Restriction map for
pFN21ASDA0210
(Circular) (Six-base) MAPSORT of: pFN21ASDA0210.seq Check: 7486 from: 1 to: 7114
pFN21ASDA0210 7114 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3446 3446
Size: 7114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4457 1937
Size: 2520 4594
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 2531 5241 5614 1563
Size: 968 2710 373 3063
Fragments arranged by size:
3063 2710 968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2969 3795 4107 5177 6561 6829 1632
Size: 555 782 826 312 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1070 826 782 555 312 268
AflII C'TTAA_G
Cuts at: 820 1017 3239 820
Size: 197 2222 4695
Fragments arranged by size:
4695 2222 197
AflIII A'CryG_T
Cuts at: 1203 6013 6990 1203
Size: 4810 977 1327
Fragments arranged by size:
4810 1327 977
AgeI A'CCGG_T
Cuts at: 5945 5945
Size: 7114
AhdI GACnn_n'nnGTC
Cuts at: 2092 5843 2092
Size: 3751 3363
AleI CACnn'nnGTG
Cuts at: 1166 4388 1166
Size: 3222 3892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3611 1184
Size: 2427 4687
AlwNI CAG_nnn'CTG
Cuts at: 3571 4857 6429 3571
Size: 1286 1572 4256
Fragments arranged by size:
4256 1572 1286
ApaI G_GGCC'C
Cuts at: 4316 4316
Size: 7114
ApaLI G'TGCA_C
Cuts at: 4138 5171 6327 4138
Size: 1033 1156 4925
Fragments arranged by size:
4925 1156 1033
ApoI r'AATT_y
Cuts at: 1493 2477 2574 3304 3671 3795 3904 3924 4153
Size: 984 97 730 367 124 109 20 229
Cuts at: 4153 4423 4649 4801 6910 1493
Size: 270 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 730 367 270 229 226
152 124 109 97 20
AseI AT'TA_AT
Cuts at: 160 1032 2253 3408 5668 160
Size: 872 1221 1155 2260 1606
Fragments arranged by size:
2260 1606 1221 1155 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 4439 1870
Size: 73 458 2038 4545
Fragments arranged by size:
4545 2038 458 73
BamHI G'GATC_C
Cuts at: 4444 4811 4444
Size: 367 6747
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4435 5890 618
Size: 325 217 627 2648 1455 1842
Fragments arranged by size:
2648 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4211 4316 4433 1391
Size: 2820 105 117 4072
Fragments arranged by size:
4072 2820 117 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6143
BbvCI CC'TCA_GC
Cuts at: 3101 3520 4321 3101
Size: 419 801 5894
Fragments arranged by size:
5894 801 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5305 5339 1903
Size: 34 3368 34 3678
Fragments arranged by size:
3678 3368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 2433 1548
Size: 885 6229
BfrBI ATG'CAT
Cuts at: 3202 3202
Size: 7114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5725 136
Size: 107 122 71 631 605 4053 1525
Fragments arranged by size:
4053 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7109 7109
Size: 7114
BlpI GC'TnA_GC
Cuts at: 4531 4531
Size: 7114
Bme1580I G_kGCm'C
Cuts at: 1790 4142 4316 5175 6331 1790
Size: 2352 174 859 1156 2573
Fragments arranged by size:
2573 2352 1156 859 174
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2715 4268 4845
Size: 416 53 83 399 24 1298 1553 577
Cuts at: 4845 5803 442
Size: 958 1753
Fragments arranged by size:
1753 1553 1298 958 577 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3353
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 3429 3492 3681 4036 4315 4330 5774
Size: 897 1293 63 189 355 279 15 1444
Cuts at: 5774 1239
Size: 2579
Fragments arranged by size:
2579 1444 1293 897 355 279 189 63
15
Bpu10I CC'TnA_GC
Cuts at: 3101 3520 4321 3101
Size: 419 801 5894
Fragments arranged by size:
5894 801 419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2304 5239 6104 6402 2304
Size: 2935 865 298 3016
Fragments arranged by size:
3016 2935 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3397 5777 882
Size: 617 1898 2380 2219
Fragments arranged by size:
2380 2219 1898 617
BsaAI yAC'GTr
Cuts at: 493 1204 4411 4905 493
Size: 711 3207 494 2702
Fragments arranged by size:
3207 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4921 5303 5938 275
Size: 53 83 186 936 3388 382 635 1451
Fragments arranged by size:
3388 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5547 5945 6219 6366 6777 5547
Size: 398 274 147 411 5884
Fragments arranged by size:
5884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3667 3697 4034 4064 4328
Size: 30 165 30 2264 30 337 30 264
Cuts at: 4328 4358 1178
Size: 30 3934
Fragments arranged by size:
3934 2264 337 264 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2133 3342 3481 3484 1186
Size: 947 1209 139 3 4816
Fragments arranged by size:
4816 1209 947 139 3
BseYI C'CCAG_C
Cuts at: 3433 4227 4315 4340 6317 6813 3433
Size: 794 88 25 1977 496 3734
Fragments arranged by size:
3734 1977 794 496 88 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2697 3474 1163
Size: 573 961 777 4803
Fragments arranged by size:
4803 961 777 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4564 5325 6353 1540
Size: 275 186 2563 761 1028 2301
Fragments arranged by size:
2563 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3373 4142 5175 5260 6331 3373
Size: 769 1033 85 1071 4156
Fragments arranged by size:
4156 1071 1033 769 85
BsmI GAATG_Cn'
Cuts at: 4625 4718 4625
Size: 93 7021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3373 4142 4211 4316 4433 5175 5260
Size: 399 1583 769 69 105 117 742 85
Cuts at: 5260 6331 1391
Size: 1071 2174
Fragments arranged by size:
2174 1583 1071 769 742 399 117 105
85 69
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 3339 5003 6733 3339
Size: 1664 1730 3720
Fragments arranged by size:
3720 1730 1664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 3446 4469 844
Size: 1421 1181 1023 3489
Fragments arranged by size:
3489 1421 1181 1023
BsrBI CCG'CTC
Cuts at: 1394 1823 5001 1394
Size: 429 3178 3507
Fragments arranged by size:
3507 3178 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3444 4029 5609 5783 65
Size: 1183 2196 585 1580 174 1396
Fragments arranged by size:
2196 1580 1396 1183 585 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5758 5945 1700
Size: 110 3948 187 2869
Fragments arranged by size:
3948 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3223 96
Size: 3127 3987
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 3324 5174 6186 3324
Size: 1850 1012 4252
Fragments arranged by size:
4252 1850 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2289 794
Size: 1495 5619
BstBI TT'CG_AA
Cuts at: 2007 5918 2007
Size: 3911 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstYI r'GATC_y
Cuts at: 1972 2931 3259 4300 4444 4811 5196 5213 6665
Size: 959 328 1041 144 367 385 17 1452
Cuts at: 6665 6751 6763 7109 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1041 959 385 367 346 328
144 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6375
Fragments arranged by size:
6375 683 56
BtsI GCAGTG_nn'
Cuts at: 2784 3457 4204 4630 5424 5444 2784
Size: 673 747 426 794 20 4454
Fragments arranged by size:
4454 794 747 673 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7079
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6121 809
Size: 507 4805 1802
Fragments arranged by size:
4805 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2416 4057 4219
Size: 54 1425 50 275 18 586 1641 162
Cuts at: 4219 4561 5450 7079 8
Size: 342 889 1629 43
Fragments arranged by size:
1641 1629 1425 889 586 342 275 162
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4561 1537
Size: 275 2749 4090
Fragments arranged by size:
4090 2749 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 4005 4126 4239 5044 1353
Size: 2308 344 121 113 805 3423
Fragments arranged by size:
3423 2308 805 344 121 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5701 6075 6221 387
Size: 1064 138 4112 374 146 1280
Fragments arranged by size:
4112 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2187 2969 3429 3492 3681 3795
Size: 393 504 51 782 460 63 189 114
Cuts at: 3795 4036 4107 4315 4330 5177 5774 6561 6829
Size: 241 71 208 15 847 597 787 268
Cuts at: 6829 1239
Size: 1524
Fragments arranged by size:
1524 847 787 782 597 504 460 393
268 241 208 189 114 71 63 51
15
EcoO109I rG'GnC_Cy
Cuts at: 3061 3516 3529 4270 3061
Size: 455 13 741 5905
Fragments arranged by size:
5905 741 455 13
EcoRI G'AATT_C
Cuts at: 3671 4153 4423 4801 3671
Size: 482 270 378 5984
Fragments arranged by size:
5984 482 378 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2172 2483 2172
Size: 311 6803
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 6261 1678
Size: 4583 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2425 2457 2923
Size: 32 135 32 506 32 315 32 466
Cuts at: 2923 2955 3212 3244 3850 3882 5755 5787 5829
Size: 32 257 32 606 32 1873 32 42
Cuts at: 5829 5861 1373
Size: 32 2626
Fragments arranged by size:
2626 1873 606 506 466 315 257 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2926 4458 4704 669
Size: 988 281 988 1532 246 3079
Fragments arranged by size:
3079 1532 988 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2688 748
Size: 1940 5174
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 2904 3599 4713 2904
Size: 695 1114 5305
Fragments arranged by size:
5305 1114 695
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6228 6412 1305
Size: 4923 184 2007
Fragments arranged by size:
4923 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2418 10
Size: 54 1425 343 586 4706
Fragments arranged by size:
4706 1425 586 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2703 3593 4388 5072 5431 5590 518
Size: 648 1537 890 795 684 359 159 2042
Fragments arranged by size:
2042 1537 890 795 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 4056 4530 5207 6355 6600 1606
Size: 657 1793 474 677 1148 245 2120
Fragments arranged by size:
2120 1793 1148 677 657 474 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6562
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NsiI A_TGCA'T
Cuts at: 3204 3204
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2641 2702 2977 3592 4472 6017 1132
Size: 97 1412 61 275 615 880 1545 2229
Fragments arranged by size:
2229 1545 1412 880 615 275 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7114
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7114
PfoI T'CCnGG_A
Cuts at: 2934 2934
Size: 7114
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5183 5215 6696 6728 5183
Size: 32 1481 32 5569
Fragments arranged by size:
5569 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3061 3516 3061
Size: 455 6659
PshAI GACnn'nnGTC
Cuts at: 1613 2328 1613
Size: 715 6399
PsiI TTA'TAA
Cuts at: 2628 4684 2628
Size: 2056 5058
PspOMI G'GGCC_C
Cuts at: 4312 4312
Size: 7114
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2137 2169 2137
Size: 32 7082
PstI C_TGCA'G
Cuts at: 830 2061 3060 4466 830
Size: 1231 999 1406 3478
Fragments arranged by size:
3478 1406 1231 999
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 2263 2263
Size: 7114
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SbfI CC_TGCA'GG
Cuts at: 3060 4466 3060
Size: 1406 5708
ScaI AGT'ACT
Cuts at: 5362 7074 5362
Size: 1712 5402
SfcI C'TryA_G
Cuts at: 826 1045 2057 2185 2658 3056 4462 5597 6278
Size: 219 1012 128 473 398 1406 1135 681
Cuts at: 6278 6469 826
Size: 191 1471
Fragments arranged by size:
1471 1406 1135 1012 681 473 398 219
191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 4441 1872
Size: 2569 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2319 3239 5218 6119 6381 820
Size: 197 926 376 920 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 920 901 376 262 197
SnaBI TAC'GTA
Cuts at: 493 4411 4905 493
Size: 3918 494 2702
Fragments arranged by size:
3918 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 2641 3592 4472 1132
Size: 1509 951 880 3774
Fragments arranged by size:
3774 1509 951 880
SspI AAT'ATT
Cuts at: 5 52 3880 5038 5
Size: 47 3828 1158 2081
Fragments arranged by size:
3828 2081 1158 47
StuI AGG'CCT
Cuts at: 1517 4118 1517
Size: 2601 4513
StyI C'CwwG_G
Cuts at: 513 1065 2810 4078 4553 513
Size: 552 1745 1268 475 3074
Fragments arranged by size:
3074 1745 1268 552 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3013 5141 5158 5311 5496 5941 325
Size: 1255 1433 2128 17 153 185 445 1498
Fragments arranged by size:
2128 1498 1433 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3223 5360 7072
Size: 274 80 33 51 1443 1246 2137 1712
Cuts at: 7072 96
Size: 138
Fragments arranged by size:
2137 1712 1443 1246 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3667 3699 3821 3853 4034 4066 7074 7106 3667
Size: 32 122 32 181 32 3008 32 3675
Fragments arranged by size:
3675 3008 181 122 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7114
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 4439 1870
Size: 2569 4545
XmnI GAAnn'nnTTC
Cuts at: 2149 2477 5243 2149
Size: 328 2766 4020
Fragments arranged by size:
4020 2766 328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI