Restriction map for
pFN21ASDA0221
(Circular) (Six-base) MAPSORT of: pFN21ASDA0221.seq Check: 3270 from: 1 to: 8116
pFN21ASDA0221 8116 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4725 4725
Size: 8116
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3560 5926 278
Size: 53 83 186 936 2024 2366 2468
Fragments arranged by size:
2468 2366 2024 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 3573 5459 1937
Size: 1368 268 1886 4594
Fragments arranged by size:
4594 1886 1368 268
Acc65I G'GTAC_C
Cuts at: 3837 5437 3837
Size: 1600 6516
AclI AA'CG_TT
Cuts at: 1563 3341 6243 6616 1563
Size: 1778 2902 373 3063
Fragments arranged by size:
3063 2902 1778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3002 4418 6179 7563 7831 1632
Size: 1370 1416 1761 1384 268 1917
Fragments arranged by size:
1917 1761 1416 1384 1370 268
AfeI AGC'GCT
Cuts at: 3440 4454 3440
Size: 1014 7102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7919
AflIII A'CryG_T
Cuts at: 1203 3902 7015 7992 1203
Size: 2699 3113 977 1327
Fragments arranged by size:
3113 2699 1327 977
AgeI A'CCGG_T
Cuts at: 6947 6947
Size: 8116
AhdI GACnn_n'nnGTC
Cuts at: 6845 6845
Size: 8116
AleI CACnn'nnGTG
Cuts at: 1166 3905 1166
Size: 2739 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2676 1184
Size: 1492 6624
AlwNI CAG_nnn'CTG
Cuts at: 2397 2716 3784 3793 5859 7431 2397
Size: 319 1068 9 2066 1572 3082
Fragments arranged by size:
3082 2066 1572 1068 319 9
ApaI G_GGCC'C
Cuts at: 2046 2262 2547 2946 3533 4058 2046
Size: 216 285 399 587 525 6104
Fragments arranged by size:
6104 587 525 399 285 216
ApaLI G'TGCA_C
Cuts at: 3255 6173 7329 3255
Size: 2918 1156 4042
Fragments arranged by size:
4042 2918 1156
ApoI r'AATT_y
Cuts at: 1493 5425 5651 5803 7912 1493
Size: 3932 226 152 2109 1697
Fragments arranged by size:
3932 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6670 160
Size: 872 5638 1606
Fragments arranged by size:
5638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 2206 3922 4010 4333 4828 4866
Size: 73 239 24 1716 88 323 495 38
Cuts at: 4866 5441 1870
Size: 575 4545
Fragments arranged by size:
4545 1716 575 495 323 239 88 73
38 24
BamHI G'GATC_C
Cuts at: 5446 5813 5446
Size: 367 7749
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 3837 3911 4337 4406
Size: 325 217 627 309 1741 74 426 69
Cuts at: 4406 5081 5210 5437 6892 618
Size: 675 129 227 1455 1842
Fragments arranged by size:
1842 1741 1455 675 627 426 325 309
227 217 129 74 69
BanII G_rGCy'C
Cuts at: 1391 2046 2114 2228 2262 2547 2946 3533 4058
Size: 655 68 114 34 285 399 587 525
Cuts at: 4058 4214 4837 5435 1391
Size: 156 623 598 4072
Fragments arranged by size:
4072 655 623 598 587 525 399 285
156 114 68 34
BbeI G_GCGC'C
Cuts at: 2100 3915 4410 5214 2100
Size: 1815 495 804 5002
Fragments arranged by size:
5002 1815 804 495
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2358 2587 3500 3554 928
Size: 971 459 229 913 54 5490
Fragments arranged by size:
5490 971 913 459 229 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6307 6341 1903
Size: 34 4370 34 3678
Fragments arranged by size:
4370 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6008 7218 6008
Size: 1210 6906
BclI T'GATC_A
Cuts at: 1548 4959 1548
Size: 3411 4705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 2211 3537
Size: 107 122 71 631 605 506 33 1326
Cuts at: 3537 4053 4871 5265 6727 136
Size: 516 818 394 1462 1525
Fragments arranged by size:
1525 1462 1326 818 631 605 516 506
394 122 107 71 33
BglII A'GATC_T
Cuts at: 8111 8111
Size: 8116
BlpI GC'TnA_GC
Cuts at: 5533 5533
Size: 8116
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2262 2547 2785 2946 3259 3533 4058
Size: 256 216 285 238 161 313 274 525
Cuts at: 4058 4340 6177 7333 1790
Size: 282 1837 1156 2573
Fragments arranged by size:
2573 1837 1156 525 313 285 282 274
256 238 216 161
BmgBI CAC'GTC
Cuts at: 1318 2465 3193 5332 1318
Size: 1147 728 2139 4102
Fragments arranged by size:
4102 2139 1147 728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 3816 4052
Size: 416 53 83 399 24 1284 1115 236
Cuts at: 4052 5398 5847 6805 442
Size: 1346 449 958 1753
Fragments arranged by size:
1753 1346 1284 1115 958 449 416 399
236 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 2837 2873 2882 2978 4202 6776 1239
Size: 1346 252 36 9 96 1224 2574 2579
Fragments arranged by size:
2579 2574 1346 1224 252 96 36 9
Bpu10I CC'TnA_GC
Cuts at: 3771 3771
Size: 8116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2982 6241 7106 7404 2663
Size: 319 3259 865 298 3375
Fragments arranged by size:
3375 3259 865 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3350 6779 882
Size: 617 1851 3429 2219
Fragments arranged by size:
3429 2219 1851 617
BsaAI yAC'GTr
Cuts at: 493 1204 4303 4981 5355 5413 5907 493
Size: 711 3099 678 374 58 494 2702
Fragments arranged by size:
3099 2702 711 678 494 374 58
BsaBI GATnn'nnATC
Cuts at: 4427 8110 4427
Size: 3683 4433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2159 2916 3557
Size: 53 83 186 936 564 62 757 641
Cuts at: 3557 3912 4407 4536 4627 4662 5113 5189 5211
Size: 355 495 129 91 35 451 76 22
Cuts at: 5211 5923 6305 6940 275
Size: 712 382 635 1451
Fragments arranged by size:
1451 936 757 712 641 635 564 495
451 382 355 186 129 91 83 76
62 53 35 22
BsaWI w'CCGG_w
Cuts at: 4161 6549 6947 7221 7368 7779 4161
Size: 2388 398 274 147 411 4498
Fragments arranged by size:
4498 2388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 3803 3833 4196
Size: 30 165 30 1407 30 963 30 363
Cuts at: 4196 4226 1178
Size: 30 5068
Fragments arranged by size:
5068 1407 963 363 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2591 2834 2897 4334 1186
Size: 907 498 243 63 1437 4968
Fragments arranged by size:
4968 1437 907 498 243 63
BseYI C'CCAG_C
Cuts at: 2045 4051 4126 5144 5274 7319 7815 2045
Size: 2006 75 1018 130 2045 496 2346
Fragments arranged by size:
2346 2045 2006 1018 496 130 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 2668 3722 3883 4158 4478 4487
Size: 573 780 152 1054 161 275 320 9
Cuts at: 4487 1163
Size: 4792
Fragments arranged by size:
4792 1054 780 573 320 275 161 152
9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5566 6327 7355 1540
Size: 275 186 3565 761 1028 2301
Fragments arranged by size:
3565 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3259 3619 4596 6177 6262 7333 3259
Size: 360 977 1581 85 1071 4042
Fragments arranged by size:
4042 1581 1071 977 360 85
BsiWI C'GTAC_G
Cuts at: 2037 2037
Size: 8116
BsmI GAATG_Cn'
Cuts at: 5627 5720 5627
Size: 93 8023
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2114 2228 2262 2547 2785 2946
Size: 399 256 68 114 34 285 238 161
Cuts at: 2946 3259 3533 3619 4058 4214 4340 4596 4837
Size: 313 274 86 439 156 126 256 241
Cuts at: 4837 5435 6177 6262 7333 1391
Size: 598 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 598 439 399 313 285
274 256 256 241 238 161 156 126
114 86 85 68 34
BspEI T'CCGG_A
Cuts at: 7779 7779
Size: 8116
BspHI T'CATG_A
Cuts at: 4842 6005 7735 4842
Size: 1163 1730 5223
Fragments arranged by size:
5223 1730 1163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 4141 4725 4779 5471 844
Size: 1398 1899 584 54 692 3489
Fragments arranged by size:
3489 1899 1398 692 584 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 4705 4762 5368 6003 1394
Size: 429 2125 757 57 606 635 3507
Fragments arranged by size:
3507 2125 757 635 606 429 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 6611 6785 65
Size: 1183 5363 174 1396
Fragments arranged by size:
5363 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3601 3914 4914 4967 6760 6947 1700
Size: 110 1791 313 1000 53 1793 187 2869
Fragments arranged by size:
2869 1793 1791 1000 313 187 110 53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8116
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8116
BssSI C'ACGA_G
Cuts at: 3398 6176 7188 3398
Size: 2778 1012 4326
Fragments arranged by size:
4326 2778 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3784 4474 4744 794
Size: 2990 690 270 4166
Fragments arranged by size:
4166 2990 690 270
BstBI TT'CG_AA
Cuts at: 2007 6920 2007
Size: 4913 3203
BstEII G'GTnAC_C
Cuts at: 1184 2524 3917 1184
Size: 1340 1393 5383
Fragments arranged by size:
5383 1393 1340
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 4604 2029
Size: 2575 5541
BstYI r'GATC_y
Cuts at: 1972 2754 3654 5446 5813 6198 6215 7667 7753
Size: 782 900 1792 367 385 17 1452 86
Cuts at: 7753 7765 8111 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1792 1452 900 782 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3348 3633 4002 4802 513
Size: 552 2283 285 369 800 3827
Fragments arranged by size:
3827 2283 800 552 369 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3234 3613 487
Size: 683 56 2008 379 4990
Fragments arranged by size:
4990 2008 683 379 56
BtsI GCAGTG_nn'
Cuts at: 2711 4042 4230 5632 6426 6446 2711
Size: 1331 188 1402 794 20 4381
Fragments arranged by size:
4381 1402 1331 794 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2446 2481 529
Size: 35 1882 35 6164
Fragments arranged by size:
6164 1882 35 35
DraI TTT'AAA
Cuts at: 4614 5317 5420 4614
Size: 703 103 7310
Fragments arranged by size:
7310 703 103
DraIII CAC_nnn'GTG
Cuts at: 2503 3403 2503
Size: 900 7216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3364 3556 5398 7123 809
Size: 507 2048 192 1842 1725 1802
Fragments arranged by size:
2048 1842 1802 1725 507 192
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 3187 3561
Size: 54 1425 50 275 18 786 571 374
Cuts at: 3561 3930 4080 5563 6452 8081 8
Size: 369 150 1483 889 1629 43
Fragments arranged by size:
1629 1483 1425 889 786 571 374 369
275 150 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5563 1537
Size: 275 3751 4090
Fragments arranged by size:
4090 3751 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 3035 4112 6046 1353
Size: 792 890 1077 1934 3423
Fragments arranged by size:
3423 1934 1077 890 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4133 6703 7077 7223 387
Size: 1064 138 2544 2570 374 146 1280
Fragments arranged by size:
2570 2544 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2837 2873 2882 2978 3002 4202
Size: 393 953 252 36 9 96 24 1200
Cuts at: 4202 4418 6179 6776 7563 7831 1239
Size: 216 1761 597 787 268 1524
Fragments arranged by size:
1761 1524 1200 953 787 597 393 268
252 216 96 36 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 3316 3524 5070 5219 2534
Size: 782 208 1546 149 5431
Fragments arranged by size:
5431 1546 782 208 149
EcoO109I rG'GnC_Cy
Cuts at: 2371 2544 2857 2943 3022 3160 3202 3454 3529
Size: 173 313 86 79 138 42 252 75
Cuts at: 3529 2371
Size: 6958
Fragments arranged by size:
6958 313 252 173 138 86 79 75
42
EcoRI G'AATT_C
Cuts at: 5425 5803 5425
Size: 378 7738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3179 5143 3179
Size: 1964 6152
FseI GG_CCGG'CC
Cuts at: 4918 4918
Size: 8116
FspI TGC'GCA
Cuts at: 6622 6622
Size: 8116
HaeII r_GCGC'y
Cuts at: 1678 2100 3442 3915 4117 4185 4410 4456 5214
Size: 422 1342 473 202 68 225 46 758
Cuts at: 5214 5339 7263 1678
Size: 125 1924 2531
Fragments arranged by size:
2531 1924 1342 758 473 422 225 202
125 68 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3344 3376 3803 3835 5174
Size: 32 135 32 1772 32 427 32 1339
Cuts at: 5174 5206 5303 5335 6757 6789 6831 6863 1373
Size: 32 97 32 1422 32 42 32 2626
Fragments arranged by size:
2626 1772 1422 1339 427 135 97 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 4816 5460 5706 669
Size: 988 281 1368 1510 644 246 3079
Fragments arranged by size:
3079 1510 1368 988 644 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8116
HpaI GTT'AAC
Cuts at: 5706 5706
Size: 8116
KasI G'GCGC_C
Cuts at: 2096 3911 4406 5210 2096
Size: 1815 495 804 5002
Fragments arranged by size:
5002 1815 804 495
KpnI G_GTAC'C
Cuts at: 3841 5441 3841
Size: 1600 6516
MfeI C'AATT_G
Cuts at: 5715 5715
Size: 8116
MluI A'CGCG_T
Cuts at: 7992 7992
Size: 8116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4218 7230 7414 1305
Size: 2913 3012 184 2007
Fragments arranged by size:
3012 2913 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3932 10
Size: 54 1425 343 2100 4194
Fragments arranged by size:
4194 2100 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 3632 3905 4670 4853 5234 6074
Size: 648 861 1605 273 765 183 381 840
Cuts at: 6074 6433 6592 518
Size: 359 159 2042
Fragments arranged by size:
2042 1605 861 840 765 648 381 359
273 183 159
MspA1I CmG'CkG
Cuts at: 1606 2716 2730 2776 3430 3793 4024 4051 4238
Size: 1110 14 46 654 363 231 27 187
Cuts at: 4238 4485 5532 6209 7357 7602 1606
Size: 247 1047 677 1148 245 2120
Fragments arranged by size:
2120 1148 1110 1047 677 654 363 247
245 231 187 46 27 14
NaeI GCC'GGC
Cuts at: 4916 4969 4916
Size: 53 8063
NarI GG'CG_CC
Cuts at: 2097 3912 4407 5211 2097
Size: 1815 495 804 5002
Fragments arranged by size:
5002 1815 804 495
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7564
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8116
NgoMIV G'CCGG_C
Cuts at: 4914 4967 4914
Size: 53 8063
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8116
NotI GC'GGCC_GC
Cuts at: 5563 5563
Size: 8116
NspI r_CATG'y
Cuts at: 1132 1229 2897 3118 3769 3906 4477 4930 5474
Size: 97 1668 221 651 137 571 453 544
Cuts at: 5474 7019 1132
Size: 1545 2229
Fragments arranged by size:
2229 1668 1545 651 571 544 453 221
137 97
PasI CC'CwG_GG
Cuts at: 1348 2548 4309 1348
Size: 1200 1761 5155
Fragments arranged by size:
5155 1761 1200
PciI A'CATG_T
Cuts at: 3902 7015 3902
Size: 3113 5003
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 3403 4516 5039 5185 1108
Size: 1212 1083 1113 523 146 4039
Fragments arranged by size:
4039 1212 1113 1083 523 146
PfoI T'CCnGG_A
Cuts at: 2067 2562 2721 5137 2067
Size: 495 159 2416 5046
Fragments arranged by size:
5046 2416 495 159
PmeI GTTT'AAAC
Cuts at: 5420 5420
Size: 8116
PmlI CAC'GTG
Cuts at: 4981 4981
Size: 8116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2140 6185 6217 7698 7730 2108
Size: 32 4045 32 1481 32 2494
Fragments arranged by size:
4045 2494 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2857 3022 3160 3202 2371
Size: 486 165 138 42 7285
Fragments arranged by size:
7285 486 165 138 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8116
PsiI TTA'TAA
Cuts at: 5686 5686
Size: 8116
PspOMI G'GGCC_C
Cuts at: 2042 2258 2543 2942 3529 4054 2042
Size: 216 285 399 587 525 6104
Fragments arranged by size:
6104 587 525 399 285 216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4463 4495 4463
Size: 32 8084
PstI C_TGCA'G
Cuts at: 830 2277 2393 3780 3789 4152 4237 5074 5468
Size: 1447 116 1387 9 363 85 837 394
Cuts at: 5468 830
Size: 3478
Fragments arranged by size:
3478 1447 1387 837 394 363 116 85
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8116
PvuII CAG'CTG
Cuts at: 2776 3430 3793 4024 4051 2776
Size: 654 363 231 27 6841
Fragments arranged by size:
6841 654 363 231 27
SalI G'TCGA_C
Cuts at: 1936 3304 5458 1936
Size: 1368 2154 4594
Fragments arranged by size:
4594 2154 1368
SapI GCTCTTCn'nnn_
Cuts at: 4112 4112
Size: 8116
SbfI CC_TGCA'GG
Cuts at: 4152 5468 4152
Size: 1316 6800
ScaI AGT'ACT
Cuts at: 6364 8076 6364
Size: 1712 6404
SexAI A'CCwGG_T
Cuts at: 4461 5013 4461
Size: 552 7564
SfcI C'TryA_G
Cuts at: 826 1045 2273 2389 3776 3785 4148 4233 5070
Size: 219 1228 116 1387 9 363 85 837
Cuts at: 5070 5464 6599 7280 7471 826
Size: 394 1135 681 191 1471
Fragments arranged by size:
1471 1387 1228 1135 837 681 394 363
219 191 116 85 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2178 2178
Size: 8116
SfoI GGC'GCC
Cuts at: 2098 3913 4408 5212 2098
Size: 1815 495 804 5002
Fragments arranged by size:
5002 1815 804 495
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
SgrAI Cr'CCGG_yG
Cuts at: 1700 3914 1700
Size: 2214 5902
SmaI CCC'GGG
Cuts at: 1872 2184 2208 4830 4868 5443 1872
Size: 312 24 2622 38 575 4545
Fragments arranged by size:
4545 2622 575 312 38 24
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 2997 6220 7121 7383 820
Size: 197 926 735 319 3223 901 262 1553
Fragments arranged by size:
3223 1553 926 901 735 319 262 197
SnaBI TAC'GTA
Cuts at: 493 5413 5907 493
Size: 4920 494 2702
Fragments arranged by size:
4920 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8116
SphI G_CATG'C
Cuts at: 1132 3769 5474 1132
Size: 2637 1705 3774
Fragments arranged by size:
3774 2637 1705
SrfI GCCC'GGGC
Cuts at: 2184 2208 2184
Size: 24 8092
SspI AAT'ATT
Cuts at: 5 52 6040 5
Size: 47 5988 2081
Fragments arranged by size:
5988 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8116
StyI C'CwwG_G
Cuts at: 513 1065 4083 4597 5302 5555 513
Size: 552 3018 514 705 253 3074
Fragments arranged by size:
3074 3018 705 552 514 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3242 3986 4373 5041 6143 6160 6313
Size: 1255 1662 744 387 668 1102 17 153
Cuts at: 6313 6498 6943 325
Size: 185 445 1498
Fragments arranged by size:
1662 1498 1255 1102 744 668 445 387
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4470 4929 6362
Size: 274 80 33 51 1443 2493 459 1433
Cuts at: 6362 8074 96
Size: 1712 138
Fragments arranged by size:
2493 1712 1443 1433 459 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2810 2842 3281 3313 8076 8108 2810
Size: 32 439 32 4763 32 2818
Fragments arranged by size:
4763 2818 439 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8116
XbaI T'CTAG_A
Cuts at: 5452 5452
Size: 8116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 4949 5182 2317
Size: 2632 233 5251
Fragments arranged by size:
5251 2632 233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8116
XmaI C'CCGG_G
Cuts at: 1870 2182 2206 4828 4866 5441 1870
Size: 312 24 2622 38 575 4545
Fragments arranged by size:
4545 2622 575 312 38 24
XmnI GAAnn'nnTTC
Cuts at: 2450 2473 2936 4559 6245 2450
Size: 23 463 1623 1686 4321
Fragments arranged by size:
4321 1686 1623 463 23
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3558 5924 276
Size: 53 83 186 936 2024 2366 2468
Fragments arranged by size:
2468 2366 2024 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FspAI NruI NsiI PacI
PspXI RsrII SacI SacII SanDI SwaI