Restriction map for
pFN21ASDA0218
(Circular) (Six-base) MAPSORT of: pFN21ASDA0218.seq Check: 4036 from: 1 to: 7012
pFN21ASDA0218 7012 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4822 278
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4355 1937
Size: 2418 4594
Acc65I G'GTAC_C
Cuts at: 4333 4333
Size: 7012
AclI AA'CG_TT
Cuts at: 1563 5139 5512 1563
Size: 3576 373 3063
Fragments arranged by size:
3576 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2508 2606 3069 4046 5075 6459 6727
Size: 708 168 98 463 977 1029 1384 268
Cuts at: 6727 1632
Size: 1917
Fragments arranged by size:
1917 1384 1029 977 708 463 268 168
98
AflII C'TTAA_G
Cuts at: 820 1017 2432 3769 820
Size: 197 1415 1337 4063
Fragments arranged by size:
4063 1415 1337 197
AflIII A'CryG_T
Cuts at: 1203 4041 5911 6888 1203
Size: 2838 1870 977 1327
Fragments arranged by size:
2838 1870 1327 977
AgeI A'CCGG_T
Cuts at: 5843 5843
Size: 7012
AhdI GACnn_n'nnGTC
Cuts at: 5741 5741
Size: 7012
AleI CACnn'nnGTG
Cuts at: 1166 4061 1166
Size: 2895 4117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7012
AlwNI CAG_nnn'CTG
Cuts at: 2753 4755 6327 2753
Size: 2002 1572 3438
Fragments arranged by size:
3438 2002 1572
ApaI G_GGCC'C
Cuts at: 2169 2294 2169
Size: 125 6887
ApaLI G'TGCA_C
Cuts at: 3826 5069 6225 3826
Size: 1243 1156 4613
Fragments arranged by size:
4613 1243 1156
ApoI r'AATT_y
Cuts at: 1493 2477 3352 3582 3608 4321 4547 4699 6808
Size: 984 875 230 26 713 226 152 2109
Cuts at: 6808 1493
Size: 1697
Fragments arranged by size:
2109 1697 984 875 713 230 226 152
26
AseI AT'TA_AT
Cuts at: 160 1032 5566 160
Size: 872 4534 1606
Fragments arranged by size:
4534 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
AvaI C'yCGr_G
Cuts at: 1870 1943 2340 2646 4092 4198 4337 1870
Size: 73 397 306 1446 106 139 4545
Fragments arranged by size:
4545 1446 397 306 139 106 73
BamHI G'GATC_C
Cuts at: 2363 4342 4709 2363
Size: 1979 367 4666
Fragments arranged by size:
4666 1979 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3774 4333 5788 618
Size: 325 217 627 1987 559 1455 1842
Fragments arranged by size:
1987 1842 1455 627 559 325 217
BanII G_rGCy'C
Cuts at: 1391 2109 2169 2240 2294 2570 2883 4020 4331
Size: 718 60 71 54 276 313 1137 311
Cuts at: 4331 1391
Size: 4072
Fragments arranged by size:
4072 1137 718 313 311 276 71 60
54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2346 928
Size: 971 447 5594
Fragments arranged by size:
5594 971 447
BbvCI CC'TCA_GC
Cuts at: 2493 2493
Size: 7012
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5203 5237 1903
Size: 34 3266 34 3678
Fragments arranged by size:
3678 3266 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4904 6114 4904
Size: 1210 5802
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7012
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2648 4088 5623
Size: 107 122 71 631 605 976 1440 1535
Cuts at: 5623 136
Size: 1525
Fragments arranged by size:
1535 1525 1440 976 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3036 3931 7007 3036
Size: 895 3076 3041
Fragments arranged by size:
3076 3041 895
BlpI GC'TnA_GC
Cuts at: 4429 4429
Size: 7012
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2294 3830 3963 5073 6229 1790
Size: 379 125 1536 133 1110 1156 2573
Fragments arranged by size:
2573 1536 1156 1110 379 133 125
BmgBI CAC'GTC
Cuts at: 1318 2067 1318
Size: 749 6263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2865 4743 5701
Size: 416 53 83 399 24 1448 1878 958
Cuts at: 5701 442
Size: 1753
Fragments arranged by size:
1878 1753 1448 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7012
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3055 3019
Size: 36 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2110 2181 3116 3298 3358 3503 4139
Size: 838 33 71 935 182 60 145 636
Cuts at: 4139 5672 1239
Size: 1533 2579
Fragments arranged by size:
2579 1533 935 838 636 182 145 71
60 33
Bpu10I CC'TnA_GC
Cuts at: 2493 2493
Size: 7012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2844 4123 5137 6002 6300 2844
Size: 1279 1014 865 298 3556
Fragments arranged by size:
3556 1279 1014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2553 5675 882
Size: 617 1054 3122 2219
Fragments arranged by size:
3122 2219 1054 617
BsaAI yAC'GTr
Cuts at: 493 1204 4309 4803 493
Size: 711 3105 494 2702
Fragments arranged by size:
3105 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3930 7006 3930
Size: 3076 3936
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 4819 5201 5836
Size: 53 83 186 936 494 2792 382 635
Cuts at: 5836 275
Size: 1451
Fragments arranged by size:
2792 1451 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5445 5843 6117 6264 6675 5445
Size: 398 274 147 411 5782
Fragments arranged by size:
5782 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 2552 2582 3578
Size: 30 165 30 846 30 273 30 996
Cuts at: 3578 3608 3659 3689 4129 4159 1178
Size: 30 51 30 440 30 4031
Fragments arranged by size:
4031 996 846 440 273 165 51 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2254 2257 2429 2813 2836 2891 3047
Size: 1065 3 3 172 384 23 55 156
Cuts at: 3047 3126 3437 3512 3683 4066 1186
Size: 79 311 75 171 383 4132
Fragments arranged by size:
4132 1065 384 383 311 172 171 156
79 75 55 23 3 3
BseYI C'CCAG_C
Cuts at: 2168 2675 6215 6711 2168
Size: 507 3540 496 2469
Fragments arranged by size:
3540 2469 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6439
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4462 5223 6251 1540
Size: 275 186 2461 761 1028 2301
Fragments arranged by size:
2461 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3000 3830 5073 5158 6229 3000
Size: 830 1243 85 1071 3783
Fragments arranged by size:
3783 1243 1071 830 85
BsmI GAATG_Cn'
Cuts at: 4523 4616 4523
Size: 93 6919
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2169 2240 2294 2570 2883 3000
Size: 399 319 60 71 54 276 313 117
Cuts at: 3000 3830 3963 4020 4331 5073 5158 6229 1391
Size: 830 133 57 311 742 85 1071 2174
Fragments arranged by size:
2174 1071 830 742 399 319 313 311
276 133 117 85 71 60 57 54
BspEI T'CCGG_A
Cuts at: 6675 6675
Size: 7012
BspHI T'CATG_A
Cuts at: 3945 4901 6631 3945
Size: 956 1730 4326
Fragments arranged by size:
4326 1730 956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4367 844
Size: 3523 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 4899 1394
Size: 429 212 2864 3507
Fragments arranged by size:
3507 2864 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 3985 5507 5681 65
Size: 1183 2737 1522 174 1396
Fragments arranged by size:
2737 1522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 3995 5656 5843 1700
Size: 110 320 1865 1661 187 2869
Fragments arranged by size:
2869 1865 1661 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 3144 96
Size: 3048 3964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7012
BssSI C'ACGA_G
Cuts at: 5072 6084 5072
Size: 1012 6000
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7012
BstBI TT'CG_AA
Cuts at: 2007 5816 2007
Size: 3809 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7012
BstXI CCAn_nnnn'nTGG
Cuts at: 3322 3322
Size: 7012
BstYI r'GATC_y
Cuts at: 1972 2363 3036 3685 3931 3975 4110 4342 4709
Size: 391 673 649 246 44 135 232 367
Cuts at: 4709 5094 5111 6563 6649 6661 7007 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 673 649 391 385 367 346
246 232 135 86 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 3603 3780 3603
Size: 177 6835
BtgI C'CryG_G
Cuts at: 513 1065 2022 2680 513
Size: 552 957 658 4845
Fragments arranged by size:
4845 957 658 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6273
Fragments arranged by size:
6273 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 3910 4068 4528 5322 5342 2751
Size: 1159 158 460 794 20 4421
Fragments arranged by size:
4421 1159 794 460 158 20
ClaI AT'CG_AT
Cuts at: 1554 2615 1554
Size: 1061 5951
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2268 2303 529
Size: 35 1704 35 5238
Fragments arranged by size:
5238 1704 35 35
DraI TTT'AAA
Cuts at: 4316 4316
Size: 7012
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6019 809
Size: 507 4703 1802
Fragments arranged by size:
4703 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 4459 5348
Size: 54 1425 50 275 18 302 2327 889
Cuts at: 5348 6977 8
Size: 1629 43
Fragments arranged by size:
2327 1629 1425 889 302 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4459 1537
Size: 275 2647 4090
Fragments arranged by size:
4090 2647 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 3010 3245 4942 1353
Size: 1184 473 235 1697 3423
Fragments arranged by size:
3423 1697 1184 473 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5599 5973 6119 387
Size: 1064 138 4010 374 146 1280
Fragments arranged by size:
4010 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2110 2181 2340 2508 2606 3069
Size: 393 445 33 71 159 168 98 463
Cuts at: 3069 3116 3298 3358 3503 4046 4139 5075 5672
Size: 47 182 60 145 543 93 936 597
Cuts at: 5672 6459 6727 1239
Size: 787 268 1524
Fragments arranged by size:
1524 936 787 597 543 463 445 393
268 182 168 159 145 98 93 71
60 47 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3444 3497 3665 3444
Size: 53 168 6791
Fragments arranged by size:
6791 168 53
EcoO109I rG'GnC_Cy
Cuts at: 2165 2291 3561 3699 2165
Size: 126 1270 138 5478
Fragments arranged by size:
5478 1270 138 126
EcoRI G'AATT_C
Cuts at: 3352 4321 4699 3352
Size: 969 378 5665
Fragments arranged by size:
5665 969 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3916 3916
Size: 7012
FspI TGC'GCA
Cuts at: 5518 5518
Size: 7012
HaeII r_GCGC'y
Cuts at: 1678 2185 6159 1678
Size: 507 3974 2531
Fragments arranged by size:
3974 2531 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 2900 2932 2933
Size: 32 135 32 980 32 316 32 1
Cuts at: 2933 2965 5653 5685 5727 5759 1373
Size: 32 2688 32 42 32 2626
Fragments arranged by size:
2688 2626 980 316 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4356 4602 669
Size: 988 281 2418 246 3079
Fragments arranged by size:
3079 2418 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7012
HpaI GTT'AAC
Cuts at: 4602 4602
Size: 7012
KpnI G_GTAC'C
Cuts at: 4337 4337
Size: 7012
MfeI C'AATT_G
Cuts at: 2511 4611 2511
Size: 2100 4912
MluI A'CGCG_T
Cuts at: 6888 6888
Size: 7012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 6126 6310 1305
Size: 744 4077 184 2007
Fragments arranged by size:
4077 2007 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5190
Fragments arranged by size:
5190 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4061 4970 5329 5488 518
Size: 648 2880 15 909 359 159 2042
Fragments arranged by size:
2880 2042 909 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2094 2486 3871 3877 4428 5105 6253 6498
Size: 488 392 1385 6 551 677 1148 245
Cuts at: 6498 1606
Size: 2120
Fragments arranged by size:
2120 1385 1148 677 551 488 392 245
6
NaeI GCC'GGC
Cuts at: 2132 2132
Size: 7012
NcoI C'CATG_G
Cuts at: 513 1065 2022 2680 513
Size: 552 957 658 4845
Fragments arranged by size:
4845 957 658 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7012
NgoMIV G'CCGG_C
Cuts at: 2130 2130
Size: 7012
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7012
NotI GC'GGCC_GC
Cuts at: 4459 4459
Size: 7012
NspI r_CATG'y
Cuts at: 1132 1229 3535 4045 4370 5915 1132
Size: 97 2306 510 325 1545 2229
Fragments arranged by size:
2306 2229 1545 510 325 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7012
PciI A'CATG_T
Cuts at: 4041 5911 4041
Size: 1870 5142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3177 1108
Size: 2069 4943
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 7012
PmeI GTTT'AAAC
Cuts at: 4316 4316
Size: 7012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5081 5113 6594 6626 5081
Size: 32 1481 32 5467
Fragments arranged by size:
5467 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3561 3561
Size: 7012
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7012
PsiI TTA'TAA
Cuts at: 4582 4582
Size: 7012
PspOMI G'GGCC_C
Cuts at: 2165 2290 2165
Size: 125 6887
PstI C_TGCA'G
Cuts at: 830 2429 2725 3876 4364 830
Size: 1599 296 1151 488 3478
Fragments arranged by size:
3478 1599 1151 488 296
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7012
PvuII CAG'CTG
Cuts at: 2094 2486 3871 3877 2094
Size: 392 1385 6 5229
Fragments arranged by size:
5229 1385 392 6
RsrII CG'GwC_CG
Cuts at: 4219 4219
Size: 7012
SalI G'TCGA_C
Cuts at: 1936 4354 1936
Size: 2418 4594
SapI GCTCTTCn'nnn_
Cuts at: 3245 3245
Size: 7012
SbfI CC_TGCA'GG
Cuts at: 4364 4364
Size: 7012
ScaI AGT'ACT
Cuts at: 4031 5260 6972 4031
Size: 1229 1712 4071
Fragments arranged by size:
4071 1712 1229
SfcI C'TryA_G
Cuts at: 826 1045 2425 2721 3291 3733 3872 4297 4360
Size: 219 1380 296 570 442 139 425 63
Cuts at: 4360 5495 6176 6367 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1380 1135 681 570 442 425 296
219 191 139 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7012
SmaI CCC'GGG
Cuts at: 1872 4094 4200 4339 1872
Size: 2222 106 139 4545
Fragments arranged by size:
4545 2222 139 106
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 2859 3769 4102 5116 6017
Size: 197 926 489 427 910 333 1014 901
Cuts at: 6017 6279 820
Size: 262 1553
Fragments arranged by size:
1553 1014 926 910 901 489 427 333
262 197
SnaBI TAC'GTA
Cuts at: 493 4309 4803 493
Size: 3816 494 2702
Fragments arranged by size:
3816 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7012
SphI G_CATG'C
Cuts at: 1132 4370 1132
Size: 3238 3774
SspI AAT'ATT
Cuts at: 5 52 4936 5
Size: 47 4884 2081
Fragments arranged by size:
4884 2081 47
StuI AGG'CCT
Cuts at: 1517 3329 1517
Size: 1812 5200
StyI C'CwwG_G
Cuts at: 513 1065 2022 2680 2884 3155 3556 3900 4263
Size: 552 957 658 204 271 401 344 363
Cuts at: 4263 4451 513
Size: 188 3074
Fragments arranged by size:
3074 957 658 552 401 363 344 271
204 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2907 5039 5056 5209 5394 5839 325
Size: 1255 1327 2132 17 153 185 445 1498
Fragments arranged by size:
2132 1498 1327 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3144 4029 5258
Size: 274 80 33 51 1443 1167 885 1229
Cuts at: 5258 6970 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1229 1167 885 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6972 7004 6972
Size: 32 6980
Tth111I GACn'n_nGTC
Cuts at: 1316 3122 1316
Size: 1806 5206
XbaI T'CTAG_A
Cuts at: 2410 4348 2410
Size: 1938 5074
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 4045 2389
Size: 1656 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7012
XmaI C'CCGG_G
Cuts at: 1870 4092 4198 4337 1870
Size: 2222 106 139 4545
Fragments arranged by size:
4545 2222 139 106
XmnI GAAnn'nnTTC
Cuts at: 5141 5141
Size: 7012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4820 276
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI