Restriction map for
pFN21ASDA0216
(Circular) (Six-base) MAPSORT of: pFN21ASDA0216.seq Check: 7563 from: 1 to: 10891
pFN21ASDA0216 10891 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6113 7182 6113
Size: 1069 9822
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8701 278
Size: 53 83 186 936 7165 2468
Fragments arranged by size:
7165 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 4386 8234 1937
Size: 694 1755 3848 4594
Fragments arranged by size:
4594 3848 1755 694
Acc65I G'GTAC_C
Cuts at: 8212 8212
Size: 10891
AclI AA'CG_TT
Cuts at: 1563 9018 9391 1563
Size: 7455 373 3063
Fragments arranged by size:
7455 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4089 6050 6161 6536 7016 7067 7217 8954
Size: 2457 1961 111 375 480 51 150 1737
Cuts at: 8954 10338 10606 1632
Size: 1384 268 1917
Fragments arranged by size:
2457 1961 1917 1737 1384 480 375 268
150 111 51
AfeI AGC'GCT
Cuts at: 2435 3305 4196 7209 2435
Size: 870 891 3013 6117
Fragments arranged by size:
6117 3013 891 870
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10694
AflIII A'CryG_T
Cuts at: 1203 3327 3402 9790 10767 1203
Size: 2124 75 6388 977 1327
Fragments arranged by size:
6388 2124 1327 977 75
AgeI A'CCGG_T
Cuts at: 9722 9722
Size: 10891
AhdI GACnn_n'nnGTC
Cuts at: 6967 9620 6967
Size: 2653 8238
AleI CACnn'nnGTG
Cuts at: 1166 4179 4270 4505 1166
Size: 3013 91 235 7552
Fragments arranged by size:
7552 3013 235 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2756 3348 4288 4523 7329 1184
Size: 1572 592 940 235 2806 4746
Fragments arranged by size:
4746 2806 1572 940 592 235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 7094 7126 2159
Size: 32 4903 32 5924
Fragments arranged by size:
5924 4903 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2979 3553 3565 3625 4618 5511 5668 5818 5956
Size: 574 12 60 993 893 157 150 138
Cuts at: 5956 6325 6724 7399 7774 8015 8055 8634 10206
Size: 369 399 675 375 241 40 579 1572
Cuts at: 10206 2979
Size: 3664
Fragments arranged by size:
3664 1572 993 893 675 579 574 399
375 369 241 157 150 138 60 40
12
ApaI G_GGCC'C
Cuts at: 2756 3373 4108 4220 2756
Size: 617 735 112 9427
Fragments arranged by size:
9427 735 617 112
ApaLI G'TGCA_C
Cuts at: 4263 5153 8948 10104 4263
Size: 890 3795 1156 5050
Fragments arranged by size:
5050 3795 1156 890
ApoI r'AATT_y
Cuts at: 1493 8200 8426 8578 10687 1493
Size: 6707 226 152 2109 1697
Fragments arranged by size:
6707 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2859 3669 9445 160
Size: 872 1827 810 5776 1606
Fragments arranged by size:
5776 1827 1606 872 810
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10891
AvaI C'yCGr_G
Cuts at: 1870 1943 2302 2595 2756 4220 4393 5248 5831
Size: 73 359 293 161 1464 173 855 583
Cuts at: 5831 6254 7332 8216 1870
Size: 423 1078 884 4545
Fragments arranged by size:
4545 1464 1078 884 855 583 423 359
293 173 161 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3248 3281 3248
Size: 33 10858
BamHI G'GATC_C
Cuts at: 8221 8588 8221
Size: 367 10524
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2586 2653 2807 2833 3433
Size: 325 217 627 799 67 154 26 600
Cuts at: 3433 3783 3842 4184 4687 4823 5546 8212 9667
Size: 350 59 342 503 136 723 2666 1455
Cuts at: 9667 618
Size: 1842
Fragments arranged by size:
2666 1842 1455 799 723 627 600 503
350 342 325 217 154 136 67 59
26
BanII G_rGCy'C
Cuts at: 1391 2602 2756 2961 2970 3135 3373 3861 4108
Size: 1211 154 205 9 165 238 488 247
Cuts at: 4108 4220 4311 4345 5196 5274 6295 6423 6591
Size: 112 91 34 851 78 1021 128 168
Cuts at: 6591 8210 1391
Size: 1619 4072
Fragments arranged by size:
4072 1619 1211 1021 851 488 247 238
205 168 165 154 128 112 91 78
34 9
BbeI G_GCGC'C
Cuts at: 3787 3787
Size: 10891
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3805 7170 7287 928
Size: 971 1906 3365 117 4532
Fragments arranged by size:
4532 3365 1906 971 117
BbvCI CC'TCA_GC
Cuts at: 3736 4153 5175 6501 6938 3736
Size: 417 1022 1326 437 7689
Fragments arranged by size:
7689 1326 1022 437 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4639 4673 9082 9116 1903
Size: 34 2702 34 4409 34 3678
Fragments arranged by size:
4409 3678 2702 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8783 9993 8783
Size: 1210 9681
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10891
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2760 4112 4281
Size: 107 122 71 631 605 1088 1352 169
Cuts at: 4281 4956 4998 7016 9502 136
Size: 675 42 2018 2486 1525
Fragments arranged by size:
2486 2018 1525 1352 1088 675 631 605
169 122 107 71 42
BglII A'GATC_T
Cuts at: 10886 10886
Size: 10891
BlpI GC'TnA_GC
Cuts at: 2793 2960 2969 3939 6333 7223 7365 8308 2793
Size: 167 9 970 2394 890 142 943 5376
Fragments arranged by size:
5376 2394 970 943 890 167 142 9
Bme1580I G_kGCm'C
Cuts at: 1790 2589 2658 2756 2868 3373 4108 4220 4267
Size: 799 69 98 112 505 735 112 47
Cuts at: 4267 5157 5292 5549 8952 10108 1790
Size: 890 135 257 3403 1156 2573
Fragments arranged by size:
3403 2573 1156 890 799 735 505 257
135 112 112 98 69 47
BmgBI CAC'GTC
Cuts at: 1318 2150 5287 1318
Size: 832 3137 6922
Fragments arranged by size:
6922 3137 832
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2621 4136 4840
Size: 416 53 83 399 24 1204 1515 704
Cuts at: 4840 6010 8622 9580 442
Size: 1170 2612 958 1753
Fragments arranged by size:
2612 1753 1515 1204 1170 958 704 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3318 7560 1056
Size: 2262 4242 4387
Fragments arranged by size:
4387 4242 2262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 4168 5020 5962 7945 2488
Size: 1680 852 942 1983 5434
Fragments arranged by size:
5434 1983 1680 942 852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2311 2705 2727 3263 3782 4160 4289
Size: 911 161 394 22 536 519 378 129
Cuts at: 4289 4736 5015 5501 5516 5582 5903 6173 6233
Size: 447 279 486 15 66 321 270 60
Cuts at: 6233 6380 6410 6575 6683 6767 6938 7118 7232
Size: 147 30 165 108 84 171 180 114
Cuts at: 7232 7286 7406 7463 7523 7535 7565 7754 7775
Size: 54 120 57 60 12 30 189 21
Cuts at: 7775 7970 8098 9551 1239
Size: 195 128 1453 2579
Fragments arranged by size:
2579 1453 911 536 519 486 447 394
378 321 279 270 195 189 180 171
165 161 147 129 128 120 114 108
84 66 60 60 57 54 30 30
22 21 15 12
Bpu10I CC'TnA_GC
Cuts at: 2604 3736 4153 4410 5175 5522 6501 6938 7323
Size: 1132 417 257 765 347 979 437 385
Cuts at: 7323 2604
Size: 6172
Fragments arranged by size:
6172 1132 979 765 437 417 385 347
257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2921 4298 4331 6290 7070 7550 7940 9016
Size: 597 1377 33 1959 780 480 390 1076
Cuts at: 9016 9881 10179 2324
Size: 865 298 3036
Fragments arranged by size:
3036 1959 1377 1076 865 780 597 480
390 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5247 9554 882
Size: 617 3748 4307 2219
Fragments arranged by size:
4307 3748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 8188 8682 493
Size: 711 2126 4858 494 2702
Fragments arranged by size:
4858 2702 2126 711 494
BsaBI GATnn'nnATC
Cuts at: 10885 10885
Size: 10891
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 3784 5112 8698
Size: 53 83 186 936 953 1298 1328 3586
Cuts at: 8698 9080 9715 275
Size: 382 635 1451
Fragments arranged by size:
3586 1451 1328 1298 953 936 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5790 9324 9722 9996 10143 10554 5790
Size: 3534 398 274 147 411 6127
Fragments arranged by size:
6127 3534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2159 2189 2252
Size: 30 165 30 687 30 39 30 63
Cuts at: 2252 2282 2658 2688 2867 2897 3062 3092 4133
Size: 30 376 30 179 30 165 30 1041
Cuts at: 4133 4163 4589 4619 7094 7124 7404 7434 8096
Size: 30 426 30 2475 30 280 30 662
Cuts at: 8096 8126 1178
Size: 30 3943
Fragments arranged by size:
3943 2475 1041 687 662 426 376 280
179 165 165 63 39 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2164 2363 3143 4157 4238 4460 4547
Size: 961 17 199 780 1014 81 222 87
Cuts at: 4547 4691 5197 5486 5579 5795 5819 6293 6418
Size: 144 506 289 93 216 24 474 125
Cuts at: 6418 6452 6485 6821 6830 7115 7229 7607 7691
Size: 34 33 336 9 285 114 378 84
Cuts at: 7691 1186
Size: 4386
Fragments arranged by size:
4386 1014 961 780 506 474 378 336
289 285 222 216 199 144 125 114
93 87 84 81 34 33 24 17
9
BseYI C'CCAG_C
Cuts at: 2448 3341 3787 3976 4073 4879 5181 5186 5267
Size: 893 446 189 97 806 302 5 81
Cuts at: 5267 5304 5949 6444 7026 7061 7080 10094 10590
Size: 37 645 495 582 35 19 3014 496
Cuts at: 10590 2448
Size: 2749
Fragments arranged by size:
3014 2749 893 806 645 582 496 495
446 302 189 97 81 37 35 19
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2573 2823 2963 3272 3308 3578
Size: 573 498 339 250 140 309 36 270
Cuts at: 3578 4529 4763 6338 6881 7154 1163
Size: 951 234 1575 543 273 4900
Fragments arranged by size:
4900 1575 951 573 543 498 339 309
273 270 250 234 140 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 4293 8341 9102 10130 1540
Size: 275 186 1237 1055 4048 761 1028 2301
Fragments arranged by size:
4048 2301 1237 1055 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2961 2970 3135 3861 4267 4311 4974 5157
Size: 462 9 165 726 406 44 663 183
Cuts at: 5157 6423 6591 7060 8094 8952 9037 10108 2499
Size: 1266 168 469 1034 858 85 1071 3282
Fragments arranged by size:
3282 1266 1071 1034 858 726 663 469
462 406 183 168 165 85 44 9
BsmI GAATG_Cn'
Cuts at: 4897 7796 8402 8495 4897
Size: 2899 606 93 7293
Fragments arranged by size:
7293 2899 606 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3059 4330 6080 7568 3059
Size: 1271 1750 1488 6382
Fragments arranged by size:
6382 1750 1488 1271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2589 2602 2658 2756 2868 2961
Size: 399 709 90 13 56 98 112 93
Cuts at: 2961 2970 3135 3373 3861 4108 4220 4267 4311
Size: 9 165 238 488 247 112 47 44
Cuts at: 4311 4345 4974 5157 5196 5274 5292 5549 6295
Size: 34 629 183 39 78 18 257 746
Cuts at: 6295 6423 6591 7060 8094 8210 8952 9037 10108
Size: 128 168 469 1034 116 742 85 1071
Cuts at: 10108 1391
Size: 2174
Fragments arranged by size:
2174 1071 1034 746 742 709 629 488
469 399 257 247 238 183 168 165
128 116 112 112 98 93 90 85
78 56 47 44 39 34 18 13
9
BspEI T'CCGG_A
Cuts at: 5790 10554 5790
Size: 4764 6127
BspHI T'CATG_A
Cuts at: 8780 10510 8780
Size: 1730 9161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 2643 3831 5250 6113 6270 6361 7176
Size: 1412 387 1188 1419 863 157 91 815
Cuts at: 7176 7182 7824 8246 844
Size: 6 642 422 3489
Fragments arranged by size:
3489 1419 1412 1188 863 815 642 422
387 157 91 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 2172 2991 4626 6151 6763 7597
Size: 429 278 71 819 1635 1525 612 834
Cuts at: 7597 8778 1394
Size: 1181 3507
Fragments arranged by size:
3507 1635 1525 1181 834 819 612 429
278 71
BsrDI GCAATG_nn'
Cuts at: 65 1248 3682 6546 7152 7608 9386 9560 65
Size: 1183 2434 2864 606 456 1778 174 1396
Fragments arranged by size:
2864 2434 1778 1396 1183 606 456 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4292 4473 5877 6954 7260 9535 9722
Size: 110 2482 181 1404 1077 306 2275 187
Cuts at: 9722 1700
Size: 2869
Fragments arranged by size:
2869 2482 2275 1404 1077 306 187 181
110
BsrGI T'GTAC_A
Cuts at: 96 3627 5683 96
Size: 3531 2056 5304
Fragments arranged by size:
5304 3531 2056
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10891
BssSI C'ACGA_G
Cuts at: 4493 8951 9963 4493
Size: 4458 1012 5421
Fragments arranged by size:
5421 4458 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3295 3556 4961 5152 5511 7399 794
Size: 2501 261 1405 191 359 1888 4286
Fragments arranged by size:
4286 2501 1888 1405 359 261 191
BstBI TT'CG_AA
Cuts at: 2007 9695 2007
Size: 7688 3203
BstEII G'GTnAC_C
Cuts at: 1184 5354 1184
Size: 4170 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2818 3308 3349 4820 5059 2230
Size: 588 490 41 1471 239 8062
Fragments arranged by size:
8062 1471 588 490 239 41
BstYI r'GATC_y
Cuts at: 1972 3247 3696 5491 5658 5787 5808 5892 7251
Size: 1275 449 1795 167 129 21 84 1359
Cuts at: 7251 7741 8221 8588 8973 8990 10442 10528 10540
Size: 490 480 367 385 17 1452 86 12
Cuts at: 10540 10886 1972
Size: 346 1977
Fragments arranged by size:
1977 1795 1452 1359 1275 490 480 449
385 367 346 167 129 86 84 21
17 12
Bsu36I CC'TnA_GG
Cuts at: 3144 3848 3918 3144
Size: 704 70 10117
Fragments arranged by size:
10117 704 70
BtgI C'CryG_G
Cuts at: 513 1065 2362 2649 3364 4429 4819 4965 5311
Size: 552 1297 287 715 1065 390 146 346
Cuts at: 5311 7317 7452 513
Size: 2006 135 3952
Fragments arranged by size:
3952 2006 1297 1065 715 552 390 346
287 146 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2346 3488 3559 4969 7174 487
Size: 683 56 1120 1142 71 1410 2205 4204
Fragments arranged by size:
4204 2205 1410 1142 1120 683 71 56
BtsI GCAGTG_nn'
Cuts at: 3456 3532 4966 5015 5155 5236 5257 5472 8407
Size: 76 1434 49 140 81 21 215 2935
Cuts at: 8407 9201 9221 3456
Size: 794 20 5126
Fragments arranged by size:
5126 2935 1434 794 215 140 81 76
49 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7134 7169 529
Size: 35 6570 35 4251
Fragments arranged by size:
6570 4251 35 35
DraI TTT'AAA
Cuts at: 8195 8195
Size: 10891
DraIII CAC_nnn'GTG
Cuts at: 2872 5371 2872
Size: 2499 8392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9898 809
Size: 507 8582 1802
Fragments arranged by size:
8582 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2403 3108
Size: 54 1425 50 275 18 402 171 705
Cuts at: 3108 3567 3724 3909 3963 4290 5670 6633 8338
Size: 459 157 185 54 327 1380 963 1705
Cuts at: 8338 9227 10856 8
Size: 889 1629 43
Fragments arranged by size:
1705 1629 1425 1380 963 889 705 459
402 327 275 185 171 157 54 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4290 8338 1537
Size: 275 2478 4048 4090
Fragments arranged by size:
4090 4048 2478 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3045 4316 4493 4668 5495 5645 6684 6867
Size: 1692 1271 177 175 827 150 1039 183
Cuts at: 6867 8821 1353
Size: 1954 3423
Fragments arranged by size:
3423 1954 1692 1271 1039 827 183 177
175 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2080 4199 5191 5375 5855 5932
Size: 1064 138 491 2119 992 184 480 77
Cuts at: 5932 9478 9852 9998 387
Size: 3546 374 146 1280
Fragments arranged by size:
3546 2119 1280 1064 992 491 480 374
184 146 138 77
EcoICRI GAG'CTC
Cuts at: 2959 2968 3133 3859 4309 6421 6589 2959
Size: 9 165 726 450 2112 168 7261
Fragments arranged by size:
7261 2112 726 450 168 165 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2311 2705 2727 3263 3782 4089
Size: 393 518 161 394 22 536 519 307
Cuts at: 4089 4160 4289 4736 5015 5501 5516 5582 5903
Size: 71 129 447 279 486 15 66 321
Cuts at: 5903 6050 6161 6173 6233 6380 6410 6536 6575
Size: 147 111 12 60 147 30 126 39
Cuts at: 6575 6683 6767 6938 7016 7067 7118 7217 7232
Size: 108 84 171 78 51 51 99 15
Cuts at: 7232 7286 7406 7463 7523 7535 7565 7754 7775
Size: 54 120 57 60 12 30 189 21
Cuts at: 7775 7970 8098 8954 9551 10338 10606 1239
Size: 195 128 856 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 536 519 518 486
447 394 393 321 307 279 268 195
189 171 161 147 147 129 128 126
120 111 108 99 84 78 71 66
60 60 57 54 51 51 39 30
30 22 21 15 15 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2565 2580 2802 6500 2565
Size: 15 222 3698 6956
Fragments arranged by size:
6956 3698 222 15
EcoO109I rG'GnC_Cy
Cuts at: 2752 2753 2815 2999 3369 3370 4216 4217 4654
Size: 1 62 184 370 1 846 1 437
Cuts at: 4654 5665 5754 6259 6658 6991 7009 7336 7813
Size: 1011 89 505 399 333 18 327 477
Cuts at: 7813 7861 7997 2752
Size: 48 136 5646
Fragments arranged by size:
5646 1011 846 505 477 437 399 370
333 327 184 136 89 62 48 18
1 1 1
EcoRI G'AATT_C
Cuts at: 8200 8578 8200
Size: 378 10513
FseI GG_CCGG'CC
Cuts at: 4296 4296
Size: 10891
FspI TGC'GCA
Cuts at: 5339 9397 5339
Size: 4058 6833
FspAI rTGC'GCAy
Cuts at: 5339 5339
Size: 10891
HaeII r_GCGC'y
Cuts at: 1678 2437 2472 3307 3315 3787 4198 4723 7211
Size: 759 35 835 8 472 411 525 2488
Cuts at: 7211 10038 1678
Size: 2827 2531
Fragments arranged by size:
2827 2531 2488 835 759 525 472 411
35 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2252 2284 2867
Size: 32 135 32 518 32 130 32 583
Cuts at: 2867 2899 3086 3118 3239 3271 3472 3504 4133
Size: 32 187 32 121 32 201 32 629
Cuts at: 4133 4165 4589 4621 5231 5263 5891 5923 6781
Size: 32 424 32 610 32 628 32 858
Cuts at: 6781 6813 6953 6985 7339 7371 7882 7914 7925
Size: 32 140 32 354 32 511 32 11
Cuts at: 7925 7957 8096 8128 9532 9564 9606 9638 1373
Size: 32 139 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1404 858 629 628 610 583 518
511 424 354 201 187 140 139 135
130 121 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 3116 4387 7963 8235 8481
Size: 988 281 694 484 1271 3576 272 246
Cuts at: 8481 669
Size: 3079
Fragments arranged by size:
3576 3079 1271 988 694 484 281 272
246
HindIII A'AGCT_T
Cuts at: 748 5735 748
Size: 4987 5904
HpaI GTT'AAC
Cuts at: 8481 8481
Size: 10891
KasI G'GCGC_C
Cuts at: 3783 3783
Size: 10891
KpnI G_GTAC'C
Cuts at: 8216 8216
Size: 10891
MfeI C'AATT_G
Cuts at: 8490 8490
Size: 10891
MluI A'CGCG_T
Cuts at: 10767 10767
Size: 10891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 4497 10005 10189 1305
Size: 930 2262 5508 184 2007
Fragments arranged by size:
5508 2262 2007 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2405 3110 3569 3726
Size: 54 1425 343 402 171 705 459 157
Cuts at: 3726 3911 5672 6635 10
Size: 185 1761 963 4266
Fragments arranged by size:
4266 1761 1425 963 705 459 402 343
185 171 157 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2738 3330 3444 3971 4179 4270
Size: 648 1062 510 592 114 527 208 91
Cuts at: 4270 4505 4818 5033 5057 5093 5339 5447 6719
Size: 235 313 215 24 36 246 108 1272
Cuts at: 6719 8849 9208 9367 518
Size: 2130 359 159 2042
Fragments arranged by size:
2130 2042 1272 1062 648 592 527 510
359 313 246 235 215 208 159 114
108 91 36 24
MspA1I CmG'CkG
Cuts at: 1606 2111 2238 2380 2548 3543 3588 4431 5011
Size: 505 127 142 168 995 45 843 580
Cuts at: 5011 5313 5508 5572 5773 5818 5953 6089 6241
Size: 302 195 64 201 45 135 136 152
Cuts at: 6241 6289 6448 6463 6478 7096 7222 8307 8984
Size: 48 159 15 15 618 126 1085 677
Cuts at: 8984 10132 10377 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1085 995 843 677 618 580
505 302 245 201 195 168 159 152
142 136 135 127 126 64 48 45
45 15 15
NaeI GCC'GGC
Cuts at: 4294 4294
Size: 10891
NarI GG'CG_CC
Cuts at: 3784 3784
Size: 10891
NcoI C'CATG_G
Cuts at: 513 1065 4819 7452 513
Size: 552 3754 2633 3952
Fragments arranged by size:
3952 3754 2633 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10891
NgoMIV G'CCGG_C
Cuts at: 4292 4292
Size: 10891
NheI G'CTAG_C
Cuts at: 1052 3314 7556 1052
Size: 2262 4242 4387
Fragments arranged by size:
4387 4242 2262
NotI GC'GGCC_GC
Cuts at: 8338 8338
Size: 10891
NspI r_CATG'y
Cuts at: 1132 1229 3406 5038 5344 6493 8249 9794 1132
Size: 97 2177 1632 306 1149 1756 1545 2229
Fragments arranged by size:
2229 2177 1756 1632 1545 1149 306 97
PasI CC'CwG_GG
Cuts at: 1348 2824 1348
Size: 1476 9415
PciI A'CATG_T
Cuts at: 3402 9790 3402
Size: 6388 4503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3553 5668 5956 6388 6724 8055 1108
Size: 2445 2115 288 432 336 1331 3944
Fragments arranged by size:
3944 2445 2115 1331 432 336 288
PfoI T'CCnGG_A
Cuts at: 2295 2676 3186 3636 4713 5661 6591 6654 7440
Size: 381 510 450 1077 948 930 63 786
Cuts at: 7440 7662 2295
Size: 222 5524
Fragments arranged by size:
5524 1077 948 930 786 510 450 381
222 63
PmeI GTTT'AAAC
Cuts at: 8195 8195
Size: 10891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2159 2191 6237 6269 7094 7126 8960 8992 10473
Size: 32 4046 32 825 32 1834 32 1481
Cuts at: 10473 10505 2159
Size: 32 2545
Fragments arranged by size:
4046 2545 1834 1481 825 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2815 2999 5665 6259 6658 6991 7336 7813 7861
Size: 184 2666 594 399 333 345 477 48
Cuts at: 7861 7997 2815
Size: 136 5709
Fragments arranged by size:
5709 2666 594 477 399 345 333 184
136 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10891
PsiI TTA'TAA
Cuts at: 8461 8461
Size: 10891
PspOMI G'GGCC_C
Cuts at: 2752 3369 4104 4216 2752
Size: 617 735 112 9427
Fragments arranged by size:
9427 735 617 112
PstI C_TGCA'G
Cuts at: 830 2379 3960 4136 4194 4482 4911 5280 5621
Size: 1549 1581 176 58 288 429 369 341
Cuts at: 5621 6012 6267 6300 6462 6477 7179 7404 7779
Size: 391 255 33 162 15 702 225 375
Cuts at: 7779 7821 8243 830
Size: 42 422 3478
Fragments arranged by size:
3478 1581 1549 702 429 422 391 375
369 341 288 255 225 176 162 58
42 33 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10891
PvuII CAG'CTG
Cuts at: 2548 3543 5011 5508 5773 5818 5953 6241 7096
Size: 995 1468 497 265 45 135 288 855
Cuts at: 7096 7222 2548
Size: 126 6217
Fragments arranged by size:
6217 1468 995 855 497 288 265 135
126 45
SacI G_AGCT'C
Cuts at: 2961 2970 3135 3861 4311 6423 6591 2961
Size: 9 165 726 450 2112 168 7261
Fragments arranged by size:
7261 2112 726 450 168 165 9
SacII CC_GC'GG
Cuts at: 4432 5314 4432
Size: 882 10009
SalI G'TCGA_C
Cuts at: 1936 2630 4385 8233 1936
Size: 694 1755 3848 4594
Fragments arranged by size:
4594 3848 1755 694
SapI GCTCTTCn'nnn_
Cuts at: 4316 4493 5495 6684 4316
Size: 177 1002 1189 8523
Fragments arranged by size:
8523 1189 1002 177
SbfI CC_TGCA'GG
Cuts at: 4911 5621 6267 7779 7821 8243 4911
Size: 710 646 1512 42 422 7559
Fragments arranged by size:
7559 1512 710 646 422 42
ScaI AGT'ACT
Cuts at: 9139 10851 9139
Size: 1712 9179
SexAI A'CCwGG_T
Cuts at: 6945 6945
Size: 10891
SfcI C'TryA_G
Cuts at: 826 1045 2375 3956 4132 4190 4300 4478 4907
Size: 219 1330 1581 176 58 110 178 429
Cuts at: 4907 5102 5169 5276 5617 6008 6263 6296 6458
Size: 195 67 107 341 391 255 33 162
Cuts at: 6458 6473 7175 7400 7649 7775 7817 8239 9374
Size: 15 702 225 249 126 42 422 1135
Cuts at: 9374 10055 10246 826
Size: 681 191 1471
Fragments arranged by size:
1581 1471 1330 1135 702 681 429 422
391 341 255 249 225 219 195 191
178 176 162 126 110 107 67 58
42 33 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2760 2760
Size: 10891
SfoI GGC'GCC
Cuts at: 3785 3785
Size: 10891
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10891
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10891
SmaI CCC'GGG
Cuts at: 1872 4395 7334 8218 1872
Size: 2523 2939 884 4545
Fragments arranged by size:
4545 2939 2523 884
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 2936 4277 4346 5831 6305
Size: 197 926 396 597 1341 69 1485 474
Cuts at: 6305 7085 7565 7919 8995 9896 10158 820
Size: 780 480 354 1076 901 262 1553
Fragments arranged by size:
1553 1485 1341 1076 926 901 780 597
480 474 396 354 262 197 69
SnaBI TAC'GTA
Cuts at: 493 8188 8682 493
Size: 7695 494 2702
Fragments arranged by size:
7695 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10891
SphI G_CATG'C
Cuts at: 1132 5038 8249 1132
Size: 3906 3211 3774
Fragments arranged by size:
3906 3774 3211
SspI AAT'ATT
Cuts at: 5 52 8815 5
Size: 47 8763 2081
Fragments arranged by size:
8763 2081 47
StuI AGG'CCT
Cuts at: 1517 2768 3056 4004 4604 1517
Size: 1251 288 948 600 7804
Fragments arranged by size:
7804 1251 948 600 288
StyI C'CwwG_G
Cuts at: 513 1065 2811 4819 5350 5550 6615 6636 7005
Size: 552 1746 2008 531 200 1065 21 369
Cuts at: 7005 7452 7671 8004 8330 513
Size: 447 219 333 326 3074
Fragments arranged by size:
3074 2008 1746 1065 552 531 447 369
333 326 219 200 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4481 4901 5318 6575 8067 8918 8935
Size: 1255 2901 420 417 1257 1492 851 17
Cuts at: 8935 9088 9273 9718 325
Size: 153 185 445 1498
Fragments arranged by size:
2901 1498 1492 1257 1255 851 445 420
417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2315 3381 3627
Size: 274 80 33 51 1443 338 1066 246
Cuts at: 3627 4839 5683 5767 9137 10849 96
Size: 1212 844 84 3370 1712 138
Fragments arranged by size:
3370 1712 1443 1212 1066 844 338 274
246 138 84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2535 2567 2658 2690 3062 3094 3142 3174 5073
Size: 32 91 32 372 32 48 32 1899
Cuts at: 5073 5105 10851 10883 2535
Size: 32 5746 32 2543
Fragments arranged by size:
5746 2543 1899 372 91 48 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7967 1316
Size: 6651 4240
XbaI T'CTAG_A
Cuts at: 2536 6577 7744 8227 2536
Size: 4041 1167 483 5200
Fragments arranged by size:
5200 4041 1167 483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 3190 3973 4033 4178 4990 5264 6355 6721
Size: 993 783 60 145 812 274 1091 366
Cuts at: 6721 6742 7012 7207 7678 2197
Size: 21 270 195 471 5410
Fragments arranged by size:
5410 1091 993 812 783 471 366 274
270 195 145 60 21
XhoI C'TCGA_G
Cuts at: 1943 5831 1943
Size: 3888 7003
XmaI C'CCGG_G
Cuts at: 1870 4393 7332 8216 1870
Size: 2523 2939 884 4545
Fragments arranged by size:
4545 2939 2523 884
XmnI GAAnn'nnTTC
Cuts at: 9020 9020
Size: 10891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8699 276
Size: 53 83 186 936 7165 2468
Fragments arranged by size:
7165 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I EcoRV FalI NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SrfI
SwaI