Restriction map for
pFN21ASDA0207
(Circular) (Six-base) MAPSORT of: pFN21ASDA0207.seq Check: 4742 from: 1 to: 6493
pFN21ASDA0207 6493 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3009 3009
Size: 6493
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2357 3652 4556 5940 6208 1632
Size: 633 92 1295 904 1384 268 1917
Fragments arranged by size:
1917 1384 1295 904 633 268 92
AfeI AGC'GCT
Cuts at: 2336 2336
Size: 6493
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 3331 5392 6369 1203
Size: 2128 2061 977 1327
Fragments arranged by size:
2128 2061 1327 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6493
AlwNI CAG_nnn'CTG
Cuts at: 3010 4236 5808 3010
Size: 1226 1572 3695
Fragments arranged by size:
3695 1572 1226
ApaLI G'TGCA_C
Cuts at: 2348 4550 5706 2348
Size: 2202 1156 3135
Fragments arranged by size:
3135 2202 1156
ApoI r'AATT_y
Cuts at: 1493 2729 2782 2851 2880 3625 3692 3802 4028
Size: 1236 53 69 29 745 67 110 226
Cuts at: 4028 4180 6289 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1236 745 226 152 110 69
67 53 29
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 2317 3818 1870
Size: 73 331 43 1501 4545
Fragments arranged by size:
4545 1501 331 73 43
AvrII C'CTAG_G
Cuts at: 2656 3418 2656
Size: 762 5731
BamHI G'GATC_C
Cuts at: 3823 4190 3823
Size: 367 6126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2367 2811 3814 5269
Size: 325 217 627 446 134 444 1003 1455
Cuts at: 5269 618
Size: 1842
Fragments arranged by size:
1842 1455 1003 627 446 444 325 217
134
BanII G_rGCy'C
Cuts at: 1391 2456 3090 3540 3812 1391
Size: 1065 634 450 272 4072
Fragments arranged by size:
4072 1065 634 450 272
BbeI G_GCGC'C
Cuts at: 2371 2371
Size: 6493
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2388 2685 928
Size: 971 489 297 4736
Fragments arranged by size:
4736 971 489 297
BbvCI CC'TCA_GC
Cuts at: 3131 3234 3131
Size: 103 6390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2057 2091 2165 2199 3256 3290 4684
Size: 34 120 34 74 34 1057 34 1394
Cuts at: 4684 4718 1903
Size: 34 3678
Fragments arranged by size:
3678 1394 1057 120 74 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6493
BfrBI ATG'CAT
Cuts at: 2186 2186
Size: 6493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5104 136
Size: 107 122 71 631 605 3432 1525
Fragments arranged by size:
3432 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2951 3633 6488 2951
Size: 682 2855 2956
Fragments arranged by size:
2956 2855 682
BlpI GC'TnA_GC
Cuts at: 3910 3910
Size: 6493
Bme1580I G_kGCm'C
Cuts at: 1790 2238 2352 4554 5710 1790
Size: 448 114 2202 1156 2573
Fragments arranged by size:
2573 2202 1156 448 114
BmgBI CAC'GTC
Cuts at: 1318 3168 1318
Size: 1850 4643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4224 5182 442
Size: 416 53 83 399 24 2807 958 1753
Fragments arranged by size:
2807 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2848 3041 3392 3409 3484 5153 1239
Size: 974 635 193 351 17 75 1669 2579
Fragments arranged by size:
2579 1669 974 635 351 193 75 17
Bpu10I CC'TnA_GC
Cuts at: 3131 3234 3131
Size: 103 6390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3179 3743 4618 5483 5781 3179
Size: 564 875 865 298 3891
Fragments arranged by size:
3891 875 865 564 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2571 5156 882
Size: 617 1072 2585 2219
Fragments arranged by size:
2585 2219 1072 617
BsaAI yAC'GTr
Cuts at: 493 1204 3790 4284 493
Size: 711 2586 494 2702
Fragments arranged by size:
2702 2586 711 494
BsaBI GATnn'nnATC
Cuts at: 3413 6487 3413
Size: 3074 3419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2368 3269 4300 4682
Size: 53 83 186 936 835 901 1031 382
Cuts at: 4682 5317 275
Size: 635 1451
Fragments arranged by size:
1451 1031 936 901 835 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4926 5324 5598 5745 6156 4926
Size: 398 274 147 411 5263
Fragments arranged by size:
5263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 2186 2216 3023
Size: 30 165 30 688 30 65 30 807
Cuts at: 3023 3053 1178
Size: 30 4618
Fragments arranged by size:
4618 807 688 165 65 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2280 3182 3389 3548 1186
Size: 1094 902 207 159 4131
Fragments arranged by size:
4131 1094 902 207 159
BseYI C'CCAG_C
Cuts at: 2340 2838 2908 5696 6192 2340
Size: 498 70 2788 496 2641
Fragments arranged by size:
2788 2641 498 496 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 3165 1163
Size: 573 615 814 4491
Fragments arranged by size:
4491 814 615 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3943 4704 5732 1540
Size: 275 186 1942 761 1028 2301
Fragments arranged by size:
2301 1942 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2352 2466 2646 4554 4639 5710 2352
Size: 114 180 1908 85 1071 3135
Fragments arranged by size:
3135 1908 1071 180 114 85
BsmI GAATG_Cn'
Cuts at: 2188 3210 4004 4097 2188
Size: 1022 794 93 4584
Fragments arranged by size:
4584 1022 794 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2352 2456 2466 2646 3090 3540
Size: 399 448 114 104 10 180 444 450
Cuts at: 3540 3812 4554 4639 5710 1391
Size: 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 448 444 399 272
180 114 104 85 10
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3009 3848 844
Size: 2165 839 3489
Fragments arranged by size:
3489 2165 839
BsrBI CCG'CTC
Cuts at: 1394 1823 4380 1394
Size: 429 2557 3507
Fragments arranged by size:
3507 2557 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3312 4988 5162 65
Size: 1183 2064 1676 174 1396
Fragments arranged by size:
2064 1676 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2410 5137 5324 1700
Size: 110 223 377 2727 187 2869
Fragments arranged by size:
2869 2727 377 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 3299 3539 4553 5565 3299
Size: 240 1014 1012 4227
Fragments arranged by size:
4227 1014 1012 240
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 794
Size: 1535 4958
BstBI TT'CG_AA
Cuts at: 2007 5297 2007
Size: 3290 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6493
BstYI r'GATC_y
Cuts at: 1972 2951 3495 3633 3823 4190 4575 4592 6044
Size: 979 544 138 190 367 385 17 1452
Cuts at: 6044 6130 6142 6488 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 979 544 385 367 346 190
138 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2712 3082 513
Size: 552 1647 370 3924
Fragments arranged by size:
3924 1647 552 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5754
Fragments arranged by size:
5754 683 56
BtsI GCAGTG_nn'
Cuts at: 3286 3766 4009 4803 4823 3286
Size: 480 243 794 20 4956
Fragments arranged by size:
4956 794 480 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2526 2561 529
Size: 35 1962 35 4461
Fragments arranged by size:
4461 1962 35 35
DraI TTT'AAA
Cuts at: 2770 3797 2770
Size: 1027 5466
DraIII CAC_nnn'GTG
Cuts at: 2614 3603 2614
Size: 989 5504
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 2412 2715
Size: 54 1425 50 275 18 421 161 303
Cuts at: 2715 3012 3940 4829 6458 8
Size: 297 928 889 1629 43
Fragments arranged by size:
1629 1425 928 889 421 303 297 275
161 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3940 1537
Size: 275 2128 4090
Fragments arranged by size:
4090 2128 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4423 1353
Size: 3070 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3365 5080 5454 5600 387
Size: 1064 138 1776 1715 374 146 1280
Fragments arranged by size:
1776 1715 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2265 2357 2848 3041 3392 3409
Size: 393 581 52 92 491 193 351 17
Cuts at: 3409 3484 3652 4556 5153 5940 6208 1239
Size: 75 168 904 597 787 268 1524
Fragments arranged by size:
1524 904 787 597 581 491 393 351
268 193 168 92 75 52 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 3374 2375
Size: 999 5494
EcoO109I rG'GnC_Cy
Cuts at: 2285 2285
Size: 6493
EcoRI G'AATT_C
Cuts at: 3802 4180 3802
Size: 378 6115
FspI TGC'GCA
Cuts at: 3278 4999 3278
Size: 1721 4772
HaeII r_GCGC'y
Cuts at: 1678 2338 2371 5640 1678
Size: 660 33 3269 2531
Fragments arranged by size:
3269 2531 660 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 3328 3360 5134
Size: 32 135 32 519 32 1205 32 1774
Cuts at: 5134 5166 5208 5240 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1774 1205 519 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2084 3718 3837 4083 669
Size: 988 281 146 1634 119 246 3079
Fragments arranged by size:
3079 1634 988 281 246 146 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KasI G'GCGC_C
Cuts at: 2367 2367
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 2588 4092 2588
Size: 1504 4989
MluI A'CGCG_T
Cuts at: 3331 6369 3331
Size: 3038 3455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5607 5791 1305
Size: 4302 184 2007
Fragments arranged by size:
4302 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 2717 10
Size: 54 1425 343 421 464 3786
Fragments arranged by size:
3786 1425 464 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3776 4451 4810 4969 518
Size: 648 2241 369 675 359 159 2042
Fragments arranged by size:
2241 2042 675 648 369 359 159
MspA1I CmG'CkG
Cuts at: 1606 2459 2628 3007 3712 3730 3909 4586 5734
Size: 853 169 379 705 18 179 677 1148
Cuts at: 5734 5979 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 853 705 677 379 245 179
169 18
NaeI GCC'GGC
Cuts at: 2035 2412 2035
Size: 377 6116
NarI GG'CG_CC
Cuts at: 2368 2368
Size: 6493
NcoI C'CATG_G
Cuts at: 513 1065 2712 513
Size: 552 1647 4294
Fragments arranged by size:
4294 1647 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6493
NgoMIV G'CCGG_C
Cuts at: 2033 2410 2033
Size: 377 6116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NsiI A_TGCA'T
Cuts at: 2188 2188
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 2221 2261 2900 3851 5396 1132
Size: 97 992 40 639 951 1545 2229
Fragments arranged by size:
2229 1545 992 951 639 97 40
PasI CC'CwG_GG
Cuts at: 1348 2447 1348
Size: 1099 5394
PciI A'CATG_T
Cuts at: 5392 5392
Size: 6493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2593 2689 1108
Size: 1485 96 4912
Fragments arranged by size:
4912 1485 96
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2421 4562 4594 6075 6107 2389
Size: 32 2141 32 1481 32 2775
Fragments arranged by size:
2775 2141 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PstI C_TGCA'G
Cuts at: 830 2216 2247 3006 3845 830
Size: 1386 31 759 839 3478
Fragments arranged by size:
3478 1386 839 759 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2628 3007 3712 3730 2628
Size: 379 705 18 5391
Fragments arranged by size:
5391 705 379 18
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 4741 6453 4741
Size: 1712 4781
SfcI C'TryA_G
Cuts at: 826 1045 2212 2243 3002 3075 3841 4976 5657
Size: 219 1167 31 759 73 766 1135 681
Cuts at: 5657 5848 826
Size: 191 1471
Fragments arranged by size:
1471 1167 1135 766 759 681 219 191
73 31
SfoI GGC'GCC
Cuts at: 2369 2369
Size: 6493
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3194 3758 4597 5498 5760 820
Size: 197 926 1251 564 839 901 262 1553
Fragments arranged by size:
1553 1251 926 901 839 564 262 197
SnaBI TAC'GTA
Cuts at: 493 3790 4284 493
Size: 3297 494 2702
Fragments arranged by size:
3297 2702 494
SpeI A'CTAG_T
Cuts at: 152 2635 152
Size: 2483 4010
SphI G_CATG'C
Cuts at: 1132 2221 2261 3851 1132
Size: 1089 40 1590 3774
Fragments arranged by size:
3774 1590 1089 40
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 3248 1517
Size: 1731 4762
StyI C'CwwG_G
Cuts at: 513 1065 2656 2712 2973 3418 3932 513
Size: 552 1591 56 261 445 514 3074
Fragments arranged by size:
3074 1591 552 514 445 261 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 2283 4520 4537 4690 4875 5320
Size: 1255 559 144 2237 17 153 185 445
Cuts at: 5320 325
Size: 1498
Fragments arranged by size:
2237 1498 1255 559 445 185 153 144
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3063 3455
Size: 274 80 33 51 1443 225 861 392
Cuts at: 3455 3592 4739 6451 96
Size: 137 1147 1712 138
Fragments arranged by size:
1712 1443 1147 861 392 274 225 138
137 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6453 6485 6453
Size: 32 6461
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2822 2822
Size: 6493
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 2786 4622 2786
Size: 1836 4657
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BplI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI