 18 12
Bpu10I CC'TnA_GC
Cuts at: 2225 2403 5001 5177 2225
Size: 178 2598 176 5185
Fragments arranged by size:
5185 2598 178 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2432 2471 4505 6262 7127 7425 2192
Size: 240 39 2034 1757 865 298 2904
Fragments arranged by size:
2904 2034 1757 865 298 240 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4955 6800 882
Size: 617 3456 1845 2219
Fragments arranged by size:
3456 2219 1845 617
BsaAI yAC'GTr
Cuts at: 493 1204 3250 3652 5434 5928 493
Size: 711 2046 402 1782 494 2702
Fragments arranged by size:
2702 2046 1782 711 494 402
BsaBI GATnn'nnATC
Cuts at: 8131 8131
Size: 8137
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 2355 2808 3575
Size: 53 83 186 936 654 168 453 767
Cuts at: 3575 3959 4631 5944 6326 6961 275
Size: 384 672 1313 382 635 1451
Fragments arranged by size:
1451 1313 936 767 672 654 635 453
384 382 186 168 83 53
BsaWI w'CCGG_w
Cuts at: 2295 4137 6570 6968 7242 7389 7800 2295
Size: 1842 2433 398 274 147 411 2632
Fragments arranged by size:
2632 2433 1842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 2942 2972 4430
Size: 30 165 30 1014 30 495 30 1458
Cuts at: 4430 4460 1178
Size: 30 4855
Fragments arranged by size:
4855 1458 1014 495 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 3317 3320 3392 3395 4004 4864 4961
Size: 1657 474 3 72 3 609 860 97
Cuts at: 4961 4964 4973 5141 5261 1186
Size: 3 9 168 120 4062
Fragments arranged by size:
4062 1657 860 609 474 168 120 97
72 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2093 2174 2789 5304 7340 7836 2093
Size: 81 615 2515 2036 496 2394
Fragments arranged by size:
2515 2394 2036 615 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3107 3272 5101 1163
Size: 573 1371 165 1829 4199
Fragments arranged by size:
4199 1829 1371 573 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3782 5587 6348 7376 1540
Size: 275 186 1781 1805 761 1028 2301
Fragments arranged by size:
2301 1805 1781 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 3591 3690 3821 4581 4704 6198 6283 7354
Size: 1542 99 131 760 123 1494 85 1071
Cuts at: 7354 2049
Size: 2832
Fragments arranged by size:
2832 1542 1494 1071 760 131 123 99
85
BsmI GAATG_Cn'
Cuts at: 5648 5741 5648
Size: 93 8044
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 2639 3715 4238 2540
Size: 99 1076 523 6439
Fragments arranged by size:
6439 1076 523 99
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2303 2473 2783 3018 3101 3196
Size: 399 259 254 170 310 235 83 95
Cuts at: 3196 3591 3681 3690 3705 3745 3800 3821 3830
Size: 395 90 9 15 40 55 21 9
Cuts at: 3830 4083 4269 4347 4581 4704 4830 5167 5402
Size: 253 186 78 234 123 126 337 235
Cuts at: 5402 5456 6198 6283 7354 1391
Size: 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 395 337 310 259
254 253 235 235 234 186 170 126
123 95 90 85 83 78 55 54
40 21 15 9 9
BspEI T'CCGG_A
Cuts at: 4137 7800 4137
Size: 3663 4474
BspHI T'CATG_A
Cuts at: 2049 6026 7756 2049
Size: 3977 1730 2430
Fragments arranged by size:
3977 2430 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3234 3434 3841 4539 4676 4722 4755 5492
Size: 2390 200 407 698 137 46 33 737
Cuts at: 5492 844
Size: 3489
Fragments arranged by size:
3489 2390 737 698 407 200 137 46
33
BsrBI CCG'CTC
Cuts at: 1394 1823 3028 3886 3955 4381 4552 6024 1394
Size: 429 1205 858 69 426 171 1472 3507
Fragments arranged by size:
3507 1472 1205 858 429 426 171 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 6632 6806 65
Size: 1183 1323 4061 174 1396
Fragments arranged by size:
4061 1396 1323 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 2561 2810 4069 4131 4641 6781
Size: 110 535 216 249 1259 62 510 2140
Cuts at: 6781 6968 1700
Size: 187 2869
Fragments arranged by size:
2869 2140 1259 535 510 249 216 187
110 62
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8137
BssHII G'CGCG_C
Cuts at: 1925 3321 1925
Size: 1396 6741
BssSI C'ACGA_G
Cuts at: 2474 3152 3287 4100 4574 6197 7209 2474
Size: 678 135 813 474 1623 1012 3402
Fragments arranged by size:
3402 1623 1012 813 678 474 135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4747 794
Size: 3953 4184
BstBI TT'CG_AA
Cuts at: 2007 6941 2007
Size: 4934 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8137
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 3355 5167 2896
Size: 459 1812 5866
Fragments arranged by size:
5866 1812 459
BstYI r'GATC_y
Cuts at: 1972 3492 5467 5834 6219 6236 7688 7774 7786
Size: 1520 1975 367 385 17 1452 86 12
Cuts at: 7786 8132 1972
Size: 346 1977
Fragments arranged by size:
1977 1975 1520 1452 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2465 2541 2748 3984 4844 5377 513
Size: 552 1400 76 207 1236 860 533 3273
Fragments arranged by size:
3273 1400 1236 860 552 533 207 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7398
Fragments arranged by size:
7398 683 56
BtsI GCAGTG_nn'
Cuts at: 2727 2779 2876 4674 5248 5357 5653 6447 6467
Size: 52 97 1798 574 109 296 794 20
Cuts at: 6467 2727
Size: 4397
Fragments arranged by size:
4397 1798 794 574 296 109 97 52
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8102
DraI TTT'AAA
Cuts at: 5441 5441
Size: 8137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7144 809
Size: 507 5828 1802
Fragments arranged by size:
5828 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2898 3073 3291
Size: 54 1425 50 275 18 1068 175 218
Cuts at: 3291 3342 3411 3432 3534 3779 3881 4074 4251
Size: 51 69 21 102 245 102 193 177
Cuts at: 4251 4300 4590 4841 5584 6473 8102 8
Size: 49 290 251 743 889 1629 43
Fragments arranged by size:
1629 1425 1068 889 743 290 275 251
245 218 193 177 175 102 102 69
54 51 50 49 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3779 5584 1537
Size: 275 1967 1805 4090
Fragments arranged by size:
4090 1967 1805 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3743 4203 6067 1353
Size: 2390 460 1864 3423
Fragments arranged by size:
3423 2390 1864 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2399 3230 3314 3413 3703 4085
Size: 1064 138 810 831 84 99 290 382
Cuts at: 4085 4553 5087 5401 6724 7098 7244 387
Size: 468 534 314 1323 374 146 1280
Fragments arranged by size:
1323 1280 1064 831 810 534 468 382
374 314 290 146 138 99 84
EcoICRI GAG'CTC
Cuts at: 2047 4579 4702 2047
Size: 2532 123 5482
Fragments arranged by size:
5482 2532 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2014 2183 2636 2647 2663 2951 2969
Size: 393 382 169 453 11 16 288 18
Cuts at: 2969 3074 3086 3395 3482 4008 4262 4294 4625
Size: 105 12 309 87 526 254 32 331
Cuts at: 4625 5048 5207 5276 6200 6797 7584 7852 1239
Size: 423 159 69 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 526 453 423 393
382 331 309 288 268 254 169 159
105 87 69 32 18 16 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2450 2486 3056 3230 3930 5126 2450
Size: 36 570 174 700 1196 5461
Fragments arranged by size:
5461 1196 700 570 174 36
EcoO109I rG'GnC_Cy
Cuts at: 2178 2202 2407 2638 2914 2968 3061 4711 5353
Size: 24 205 231 276 54 93 1650 642
Cuts at: 5353 2178
Size: 4962
Fragments arranged by size:
4962 1650 642 276 231 205 93 54
24
EcoRI G'AATT_C
Cuts at: 5423 5446 5824 5423
Size: 23 378 7736
Fragments arranged by size:
7736 378 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3298 3772 3298
Size: 474 7663
FspI TGC'GCA
Cuts at: 3230 4394 6643 3230
Size: 1164 2249 4724
Fragments arranged by size:
4724 2249 1164
HaeII r_GCGC'y
Cuts at: 1678 2854 3259 3485 3578 4332 4391 7284 1678
Size: 1176 405 226 93 754 59 2893 2531
Fragments arranged by size:
2893 2531 1176 754 405 226 93 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2988 3020 3203 3235 3944
Size: 32 135 32 1416 32 183 32 709
Cuts at: 3944 3976 4094 4126 4970 5002 5099 5129 5131
Size: 32 118 32 844 32 97 30 2
Cuts at: 5131 5161 6778 6810 6852 6884 1373
Size: 30 1617 32 42 32 2626
Fragments arranged by size:
2626 1617 1416 844 709 183 135 118
97 42 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5481 5727 669
Size: 988 281 3543 246 3079
Fragments arranged by size:
3543 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8137
HpaI GTT'AAC
Cuts at: 5727 5727
Size: 8137
KasI G'GCGC_C
Cuts at: 3574 3574
Size: 8137
KpnI G_GTAC'C
Cuts at: 3975 5462 3975
Size: 1487 6650
MfeI C'AATT_G
Cuts at: 5736 5736
Size: 8137
MluI A'CGCG_T
Cuts at: 4432 8013 4432
Size: 3581 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3246 5013 5127 5361 7251 7435 1305
Size: 1941 1767 114 234 1890 184 2007
Fragments arranged by size:
2007 1941 1890 1767 234 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2900 3293 3413 4253 4592
Size: 54 1425 343 1068 393 120 840 339
Cuts at: 4592 10
Size: 3555
Fragments arranged by size:
3555 1425 1068 840 393 343 339 120
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3428 6095 6454 6613 518
Size: 648 2262 2667 359 159 2042
Fragments arranged by size:
2667 2262 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2174 2467 2530 3580 3661 3667 4294 5056
Size: 568 293 63 1050 81 6 627 762
Cuts at: 5056 5238 5254 5553 6230 7378 7623 1606
Size: 182 16 299 677 1148 245 2120
Fragments arranged by size:
2120 1148 1050 762 677 627 568 299
293 245 182 81 63 16 6
NaeI GCC'GGC
Cuts at: 2347 2563 2812 4643 2347
Size: 216 249 1831 5841
Fragments arranged by size:
5841 1831 249 216
NarI GG'CG_CC
Cuts at: 3575 3575
Size: 8137
NcoI C'CATG_G
Cuts at: 513 1065 2748 513
Size: 552 1683 5902
Fragments arranged by size:
5902 1683 552
NdeI CA'TA_TG
Cuts at: 387 3564 387
Size: 3177 4960
NgoMIV G'CCGG_C
Cuts at: 2345 2561 2810 4641 2345
Size: 216 249 1831 5841
Fragments arranged by size:
5841 1831 249 216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8137
NotI GC'GGCC_GC
Cuts at: 5584 5584
Size: 8137
NsiI A_TGCA'T
Cuts at: 2508 3564 2508
Size: 1056 7081
NspI r_CATG'y
Cuts at: 1132 1229 3025 5495 7040 1132
Size: 97 1796 2470 1545 2229
Fragments arranged by size:
2470 2229 1796 1545 97
PasI CC'CwG_GG
Cuts at: 1348 5167 1348
Size: 3819 4318
PciI A'CATG_T
Cuts at: 7036 7036
Size: 8137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 2749 3082 3723 4010 1108
Size: 1272 369 333 641 287 5235
Fragments arranged by size:
5235 1272 641 369 333 287
PfoI T'CCnGG_A
Cuts at: 2030 2273 3495 5184 2030
Size: 243 1222 1689 4983
Fragments arranged by size:
4983 1689 1222 243
PmeI GTTT'AAAC
Cuts at: 5441 5441
Size: 8137
PmlI CAC'GTG
Cuts at: 3652 3652
Size: 8137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6206 6238 7719 7751 4718
Size: 32 1456 32 1481 32 5104
Fragments arranged by size:
5104 1481 1456 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2178 2202 2914 3061 4711 2178
Size: 24 712 147 1650 5604
Fragments arranged by size:
5604 1650 712 147 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8137
PsiI TTA'TAA
Cuts at: 5707 5707
Size: 8137
PspOMI G'GGCC_C
Cuts at: 2469 4826 2469
Size: 2357 5780
PspXI vC'TCGA_Gb
Cuts at: 2737 2737
Size: 8137
PstI C_TGCA'G
Cuts at: 830 2976 2996 3666 4293 4743 5061 5352 5408
Size: 2146 20 670 627 450 318 291 56
Cuts at: 5408 5489 830
Size: 81 3478
Fragments arranged by size:
3478 2146 670 627 450 318 291 81
56 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8137
PvuII CAG'CTG
Cuts at: 3661 4294 5254 3661
Size: 633 960 6544
Fragments arranged by size:
6544 960 633
SacI G_AGCT'C
Cuts at: 2049 4581 4704 2049
Size: 2532 123 5482
Fragments arranged by size:
5482 2532 123
SacII CC_GC'GG
Cuts at: 2468 2468
Size: 8137
SalI G'TCGA_C
Cuts at: 1936 5479 1936
Size: 3543 4594
SanDI GG'GwC_CC
Cuts at: 2202 2202
Size: 8137
SapI GCTCTTCn'nnn_
Cuts at: 3743 3743
Size: 8137
SbfI CC_TGCA'GG
Cuts at: 5408 5489 5408
Size: 81 8056
ScaI AGT'ACT
Cuts at: 6385 8097 6385
Size: 1712 6425
SexAI A'CCwGG_T
Cuts at: 3864 3864
Size: 8137
SfcI C'TryA_G
Cuts at: 826 1045 2846 2972 2992 3662 4289 4739 5057
Size: 219 1801 126 20 670 627 450 318
Cuts at: 5057 5348 5404 5485 6620 7301 7492 826
Size: 291 56 81 1135 681 191 1471
Fragments arranged by size:
1801 1471 1135 681 670 627 450 318
291 219 191 126 81 56 20
SfoI GGC'GCC
Cuts at: 3576 3576
Size: 8137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8137
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8137
SmaI CCC'GGG
Cuts at: 1872 2322 4637 4804 4969 5270 5464 1872
Size: 450 2315 167 165 301 194 4545
Fragments arranged by size:
4545 2315 450 301 194 167 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 2411 2420 2450 2737 4520
Size: 197 926 264 204 9 30 287 1783
Cuts at: 4520 6241 7142 7404 820
Size: 1721 901 262 1553
Fragments arranged by size:
1783 1721 1553 926 901 287 264 262
204 197 30 9
SnaBI TAC'GTA
Cuts at: 493 5434 5928 493
Size: 4941 494 2702
Fragments arranged by size:
4941 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8137
SphI G_CATG'C
Cuts at: 1132 5495 1132
Size: 4363 3774
SrfI GCCC'GGGC
Cuts at: 2322 2322
Size: 8137
SspI AAT'ATT
Cuts at: 5 52 6061 5
Size: 47 6009 2081
Fragments arranged by size:
6009 2081 47
StuI AGG'CCT
Cuts at: 1517 3182 4454 4493 5132 1517
Size: 1665 1272 39 639 4522
Fragments arranged by size:
4522 1665 1272 639 39
StyI C'CwwG_G
Cuts at: 513 1065 2577 2748 2964 3294 3471 3837 4113
Size: 552 1512 171 216 330 177 366 276
Cuts at: 4113 4398 4593 4623 5576 513
Size: 285 195 30 953 3074
Fragments arranged by size:
3074 1512 953 552 366 330 285 276
216 195 177 171 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2474 2681 6164 6181 6334 6519 6964
Size: 1255 894 207 3483 17 153 185 445
Cuts at: 6964 325
Size: 1498
Fragments arranged by size:
3483 1498 1255 894 445 207 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 6383 8095
Size: 274 80 33 51 1443 927 3479 1712
Cuts at: 8095 96
Size: 138
Fragments arranged by size:
3479 1712 1443 927 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4430 4462 8097 8129 4430
Size: 32 3635 32 4438
Fragments arranged by size:
4438 3635 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2357 1316
Size: 1041 7096
XbaI T'CTAG_A
Cuts at: 5473 5473
Size: 8137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3352 3991 5215 3043
Size: 309 639 1224 5965
Fragments arranged by size:
5965 1224 639 309
XhoI C'TCGA_G
Cuts at: 1943 2737 1943
Size: 794 7343
XmaI C'CCGG_G
Cuts at: 1870 2320 4635 4802 4967 5268 5462 1870
Size: 450 2315 167 165 301 194 4545
Fragments arranged by size:
4545 2315 450 301 194 167 165
XmnI GAAnn'nnTTC
Cuts at: 6266 6266
Size: 8137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2356 3960 5945 276
Size: 53 83 186 936 822 1604 1985 2468
Fragments arranged by size:
2468 1985 1604 936 822 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NruI PacI PsrI RsrII SfiI SwaI